Pesticidal genes and methods of use

ABSTRACT

Compositions having pesticidal activity and methods for their use are provided. Compositions include isolated and recombinant polypeptide sequences having pesticidal activity, recombinant and synthetic nucleic acid molecules encoding the pesticidal polypeptides, DNA constructs comprising the nucleic acid molecules, vectors comprising the nucleic acid molecules, host cells comprising the vectors, and antibodies to the pesticidal polypeptides. Nucleotide sequences encoding the polypeptides provided herein can be used in DNA constructs or expression cassettes for transformation and expression in organisms of interest. The compositions and methods provided herein are useful for the production of organisms with enhanced pest resistance or tolerance. Transgenic plants and seeds comprising a nucleotide sequence that encodes a pesticidal protein of the invention are also provided. Methods are provided for producing the polypeptides disclosed herein, and for using those polypeptides for controlling a pest. Methods and kits for detecting polypeptides of the invention in a sample are also included.

FIELD OF THE INVENTION

The invention is drawn to methods and compositions for controllingpests, particularly plant pests.

BACKGROUND

Pests, plant diseases, and weeds can be serious threats to crops. Lossesdue to pests and diseases have been estimated at 37% of the agriculturalproduction worldwide, with 13% due to insects, bacteria and otherorganisms.

Toxins are virulence determinants that play an important role inmicrobial pathogenicity and/or evasion of the host immune response.Toxins from the gram-positive bacterium Bacillus, particularly Bacillusthuringiensis, have been used as insecticidal proteins. Currentstrategies use the genes expressing these toxins to produce transgeniccrops. Transgenic crops expressing insecticidal protein toxins are usedto combat crop damage from insects.

While the use of Bacillus toxins has been successful in controllinginsects, resistance to Bt toxins has developed in some target pests inmany parts of the world where such toxins have been used intensively.One way of solving this problem is sowing Bt crops with alternating rowsof regular non Bt crops (refuge). An alternative method to avoid or slowdown development of insect resistance is stacking insecticidal geneswith different modes of action against insects in transgenic plants. Thecurrent strategy of using transgenic crops expressing insecticidalprotein toxins is placing increasing emphasis on the discovery of noveltoxins, beyond those already derived from the bacterium Bacillusthuringiensis. These toxins may prove useful as alternatives to thosederived from B. thuringiensis for deployment in insect- andpest-resistant transgenic plants. Thus, new toxin proteins are needed.

SUMMARY

Compositions having pesticidal activity and methods for their use areprovided. Compositions include isolated and recombinant polypeptidesequences having pesticidal activity, recombinant and synthetic nucleicacid molecules encoding the pesticidal polypeptides, DNA constructscomprising the nucleic acid molecules, vectors comprising the nucleicacid molecules, host cells comprising the vectors, and antibodies to thepesticidal polypeptides. Nucleotide sequences encoding the polypeptidesprovided herein can be used in DNA constructs or expression cassettesfor transformation and expression in organisms of interest, includingmicroorganisms and plants.

The compositions and methods provided herein are useful for theproduction of organisms with enhanced pest resistance or tolerance.These organisms and compositions comprising the organisms are desirablefor agricultural purposes. Transgenic plants and seeds comprising anucleotide sequence that encodes a pesticidal protein of the inventionare also provided. Such plants are resistant to insects and other pests.

Methods are provided for producing the various polypeptides disclosedherein, and for using those polypeptides for controlling or killing apest. Methods and kits for detecting polypeptides of the invention in asample are also included.

DETAILED DESCRIPTION OF THE INVENTION

The present inventions now will be described more fully hereinafter withreference to the accompanying drawings, in which some, but not allembodiments of the inventions are shown. Indeed, these inventions may beembodied in many different forms and should not be construed as limitedto the embodiments set forth herein; rather, these embodiments areprovided so that this disclosure will satisfy applicable legalrequirements. Like numbers refer to like elements throughout.

Many modifications and other embodiments of the inventions set forthherein will come to mind to one skilled in the art to which theseinventions pertain having the benefit of the teachings presented in theforegoing descriptions and the associated drawings. Therefore, it is tobe understood that the inventions are not to be limited to the specificembodiments disclosed and that modifications and other embodiments areintended to be included within the scope of the appended claims.Although specific terms are employed herein, they are used in a genericand descriptive sense only and not for purposes of limitation.

I. Polynucleotides and Polypeptides

Compositions and method for conferring pesticidal activity to anorganism are provided. The modified organism exhibits pesticidalresistance or tolerance. Recombinant pesticidal proteins, orpolypeptides and fragments and variants thereof that retain pesticidalactivity, are provided and include those set forth in SEQ ID NOs: 1, 2,3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22,23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40,41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58,59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76,77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94,95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109,110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123,124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137,138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151,152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165,166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179,180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193,194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207,208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221,222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235,236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249,250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263,264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277,278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291,292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305,306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319,320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333,334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347,348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361,362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375,376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389,390, 391, 392, 393, 394, 395, 396, 397, 398, 399, 400, 401, 402, 403,404, 405, 406, 407, 408, 409, 410, 411, 412, 413, 414, 415, 416, 417,418, 419, 420, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431,432, 433, 434, 435, 436, 437, 438, 439, 440, 441, 442, 443, 444, 445,446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459,460, 461, 462, 463, 464, 465, 466, 467, 468, 469, 470, 471, 472, 473,474, 475, 476, 477, 478, 479, 480, 481, 482, 483, 484, 485, 486, 487,488, 489, 490, 491, 492, 493, 494, 495, 496, 497, 498, 499, 500, 501,502, 503, 504, 505, 506, 507, 508, 509, 510, 511, 512, 513, 514, 515,516, 517, 518, 519, 520, 521, 522, 523, 524, and/or 525. The pesticidalproteins are biologically active (e.g., pesticidal) against pestsincluding insects, fungi, nematodes, and the like. Nucleotides encodingthe pesticidal polypeptides, including for example, SEQ ID NOs: 1, 2, 3,4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22,23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40,41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58,59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76,77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94,95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109,110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123,124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137,138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151,152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165,166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179,180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193,194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207,208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221,222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235,236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249,250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263,264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277,278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291,292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305,306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319,320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333,334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347,348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361,362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375,376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389,390, 391, 392, 393, 394, 395, 396, 397, 398, 399, 400, 401, 402, 403,404, 405, 406, 407, 408, 409, 410, 411, 412, 413, 414, 415, 416, 417,418, 419, 420, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431,432, 433, 434, 435, 436, 437, 438, 439, 440, 441, 442, 443, 444, 445,446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459,460, 461, 462, 463, 464, 465, 466, 467, 468, 469, 470, 471, 472, 473,474, 475, 476, 477, 478, 479, 480, 481, 482, 483, 484, 485, 486, 487,488, 489, 490, 491, 492, 493, 494, 495, 496, 497, 498, 499, 500, 501,502, 503, 504, 505, 506, 507, 508, 509, 510, 511, 512, 513, 514, 515,516, 517, 518, 519, 520, 521, 522, 523, 524, and/or 525, or activefragments or variants thereof, can be used to produce transgenicorganisms, such as plants and microorganisms. The pesticidal proteinsare biologically active (for example, are pesticidal) against pestsincluding insects, fungi, nematodes, and the like. In specificembodiments, the pesticidal polypeptides and the active variant andfragments thereof have an improved pesticidal activity when compared toother polypeptides in the art. Polynucleotides encoding the pesticidalpolypeptides, including for example, SEQ ID NOs: 1, 2, 3, 4, 5, 6, 7, 8,9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26,27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44,45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62,63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80,81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98,99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112,113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126,127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140,141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154,155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168,169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182,183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196,197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210,211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224,225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238,239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252,253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266,267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280,281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294,295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308,309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322,323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336,337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350,351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364,365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378,379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392,393, 394, 395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406,407, 408, 409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420,421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434,435, 436, 437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448,449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 461, 462,463, 464, 465, 466, 467, 468, 469, 470, 471, 472, 473, 474, 475, 476,477, 478, 479, 480, 481, 482, 483, 484, 485, 486, 487, 488, 489, 490,491, 492, 493, 494, 495, 496, 497, 498, 499, 500, 501, 502, 503, 504,505, 506, 507, 508, 509, 510, 511, 512, 513, 514, 515, 516, 517, 518,519, 520, 521, 522, 523, 524, and/or 525, or active fragments orvariants thereof, can be used to produce transgenic organisms, such asplants and microorganisms. The transformed organisms are characterizedby genomes that comprise at least one stably incorporated DNA constructcomprising a coding sequence for a pesticidal protein disclosed herein.In some embodiments, the coding sequence is operably linked to apromoter that drives expression of the encoded pesticidal polypeptide.Accordingly, transformed microorganisms, plant cells, plant tissues,plants, seeds, and plant parts are provided. A summary of variouspolypeptides, active variants and fragments thereof, and polynucleotidesencoding the same are set forth below in Table 1. As noted in Table 1,various forms of polypeptides are provided. Full length pesticidalpolypeptides, as well as, modified versions of the original full-lengthsequence (i.e., variants) are provided. Table 1 further denotes “CryBP1”sequences. Such sequences (SEQ ID NOs: 3, 9, 57, 143, 220, 365, 404, and449) comprise accessory polypeptides that can be associated with some ofthe toxin genes. In such instances, the CryBP1 sequences can be usedalone or in combination with any of the pesticidal polypeptides providedherein. Table 1 further provides Split-Cry C-terminus polypeptides (SEQID NO: 27, 48, 85, 139, 271, 374, 430, and 497). Such sequences comprisethe sequence of a downstream protein that has homology to the C-terminalend of the Cry class of toxin genes and are usually found after a Crygene that is not full-length and is missing the expected C-terminalregion.

TABLE 1 Summary of SEQ ID NOs, Gene Class, and Variants thereof Split-Modified Cry C- Seq Seq CryBP1 terminus Gene APG ID ID ID(s) Seq ID SeqID Class % Identity APG00751.0 1 2 3 Cry 55, 60, 65, 70, 75, 80, 85, 90,91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG00831.0 4 5, 6 Cry 60, 65,70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG00832.0 78 9 Cry 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96,97, 98, 99, 100 APG00842.0 10 11 MTX 5, 10, 15, 20, 25, 30, 35, 40, 45,50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99,100 APG00887.0 12 13 MTX 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100APG00961.0 14 15 Cry 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100APG00973.0 16 17, 18 MTX 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 91,92, 93, 94, 95, 96, 97, 98, 99, 100 APG01038.0 19 20 MTX 65, 70, 75, 80,85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG01063.0 21 22 Bin 75,80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG01092.0 23 24 Cry60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100APG01102.0 25 26 27 Cry 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97,98, 99, 100 APG01127.0 28 MTX 95, 96, 97, 98, 99, 100 APG01141.0 29 30Bin 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG01163.0 31 32 MTX50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99,100 APG01165.0 33 34 Cry 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97,98, 99, 100 APG01201.0 35 36 MTX 50, 55, 60, 65, 70, 75, 80, 85, 90, 91,92, 93, 94, 95, 96, 97, 98, 99, 100 APG01281.0 37 38 MTX 65, 70, 75, 80,85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG01352.0 39 40 Bin 50,55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100APG01380.0 41 Cry34 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65,70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG01385.042 MTX 97, 98, 99, 100 APG01396.0 43 44 MTX 70, 75, 80, 85, 90, 91, 92,93, 94, 95, 96, 97, 98, 99, 100 APG01434.0 45 46, 47 48 Cry 85, 90, 91,92, 93, 94, 95, 96, 97, 98, 99, 100 APG01455.0 49 MTX 97, 98, 99, 100APG01516.0 50 51 MTX 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99,100 APG01549.0 52 MTX 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94,95, 96, 97, 98, 99, 100 APG01617.0 53 54 MTX 85, 90, 91, 92, 93, 94, 95,96, 97, 98, 99, 100 APG01681.0 55 56 57 Bin 40, 45, 50, 55, 60, 65, 70,75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG01686.0 58 59Cry 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99,100 APG01710.0 60 61 Cry 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100APG01788.0 62 63 Cry 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92,93, 94, 95, 96, 97, 98, 99, 100 APG01803.0 64 65 Cry 35, 40, 45, 50, 55,60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100APG01810.0 66 67 Bin 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99,100 APG01817.0 68 Cry 92, 93, 94, 95, 96, 97, 98, 99, 100 APG01819.0 6970 Cry 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100APG01860.0 71 72 Cry 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99,100 APG01967.0 73 74 MTX 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99,100 APG02071.0 75 76 Cry 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92,93, 94, 95, 96, 97, 98, 99, 100 APG02105.0 77 MTX 97, 98, 99, 100APG02110.0 78 79 Cry 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100APG02115.0 80 MTX 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100APG02143.0 81 MTX 50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94,95, 96, 97, 98, 99, 100 APG02166.0 82 MTX 45, 50, 55, 60, 65, 70, 75,80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG02206.0 83 84 85MTX 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG02221.0 86 87 Cry 60,65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100APG02256.0 88 89 MTX 99, 100 APG02309.0 90 91, 92 Cry 40, 45, 50, 55,60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100APG02315.0 93 94 MTX 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99,100 APG02319.0 95 MTX 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100APG02380.0 96 97 Vip3 92, 93, 94, 95, 96, 97, 98, 99, 100 APG02451.0 9899 MTX 95, 96, 97, 98, 99, 100 APG02475.0 100 101, 102 MTX 97, 98, 99,100 APG02506.0 103 104 Cry 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95,96, 97, 98, 99, 100 APG02511.0 105 106, 107 MTX 55, 60, 65, 70, 75, 80,85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG02587.0 108 109 Cry91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG02592.0 110 MTX 35, 40, 45,50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99,100 APG02656.0 111 112 Cry 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55,60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100APG02659.0 113 114 MTX 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95,96, 97, 98, 99, 100 APG02671.0 115 116 Cyt 85, 90, 91, 92, 93, 94, 95,96, 97, 98, 99, 100 APG02676.0 117 MTX 5, 10, 15, 20, 25, 30, 35, 40,45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98,99, 100 APG02706.0 118 119, 120, Cry 50, 55, 60, 65, 70, 75, 121, 123,80, 85, 90, 91, 92, 124, 122 93, 94, 95, 96, 97, 98, 99, 100 APG02714.0125 MTX 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100APG02718.0 126 127 MTX 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85,90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG02767.0 128 129 Vip3 90,91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG02941.0 130 MTX 85, 90, 91,92, 93, 94, 95, 96, 97, 98, 99, 100 APG02947.0 131 132, 133 Cry 95, 96,97, 98, 99, 100 APG02956.0 134 MTX 65, 70, 75, 80, 85, 90, 91, 92, 93,94, 95, 96, 97, 98, 99, 100 APG02986.0 135 Cry 55, 60, 65, 70, 75, 80,85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG03018.0 136 137, 138139 Cry 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG03054.0 140Cry 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97,98, 99, 100 APG03056.0 141 142 143 Cry 60, 65, 70, 75, 80, 85, 90, 91,92, 93, 94, 95, 96, 97, 98, 99, 100 APG03182.0 144 145, 146 MTX 65, 70,75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG03218.0 147148 MTX 50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97,98, 99, 100 APG03247.0 149 150 Cry 90, 91, 92, 93, 94, 95, 96, 97, 98,99, 100 APG03361.0 151 Bin 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 91,92, 93, 94, 95, 96, 97, 98, 99, 100 APG03392.0 152 153 Cry 75, 80, 85,90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG03415.0 154 MTX 45, 50,55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100APG03504.0 155 MTX 93, 94, 95, 96, 97, 98, 99, 100 APG03505.0 156 157,159, Cry 75, 80, 85, 90, 91, 92, 160, 158, 93, 94, 95, 96, 97, 161, 16298, 99, 100 APG03527.0 163 164 MTX 90, 91, 92, 93, 94, 95, 96, 97, 98,99, 100 APG03584.0 165 166 MTX 40, 45, 50, 55, 60, 65, 70, 75, 80, 85,90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG03587.0 167 MTX 80, 85,90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG03674.0 168 169 Cyt 96,97, 98, 99, 100 APG03738.0 170 MTX 75, 80, 85, 90, 91, 92, 93, 94, 95,96, 97, 98, 99, 100 APG03748.0 171 172 Bin 92, 93, 94, 95, 96, 97, 98,99, 100 APG03758.0 173 MTX/ 45, 50, 55, 60, 65, 70, PI- 75, 80, 85, 90,91, PLC 92, 93, 94, 95, 96, 97, 98, 99, 100 APG03778.0 174 Bin 50, 55,60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100APG03789.0 175 Bin 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95,96, 97, 98, 99, 100 APG03826.0 176 MTX 30, 35, 40, 45, 50, 55, 60, 65,70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG03887.0177 MTX 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95,96, 97, 98, 99, 100 APG03893.0 178 Cry 90, 91, 92, 93, 94, 95, 96, 97,98, 99, 100 APG03909.0 179 180 Cry 35, 40, 45, 50, 55, 60, 65, 70, 75,80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG03959.0 181 Cyt95, 96, 97, 98, 99, 100 APG04009.0 182 MTX 45, 50, 55, 60, 65, 70, 75,80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG04019.0 183 184Bin 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96,97, 98, 99, 100 APG04103.0 185 186 MTX 70, 75, 80, 85, 90, 91, 92, 93,94, 95, 96, 97, 98, 99, 100 APG04111.0 187 MTX 60, 65, 70, 75, 80, 85,90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG04141.0 188 Cry 95, 96,97, 98, 99, 100 APG04162.0 189 190 MTX 55, 60, 65, 70, 75, 80, 85, 90,91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG04208.0 191 192 Bin 85, 90,91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG04302.0 193 Bin 85, 90, 91,92, 93, 94, 95, 96, 97, 98, 99, 100 APG04316.0 194 195 MTX 80, 85, 90,91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG04323.0 196 MTX 75, 80, 85,90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG04360.0 197 Cyt 95, 96,97, 98, 99, 100 APG04439.0 198 199 MTX 30, 35, 40, 45, 50, 55, 60, 65,70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG04442.0200 201 MTX 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG04476.0202 203 Cry 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG04568.0 204205 Bin 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG04602.0 206 207 Vip455, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100APG04651.0 208 Cry 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93,94, 95, 96, 97, 98, 99, 100 APG04683.0 209 210, 211 MTX 55, 60, 65, 70,75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG04687.0 212213, 214 MTX 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100APG04692.0 215 216, 217 MTX 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90,91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG04694.0 218 219 220 Cry 55,60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100APG04786.0 221 222, 223 Cry 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96,97, 98, 99, 100 APG04794.0 224 MTX 5, 10, 15, 20, 25, 30, 35, 40, 45,50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99,100 APG04821.0 225 226 MTX 99, 100 APG04822.0 227 228 MTX 85, 90, 91,92, 93, 94, 95, 96, 97, 98, 99, 100 APG04959.0 229 MTX 45, 50, 55, 60,65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100APG04966.0 230 231 Bin 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100APG04977.0 232 MTX 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99,100 APG05073.0 233 234, 235 Cry 50, 55, 60, 65, 70, 75, 80, 85, 90, 91,92, 93, 94, 95, 96, 97, 98, 99, 100 APG05078.0 236 Cry34 5, 10, 15, 20,25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94,95, 96, 97, 98, 99, 100 APG05095.0 237 238 Cry 45, 50, 55, 60, 65, 70,75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG05115.0 239MAC 85, 90, 91, 92, 93, 94, PF 95, 96, 97, 98, 99, 100 APG05149.0 240241 MTX 99, 100 APG05279.0 242 243, 244 Cry 35, 40, 45, 50, 55, 60, 65,70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG05300.0245 MTX 93, 94, 95, 96, 97, 98, 99, 100 APG05346.0 246 247 MTX 93, 94,95, 96, 97, 98, 99, 100 APG05388.0 248 MTX 91, 92, 93, 94, 95, 96, 97,98, 99, 100 APG05408.0 249 250 Cry34 40, 45, 50, 55, 60, 65, 70, 75, 80,85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG05414.0 251 252 MTX75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG05429.0 253254, 255 MTX 92, 93, 94, 95, 96, 97, 98, 99, 100 APG05431.0 256 257 MTX85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG05471.0 258 MAC 30,35, 40, 45, 50, 55, PF 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95,96, 97, 98, 99, 100 APG05481.0 259 260, 261 MTX 90, 91, 92, 93, 94, 95,96, 97, 98, 99, 100 APG05492.0 262 MTX 90, 91, 92, 93, 94, 95, 96, 97,98, 99, 100 APG05508.0 263 264 Cry 80, 85, 90, 91, 92, 93, 94, 95, 96,97, 98, 99, 100 APG05538.0 265 266, 267 Cry 70, 75, 80, 85, 90, 91, 92,93, 94, 95, 96, 97, 98, 99, 100 APG05636.0 268 MTX 98, 99, 100APG05643.0 269 270 271 Cry 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95,96, 97, 98, 99, 100 APG05662.0 272 Cyt 93, 94, 95, 96, 97, 98, 99, 100APG05725.0 273 274 Cry 50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93,94, 95, 96, 97, 98, 99, 100 APG05734.0 275 276, 277, Cry 90, 91, 92, 93,94, 95, 278, 279 96, 97, 98, 99, 100 APG05793.0 280 MTX 65, 70, 75, 80,85, 90, 91, 92, 93, 94, 95 96, 97, 98, 99, 100 APG05834.0 281 282 MTX65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100APG05878.0 283 Cry 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98,99, 100 APG05907.0 284 285 Bin 85, 90, 91, 92, 93, 94, 95, 96, 97, 98,99, 100 APG05917.0 286 Bin 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100APG05920.0 287 288 Cry 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85,90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG05946.0 289 Bin 65, 70,75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG05955.0 290Bin 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96,97, 98, 99, 100 APG05992.0 291 MTX 85, 90, 91, 92, 93, 94, 95, 96, 97,98, 99, 100 APG06027.0 292 293 Bin 85, 90, 91, 92, 93, 94, 95, 96, 97,98, 99, 100 APG06049.0 294 295 MTX 85, 90, 91, 92, 93, 94, 95, 96, 97,98, 99, 100 APG06079.0 296 297 Cry 91, 92, 93, 94, 95, 96, 97, 98, 99,100 APG06195.0 298 MTX 97, 98, 99, 100 APG06213.0 299 300, 301 Cry 85,90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG06235.0 302 Vip3 93, 94,95, 96, 97, 98, 99, 100 APG06283.0 303 MTX 70, 75, 80, 85, 90, 91, 92,93, 94, 95, 96, 97, 98, 99, 100 APG06304.0 304 305 MTX 55, 60, 65, 70,75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG06319.0 306307 MTX 92, 93, 94, 95, 96, 97, 98, 99, 100 APG06331.0 308 309 Bin 94,95, 96, 97, 98, 99, 100 APG06350.0 310 311 MTX 5, 10, 15, 20, 25, 30,35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96,97, 98, 99, 100 APG06378.0 312 Vip 70, 75, 80, 85, 90, 91, 92, 93, 94,95, 96, 97, 98, 99, 100 APG06424.0 313 Cry 60, 65, 70, 75, 80, 85, 90,91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG06479.0 314 315 MTX 98, 99,100 APG06480.0 316 317 MTX 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99,100 APG06493.0 318 319 Cry 95, 96, 97, 98, 99, 100 APG06496.0 320 Cyt45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98,99, 100 APG06559.0 321 Bin 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100APG06576.0 322 323 Cry 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98,99, 100 APG06578.0 324 MTX 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90,91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG06606.0 325 326, 327 Cry 85,90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG06677.0 328 329 Cry 75,80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG06697.0 330 331,332 Bin 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG06705.0333 334, 335 Cry 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG06718.0336 Cyt 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93,94, 95, 96, 97, 98, 99, 100 APG06738.0 337 338 MTX 55, 60, 65, 70, 75,80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG06774.0 339 Cry40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97,98, 99, 100 APG06791.0 340 341 MTX 60, 65, 70, 75, 80, 85, 90, 91, 92,93, 94, 95, 96, 97, 98, 99, 100 APG06883.0 342 343 Cry 65, 70, 75, 80,85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG06898.0 344 345 Bin80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG06937.0 346 347Cyt 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG06954.0 348 349MTX 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85,90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG07054.0 350 Vip3 55, 60,65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100APG07057.0 351 Cry 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95,96, 97, 98, 99, 100 APG07094.0 352 353 MTX 96, 97, 98, 99, 100APG07107.0 354 355 MTX 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98,99, 100 APG07137.0 356 357, 358 MTX 75, 80, 85, 90, 91, 92, 93, 94, 95,96, 97, 98, 99, 100 APG07162.0 359 Cry 98, 99, 100 APG07176.0 360 MTX35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96,97, 98, 99, 100 APG07249.0 361 362, 363 MTX 45, 50, 55, 60, 65, 70, 75,80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG07257.0 364 365Bin 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96,97, 98, 99, 100 APG07307.0 366 367 Cry34 5, 10, 15, 20, 25, 30, 35, 40,45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98,99, 100 APG07310.0 368 Bin 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 91,92, 93, 94, 95, 96, 97, 98, 99, 100 APG07318.0 369 370 Vip4 75, 80, 85,90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG07322.0 371 372, 373 374Cry 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG07432.0 375376 Bin 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG07463.0377 MTX 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94,95, 96, 97, 98, 99, 100 APG07467.0 378 379 MTX 96, 97, 98, 99, 100APG07487.0 380 MTX 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100APG07511.0 381 382 Bin 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100APG07564.0 383 384 Cry 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100APG07609.0 385 386 MTX 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92,93, 94, 95, 96, 97, 98, 99, 100 APG07636.0 387 388 Cry 65, 70, 75, 80,85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG07664.0 389 390 Cry3450, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99,100 APG07665.0 391 392 Cry 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98,99, 100 APG07732.0 393 MTX 50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92,93, 94, 95, 96, 97, 98, 99, 100 APG07753.0 394 395 MTX 55, 60, 65, 70,75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG07798.0 396397 Cry 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80,85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG07810.0 398 Cry 45,50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99,100 APG07811.0 399 400, 401 Cry 85, 90, 91, 92, 93, 94, 95, 96, 97, 98,99, 100 APG07945.0 402 403 404 Cry 65, 70, 75, 80, 85, 90, 91, 92, 93,94, 95, 96, 97, 98, 99, 100 APG07969.0 405 406 MTX 40, 45, 50, 55, 60,65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100APG08004.0 407 408 Bin 96, 97, 98, 99, 100 APG08021.0 409 410 Cry 70,75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG08081.0 411412, 413 PI- 55, 60, 65, 70, 75, 80, PLC 85, 90, 91, 92, 93, 94, 95, 96,97, 98, 99, 100 APG08171.0 414 Cry 65, 70, 75, 80, 85, 90, 91, 92, 93,94, 95, 96, 97, 98, 99, 100 APG08193.0 415 416 Bin 70, 75, 80, 85, 90,91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG08207.0 417 418, 419 MTX 60,65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100APG08240.0 420 MTX 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100APG08316.0 421 MTX 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65,70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG08335.0422 423 MTX 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92,93, 94, 95, 96, 97, 98, 99, 100 APG08339.0 424 425 MTX 94, 95, 96, 97,98, 99, 100 APG08370.0 426 427 MAC 5, 10, 15, 20, 25, 30, PF 35, 40, 45,50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99,100 APG08378.0 428 429 430 Cry 80, 85, 90, 91, 92, 93, 94, 95, 96, 97,98, 99, 100 APG08427.0 431 432 Bin 50, 55, 60, 65, 70, 75, 80, 85, 90,91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG08468.0 433 Cry 60, 65, 70,75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG08495.0 434MTX 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96,97, 98, 99, 100 APG08526.0 435 Cry 80, 85, 90, 91, 92, 93, 94, 95, 96,97, 98, 99, 100 APG08576.0 436 MTX 90, 91, 92, 93, 94, 95, 96, 97, 98,99, 100 APG08577.0 437 MTX 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 91,92, 93, 94, 95, 96, 97, 98, 99, 100 APG08624.0 438 439 Bin 55, 60, 65,70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG08625.0440 441 MTX 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG08639.0 442MTX 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100APG08642.0 443 444, 445 Cry 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99,100 APG08757.0 446 447 MTX 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99,100 APG08765.0 448 449 Cry 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90,91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG08791.0 450 451, 452 MTX 94,95, 96, 97, 98, 99, 100 APG08812.0 453 MTX 35, 40, 45, 50, 55, 60, 65,70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG08823.0454 MTX 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100APG08852.0 455 Cry 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96,97, 98, 99, 100 APG08912.0 456 457, 458 MTX 90, 91, 92, 93, 94, 95, 96,97, 98, 99, 100 APG09027.0 459 460 Bin 90, 91, 92, 93, 94, 95, 96, 97,98, 99, 100 APG09028.0 461 462 Cry 50, 55, 60, 65, 70, 75, 80, 85, 90,91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG09048.0 463 464 MTX 90, 91,92, 93, 94, 95, 96, 97, 98, 99, 100 APG09152.0 465 466 MTX 80, 85, 90,91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG09158.0 467 468 MTX 45, 50,55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100APG09181.0 469 Cyt 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 91,92, 93, 94, 95, 96, 97, 98, 99, 100 APG09201.0 470 Cyt 5, 10, 15, 20,25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94,95, 96, 97, 98, 99, 100 APG09210.0 471 Cry 55, 60, 65, 70, 75, 80, 85,90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG09221.0 472 Vip3 97, 98,99, 100 APG09238.0 473 474, 475 Vip4 98, 99, 100 APG09266.0 476 477 Bin85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG09289.0 478 479, 480Cry 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95,96, 97, 98, 99, 100 APG09323.0 481 482 Bin 93, 94, 95, 96, 97, 98, 99,100 APG09412.0 483 484 MTX 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95,96, 97, 98, 99, 100 APG09415.0 485 486 MTX 75, 80, 85, 90, 91, 92, 93,94, 95, 96, 97, 98, 99, 100 APG09416.0 487 MTX 93, 94, 95, 96, 97, 98,99, 100 APG09430.0 488 489 Cry 90, 91, 92, 93, 94, 95, 96, 97, 98, 99,100 APG09439.0 490 MTX 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99,100 APG09465.0 491 492 Bin 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100APG09511.0 493 494 Cry 99, 100 APG09548.0 495 496 497 Cry 45, 50, 55,60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100APG09675.0 498 MTX 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97,98, 99, 100 APG09773.0 499 Cry 70, 75, 80, 85, 90, 91, 92, 93, 94, 95,96, 97, 98, 99, 100 APG09812.0 500 Cry 97, 98, 99, 100 APG09814.0 501Cry 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG09826.0 502503 Cry 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG09835.0 504Cry 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100APG09895.0 505 506 Cry 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96,97, 98, 99, 100 APG09901.0 507 508, 509 MTX 90, 91, 92, 93, 94, 95, 96,97, 98, 99, 100 APG09903.0 510 511 Cry 30, 35, 40, 45, 50, 55, 60, 65,70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG10001.0512 513, 514 MTX 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG01902.0 515516 Cry 98, 99, 100 APG02996.0 517 518 Cry 97, 98, 99, 100 APG03098.0519 520 Cry 98, 99, 100 APG04517.0 521 Cry 92, 93, 94, 95, 96, 97, 98,99, 100 APG04619.0 522 523 Cry 98, 99, 100 APG06717.0 524 525 Cry 97,98, 99, 100 APG ID % Similarity Homologs APG00751.0 70, 75, 80, 85, 90,91, APG08884.0 (54.39% identity, 68.5% 92, 93, 94, 95, 96, similarity)97, 98, 99, 100 APG02841.0 (44.17% identity, 58.16% similarity)APG00162.0 US_2016_0177333_A1-82 (43.57% identity, 59.16% similarity)WP_098826687.1 (43.57% identity, 59.16% similarity) APG03056.0 (42.69%identity, 58.27% similarity) APG00374.0 US_2016_0366881_A1-40 (40.36%identity, 55.78% similarity) US_6056953_A-28 (38.67% identity, 54.85%similarity) ASO64551.1 (38.43% identity, 54.51% similarity)CA_2516349-38 (38.39% identity, 54.6% similarity) AND28677.1 (38.39%identity, 54.45% similarity) APG00831.0 70, 75, 80, 85, 90, 91,CA_2844913-48 (58.85% identity, 60.27% 92, 93, 94, 95, 96, similarity)97, 98, 99, 100 APG06942.0 (58.22% identity, 60.19% similarity)APG00688.0 US_2017_0175134_A1-104 (49.52% identity, 66.55% similarity)WP_098088433.1 (47.28% identity, 63.15% similarity) WP_098098942.1(47.19% identity, 63.46% similarity) US_9695440_B2-14 (41.35% identity,52.76% similarity) APG00103.0 US_2016_0304898_A1-132 (41.31% identity,55.71% similarity) US_9695440_B2-13 (41.24% identity, 52.56% similarity)US_8461421_B2-168 (40.9% identity, 52.56% similarity) US_8759619_B2-28(40.59% identity, 54.47% similarity) APG00832.0 60, 65, 70, 75, 80, 85,APG01634.0 (40.14% identity, 55.49% 90, 91, 92, 93, 94, similarity) 95,96, 97, 98, 99, WP_016099738.1 (39.15% identity, 56.6% 100 similarity)US_8318900_B2-111 (37.05% identity, 51.93% similarity) WP_103574454.1(36.88% identity, 53.62% similarity) US_8318900_B2-80 (36.8% identity,51.57% similarity) US_8461415_B2-28 (36.31% identity, 52.79% similarity)APG09227.0 (36.08% identity, 53.01% similarity) APG07799.0 (35.47%identity, 51.98% similarity) US_8461415_B2-1 (35.38% identity, 51.61%similarity) APG00864.0 US_2017_0175134_A1-174 (31.74% identity, 44.13%similarity) APG00842.0 5, 10, 15, 20, 25, 30, WP_004801413.1 (94.08%identity, 94.08% 35, 40, 45, 50, 55, similarity) 60, 65, 70, 75, 80, 85,WP_033133379.1 (94.08% identity, 94.08% 90, 91, 92, 93, 94, similarity)95, 96, 97, 98, 99, WP_005202252.1 (93.75% identity, 94.08% 100similarity) WP_005322969.1 (93.42% identity, 94.08% similarity)WP_016162910.1 (93.42% identity, 93.75% similarity) WP_063645785.1(65.13% identity, 65.79% similarity) WP_090386676.1 (28.23% identity,44.74% similarity) WP_018616622.1 (26.99% identity, 43.56% similarity)PCJ91616.1 (25.31% identity, 45.0% similarity) APG00887.0 90, 91, 92,93, 94, 95, APG02970.0 (82.08% identity, 87.57% 96, 97, 98, 99, 100similarity) APG02808.0 (81.79% identity, 87.57% similarity) APG05239.0(80.64% identity, 89.02% similarity) APG01367.0 (80.35% identity, 86.99%similarity) APG05140.0 (80.06% identity, 86.71% similarity) APG05040.0(78.32% identity, 86.13% similarity) APG00793.0 US_2016_0355842_A1-170(46.31% identity, 64.2% similarity) APG07753.0 (41.11% identity, 55.28%similarity) APG09460.0 (41.05% identity, 54.82% similarity) APG00416.0US_2016_0355842_A1-82 (40.22% identity, 53.72% similarity)WP_098425282.1 (40.22% identity, 53.72% similarity) APG00961.0 90, 91,92, 93, 94, 95, APG00084.0 US_2016_0311864_A1-15 96, 97, 98, 99, 100(82.22% identity, 87.03% similarity) APG06921.0 (61.73% identity, 73.75%similarity) APG00152.0 US_2016_0311864_A1-36 (57.82% identity, 68.87%similarity) WP_098381669.1 (56.94% identity, 69.1% similarity)APG07954.0 (56.78% identity, 69.04% similarity) WP_098377949.1 (56.78%identity, 69.04% similarity) APG00164.0 US_2016_0311864_A1-41 (55.11%identity, 69.37% similarity) EEM92570.1 (55.06% identity, 67.86%similarity) CA_2844913-12 (54.1% identity, 67.34% similarity)WP_098209375.1 (54.1% identity, 67.34% similarity) APG00973.0 50, 55,60, 65, 70, 75, ANN35812.1 (45.86% identity, 59.34% 80, 85, 90, 91, 92,similarity) 93, 94, 95, 96, 97, APG06236.0 (36.64% identity, 48.76% 98,99, 100 similarity) APG04375.0 (35.14% identity, 46.76% similarity)APG03686.0 (34.79% identity, 46.58% similarity) APG04793.0 (34.52%identity, 46.3% similarity) WP_098162508.1 (34.52% identity, 46.3%similarity) WP_098908549.1 (34.52% identity, 46.3% similarity)APG05300.0 (34.44% identity, 46.83% similarity) APG07676.0 (34.25%identity, 46.03% similarity) APG08525.0 (34.25% identity, 45.75%similarity) APG01038.0 80, 85, 90, 91, 92, 93, APG09572.0 (61.83%identity, 74.76% 94, 95, 96, 97, 98, similarity) 99, 100 APG03867.0(61.61% identity, 74.61% similarity) APG00143.0 US_2016_0355842_A1-4(60.44% identity, 75.39% similarity) APG00441.0 US_2016_0355842_A1-91(60.0% identity, 74.69% similarity) APG00833.0 US_2016_0355842_A1-175(59.81% identity, 75.7% similarity) WP_098858716.1 (59.81% identity,75.7% similarity) APG00444.0 US_2016_0355842_A1-93 (59.69% identity,75.0% similarity) APG03227.0 (58.07% identity, 73.29% similarity)APG04037.0 (57.72% identity, 70.99% similarity) APG07682.0 (57.4%identity, 69.49% similarity) WP_098268663.1 (57.4% identity, 69.49%similarity) WP_098768822.1 (57.4% identity, 69.49% similarity)APG01063.0 80, 85, 90, 91, 92, 93, APG04208.0 (71.64% identity, 81.17%94, 95, 96, 97, 98, similarity) 99, 100 WP_098412831.1 (71.32% identity,81.13% similarity) APG00295.0 US_2016_0311864_A1-128 (70.99% identity,79.25% similarity) APG00308.0 US_2016_0311864_A1-135 (70.32% identity,78.1% similarity) WP_098386694.1 (65.38% identity, 75.54% similarity)WP_098858722.1 (64.75% identity, 74.34% similarity) APG00369.0US_2016_0311864_A1-149 (64.67% identity, 73.56% similarity) APG00419.0US_2016_0311864_A1-176 (63.99% identity, 75.43% similarity)WP_098634587.1 (63.99% identity, 75.43% similarity) WP_098205870.1(63.88% identity, 75.43% similarity) APG01092.0 75, 80, 85, 90, 91, 92,APG01686.0 (75.19% identity, 82.77% 93, 94, 95, 96, 97, similarity) 98,99, 100 APG04682.0 (56.32% identity, 70.7% similarity) APG02630.0 (56.0%identity, 70.61% similarity) US_8759619_B2-17 (34.43% identity, 49.34%similarity) US_8759619_B2-3 (34.43% identity, 49.34% similarity)CA_2595901-4 (33.75% identity, 49.63% similarity) US_8759619_B2-15(33.02% identity, 50.84% similarity) APG00070.0 US_2016_0177333_A1-37(32.39% identity, 45.82% similarity) APG02391.0 (32.02% identity, 46.14%similarity) US_8759619_B2-13 (31.85% identity, 45.26% similarity)APG01102.0 80, 85, 90, 91, 92, 93, APG00383.0 US_2017_0175134_A1-6 94,95, 96, 97, 98, (66.21% identity, 75.71% similarity) 99, 100 APG00101.0US_2016_0177333_A1-57 (64.86% identity, 74.35% similarity)WP_098976575.1 (64.86% identity, 74.35% similarity) APG05886.0 (56.42%identity, 69.71% similarity) OPA06342.1 (55.47% identity, 67.98%similarity) WP_078401734.1 (55.1% identity, 67.53% similarity)APG03555.0 (54.34% identity, 67.15% similarity) APG00048.0US_2016_0177333_A1-22 (54.24% identity, 64.72% similarity)WP_098139461.1 (54.24% identity, 64.72% similarity) APG08578.0 (53.9%identity, 67.26% similarity) APG01127.0 98, 99, 100 APG07655.0 (94.61%identity, 97.31% similarity) APG00155.0 US_2016_0311864_A1-40 (92.93%identity, 96.97% similarity) APG03504.0 (92.93% identity, 96.97%similarity) APG03379.0 (82.94% identity, 89.3% similarity) APG00749.0(81.4% identity, 87.38% similarity) APG01506.0 (80.47% identity, 87.54%similarity) APG09682.0 (80.27% identity, 87.63% similarity) APG09416.0(80.0% identity, 86.33% similarity) APG08241.0 (79.8% identity, 86.2%similarity) APG02638.0 (79.33% identity, 87.67% similarity) APG01141.090, 91, 92, 93, 94, 95, APG00591.0 US_2016_0311864_A1-194 96, 97, 98,99, 100 (84.36% identity, 88.55% similarity) WP_098290048.1 (84.36%identity, 88.55% similarity) APG00844.0 US_2016_0311864_A1-221 (47.31%identity, 59.85% similarity) APG01059.0 (46.8% identity, 59.34%similarity) APG01810.0 (46.62% identity, 59.4% similarity) APG00353.0US_2016_0311864_A1-144 (46.43% identity, 59.44% similarity)WP_099003921.1 (46.43% identity, 59.44% similarity) APG00203.0US_2016_0311864_A1-69 (46.43% identity, 58.67% similarity)WP_098475832.1 (46.43% identity, 58.67% similarity) WP_033679178.1(46.19% identity, 59.14% similarity) APG01163.0 65, 70, 75, 80, 85, 90,APG07249.0 (60.92% identity, 73.85% 91, 92, 93, 94, 95, similarity) 96,97, 98, 99, 100 APG00580.0 US_2016_0355842_A1-132 (45.61% identity,61.4% similarity) WP_098146720.1 (45.61% identity, 61.4% similarity)APG00594.0 US_2016_0355842_A1-137 (42.11% identity, 54.02% similarity)WP_097795410.1 (42.11% identity, 54.02% similarity) APG00154.0US_2016_0355842_A1-5 (42.11% identity, 53.74% similarity)US_8461415_B2-38 (41.92% identity, 55.07% similarity) APG00414.0US_2016_0355842_A1-81 (41.53% identity, 57.67% similarity) APG00995.0US_2016_0355842_A1-200 (41.53% identity, 57.14% similarity) APG00593.0US_2016_0355842_A1-136 (41.53% identity, 56.88% similarity)WP_097899326.1 (41.53% identity, 56.88% similarity) APG01165.0 80, 85,90, 91, 92, 93, APG09773.0 (95.13% identity, 96.43% 94, 95, 96, 97, 98,similarity) 99, 100 APG00296.0 US_2016_0355842_A1-52 (67.09% identity,77.95% similarity) WP_098926211.1 (67.09% identity, 77.95% similarity)APG07033.0 (65.78% identity, 76.3% similarity) APG03264.0 (57.83%identity, 71.57% similarity) APG06221.0 (52.83% identity, 70.11%similarity) APG00553.0 US_2016_0355842_A1-124 (51.05% identity, 69.31%similarity) WP_098926212.1 (51.05% identity, 69.31% similarity)APG04454.0 (50.72% identity, 68.26% similarity) APG09727.0 (50.57%identity, 68.29% similarity) APG01201.0 70, 75, 80, 85, 90, 91,APG03218.0 (52.54% identity, 68.08% 92, 93, 94, 95, 96, similarity) 97,98, 99, 100 APG00479.0 US_2016_0355842_A1-101 (47.75% identity, 63.48%similarity) WP_098139684.1 (47.75% identity, 63.48% similarity)WP_098139851.1 (47.58% identity, 67.52% similarity) APG00966.0US_2016_0355842_A1-195 (47.29% identity, 63.82% similarity)WP_098976737.1 (47.29% identity, 63.82% similarity) APG00283.0US_2016_0355842_A1-50 (46.52% identity, 66.02% similarity) APG03623.0(40.88% identity, 57.18% similarity) APG00411.0 US_2016_0355842_A1-80(39.89% identity, 55.59% similarity) WP_065486138.1 (37.82% identity,59.66% similarity) WP_065487080.1 (37.82% identity, 59.66% similarity)APG01281.0 75, 80, 85, 90, 91, 92, APG05341.0 (64.8% identity, 71.68%93, 94, 95, 96, 97, similarity) 98, 99, 100 APG00324.0US_2016_0355842_A1-61 (63.96% identity, 74.37% similarity) APG05481.0(61.44% identity, 70.69% similarity) APG00253.0 US_2016_0355842_A1-39(44.83% identity, 60.48% similarity) APG04342.0 (44.56% identity, 62.86%similarity) APG00020.0 US_2016_0304898_A1-33 (44.56% identity, 60.48%similarity) WP_098539891.1 (44.56% identity, 60.48% similarity)WP_098178928.1 (44.56% identity, 59.68% similarity) WP_098908888.1(44.56% identity, 59.42% similarity) APG01508.0 (44.3% identity, 59.95%similarity) WP_098322759.1 (44.3% identity, 59.95% similarity)APG01352.0 65, 70, 75, 80, 85, 90, APG00729.0 US_2017_0175134_A1-142 91,92, 93, 94, 95, (45.1% identity, 61.76% similarity) 96, 97, 98, 99, 100APG00880.0 US_2016_0366881_A1-134 (34.5% identity, 49.0% similarity)APG00215.0 US_2016_0311864_A1-78 (34.16% identity, 51.49% similarity)APG00837.0 (33.5% identity, 50.37% similarity) WP_002167240.1 (32.68%identity, 48.54% similarity) APG04536.0 (32.67% identity, 48.38%similarity) WP_016093722.1 (32.67% identity, 48.38% similarity)APG05475.0 (32.33% identity, 48.62% similarity) WP_048517129.1 (32.24%identity, 50.88% similarity) APG08193.0 (32.18% identity, 50.74%similarity) APG01380.0 5, 10, 15, 20, 25, 30, APG05078.0 (96.52%identity, 98.26% 35, 40, 45, 50, 55, similarity) 60, 65, 70, 75, 80, 85,WP_077106733.1 (91.3% identity, 93.91% 90, 91, 92, 93, 94, similarity)95, 96, 97, 98, 99, WP_103583963.1 (91.3% identity, 93.91% 100similarity) APG01385.0 98, 99, 100 APG00641.0 US_2017_0175134_A1-66(96.69% identity, 97.68% similarity) APG00807.0 US_2017_0175134_A1-169(75.08% identity, 83.06% similarity) APG00434.0 US_2016_0366881_A1-58(50.48% identity, 67.52% similarity) US_9567381_B2-446 (32.49% identity,47.95% similarity) APG00866.0 US_2016_0355842_A1-183 (32.32% identity,50.3% similarity) WP_098072344.1 (32.32% identity, 50.3% similarity)APG02068.0 (32.22% identity, 47.11% similarity) APG00112.0US_2016_0311864_A1-23 (31.83% identity, 46.85% similarity)WP_098162515.1 (31.83% identity, 46.85% similarity) APG00360.0US_2016_0355842_A1-69 (31.78% identity, 47.98% similarity) APG01396.080, 85, 90, 91, 92, 93, US_8829279_B2-42 (69.11% identity, 94, 95, 96,97, 98, 79.2% similarity) 99, 100 WP_098228105.1 (32.56% identity,52.62% similarity) APG00218.0 US_2016_0355842_A1-26 (32.28% identity,52.16% similarity) APG00245.0 US_2016_0355842_A1-34 (32.08% identity,52.02% similarity) APG03900.0 (31.01% identity, 50.28% similarity)APG01189.0 (30.95% identity, 46.42% similarity) APG00187.0US_2016_0355842_A1-20 (29.23% identity, 46.13% similarity)WP_098228102.1 (29.23% identity, 46.13% similarity) APG01434.0 90, 91,92, 93, 94, 95, AGV55019.1 (81.33% identity, 87.65% 96, 97, 98, 99, 100similarity) APG00986.0 US_2017_0175134_A1-190 (80.54% identity, 87.48%similarity) WP_098223384.1 (65.63% identity, 77.04% similarity)CA_2658677-2 (64.37% identity, 75.95% similarity) APG00518.0US_2016_0366881_A1-104 (58.32% identity, 60.57% similarity)WP_103592286.1 (58.04% identity, 72.17% similarity) APG03018.0 (56.48%identity, 71.09% similarity) WP_085313394.1 (56.18% identity, 70.79%similarity) WP_050845711.1 (55.34% identity, 69.29% similarity)US_9567381_B2-427 (55.03% identity, 68.93% similarity) APG01455.0 98,99, 100 US_7214788_B2-4 (96.83% identity, 97.62% similarity)US_9328356_B2-34 (92.06% identity, 96.03% similarity) CA_2267665-4(80.16% identity, 88.1% similarity) AHA43421.1 (80.16% identity, 87.3%similarity) US_9328356_B2-6 (66.67% identity, 73.33% similarity)US_7214788_B2-10 (30.81% identity, 31.06% similarity) US_7214788_B2-6(30.81% identity, 31.06% similarity) US_7214788_B2-14 (30.58% identity,30.83% similarity) US_7214788_B2-33 (30.5% identity, 30.75% similarity)US_7214788_B2-8 (30.5% identity, 30.75% similarity) APG01516.0 85, 90,91, 92, 93, 94, APG04687.0 (72.39% identity, 84.45% 95, 96, 97, 98, 99,similarity) 100 APG06242.0 (72.3% identity, 83.91% similarity)US_8829279_B2-11 (61.85% identity, 75.75% similarity) APG09338.0 (57.07%identity, 67.74% similarity) APG06273.0 (56.39% identity, 70.28%similarity) APG00051.0 US_2016_0304898_A1-81 (55.89% identity, 70.93%similarity) WP_098425241.1 (55.89% identity, 70.93% similarity)APG01858.0 (55.47% identity, 67.66% similarity) WP_098229435.1 (54.1%identity, 65.3% similarity) APG07055.0 (54.0% identity, 64.16%similarity) APG01549.0 70, 75, 80, 85, 90, 91, US_8318900_B2-96 (51.1%identity, 92, 93, 94, 95, 96, 67.82% similarity) 97, 98, 99, 100WP_044797748.1 (48.09% identity, 64.65% similarity) APG03053.0 (44.41%identity, 62.84% similarity) APG00098.0 US_2016_0304898_A1-128 (44.19%identity, 60.34% similarity) APG00899.0 US_2016_0355842_A1-188 (44.19%identity, 60.34% similarity) WP_098162516.1 (44.19% identity, 60.34%similarity) WP_098908554.1 (44.19% identity, 60.34% similarity)APG01885.0 (44.13% identity, 60.63% similarity) US_9328356_B2-46 (32.84%identity, 48.36% similarity) US_9567381_B2-441 (32.84% identity, 48.06%similarity) APG01617.0 90, 91, 92, 93, 94, 95, CA_2844913-54 (81.85%identity, 88.69% 96, 97, 98, 99, 100 similarity) APG00509.0US_2016_0355842_A1-113 (35.03% identity, 46.33% similarity) APG00541.0US_2016_0355842_A1-119 (34.46% identity, 46.05% similarity)WP_097855808.1 (34.46% identity, 46.05% similarity) WP_098581475.1(34.18% identity, 45.76% similarity) CA_2844913-102 (33.05% identity,47.29% similarity) APG06228.0 (32.96% identity, 47.21% similarity)APG00387.0 US_2016_0366881_A1-45 (32.78% identity, 45.0% similarity)APG09155.0 (32.77% identity, 46.89% similarity) APG04477.0 (32.71%identity, 45.74% similarity) APG01681.0 55, 60, 65, 70, 75, 80,US_9321814_B2-2 (38.46% identity, 85, 90, 91, 92, 93, 52.04% similarity)94, 95, 96, 97, 98, US_9321814_B2-3 (38.46% identity, 99, 100 52.04%similarity) WP_098876539.1 (38.46% identity, 52.04% similarity)APG06920.0 (37.92% identity, 51.47% similarity) APG09069.0 (37.64%identity, 52.38% similarity) APG07930.0 (37.56% identity, 52.76%similarity) US_9321814_B2-4 (37.56% identity, 50.9% similarity)APG00413.0 US_2016_0311864_A1-174 (37.44% identity, 52.51% similarity)APG07511.0 (37.41% identity, 52.61% similarity) APG03859.0 (37.35%identity, 52.67% similarity) APG01686.0 70, 75, 80, 85, 90, 91,APG01092.0 (75.19% identity, 82.77% 92, 93, 94, 95, 96, similarity) 97,98, 99, 100 APG04682.0 (54.27% identity, 69.18% similarity) APG02630.0(53.67% identity, 67.84% similarity) US_8759619_B2-17 (34.23% identity,47.88% similarity) US_8759619_B2-3 (34.23% identity, 47.88% similarity)CA_2595901-4 (32.67% identity, 49.33% similarity) APG00070.0US_2016_0177333_A1-37 (32.1% identity, 45.86% similarity) CA_2595901-2(31.82% identity, 48.81% similarity) APG02391.0 (31.57% identity, 45.74%similarity) US_8759619_B2-13 (31.49% identity, 44.89% similarity)APG01710.0 90, 91, 92, 93, 94, 95, EJQ96305.1 (86.63% identity, 91.64%96, 97, 98, 99, 100 similarity) WP_085962534.1 (86.15% identity, 91.14%similarity) APG00616.0 US_2016_0366881_A1-120 (81.22% identity, 88.95%similarity) US_8461421_B2-112 (38.93% identity, 55.74% similarity)AFI42041.1 (38.11% identity, 55.58% similarity) APG00618.0US_2016_0366881_A1-123 (37.92% identity, 53.98% similarity) AEX63666.1(37.8% identity, 53.32% similarity) APG05865.0 (30.44% identity, 44.83%similarity) WP_033728958.1 (29.55% identity, 46.11% similarity)ETT84679.1 (29.17% identity, 45.48% similarity) APG01788.0 55, 60, 65,70, 75, 80, APG00473.0 US_2016_0366881_A1-87 85, 90, 91, 92, 93, (35.45%identity, 53.55% similarity) 94, 95, 96, 97, 98, APG07564.0 (29.99%identity, 44.3% 99, 100 similarity) APG02706.0 (29.57% identity, 43.57%similarity) APG02706.0 (29.57% identity, 43.57% similarity)WP_103655243.1 (28.49% identity, 45.08% similarity)US_2011_0239334_A1-80 (28.49% identity, 44.76% similarity)US_2017_0347664_A1-215 (28.41% identity, 44.68% similarity)US_5670365_A-9 (28.41% identity, 44.68% similarity) US_9567381_B2-1403(28.41% identity, 44.68% similarity) US_7923602_B2-35 (27.98% identity,42.38% similarity) APG01803.0 55, 60, 65, 70, 75, 80, APG00564.0US_2016_0355842_A1-128 85, 90, 91, 92, 93, (34.04% identity, 50.57%similarity) 94, 95, 96, 97, 98, APG00239.0 US_2016_0311864_A1-91 99, 100(31.57% identity, 45.19% similarity) APG04152.0 (30.08% identity, 44.96%similarity) APG06420.0 (29.61% identity, 42.3% similarity) APG00395.0US_2016_0311864_A1-162 (29.57% identity, 43.81% similarity)WP_098397014.1 (29.57% identity, 43.81% similarity) APG02506.0 (27.7%identity, 40.94% similarity) APG07866.0 (27.45% identity, 41.78%similarity) APG05920.0 (27.44% identity, 38.72% similarity) APG00285.0US_2016_0311864_A1-118 (27.4% identity, 39.67% similarity) APG01810.085, 90, 91, 92, 93, 94, APG00301.0 US_2016_0311864_A1-131 95, 96, 97,98, 99, (70.59% identity, 83.12% similarity) 100 WP_033679178.1 (70.44%identity, 83.55% similarity) APG01059.0 (70.23% identity, 82.7%similarity) APG00353.0 US_2016_0311864_A1-144 (70.08% identity, 83.38%similarity) WP_099003921.1 (70.08% identity, 83.38% similarity)APG00203.0 US_2016_0311864_A1-69 (69.82% identity, 83.12% similarity)WP_098475832.1 (69.82% identity, 83.12% similarity) APG00844.0US_2016_0311864_A1-221 (69.21% identity, 81.93% similarity) APG00419.0US_2016_0311864_A1-176 (69.14% identity, 80.99% similarity)WP_098634587.1 (69.14% identity, 80.99% similarity) WP_098205870.1(69.14% identity, 80.74% similarity) APG01817.0 97, 98, 99, 100EP_2728007-45 (91.95% identity, 96.06% similarity) AHB36886.1 (91.61%identity, 95.89% similarity) WP_080989246.1 (88.36% identity, 92.55%similarity) US_9567381_B2-470 (76.22% identity, 84.77% similarity)AEA92303.1 (76.13% identity, 84.69% similarity) AFI42036.1 (71.17%identity, 79.04% similarity) EP_2728007-55 (54.03% identity, 68.15%similarity) US_8829279_B2-21 (53.99% identity, 69.13% similarity)US_8829279_B2-23 (53.94% identity, 69.3% similarity) WP_088065221.1(53.83% identity, 69.05% similarity) APG00960.0 US_2017_0175134_A1-179(53.83% identity, 68.83% similarity) APG01819.0 75, 80, 85, 90, 91, 92,APG02667.0 (69.05% identity, 70.51% 93, 94, 95, 96, 97, similarity) 98,99, 100 APG00787.0 US_2017_0175134_A1-156 (28.77% identity, 42.92%similarity) APG03986.0 (27.36% identity, 43.87% similarity) APG00564.0US_2016_0355842_A1-128 (27.32% identity, 43.61% similarity) APG00702.0US_2017_0175134_A1-115 (27.29% identity, 43.6% similarity) APG05538.0(26.96% identity, 40.21% similarity) APG09947.0 (26.94% identity, 41.65%similarity) APG08243.0 (26.69% identity, 44.32% similarity) APG03699.0(26.61% identity, 39.21% similarity) APG00435.0 US_2016_0366881_A1-59(26.32% identity, 41.02% similarity) WP_086420153.1 (26.32% identity,41.02% similarity) APG01860.0 75, 80, 85, 90, 91, 92, APG09282.0 (70.13%identity, 72.08% 93, 94, 95, 96, 97, similarity) 98, 99, 100 APG00787.0US_2017_0175134_A1-156 (59.16% identity, 67.39% similarity) APG05538.0(57.65% identity, 63.19% similarity) APG02533.0 (57.1% identity, 63.71%similarity) KXH80331.1 (49.46% identity, 57.98% similarity)WP_082770008.1 (47.97% identity, 56.24% similarity) WP_098395829.1(46.46% identity, 55.43% similarity) APG05689.0 (45.7% identity, 57.56%similarity) APG05439.0 (45.03% identity, 57.71% similarity) APG09630.0(44.97% identity, 52.9% similarity) APG01967.0 90, 91, 92, 93, 94, 95,US_2015_0047076_A1-8 (76.33% identity, 96, 97, 98, 99, 100 85.87%similarity) US_2015_0047076_A1-4 (68.06% identity, 76.39% similarity)US_2015_0047076_A1-10 (67.49% identity, 77.39% similarity) AQM56940.1(65.37% identity, 72.44% similarity) US_2015_0047076_A1-2 (64.81%identity, 75.96% similarity) US_8461421_B2-42 (64.44% identity, 74.65%similarity) US_8461421_B2-102 (64.08% identity, 74.65% similarity)US_2015_0047076_A1-12 (64.01% identity, 76.47% similarity)US_8461421_B2-220 (63.73% identity, 73.94% similarity) US_9567381_B2-384(62.94% identity, 74.83% similarity) APG02071.0 60, 65, 70, 75, 80, 85,APG05073.0 (45.78% identity, 58.49% 90, 91, 92, 93, 94, similarity) 95,96, 97, 98, 99, CA_2595901-12 (44.9% identity, 56.86% 100 similarity)US_5281530-4 (44.64% identity, 57.26% similarity) CA_2595901-11 (44.52%identity, 57.37% similarity) US_5281530-3 (44.52% identity, 57.37%similarity) WP_097983832.1 (44.24% identity, 55.97% similarity)US_9567381_B2-433 (44.17% identity, 55.9% similarity) WP_098856496.1(44.17% identity, 55.83% similarity) US_9567381_B2-434 (44.03% identity,55.83% similarity) APG00622.0 US_2017_0175134_A1-58 (43.68% identity,55.38% similarity) APG00622.0 US_2017_0175134_A1-58 (43.68% identity,55.38% similarity) APG02105.0 99, 100 WP_088080922.1 (97.05% identity,97.7% similarity) APG06465.0 (96.72% identity, 97.7% similarity)APG01882.0 (96.41% identity, 97.39% similarity) WP_098290464.1 (96.41%identity, 97.39% similarity) APG08138.0 (96.39% identity, 98.36%similarity) APG04483.0 (96.39% identity, 97.38% similarity)WP_098908548.1 (96.39% identity, 97.38% similarity) APG00696.0US_2016_0355842_A1-160 (96.39% identity, 97.05% similarity)WP_098222384.1 (96.39% identity, 97.05% similarity) WP_098162507.1(96.07% identity, 97.05% similarity) WP_098778568.1 (95.74% identity,97.05% similarity) APG01265.0 (95.41% identity, 97.38% similarity)APG06195.0 (95.08% identity, 96.07% similarity) APG03715.0 (91.25%identity, 92.5% similarity) WP_087969960.1 (90.6% identity, 91.85%similarity) APG02110.0 90, 91, 92, 93, 94, 95, APG06213.0 (95.78%identity, 97.89% 96, 97, 98, 99, 100 similarity) APG07811.0 (95.61%identity, 97.54% similarity) APG06606.0 (95.08% identity, 97.19%similarity) APG04108.0 (83.45% identity, 88.33% similarity) APG00235.0US_2016_0311864_A1-89 (82.87% identity, 88.75% similarity)WP_050845493.1 (64.12% identity, 75.51% similarity) APG08681.0 (55.49%identity, 66.3% similarity) WP_048536324.1 (55.49% identity, 66.3%similarity) APG00702.0 US_2017_0175134_A1-115 (37.52% identity, 50.72%similarity) APG00401.0 US_2016_0311864_A1-168 (33.78% identity, 45.05%similarity) APG00401.0 US_2016_0311864_A1-168 (33.78% identity, 45.05%similarity) APG02115.0 90, 91, 92, 93, 94, 95, APG04978.0 (80.08%identity, 87.22% 96, 97, 98, 99, 100 similarity) APG08577.0 (42.81%identity, 62.23% similarity) SHH89794.1 (37.28% identity, 55.91%similarity) WP_073006288.1 (35.56% identity, 55.99% similarity)WP_084723762.1 (34.33% identity, 52.0% similarity) APG07463.0 (31.4%identity, 52.22% similarity) WP_073008904.1 (30.8% identity, 49.83%similarity) WP_073006284.1 (27.18% identity, 50.17% similarity)APG02143.0 70, 75, 80, 85, 90, 91, WP_098099663.1 (49.39% identity,65.55% 92, 93, 94, 95, 96, similarity) 97, 98, 99, 100 WP_098087996.1(49.39% identity, 65.24% similarity) US_9328356_B2-26 (49.09% identity,65.55% similarity) WP_098179800.1 (49.09% identity, 65.55% similarity)WP_098643733.1 (49.09% identity, 65.24% similarity) WP_098072309.1(48.78% identity, 65.24% similarity) APG00866.0 US_2016_0355842_A1-183(47.15% identity, 63.66% similarity) WP_098072344.1 (47.15% identity,63.66% similarity) US_9567381_B2-328 (42.51% identity, 60.18%similarity) US_9238678_B2-123 (34.77% identity, 49.43% similarity)US_9238678_B2-125 (34.77% identity, 49.43% similarity) APG02166.0 60,65, 70, 75, 80, 85, APG06578.0 (44.26% identity, 60.98% 90, 91, 92, 93,94, similarity) 95, 96, 97, 98, 99, APG09957.0 (40.65% identity, 57.74%100 similarity) APG02633.0 (32.4% identity, 50.17% similarity)APG06768.0 (30.72% identity, 48.28% similarity) APG02714.0 (29.15%identity, 49.83% similarity) APG05793.0 (25.08% identity, 41.69%similarity) APG02206.0 94, 95, 96, 97, 98, 99, APG00932.0 (89.95%identity, 93.47% 100 similarity) APG03217.0 (76.63% identity, 85.18%similarity) APG08029.0 (76.13% identity, 85.18% similarity)WP_098069633.1 (75.63% identity, 84.17% similarity) WP_098099018.1(75.63% identity, 84.17% similarity) WP_098087707.1 (75.38% identity,84.17% similarity) WP_099010057.1 (75.38% identity, 83.92% similarity)APG01797.0 (75.13% identity, 84.42% similarity) APG04119.0 (62.41%identity, 77.19% similarity) APG06283.0 (61.19% identity, 75.12%similarity) APG02221.0 75, 80, 85, 90, 91, 92, APG01288.0 (59.56%identity, 70.17% 93, 94, 95, 96, 97, similarity) 98, 99, 100WP_098214356.1 (59.56% identity, 70.17% similarity) APG07636.0 (52.3%identity, 66.81% similarity) APG00801.0 US_2017_0175134_A1-160 (49.38%identity, 61.98% similarity) APG00784.0 US_2017_0175134_A1-148 (48.35%identity, 60.23% similarity) APG00099.0 US_2016_0177333_A1-55 (46.67%identity, 58.75% similarity) WP_098487817.1 (46.67% identity, 58.75%similarity) US_2011_0239334_A1-82 (31.0% identity, 43.74% similarity)US_2011_0239334_A1-84 (30.99% identity, 44.12% similarity) APG00217.0US_2016_0355842_A1-24 (30.25% identity, 39.51% similarity) APG02256.099, 100 APG07058.0 (98.4% identity, 98.94% similarity) WP_078404291.1(97.87% identity, 98.4% similarity) APG04807.0 (32.41% identity, 50.5%similarity) OPA24196.1 (31.92% identity, 49.63% similarity)WP_078404344.1 (30.7% identity, 47.72% similarity) APG05429.0 (30.62%identity, 47.85% similarity) APG09864.0 (30.16% identity, 46.34%similarity) APG08307.0 (30.0% identity, 50.5% similarity) APG00940.0US_2016_0355842_A1-191 (28.57% identity, 45.76% similarity)WP_098783549.1 (28.57% identity, 45.76% similarity) APG02309.0 50, 55,60, 65, 70, 75, APG09289.0 (65.9% identity, 75.48% 80, 85, 90, 91, 92,similarity) 93, 94, 95, 96, 97, APG09289.0 (65.9% identity, 75.48% 98,99, 100 similarity) US_5281530-4 (35.25% identity, 49.16% similarity)US_5281530-4 (35.25% identity, 49.16% similarity) US_5281530-4 (35.25%identity, 49.16% similarity) CA_2595901-11 (35.21% identity, 49.43%similarity) CA_2595901-11 (35.21% identity, 49.43% similarity)US_5281530-3 (35.21% identity, 49.43% similarity) US_5281530-3 (35.21%identity, 49.43% similarity) CA_2595901-10 (34.97% identity, 49.87%similarity) CA_2595901-10 (34.97% identity, 49.87% similarity)APG02315.0 80, 85, 90, 91, 92, 93, APG00541.0 US_2016_0355842_A1-119 94,95, 96, 97, 98, (70.91% identity, 79.39% similarity) 99, 100WP_097855808.1 (70.91% identity, 79.39% similarity) WP_098581475.1(70.91% identity, 79.39% similarity) APG00569.0 US_2016_0355842_A1-129(70.38% identity, 78.01% similarity) WP_098115624.1 (70.38% identity,78.01% similarity) APG00509.0 US_2016_0355842_A1-113 (70.3% identity,79.09% similarity) APG00244.0 US_2016_0355842_A1-33 (66.97% identity,76.67% similarity) WP_098381606.1 (66.97% identity, 76.67% similarity)WP_098213528.1 (66.67% identity, 76.67% similarity) APG06371.0 (66.08%identity, 74.56% similarity) APG02319.0 92, 93, 94, 95, 96, 97,APG00614.0 US_2016_0355842_A1-144 98, 99, 100 (81.54% identity, 91.15%similarity) WP_098072343.1 (81.54% identity, 91.15% similarity)PEJ82417.1 (81.23% identity, 90.04% similarity) US_9328356_B2-22 (79.23%identity, 88.46% similarity) AAL26870.1 (64.66% identity, 78.95%similarity) APG05300.0 (34.77% identity, 52.33% similarity) APG08525.0(34.39% identity, 51.93% similarity) US_5308760_A-9 (34.29% identity,48.25% similarity) APG02740.0 (34.28% identity, 55.12% similarity)APG06236.0 (33.82% identity, 50.91% similarity) APG02380.0 96, 97, 98,99, 100 CA_2442594-2 (91.89% identity, 95.44% similarity)US_9567381_B2-1539 (91.89% identity, 95.44% similarity) AIT93171.1(91.89% identity, 95.18% similarity) AIT93180.1 (91.89% identity, 95.06%similarity) CA_2442594-3 (91.76% identity, 95.31% similarity) AIT93181.1(91.76% identity, 95.06% similarity) AIT93176.1 (91.76% identity, 94.8%similarity) AIT93170.1 (91.63% identity, 95.06% similarity) CA_2479769-2(91.63% identity, 94.8% similarity) US_9567381_B2-1536 (91.51% identity,95.18% similarity) KIP27841.1 (91.51% identity, 95.06% similarity)CA_2327266-54 (90.75% identity, 94.8% similarity) US_7129212_B2-27(90.62% identity, 94.68% similarity) CA_2442594-7 (90.24% identity,94.3% similarity) APG02451.0 98, 99, 100 APG00987.0US_2016_0366881_A1-223 (94.88% identity, 97.95% similarity) APG00506.0US_2016_0366881_A1-102 (91.3% identity, 94.98% similarity) APG04821.0(91.3% identity, 94.98% similarity) US_8461415_B2-57 (50.83% identity,69.77% similarity) APG00697.0 US_2016_0366881_A1-222 (48.49% identity,68.9% similarity) APG00346.0 US_2016_0366881_A1-24 (48.16% identity,68.9% similarity) US_8461415_B2-56 (45.27% identity, 67.91% similarity)APG00450.0 US_2016_0366881_A1-72 (43.41% identity, 63.34% similarity)APG01761.0 (43.09% identity, 62.7% similarity) APG05804.0 (41.88%identity, 61.04% similarity) APG02475.0 98, 99, 100 APG04431.0 (96.18%identity, 97.71% similarity) CA_2844913-120 (88.55% identity, 92.62%similarity) APG05346.0 (88.04% identity, 92.62% similarity) APG00202.0US_2016_0304898_A1-195 (87.12% identity, 92.93% similarity)WP_098111055.1 (86.77% identity, 92.11% similarity) APG01443.0 (86.55%identity, 92.89% similarity) APG00208.0 US_2016_0304898_A1-201 (82.91%identity, 90.45% similarity) APG00352.0 US_2016_0366881_A1-29 (77.47%identity, 87.09% similarity) APG07094.0 (76.96% identity, 86.84%similarity) WP_000715637.1 (75.89% identity, 83.5% similarity)APG02506.0 75, 80, 85, 90, 91, 92, US_8318900_B2-91 (62.64% identity,93, 94, 95, 96, 97, 72.07% similarity) 98, 99, 100 PGZ56209.1 (58.82%identity, 66.39% similarity) APG08243.0 (55.56% identity, 67.0%similarity) APG01679.0 (42.06% identity, 55.62% similarity)WP_048536362.1 (42.06% identity, 55.62% similarity) APG00375.0US_2016_0311864_A1-152 (41.38% identity, 55.07% similarity)WP_098805991.1 (41.38% identity, 55.07% similarity) APG05139.0 (39.74%identity, 53.96% similarity) APG07224.0 (39.48% identity, 52.69%similarity) US_8318900_B2-90 (39.07% identity, 53.7% similarity)APG02511.0 70, 75, 80, 85, 90, 91, APG02659.0 (56.98% identity, 67.88%92, 93, 94, 95, 96, similarity) 97, 98, 99, 100 WP_078401252.1 (53.93%identity, 64.89% similarity) US_8461415_B2-32 (53.33% identity, 65.51%similarity) WP_098213667.1 (53.3% identity, 69.63% similarity)WP_098487632.1 (53.3% identity, 69.63% similarity) JP_2016_516397-59(53.07% identity, 65.64% similarity) ANS51604.1 (52.79% identity, 65.36%similarity) AGC39300.1 (52.23% identity, 64.04% similarity)JP_2016_516397-60 (51.96% identity, 64.25% similarity) WP_023524027.1(51.69% identity, 62.43% similarity) APG02587.0 95, 96, 97, 98, 99, 100US_7923602_B2-2 (90.97% identity, 94.85% similarity) US_7923602_B2-17(90.8% identity, 94.77% similarity) US_7923602_B2-33 (90.66% identity,94.53% similarity) US_7923602_B2-38 (88.42% identity, 92.28% similarity)APG00052.0 US_2016_0177333_A1-25 (87.5% identity, 93.16% similarity)US_7923602_B2-13 (84.78% identity, 89.91% similarity)US_2011_0231963_A1-6 (84.63% identity, 89.76% similarity) US_5589382_A-4(84.61% identity, 89.82% similarity) US_9139843_B2-1 (84.5% identity,90.23% similarity) US_5959080_A-3 (84.19% identity, 89.32% similarity)APG02592.0 55, 60, 65, 70, 75, 80, APG07732.0 (45.49% identity, 66.32%85, 90, 91, 92, 93, similarity) 94, 95, 96, 97, 98, APG02088.0 (33.64%identity, 52.47% 99, 100 similarity) APG07824.0 (32.61% identity, 50.62%similarity) APG05918.0 (32.24% identity, 50.33% similarity) APG09455.0(31.69% identity, 48.62% similarity) APG09675.0 (31.33% identity, 50.32%similarity) APG00598.0 US_2016_0355842_A1-140 (30.87% identity, 50.16%similarity) APG06880.0 (28.66% identity, 44.48% similarity) APG02633.0(28.43% identity, 46.08% similarity) APG00236.0 US_2016_0355842_A1-32(25.63% identity, 46.84% similarity) APG02656.0 5, 10, 15, 20, 25, 30,WP_039580723.1 (74.55% identity, 81.91% 35, 40, 45, 50, 55, similarity)60, 65, 70, 75, 80, 85, WP_023629461.1 (60.82% identity, 72.9% 90, 91,92, 93, 94, similarity) 95, 96, 97, 98, 99, WP_094012300.1 (60.62%identity, 72.71% 100 similarity) WP_104444565.1 (60.43% identity, 72.51%similarity) WP_073183820.1 (30.93% identity, 49.03% similarity)WP_074623349.1 (28.18% identity, 44.55% similarity) WP_088716757.1(27.69% identity, 43.39% similarity) WP_043145958.1 (27.26% identity,43.01% similarity) WP_088671570.1 (27.26% identity, 43.01% similarity)WP_069210391.1 (27.26% identity, 42.83% similarity) APG02659.0 75, 80,85, 90, 91, 92, JP_2016_516397-59 (58.94% identity, 93, 94, 95, 96, 97,70.39% similarity) 98, 99, 100 WP_078401252.1 (58.47% identity, 69.49%similarity) ANS51604.1 (58.38% identity, 69.83% similarity)JP_2016_516397-60 (57.26% identity, 68.72% similarity) APG02511.0(56.98% identity, 67.88% similarity) WP_098213667.1 (56.5% identity,68.64% similarity) WP_098487632.1 (56.5% identity, 68.36% similarity)JP_2016_516397-61 (56.42% identity, 67.6% similarity) WP_023524027.1(56.02% identity, 69.47% similarity) JP_2016_516397-63 (53.54% identity,64.59% similarity) APG02671.0 90, 91, 92, 93, 94, 95, APG00015.0US_2016_0304898_A1-25 96, 97, 98, 99, 100 (84.27% identity, 89.16%similarity) WP_098207008.1 (84.27% identity, 89.16% similarity)APG00468.0 US_2016_0366881_A1-81 (84.27% identity, 88.81% similarity)APG01301.0 (76.77% identity, 82.83% similarity) APG01121.0 (75.61%identity, 84.32% similarity) APG00168.0 US_2016_0311864_A1-44 (61.02%identity, 73.56% similarity) APG00790.0 (59.33% identity, 73.33%similarity) APG00431.0 US_2016_0366881_A1-56 (59.06% identity, 74.16%similarity) APG01257.0 (59.06% identity, 73.15% similarity)US_2017_0058293_A1-4 (58.94% identity, 69.87% similarity) APG02676.0 5,10, 15, 20, 25, 30, WP_078782981.1 (94.07% identity, 95.36% 35, 40, 45,50, 55, similarity) 60, 65, 70, 75, 80, 85, WP_073552443.1 (89.02%identity, 90.24% 90, 91, 92, 93, 94, similarity) 95, 96, 97, 98, 99,AIL46026.1 (64.97% identity, 81.22% 100 similarity) CDN80307.1 (64.72%identity, 80.96% similarity) WP_024564198.1 (62.75% identity, 78.43%similarity) WP_078724005.1 (62.75% identity, 78.43% similarity)WP_035592229.1 (62.5% identity, 78.19% similarity) WP_078675442.1(62.25% identity, 78.19% similarity) AQW99431.1 (62.25% identity, 77.94%similarity) WP_078720018.1 (62.01% identity, 77.94% similarity)APG02706.0 60, 65, 70, 75, 80, 85, APG00651.0 US_2017_0175134_A1-77 90,91, 92, 93, 94, (45.86% identity, 59.49% similarity) 95, 96, 97, 98, 99,APG00524.0 US_2017_0175134_A1-17 100 (44.69% identity, 57.21%similarity) APG00703.0 US_2017_0175134_A1-117 (42.5% identity, 56.25%similarity) AHI15917.1 (41.41% identity, 57.03% similarity)CA_2595901-15 (41.16% identity, 57.42% similarity) AHI15915.1 (40.71%identity, 55.81% similarity) US_8318900_B2-68 (40.37% identity, 56.1%similarity) AEH76817.1 (40.15% identity, 56.18% similarity)WP_080685557.1 (39.37% identity, 53.97% similarity)US_2011_0239334_A1-79 (39.24% identity, 55.45% similarity)US_2011_0239334_A1-79 (39.24% identity, 55.45% similarity)US_5670365_A-9 (39.24% identity, 55.45% similarity) US_5670365_A-9(39.24% identity, 55.45% similarity) US_9567381_B2-1403 (39.24%identity, 55.45% similarity) US_9567381_B2-1403 (39.24% identity, 55.45%similarity) APG02714.0 85, 90, 91, 92, 93, 94, APG05793.0 (83.68%identity, 84.03% 95, 96, 97, 98, 99, similarity) 100 APG06768.0 (71.48%identity, 84.19% similarity) APG02633.0 (40.82% identity, 60.88%similarity) APG04111.0 (31.21% identity, 50.34% similarity) APG04977.0(30.46% identity, 49.01% similarity) APG09675.0 (30.0% identity, 50.0%similarity) APG06578.0 (29.69% identity, 44.06% similarity) APG00236.0US_2016_0355842_A1-32 (28.76% identity, 51.31% similarity) APG09957.0(28.26% identity, 49.38% similarity) APG02718.0 45, 50, 55, 60, 65, 70,AQT70550.1 (51.15% identity, 65.14% 75, 80, 85, 90, 91, similarity) 92,93, 94, 95, 96, EFL12798.1 (50.8% identity, 65.9% 97, 98, 99, 100similarity) WP_030873518.1 (49.54% identity, 64.68% similarity)WP_093835703.1 (45.35% identity, 60.32% similarity) WP_078093242.1(43.55% identity, 56.07% similarity) WP_078857801.1 (41.51% identity,53.51% similarity) WP_082206325.1 (39.37% identity, 51.97% similarity)WP_073808998.1 (34.15% identity, 46.54% similarity) WP_100584951.1(28.62% identity, 40.34% similarity) PJN29953.1 (28.62% identity, 40.17%similarity) APG02767.0 94, 95, 96, 97, 98, 99, WP_095210724.1 (89.7%identity, 93.55% 100 similarity) KMQ16234.1 (89.49% identity, 93.34%similarity) APG00939.0 US_2017_0175134_A1-49 (88.76% identity, 93.55%similarity) APG00077.0 US_2016_0304898_A1-99 (85.43% identity, 91.05%similarity) APG00278.0 US_2016_0311864_A1-112 (83.94% identity, 88.39%similarity) APG00175.0 US_2016_0311864_A1-48 (83.77% identity, 89.28%similarity) WP_098343719.1 (83.77% identity, 89.28% similarity)APG00173.0 US_2016_0311864_A1-46 (81.75% identity, 87.11% similarity)WP_098839023.1 (81.75% identity, 87.11% similarity) APG00875.0US_2016_0366881_A1-133 (81.13% identity, 86.49% similarity) APG02941.090, 91, 92, 93, 94, 95, APG02038.0 (84.19% identity, 89.03% 96, 97, 98,99, 100 similarity) WP_098584414.1 (84.19% identity, 89.03% similarity)APG03662.0 (83.01% identity, 87.18% similarity) PGM93662.1 (67.48%identity, 76.69% similarity) APG06508.0 US_2017_0175134_A1-217 (61.45%identity, 72.59% similarity) US_8829279_B2-25 (61.38% identity, 73.05%similarity) APG09801.0 US_2017_0175134_A1-218 (61.26% identity, 73.87%similarity) APG00661.0 US_2017_0175134_A1-83 (60.6% identity, 71.94%similarity) US_8318900_B2-62 (58.79% identity, 71.21% similarity)US_8829279_B2-35 (58.01% identity, 70.69% similarity) APG02947.0 96, 97,98, 99, 100 APG00536.0 US_2017_0175134_A1-24 (94.37% identity, 95.81%similarity) APG00046.0 US_2016_0177333_A1-20 (87.19% identity, 92.54%similarity) JP_2016_516397-52 (87.19% identity, 92.54% similarity)JP_2016_516397-9 (87.19% identity, 92.54% similarity) JP_2016_516397-53(86.94% identity, 92.29% similarity) WP_098712089.1 (83.58% identity,88.56% similarity) WP_098995610.1 (81.34% identity, 86.19% similarity)JP_2016_516397-54 (79.1% identity, 83.58% similarity) JP_2016_516397-55(78.23% identity, 82.59% similarity) JP_2016_516397-56 (76.74% identity,81.09% similarity) APG02956.0 80, 85, 90, 91, 92, 93, APG03565.0 (62.8%identity, 77.13% 94, 95, 96, 97, 98, similarity) 99, 100 APG06065.0(62.33% identity, 77.05% similarity) US_2003_0049243_A1-38 (36.67%identity, 56.33% similarity) WP_042595209.1 (36.67% identity, 56.33%similarity) US_9328356_B2-54 (33.02% identity, 52.02% similarity)WP_002193629.1 (31.11% identity, 47.3% similarity) APG00871.0 (29.02%identity, 45.43% similarity) US_2003_0049243_A1-45 (29.02% identity,44.59% similarity) APG02986.0 65, 70, 75, 80, 85, 90, APG00534.0US_2017_0175134_A1-22 91, 92, 93, 94, 95, (51.79% identity, 61.85%similarity) 96, 97, 98, 99, 100 APG00120.0 US_2016_0177333_A1-68 (49.32%identity, 58.62% similarity) WP_098164683.1 (49.21% identity, 58.62%similarity) US_8461421_B2-204 (47.67% identity, 64.93% similarity)WP_098905715.1 (47.2% identity, 59.01% similarity) APG08350.0 (44.25%identity, 55.63% similarity) APG06056.0 (43.8% identity, 56.08%similarity) APG02109.0 (43.53% identity, 54.39% similarity)WP_088125814.1 (42.48% identity, 54.28% similarity) US_8461421_B2-264(42.38% identity, 54.16% similarity) APG03018.0 90, 91, 92, 93, 94, 95,WP_085313394.1 (99.24% identity, 99.24% 96, 97, 98, 99, 100 similarity)WP_103592286.1 (85.93% identity, 89.86% similarity) US_8318900_B2-9(83.73% identity, 88.1% similarity) US_8318900_B2-58 (83.58% identity,88.1% similarity) US_8318900_B2-113 (83.28% identity, 87.65% similarity)US_7919272_B2-19 (81.54% identity, 85.33% similarity) US_7919272_B2-35(81.24% identity, 85.02% similarity) WP_050845711.1 (76.16% identity,83.51% similarity) US_9567381_B2-427 (75.78% identity, 83.11%similarity) AGE10586.1 (75.56% identity, 83.06% similarity) APG03054.065, 70, 75, 80, 85, 90, ANC11094.1 (66.62% identity, 79.06% 91, 92, 93,94, 95, similarity) 96, 97, 98, 99, 100 WP_080470008.1 (65.67% identity,78.06% similarity) APG04651.0 (61.03% identity, 74.92% similarity)APG06921.0 (43.63% identity, 62.08% similarity) CBL59394.1 (42.94%identity, 61.38% similarity) CBL59393.1 (42.94% identity, 61.24%similarity) CBL59395.1 (42.94% identity, 61.24% similarity) CBL59396.1(42.94% identity, 61.24% similarity) CBL59397.1 (42.94% identity, 61.1%similarity) APG09814.0 (41.57% identity, 57.0% similarity) APG03056.070, 75, 80, 85, 90, 91, APG08884.0 (57.11% identity, 68.45% 92, 93, 94,95, 96, similarity) 97, 98, 99, 100 APG02841.0 (43.42% identity, 58.1%similarity) APG00162.0 US_2016_0177333_A1-82 (43.36% identity, 56.56%similarity) WP_098826687.1 (43.36% identity, 56.56% similarity)APG00751.0 (42.69% identity, 58.27% similarity) APG00374.0US_2016_0366881_A1-40 (42.34% identity, 57.99% similarity)US_9567381_B2-413 (39.08% identity, 54.93% similarity) APG00326.0US_2017_0175134_A1-1 (38.96% identity, 54.64% similarity)US_9567381_B2-411 (38.63% identity, 54.17% similarity) AAW55474.1(38.37% identity, 55.46% similarity) APG03182.0 80, 85, 90, 91, 92, 93,APG06372.0 (63.24% identity, 77.7% 94, 95, 96, 97, 98, similarity) 99,100 WP_078185377.1 (63.24% identity, 77.7% similarity) WP_098954578.1(63.24% identity, 77.7% similarity) APG00129.0 US_2016_0304898_A1-158(63.24% identity, 77.21% similarity) WP_097908072.1 (63.24% identity,77.21% similarity) OQR53340.1 (62.99% identity, 77.21% similarity)KXY21848.1 (62.75% identity, 78.19% similarity) CDN39409.1 (62.75%identity, 77.94% similarity) APG00923.0 (62.75% identity, 77.45%similarity) APG03337.0 (62.75% identity, 77.21% similarity) APG03218.065, 70, 75, 80, 85, 90, APG01201.0 (52.54% identity, 68.08% 91, 92, 93,94, 95, similarity) 96, 97, 98, 99, 100 APG00966.0US_2016_0355842_A1-195 (45.74% identity, 62.78% similarity)WP_098976737.1 (45.74% identity, 62.78% similarity) WP_098139851.1(44.35% identity, 62.15% similarity) APG00479.0 US_2016_0355842_A1-101(43.97% identity, 58.91% similarity) WP_098139684.1 (43.97% identity,58.91% similarity) APG00283.0 US_2016_0355842_A1-50 (43.37% identity,60.77% similarity) APG03623.0 (41.41% identity, 58.59% similarity)WP_065486138.1 (40.63% identity, 59.94% similarity) WP_065487080.1(40.63% identity, 59.94% similarity) APG03247.0 90, 91, 92, 93, 94, 95,WP_069848556.1 (88.07% identity, 90.66% 96, 97, 98, 99, 100 similarity)WP_015892933.1 (87.68% identity, 90.4% similarity) WP_017251277.1(87.42% identity, 90.27% similarity) APG05519.0 (87.42% identity, 89.88%similarity) WP_087343164.1 (84.05% identity, 88.07% similarity)APG08067.0 (83.92% identity, 87.55% similarity) WP_016742208.1 (83.4%identity, 87.42% similarity) APG00182.0 US_2016_0355842_A1-17 (26.59%identity, 39.66% similarity) CA_2595901-6 (25.7% identity, 39.91%similarity) APG05711.0 (25.6% identity, 39.43% similarity) APG03361.060, 65, 70, 75, 80, 85, APG05955.0 (64.06% identity, 75.59% 90, 91, 92,93, 94, similarity) 95, 96, 97, 98, 99, APG09146.0 (42.31% identity,57.09% 100 similarity) WP_016099737.1 (40.75% identity, 53.77%similarity) US_8461415_B2-29 (36.69% identity, 50.47% similarity)US_8461415_B2-44 (33.39% identity, 46.48% similarity) APG06401.0 (32.13%identity, 45.63% similarity) APG08101.0 (31.96% identity, 48.97%similarity) APG07432.0 (31.53% identity, 46.83% similarity)WP_098826686.1 (30.09% identity, 45.87% similarity) APG00734.0US_2016_0366881_A1-131 (30.02% identity, 45.72% similarity) APG03392.085, 90, 91, 92, 93, 94, APG00266.0 US_2016_0311864_A1-105 95, 96, 97,98, 99, (73.65% identity, 82.0% similarity) 100 APG00288.0US_2016_0311864_A1-122 (58.78% identity, 72.19% similarity)WP_098783882.1 (58.78% identity, 72.19% similarity) APG06730.0 (48.6%identity, 61.2% similarity) APG00043.0 US_2016_0304898_A1-69 (34.62%identity, 48.72% similarity) APG00396.0 US_2016_0311864_A1-163 (33.06%identity, 44.35% similarity) WP_098783217.1 (33.06% identity, 44.35%similarity) APG06774.0 (31.87% identity, 48.59% similarity)US_8318900_B2-91 (31.54% identity, 47.31% similarity) APG00372.0US_2016_0311864_A1-150 (31.25% identity, 46.14% similarity) APG03415.065, 70, 75, 80, 85, 90, APG00360.0 US_2016_0355842_A1-69 91, 92, 93, 94,95, (44.38% identity, 60.79% similarity) 96, 97, 98, 99, 100 APG00907.0US_2016_0311864_A1-225 (39.17% identity, 58.75% similarity)WP_098908553.1 (39.17% identity, 58.75% similarity) US_8318900_B2-191(39.16% identity, 59.94% similarity) APG01122.0 (39.02% identity, 57.62%similarity) APG05358.0 (38.37% identity, 57.1% similarity) APG01885.0(38.18% identity, 57.58% similarity) APG02548.0 (37.87% identity, 57.69%similarity) APG07396.0 (37.83% identity, 54.25% similarity) APG04690.0(37.61% identity, 56.42% similarity) APG03504.0 97, 98, 99, 100APG01127.0 (92.93% identity, 96.97% similarity) APG07655.0 (92.91%identity, 96.62% similarity) APG00155.0 US_2016_0311864_A1-40 (92.57%identity, 96.62% similarity) APG03379.0 (82.21% identity, 89.6%similarity) APG01506.0 (81.76% identity, 88.51% similarity) APG00749.0(81.13% identity, 88.08% similarity) APG09416.0 (79.93% identity, 86.62%similarity) APG08241.0 (79.73% identity, 86.49% similarity) APG09682.0(79.12% identity, 86.87% similarity) APG02638.0 (79.07% identity, 88.04%similarity) APG03505.0 85, 90, 91, 92, 93, 94, US_7923602_B2-6 (72.6%identity, 81.87% 95, 96, 97, 98, 99, similarity) 100 US_7923602_B2-35(48.35% identity, 59.32% similarity) US_8318900_B2-68 (46.78% identity,59.51% similarity) AHI15917.1 (46.73% identity, 59.29% similarity)AHI15915.1 (46.44% identity, 58.34% similarity) AEH76817.1 (46.2%identity, 59.36% similarity) AHI15916.1 (45.71% identity, 57.14%similarity) US_9567381_B2-1403 (45.29% identity, 55.0% similarity)US_9567381_B2-1403 (45.29% identity, 55.0% similarity)WO_2017_161181-215 (45.29% identity, 55.0% similarity)WO_2017_161181-215 (45.29% identity, 55.0% similarity) APG03527.0 94,95, 96, 97, 98, 99, US_2015_0047076_A1-14 (88.77% identity, 100 93.84%similarity) US_2015_0047076_A1-8 (57.14% identity, 70.73% similarity)APG01967.0 (56.34% identity, 70.07% similarity) US_2015_0047076_A1-12(55.36% identity, 67.82% similarity) US_2015_0047076_A1-10 (52.28%identity, 67.72% similarity) US_2015_0047076_A1-4 (52.08% identity,65.97% similarity) AQM56940.1 (51.6% identity, 62.63% similarity)US_2015_0047076_A1-2 (50.53% identity, 65.26% similarity)US_2015_0047076_A1-6 (50.0% identity, 66.07% similarity)US_8461421_B2-42 (49.82% identity, 67.26% similarity) APG03584.0 60, 65,70, 75, 80, 85, WP_075719043.1 (37.59% identity, 56.26% 90, 91, 92, 93,94, similarity) 95, 96, 97, 98, 99, APG05777.0 (37.36% identity, 56.26%100 similarity) WP_100910639.1 (37.36% identity, 56.26% similarity)APG00129.0 US_2016_0304898_A1-158 (36.94% identity, 53.38% similarity)WP_097908072.1 (36.94% identity, 53.38% similarity) APG04650.0 (36.71%identity, 53.6% similarity) KXY21848.1 (36.04% identity, 53.38%similarity) CDN39409.1 (36.04% identity, 53.15% similarity)WP_078185377.1 (36.04% identity, 53.15% similarity) APG02686.0 (35.81%identity, 53.6% similarity) WP_080448724.1 (35.81% identity, 53.6%similarity) APG02421.0 (35.81% identity, 53.38% similarity)WP_080685552.1 (35.81% identity, 53.38% similarity) APG03337.0 (35.81%identity, 53.15% similarity) OQR53340.1 (35.81% identity, 53.15%similarity) APG03587.0 90, 91, 92, 93, 94, 95, APG09439.0 (92.83%identity, 96.42% 96, 97, 98, 99, 100 similarity) APG07780.0 (76.34%identity, 85.66% similarity) APG05984.0 (75.99% identity, 85.3%similarity) APG08576.0 (73.84% identity, 84.95% similarity) APG01093.0(72.4% identity, 81.72% similarity) APG05943.0 (68.58% identity, 79.39%similarity) APG01273.0 (67.02% identity, 78.25% similarity) APG08917.0(58.06% identity, 69.89% similarity) APG01706.0 (52.69% identity, 66.31%similarity) APG05992.0 (51.97% identity, 68.1% similarity) APG03674.0100 APG09444.0 (95.59% identity, 99.51% similarity) APG01987.0 (94.12%identity, 98.53% similarity) US_2016_0339078_A1-28662 (94.12% identity,98.53% similarity) WP_018782198.1 (94.12% identity, 98.53% similarity)WP_033798332.1 (94.12% identity, 98.53% similarity) WP_040119538.1(94.12% identity, 98.53% similarity) WP_016114769.1 (94.12% identity,98.04% similarity) WP_018766353.1 (94.12% identity, 98.04% similarity)WP_098928754.1 (93.63% identity, 98.53% similarity)US_2016_0339078_A1-28716 (93.63% identity, 98.04% similarity)WP_097849755.1 (93.63% identity, 98.04% similarity) WP_098114905.1(93.63% identity, 98.04% similarity) WP_097836653.1 (93.63% identity,97.55% similarity) APG05707.0 (93.14% identity, 97.55% similarity)APG00770.0 (93.14% identity, 96.08% similarity) APG01989.0 (91.67%identity, 96.08% similarity) APG00456.0 US_2016_0311864_A1-180 (90.69%identity, 96.08% similarity) APG03738.0 85, 90, 91, 92, 93, 94,APG04977.0 (74.45% identity, 86.13% 95, 96, 97, 98, 99, similarity) 100APG02471.0 (71.74% identity, 82.97% similarity) APG04111.0 (57.04%identity, 72.51% similarity) APG00236.0 US_2016_0355842_A1-32 (48.28%identity, 67.24% similarity) APG02633.0 (33.33% identity, 53.33%similarity) APG00598.0 US_2016_0355842_A1-140 (31.7% identity, 50.65%similarity) APG02714.0 (31.63% identity, 50.16% similarity) APG05793.0(29.77% identity, 46.49% similarity) APG07732.0 (29.71% identity, 49.84%similarity) APG03748.0 96, 97, 98, 99, 100 APG00229.0US_2016_0311864_A1-84 (91.58% identity, 95.3% similarity) APG07334.0(89.58% identity, 93.3% similarity) WP_098205870.1 (89.36% identity,93.07% similarity) APG00419.0 US_2016_0311864_A1-176 (88.7% identity,92.14% similarity) WP_098634587.1 (88.7% identity, 92.14% similarity)APG00203.0 US_2016_0311864_A1-69 (87.84% identity, 90.32% similarity)WP_098475832.1 (87.84% identity, 90.32% similarity) APG05411.0 (86.88%identity, 91.34% similarity) APG00353.0 US_2016_0311864_A1-144 (86.1%identity, 88.83% similarity) WP_099003921.1 (86.1% identity, 88.83%similarity) APG03758.0 55, 60, 65, 70, 75, 80, OUB31138.1 (61.62%identity, 66.6% 85, 90, 91, 92, 93, similarity) 94, 95, 96, 97, 98,WP_086396644.1 (60.3% identity, 63.96% 99, 100 similarity)WP_098838879.1 (48.54% identity, 57.4% similarity) WP_000358225.1(48.44% identity, 57.3% similarity) WP_098564358.1 (48.34% identity,57.3% similarity) EEM93103.1 (48.06% identity, 56.8% similarity)APG00572.0 US_2017_0175134_A1-46 (40.65% identity, 54.38% similarity)APG08081.0 (38.66% identity, 49.95% similarity) WP_087876328.1 (38.47%identity, 50.0% similarity) WP_000836979.1 (37.59% identity, 48.68%similarity) APG03778.0 60, 65, 70, 75, 80, 85, US_6555655_B1-14 (45.45%identity, 90, 91, 92, 93, 94, 56.33% similarity) 95, 96, 97, 98, 99,APG07310.0 (43.49% identity, 55.28% 100 similarity) US_6555655_B1-13(36.85% identity, 45.66% similarity) US_7692068_B2-2 (35.98% identity,53.32% similarity) WP_098826686.1 (35.05% identity, 48.04% similarity)APG00075.0 US_2016_0177333_A1-40 (34.87% identity, 47.79% similarity)APG06401.0 (33.57% identity, 44.94% similarity) APG08101.0 (33.04%identity, 46.19% similarity) APG07432.0 (32.73% identity, 47.09%similarity) APG00063.0 US_2016_0304898_A1-95 (32.43% identity, 47.64%similarity) APG03789.0 65, 70, 75, 80, 85, 90, APG00474.0US_2016_0366881_A1-89 91, 92, 93, 94, 95, (53.33% identity, 62.96%similarity) 96, 97, 98, 99, 100 APG00629.0 US_2016_0366881_A1-126(51.26% identity, 60.46% similarity) APG00050.0 US_2016_0304898_A1-80(46.8% identity, 57.08% similarity) WP_098902696.1 (46.8% identity,57.08% similarity) APG00557.0 US_2017_0175134_A1-36 (41.14% identity,55.23% similarity) APG00167.0 US_2016_0304898_A1-180 (39.06% identity,49.43% similarity) WP_098326411.1 (39.06% identity, 49.43% similarity)APG00461.0 US_2016_0311864_A1-182 (38.97% identity, 50.31% similarity)WP_098902378.1 (38.97% identity, 50.31% similarity) APG03964.0 (37.36%identity, 48.49% similarity) APG03826.0 50, 55, 60, 65, 70, 75,US_2015_0047076_A1-10 (28.2% identity, 80, 85, 90, 91, 92, 45.57%similarity) 93, 94, 95, 96, 97, US_2015_0047076_A1-4 (28.19% identity,98, 99, 100 46.98% similarity) APG03887.0 55, 60, 65, 70, 75, 80,WP_073006286.1 (37.3% identity, 54.34% 85, 90, 91, 92, 93, similarity)94, 95, 96, 97, 98, APG07780.0 (35.71% identity, 54.76% 99, 100similarity) APG01273.0 (35.15% identity, 54.27% similarity) APG08576.0(35.12% identity, 53.51% similarity) APG05984.0 (34.9% identity, 53.02%similarity) APG05943.0 (34.41% identity, 50.8% similarity) APG03587.0(32.66% identity, 49.83% similarity) APG09439.0 (32.44% identity, 49.5%similarity) APG01093.0 (32.32% identity, 51.52% similarity) APG04296.0(31.06% identity, 51.55% similarity) APG03893.0 93, 94, 95, 96, 97, 98,WP_012259841.1 (95.06% identity, 97.07% 99, 100 similarity)WP_088059022.1 (87.37% identity, 92.6% similarity) CA_2844913-24 (87.17%identity, 90.11% similarity) WP_088113460.1 (87.06% identity, 92.3%similarity) APG00800.0 US_2016_0355842_A1-172 (86.9% identity, 92.3%similarity) APG00643.0 US_2017_0175134_A1-68 (81.51% identity, 89.68%similarity) AST51780.1 (69.5% identity, 81.94% similarity) APG00088.0US_2016_0304898_A1-116 (66.62% identity, 76.54% similarity)WP_025988975.1 (62.92% identity, 66.41% similarity) WP_088610578.1(53.2% identity, 68.17% similarity) APG03909.0 55, 60, 65, 70, 75, 80,APG05279.0 (40.56% identity, 57.52% 85, 90, 91, 92, 93, similarity) 94,95, 96, 97, 98, APG03045.0 (33.55% identity, 49.19% 99, 100 similarity)APG02037.0 (30.61% identity, 51.48% similarity) APG01146.0 (30.5%identity, 50.26% similarity) APG05915.0 (29.76% identity, 44.23%similarity) WP_098783120.1 (29.66% identity, 45.08% similarity)CA_2844913-151 (26.06% identity, 44.63% similarity) CA_2844913-152(25.9% identity, 44.63% similarity) APG03959.0 99, 100 CA_2844913-98(94.91% identity, 98.15% similarity) WP_098524519.1 (94.44% identity,98.15% similarity) WP_098190289.1 (93.98% identity, 97.69% similarity)APG05662.0 (91.67% identity, 95.83% similarity) WP_076855475.1 (89.81%identity, 95.83% similarity) WP_088326113.1 (89.81% identity, 95.37%similarity) APG00394.0 US_2016_0366881_A1-48 (85.78% identity, 94.04%similarity) WP_097961786.1 (28.63% identity, 54.63% similarity)US_6093695_A-1 (27.35% identity, 54.27% similarity) WP_061667230.1(27.31% identity, 54.19% similarity) WP_098300708.1 (27.31% identity,54.19% similarity) WP_098315775.1 (27.31% identity, 54.19% similarity)APG04009.0 60, 65, 70, 75, 80, 85, APG05943.0 (43.61% identity, 57.32%90, 91, 92, 93, 94, similarity) 95, 96, 97, 98, 99, APG08576.0 (43.61%identity, 55.76% 100 similarity) APG05984.0 (42.77% identity, 55.97%similarity) APG07780.0 (42.5% identity, 55.94% similarity) APG03587.0(41.36% identity, 53.7% similarity) APG08917.0 (41.27% identity, 53.33%similarity) APG01273.0 (40.24% identity, 53.05% similarity) APG09439.0(39.88% identity, 53.27% similarity) APG01093.0 (39.81% identity, 53.29%similarity) APG05992.0 (39.17% identity, 53.18% similarity) APG04019.055, 60, 65, 70, 75, 80, WP_051505009.1 (68.82% identity, 75.29% 85, 90,91, 92, 93, similarity) 94, 95, 96, 97, 98, AKG37966.1 (56.7% identity,62.39% 99, 100 similarity) WP_052756038.1 (56.5% identity, 62.15%similarity) WP_087914274.1 (55.14% identity, 61.43% similarity)WP_042162506.1 (49.86% identity, 58.08% similarity) APG05946.0 (41.11%identity, 54.36% similarity) APG07202.0 (38.48% identity, 49.86%similarity) APG08348.0 (38.11% identity, 48.65% similarity)US_8829279_B2-4 (36.83% identity, 51.43% similarity) APG01809.0 (35.39%identity, 50.65% similarity) APG04103.0 80, 85, 90, 91, 92, 93,WP_051889583.1 (71.39% identity, 82.08% 94, 95, 96, 97, 98, similarity)99, 100 WP_051563179.1 (69.25% identity, 81.32% similarity)WP_051800214.1 (69.25% identity, 81.03% similarity) WP_051891081.1(68.97% identity, 81.32% similarity) WP_051889585.1 (68.97% identity,81.03% similarity) APG09110.0 (68.14% identity, 78.67% similarity)ACA38725.1 (66.94% identity, 78.61% similarity) APG03947.0 (66.94%identity, 78.61% similarity) APG05834.0 (61.99% identity, 75.15%similarity) APG02401.0 (55.33% identity, 70.61% similarity)WP_018673409.1 (55.33% identity, 70.61% similarity) WP_104030950.1(55.33% identity, 70.61% similarity) WP_104064947.1 (55.33% identity,70.61% similarity) APG04111.0 75, 80, 85, 90, 91, 92, APG04977.0 (63.92%identity, 74.23% 93, 94, 95, 96, 97, similarity) 98, 99, 100 APG02471.0(59.12% identity, 71.96% similarity) APG03738.0 (57.04% identity, 72.51%similarity) APG00236.0 US_2016_0355842_A1-32 (44.41% identity, 64.47%similarity) APG02633.0 (32.76% identity, 51.19% similarity) APG02714.0(31.21% identity, 50.34% similarity) APG00598.0 US_2016_0355842_A1-140(30.84% identity, 46.71% similarity) APG05793.0 (29.93% identity, 47.28%similarity) APG04141.0 98, 99, 100 APG09824.0 (94.19% identity, 97.44%similarity) APG03438.0 (93.95% identity, 96.98% similarity) APG09812.0(93.72% identity, 96.51% similarity) WP_103108858.1 (93.29% identity,96.06% similarity) KNB72291.1 (93.24% identity, 96.74% similarity)APG04458.0 (93.24% identity, 96.5% similarity) APG09936.0 (93.01%identity, 95.57% similarity) APG05978.0 (92.47% identity, 94.98%similarity) WP_084765780.1 (91.55% identity, 94.75% similarity)WP_093871939.1 (37.61% identity, 58.33% similarity) APG04162.0 70, 75,80, 85, 90, 91, WP_098228004.1 (100.0% identity, 100.0% 92, 93, 94, 95,96, similarity) 97, 98, 99, 100 WP_097984959.1 (51.66% identity, 64.65%similarity) WP_001267112.1 (51.36% identity, 64.05% similarity)JP_2016_516397-59 (51.31% identity, 66.18% similarity) ANS51604.1(50.73% identity, 65.6% similarity) US_8461415_B2-32 (50.59% identity,63.31% similarity) WP_078401252.1 (50.15% identity, 64.43% similarity)WP_023524027.1 (49.43% identity, 63.22% similarity) JP_2016_516397-60(49.27% identity, 64.14% similarity) APG02659.0 (49.01% identity, 63.1%similarity) APG04208.0 90, 91, 92, 93, 94, 95, WP_098412831.1 (91.83%identity, 94.8% 96, 97, 98, 99, 100 similarity) APG00369.0US_2016_0311864_A1-149 (83.18% identity, 85.87% similarity)WP_098858722.1 (78.4% identity, 85.92% similarity) APG00925.0US_2016_0311864_A1-227 (76.58% identity, 82.43% similarity) APG00282.0US_2016_0311864_A1-116 (74.51% identity, 81.07% similarity)WP_098386694.1 (72.06% identity, 81.37% similarity) WP_002191947.1(71.85% identity, 80.99% similarity) APG01063.0 (71.64% identity, 81.17%similarity) WP_002166959.1 (71.6% identity, 80.99% similarity)APG00308.0 US_2016_0311864_A1-135 (69.29% identity, 80.1% similarity)APG04302.0 90, 91, 92, 93, 94, 95, WP_098630027.1 (82.83% identity,87.19% 96, 97, 98, 99, 100 similarity) APG00499.0 US_2016_0311864_A1-185(82.56% identity, 87.19% similarity) APG07930.0 (74.66% identity, 85.01%similarity) APG00532.0 US_2016_0311864_A1-189 (70.7% identity, 81.72%similarity) WP_098821682.1 (70.7% identity, 81.72% similarity)APG03859.0 (63.49% identity, 77.38% similarity) WP_098818616.1 (61.14%identity, 73.83% similarity) WP_098838667.1 (60.62% identity, 73.58%similarity) CA_2844913-50 (60.36% identity, 73.58% similarity)APG00413.0 US_2016_0311864_A1-174 (50.65% identity, 63.19% similarity)APG04316.0 90, 91, 92, 93, 94, 95, WP_097969882.1 (100.0% identity,100.0% 96, 97, 98, 99, 100 similarity) WP_098173426.1 (99.68% identity,99.68% similarity) WP_098707157.1 (99.68% identity, 99.68% similarity)WP_098149421.1 (99.37% identity, 99.37% similarity) WP_098125701.1(94.95% identity, 97.16% similarity) WP_097837258.1 (94.32% identity,96.53% similarity) WP_098425855.1 (93.06% identity, 96.21% similarity)WP_098786706.1 (92.74% identity, 96.53% similarity) APG09415.0 (92.74%identity, 96.21% similarity) WP_070143005.1 (92.74% identity, 96.21%similarity) WP_098157467.1 (92.74% identity, 96.21% similarity)APG04323.0 80, 85, 90, 91, 92, 93, APG04375.0 (73.78% identity, 77.74%94, 95, 96, 97, 98, similarity) 99, 100 APG05300.0 (73.78% identity,77.44% similarity) WP_098222386.1 (73.78% identity, 75.91% similarity)APG04793.0 (73.17% identity, 77.13% similarity) WP_088080923.1 (73.17%identity, 75.91% similarity) WP_087969959.1 (72.87% identity, 75.61%similarity) APG03686.0 (72.56% identity, 77.44% similarity) APG03747.0(72.56% identity, 76.22% similarity) APG07676.0 (72.26% identity, 77.44%similarity) WP_098908549.1 (71.95% identity, 76.83% similarity)WP_098290465.1 (71.95% identity, 75.61% similarity) APG04360.0 98, 99,100 APG00017.0 US_2016_0304898_A1-28 (94.63% identity, 97.07%similarity) WP_098207009.1 (94.63% identity, 97.07% similarity)APG01309.0 (92.2% identity, 96.1% similarity) APG00464.0US_2016_0366881_A1-78 (90.73% identity, 95.61% similarity) APG01971.0(68.16% identity, 79.82% similarity) APG00194.0 US_2016_0311864_A1-65(55.16% identity, 70.4% similarity) OUB82185.1 (34.74% identity, 59.15%similarity) US_9121035_B2-20 (33.48% identity, 54.46% similarity)US_9121035_B2-6 (33.48% identity, 54.46% similarity) WP_088071709.1(33.04% identity, 56.25% similarity) APG04439.0 45, 50, 55, 60, 65, 70,US_8318900_B2-187 (25.06% identity, 75, 80, 85, 90, 91, 43.91%similarity) 92, 93, 94, 95, 96, US_8318900_B2-43 (25.06% identity, 97,98, 99, 100 43.91% similarity) US_8318900_B2-92 (25.06% identity, 43.91%similarity) WP_087986663.1 (25.06% identity, 43.68% similarity)APG04442.0 91, 92, 93, 94, 95, 96, APG06001.0 (84.83% identity, 90.45%97, 98, 99, 100 similarity) APG09232.0 (52.99% identity, 68.66%similarity) APG02168.0 (49.16% identity, 66.01% similarity) APG00441.0US_2016_0355842_A1-91 (38.68% identity, 56.16% similarity) APG00444.0US_2016_0355842_A1-93 (37.57% identity, 55.49% similarity) APG03867.0(37.57% identity, 52.6% similarity) APG00833.0 US_2016_0355842_A1-175(37.28% identity, 54.34% similarity) WP_098858716.1 (37.28% identity,54.34% similarity) APG04037.0 (36.31% identity, 52.16% similarity)APG03227.0 (35.92% identity, 50.86% similarity) APG04476.0 94, 95, 96,97, 98, 99, US_8461415_B2-14 (87.53% identity, 100 93.46% similarity)US_8461415_B2-42 (87.42% identity, 93.46% similarity) WP_097862021.1(87.42% identity, 93.35% similarity) WP_098329961.1 (87.42% identity,93.25% similarity) WP_098283907.1 (87.32% identity, 93.35% similarity)WP_098980505.1 (87.32% identity, 93.25% similarity) WP_097919094.1(87.22% identity, 93.25% similarity) WP_098213472.1 (87.12% identity,93.25% similarity) APG00096.0 US_2016_0304898_A1-125 (84.62% identity,90.84% similarity) WP_098720132.1 (84.52% identity, 90.73% similarity)APG04568.0 95, 96, 97, 98, 99, 100 APG08004.0 (91.73% identity, 94.74%similarity) APG05715.0 (90.98% identity, 94.74% similarity) APG06331.0(90.8% identity, 94.28% similarity) APG04176.0 (89.5% identity, 93.5%similarity) APG02445.0 (89.47% identity, 93.98% similarity)WP_098807694.1 (89.47% identity, 93.98% similarity) APG07911.0 (88.31%identity, 93.28% similarity) CA_2844913-146 (84.94% identity, 90.62%similarity) WP_070144216.1 (83.75% identity, 90.5% similarity)APG00212.0 US_2016_0311864_A1-75 (83.54% identity, 87.89% similarity)APG04602.0 70, 75, 80, 85, 90, 91, WP_098537347.1 (100.0% identity,100.0% 92, 93, 94, 95, 96, similarity) 97, 98, 99, 100 APG04931.0 (53.5%identity, 67.51% similarity) WP_098546457.1 (53.5% identity, 67.51%similarity) APG07526.0 (52.73% identity, 67.89% similarity)US_2016_0339078_A1-29674 (48.98% identity, 64.66% similarity) APG07790.0(48.67% identity, 65.23% similarity) WP_053512697.1 (48.6% identity,63.8% similarity) APG07577.0 (48.58% identity, 65.14% similarity)WP_088063470.1 (48.51% identity, 63.8% similarity) WP_048561796.1(48.14% identity, 63.71% similarity) APG04651.0 60, 65, 70, 75, 80, 85,APG03054.0 (61.03% identity, 74.92% 90, 91, 92, 93, 94, similarity) 95,96, 97, 98, 99, ANC11094.1 (60.95% identity, 75.8% 100 similarity)WP_080470008.1 (59.4% identity, 73.88% similarity) CBL59394.1 (43.89%identity, 60.82% similarity) CBL59393.1 (43.89% identity, 60.68%similarity) CBL59395.1 (43.89% identity, 60.68% similarity) CBL59396.1(43.89% identity, 60.68% similarity) CBL59397.1 (43.89% identity, 60.38%similarity) APG07954.0 (41.35% identity, 57.06% similarity) APG06921.0(41.12% identity, 59.76% similarity) APG04683.0 65, 70, 75, 80, 85, 90,WP_098487632.1 (90.46% identity, 90.46% 91, 92, 93, 94, 95, similarity)96, 97, 98, 99, 100 WP_098213667.1 (90.19% identity, 90.19% similarity)AGC39300.1 (61.4% identity, 71.76% similarity) WP_023524027.1 (57.58%identity, 66.58% similarity) APG02659.0 (51.41% identity, 62.21%similarity) APG02511.0 (50.67% identity, 67.12% similarity)US_8461415_B2-32 (50.0% identity, 62.63% similarity) WP_001267112.1(49.19% identity, 61.83% similarity) WP_078401252.1 (46.84% identity,61.05% similarity) JP_2016_516397-59 (46.21% identity, 59.79%similarity) APG04687.0 85, 90, 91, 92, 93, 94, APG01516.0 (72.39%identity, 84.45% 95, 96, 97, 98, 99, similarity) 100 APG06242.0 (71.54%identity, 84.57% similarity) US_8829279_B2-11 (64.4% identity, 76.36%similarity) APG06273.0 (61.2% identity, 74.04% similarity) APG09338.0(58.4% identity, 71.18% similarity) APG00051.0 US_2016_0304898_A1-81(57.75% identity, 70.25% similarity) WP_098425241.1 (57.75% identity,70.25% similarity) APG01858.0 (57.04% identity, 71.11% similarity)APG00911.0 US_2016_0355842_A1-189 (55.91% identity, 67.19% similarity)APG05896.0 (54.92% identity, 68.03% similarity) APG04692.0 60, 65, 70,75, 80, 85, WP_065486138.1 (37.01% identity, 53.81% 90, 91, 92, 93, 94,similarity) 95, 96, 97, 98, 99, WP_065487080.1 (37.01% identity, 53.81%100 similarity) APG00228.0 US_2016_0355842_A1-31 (36.2% identity, 56.25%similarity) WP_098139851.1 (34.62% identity, 51.28% similarity)US_8461415_B2-37 (34.53% identity, 51.08% similarity) APG00283.0US_2016_0355842_A1-50 (34.43% identity, 51.14% similarity) APG03623.0(34.04% identity, 49.87% similarity) APG00479.0 US_2016_0355842_A1-101(33.07% identity, 48.41% similarity) WP_098139684.1 (33.07% identity,48.41% similarity) WP_086387329.1 (32.56% identity, 46.98% similarity)APG04694.0 65, 70, 75, 80, 85, 90, CA_2844913-48 (50.51% identity, 62.5%91, 92, 93, 94, 95 similarity) 96, 97, 98, 99, 100 APG06942.0 (49.96%identity, 62.38% similarity) APG09805.0 (49.31% identity, 62.25%similarity) APG09805.0 (49.31% identity, 62.25% similarity)WP_087949412.1 (48.35% identity, 61.38% similarity) WP_098343937.1(47.4% identity, 58.72% similarity) APG00912.0 US_2017_0175134_A1-175(47.34% identity, 60.2% similarity) WP_078205753.1 (46.76% identity,59.73% similarity) WP_078205753.1 (46.76% identity, 59.73% similarity)WP_078205749.1 (46.54% identity, 58.25% similarity) APG04786.0 80, 85,90, 91, 92, 93, WP_002169796.1 (73.87% identity, 82.45% 94, 95, 96, 97,98, similarity) 99, 100 APG01013.0 (65.27% identity, 77.34% similarity)APG00044.0 US_2016_0304898_A1-71 (50.0% identity, 63.14% similarity)WP_098906559.1 (50.0% identity, 63.14% similarity) CA_2844913-189(48.13% identity, 50.75% similarity) CA_2844913-190 (47.89% identity,50.62% similarity) US_8461421_B2-264 (47.58% identity, 60.74%similarity) WP_088125814.1 (47.46% identity, 60.51% similarity)US_9567381_B2-378 (47.09% identity, 61.8% similarity) US_8461421_B2-140(46.46% identity, 59.28% similarity) APG04794.0 5, 10, 15, 20, 25, 30,WP_001063481.1 (87.86% identity, 90.17% 35, 40, 45, 50, 55, similarity)60, 65, 70, 75, 80, 85, WP_100240422.1 (30.75% identity, 50.57% 90, 91,92, 93, 94, similarity) 95, 96, 97, 98, 99, 100 APG04821.0 100APG00506.0 US_2016_0366881_A1-102 (98.33% identity, 99.0% similarity)APG00987.0 US_2016_0366881_A1-223 (93.65% identity, 95.99% similarity)APG02451.0 (91.3% identity, 94.98% similarity) US_8461415_B2-57 (49.51%identity, 68.4% similarity) APG00697.0 US_2016_0366881_A1-222 (45.9%identity, 66.89% similarity) APG00346.0 US_2016_0366881_A1-24 (45.25%identity, 66.56% similarity) US_8461415_B2-56 (43.38% identity, 67.22%similarity) APG01761.0 (41.32% identity, 62.46% similarity) APG00450.0US_2016_0366881_A1-72 (41.32% identity, 61.83% similarity) APG05804.0(40.76% identity, 61.15% similarity) APG04822.0 85, 90, 91, 92, 93, 94,APG00991.0 (81.44% identity, 83.25% 95, 96, 97, 98, 99, similarity) 100APG00055.0 US_2016_0304898_A1-86 (81.19% identity, 82.99% similarity)WP_098516321.1 (81.19% identity, 82.99% similarity) US_9328356_B2-17(70.9% identity, 76.84% similarity) US_9328356_B2-18 (70.9% identity,76.55% similarity) US_8796026_B2-6 (69.09% identity, 75.84% similarity)WP_061885068.1 (68.83% identity, 75.84% similarity) US_8796026_B2-8(68.52% identity, 76.72% similarity) US_9328356_B2-9 (68.29% identity,74.53% similarity) US_9328356_B2-10 (68.29% identity, 74.25% similarity)APG04959.0 60, 65, 70, 75, 80, 85, CA_2516349-9 (41.99% identity, 55.1%90, 91, 92, 93, 94, similarity) 95, 96, 97, 98, 99, APG04010.0 (41.74%identity, 54.82% 100 similarity) CA_2516349-8 (41.72% identity, 54.83%similarity) CA_2516349-7 (40.16% identity, 54.2% similarity) APG00726.0US_2017_0175134_A1-139 (37.93% identity, 50.62% similarity) APG07972.0(37.85% identity, 53.35% similarity) CA_2516349-11 (37.0% identity,49.47% similarity) US_9567381_B2-249 (36.97% identity, 52.8% similarity)US_2016_0345590_A1-2 (36.58% identity, 52.34% similarity) BAE79727.1(36.45% identity, 51.94% similarity) APG04966.0 93, 94, 95, 96, 97, 98,WP_002114997.1 (89.74% identity, 93.33% 99, 100 similarity)WP_070128649.1 (87.69% identity, 91.28% similarity) WP_098058849.1(87.44% identity, 93.33% similarity) WP_078212086.1 (86.92% identity,93.08% similarity) WP_097833300.1 (86.67% identity, 93.08% similarity)WP_098166690.1 (86.15% identity, 92.82% similarity) WP_098488891.1(86.15% identity, 92.56% similarity) WP_098665095.1 (85.9% identity,92.31% similarity) WP_001258160.1 (83.85% identity, 92.31% similarity)CA_2844913-10 (83.63% identity, 92.07% similarity) APG04977.0 85, 90,91, 92, 93, 94, APG03738.0 (74.45% identity, 86.13% 95, 96, 97, 98, 99,similarity) 100 APG02471.0 (72.1% identity, 81.52% similarity)APG04111.0 (63.92% identity, 74.23% similarity) APG00236.0US_2016_0355842_A1-32 (47.12% identity, 65.42% similarity) APG02633.0(33.33% identity, 50.88% similarity) APG02714.0 (30.46% identity, 49.01%similarity) APG05793.0 (27.43% identity, 46.18% similarity) APG05073.060, 65, 70, 75, 80, 85, APG02071.0 (45.78% identity, 58.49% 90, 91, 92,93, 94, similarity) 95, 96, 97, 98, 99, US_5281530-3 (45.63% identity,57.19% 100 similarity) CA_2595901-11 (45.59% identity, 57.16%similarity) CA_2595901-11 (45.59% identity, 57.16% similarity)US_5281530-4 (45.52% identity, 57.16% similarity) US_5281530-4 (45.52%identity, 57.16% similarity) US_9567381_B2-434 (45.4% identity, 55.37%similarity) US_9567381_B2-434 (45.4% identity, 55.37% similarity)WP_097983832.1 (45.33% identity, 55.09% similarity) WP_097983832.1(45.33% identity, 55.09% similarity) CA_2595901-12 (45.33% identity,55.02% similarity) CA_2595901-12 (45.33% identity, 55.02% similarity)APG05078.0 5, 10, 15, 20, 25, 30, APG01380.0 (96.52% identity, 98.26%35, 40, 45, 50, 55, similarity) 60, 65, 70, 75, 80, 85, WP_077106733.1(92.17% identity, 93.91% 90, 91, 92, 93, 94, similarity) 95, 96, 97, 98,99, WP_103583963.1 (92.17% identity, 93.91% 100 similarity) APG05095.060, 65, 70, 75, 80, 85, CA_2844913-178 (43.05% identity, 57.84% 90, 91,92, 93, 94, similarity) 95, 96, 97, 98, 99, APG00043.0US_2016_0304898_A1-69 100 (27.91% identity, 42.51% similarity)WP_098638318.1 (26.85% identity, 43.85% similarity) APG00446.0US_2016_0366881_A1-68 (26.47% identity, 43.57% similarity) APG00396.0US_2016_0311864_A1-163 (26.01% identity, 37.58% similarity)WP_098783217.1 (26.01% identity, 37.58% similarity) APG00250.0US_2016_0355842_A1-37 (25.79% identity, 41.5% similarity) WP_098376017.1(25.61% identity, 41.31% similarity) APG00053.0 US_2016_0304898_A1-83(25.42% identity, 41.31% similarity) WP_044306828.1 (25.42% identity,41.31% similarity) WP_097891105.1 (25.42% identity, 41.12% similarity)APG05115.0 90, 91, 92, 93, 94, 95, WO_2017_192560-379 (81.62% identity,96, 97, 98, 99, 100 88.34% similarity) APG02912.0 (78.9% identity, 86.0%similarity) WO_2017_192560-154 (78.26% identity, 86.76% similarity)WO_2017_192560-118 (77.67% identity, 86.17% similarity)WO_2017_192560-155 (77.47% identity, 86.17% similarity)WO_2017_192560-162 (77.47% identity, 85.77% similarity)WO_2017_192560-179 (77.12% identity, 86.19% similarity)WO_2017_192560-136 (77.12% identity, 85.8% similarity)WO_2017_192560-114 (77.12% identity, 85.4% similarity)WO_2017_192560-121 (77.08% identity, 85.57% similarity) APG05149.0 99,100 APG07049.0 (98.08% identity, 98.72% similarity) APG02921.0 (93.59%identity, 96.79% similarity) WP_097787431.1 (93.59% identity, 96.79%similarity) APG06989.0 (92.95% identity, 97.12% similarity)WP_098218026.1 (92.95% identity, 97.12% similarity) WP_098610169.1(92.63% identity, 97.44% similarity) APG08990.0 (91.67% identity, 96.15%similarity) WP_097849229.1 (91.03% identity, 94.23% similarity)APG01536.0 (90.71% identity, 94.55% similarity) APG04226.0 (89.42%identity, 93.27% similarity) APG05279.0 55, 60, 65, 70, 75, 80,APG03909.0 (40.56% identity, 57.52% 85, 90, 91, 92, 93, similarity) 94,95, 96, 97, 98, APG02037.0 (34.48% identity, 52.41% 99, 100 similarity)APG01146.0 (34.03% identity, 52.26% similarity) APG03045.0 (33.22%identity, 50.16% similarity) APG05915.0 (33.17% identity, 47.97%similarity) WP_098783120.1 (29.84% identity, 45.9% similarity)CA_2844913-152 (25.67% identity, 41.42% similarity) CA_2844913-151(25.51% identity, 41.42% similarity) APG00615.0 US_2016_0366881_A1-119(25.16% identity, 37.66% similarity) APG05300.0 96, 97, 98, 99, 100APG04375.0 (92.25% identity, 94.83% similarity) WP_098222386.1 (91.7%identity, 95.47% similarity) APG04793.0 (91.39% identity, 94.76%similarity) WP_087969959.1 (91.32% identity, 95.09% similarity)APG07676.0 (90.64% identity, 95.51% similarity) WP_098908549.1 (90.57%identity, 95.47% similarity) WP_098162508.1 (90.19% identity, 95.09%similarity) APG03686.0 (89.89% identity, 94.76% similarity)WP_088080923.1 (89.43% identity, 93.96% similarity) WP_098290465.1(88.68% identity, 94.34% similarity) APG05346.0 96, 97, 98, 99, 100WP_098111055.1 (93.39% identity, 96.56% similarity) CA_2844913-120(92.59% identity, 95.5% similarity) APG00202.0 US_2016_0304898_A1-195(89.59% identity, 92.64% similarity) APG02475.0 (88.04% identity, 92.62%similarity) APG04431.0 (85.75% identity, 90.84% similarity) APG00208.0US_2016_0304898_A1-201 (85.06% identity, 89.37% similarity) APG01443.0(84.72% identity, 91.19% similarity) APG07094.0 (81.58% identity, 89.47%similarity) APG00352.0 US_2016_0366881_A1-29 (81.58% identity, 89.21%similarity) WP_000715637.1 (75.79% identity, 84.74% similarity)APG05388.0 96, 97, 98, 99, 100 US_7214788_B2-2 (90.67% identity, 95.52%similarity) AQM56933.1 (88.06% identity, 94.03% similarity)US_9328356_B2-30 (86.57% identity, 93.28% similarity) CA_2267665-3(75.75% identity, 84.33% similarity) US_7214788_B2-10 (61.36% identity,64.65% similarity) US_7214788_B2-6 (61.36% identity, 64.65% similarity)US_7214788_B2-33 (60.75% identity, 64.0% similarity) US_7214788_B2-8(60.75% identity, 64.0% similarity) US_7214788_B2-14 (60.5% identity,63.75% similarity) US_7214788_B2-16 (51.38% identity, 56.89% similarity)APG05408.0 55, 60, 65, 70, 75, 80, WP_098933161.1 (99.33% identity,100.0% 85, 90, 91, 92, 93, similarity) 94, 95, 96, 97, 98, APG03204.0(38.86% identity, 50.86% 99, 100 similarity) APG05414.0 80, 85, 90, 91,92, 93, US_8461415_B2-37 (73.86% identity, 94, 95, 96, 97, 98, 77.41%similarity) 99, 100 US_8461421_B2-79 (59.2% identity, 68.16% similarity)WP_086387329.1 (58.93% identity, 69.13% similarity) WP_065486138.1(36.27% identity, 50.93% similarity) WP_065487080.1 (36.0% identity,50.67% similarity) APG00228.0 US_2016_0355842_A1-31 (35.65% identity,45.93% similarity) APG00966.0 US_2016_0355842_A1-195 (35.34% identity,52.05% similarity) WP_098976737.1 (35.34% identity, 52.05% similarity)APG00438.0 US_2016_0366881_A1-63 (33.66% identity, 48.51% similarity)WP_078401253.1 (33.53% identity, 51.63% similarity) APG05429.0 93, 94,95, 96, 97, 98, WP_078404344.1 (96.87% identity, 97.65% 99, 100similarity) OPA24196.1 (93.99% identity, 94.26% similarity) APG04807.0(91.12% identity, 92.43% similarity) APG08307.0 (48.98% identity, 62.76%similarity) APG09864.0 (31.66% identity, 48.29% similarity) APG02256.0(30.62% identity, 47.85% similarity) APG07058.0 (30.46% identity, 47.0%similarity) WP_078404291.1 (30.46% identity, 47.0% similarity)APG03055.0 (25.12% identity, 43.35% similarity) APG05431.0 92, 93, 94,95, 96, 97, WP_098882546.1 (93.11% identity, 95.08% 98, 99, 100similarity) WP_098814109.1 (85.62% identity, 93.79% similarity)APG08085.0 (84.74% identity, 91.56% similarity) WP_098090730.1 (84.74%identity, 91.56% similarity) APG01155.0 (84.42% identity, 91.56%similarity) APG00743.0 (84.04% identity, 91.86% similarity)WP_098096847.1 (84.04% identity, 91.86% similarity) WP_098813802.1(84.04% identity, 91.21% similarity) WP_098643289.1 (83.99% identity,92.48% similarity) WP_097849502.1 (83.71% identity, 91.21% similarity)APG05471.0 45, 50, 55, 60, 65, 70, WP_090462870.1 (44.86% identity,57.01% 75, 80, 85, 90, 91, similarity) 92, 93, 94, 95, 96,WP_090462867.1 (44.38% identity, 58.75% 97, 98, 99, 100 similarity)APG08370.0 (42.52% identity, 57.3% similarity) WP_076377347.1 (41.65%identity, 55.83% similarity) WP_076375462.1 (33.07% identity, 49.61%similarity) WP_091163039.1 (32.29% identity, 46.06% similarity)WP_078670461.1 (32.16% identity, 47.13% similarity) SKA29206.1 (31.61%identity, 46.4% similarity) WP_100340242.1 (31.35% identity, 46.94%similarity) WP_062550478.1 (29.63% identity, 47.59% similarity)APG05481.0 94, 95, 96, 97, 98, 99, APG05341.0 (86.91% identity, 93.19%100 similarity) APG00324.0 US_2016_0355842_A1-61 (71.5% identity, 80.83%similarity) APG01281.0 (61.44% identity, 70.69% similarity) APG00253.0US_2016_0355842_A1-39 (47.51% identity, 60.89% similarity) APG00020.0US_2016_0304898_A1-33 (46.46% identity, 60.37% similarity)WP_098539891.1 (46.46% identity, 60.37% similarity) APG00322.0US_2016_0355842_A1-59 (46.37% identity, 62.18% similarity) APG01508.0(46.19% identity, 60.37% similarity) WP_098322759.1 (46.19% identity,60.37% similarity) APG00764.0 US_2016_0355842_A1-168 (46.13% identity,61.86% similarity) APG05492.0 95, 96, 97, 98, 99, 100 WP_065397681.1(99.64% identity, 100.0% similarity) APG03604.0 (88.53% identity, 94.62%similarity) WP_089734181.1 (86.74% identity, 93.91% similarity)WP_086572501.1 (38.31% identity, 56.17% similarity) WP_100573349.1(37.54% identity, 52.55% similarity) WP_067347886.1 (36.39% identity,50.0% similarity) WP_053730552.1 (36.04% identity, 53.45% similarity)WP_039633689.1 (34.97% identity, 49.13% similarity) WP_071966410.1(34.71% identity, 51.47% similarity) WP_102926521.1 (34.68% identity,49.13% similarity) APG05508.0 90, 91, 92, 93, 94, 95, WP_098018669.1(76.34% identity, 86.88% 96, 97, 98, 99, 100 similarity) APG00030.0US_2016_0304898_A1-50 (76.29% identity, 86.8% similarity) APG01313.0(74.9% identity, 86.26% similarity) US_8461415_B2-14 (74.67% identity,84.86% similarity) US_8461415_B2-42 (74.56% identity, 84.86% similarity)WP_097862021.1 (74.56% identity, 84.76% similarity) WP_098329961.1(74.56% identity, 84.76% similarity) WP_098283907.1 (74.46% identity,84.76% similarity) WP_098980505.1 (74.46% identity, 84.65% similarity)WP_097919094.1 (74.46% identity, 84.55% similarity) APG05538.0 80, 85,90, 91, 92, 93, APG09282.0 (67.52% identity, 76.85% 94, 95, 96, 97, 98,similarity) 99, 100 APG02533.0 (63.01% identity, 73.67% similarity)APG00787.0 US_2017_0175134_A1-156 (60.49% identity, 67.13% similarity)APG00471.0 US_2016_0366881_A1-85 (58.19% identity, 71.29% similarity)APG01860.0 (57.65% identity, 63.19% similarity) APG09630.0 (50.52%identity, 60.86% similarity) KXH80331.1 (49.93% identity, 57.96%similarity) WP_098395829.1 (49.24% identity, 60.49% similarity)WP_082770008.1 (48.81% identity, 56.64% similarity) APG05689.0 (47.8%identity, 59.91% similarity) APG05636.0 100 APG02554.0 (97.98% identity,99.33% similarity) APG09682.0 (96.3% identity, 98.65% similarity)APG00201.0 US_2016_0304898_A1-193 (81.25% identity, 87.83% similarity)WP_098866878.1 (81.25% identity, 87.83% similarity) APG00847.0US_2016_0304898_A1-223 (80.92% identity, 87.83% similarity) APG07639.0(80.81% identity, 88.55% similarity) APG03379.0 (80.54% identity, 88.26%similarity) APG08241.0 (80.47% identity, 88.55% similarity) APG02279.0(80.07% identity, 87.25% similarity) WP_102956800.1 (79.93% identity,86.84% similarity) APG05643.0 75, 80, 85, 90, 91, 92, US_9567381_B2-447(63.64% identity, 93, 94, 95, 96, 97, 70.1% similarity) 98, 99, 100APG00123.0 US_2016_0304898_A1-146 (45.83% identity, 57.61% similarity)WP_098213628.1 (45.83% identity, 57.61% similarity) APG00136.0US_2016_0177333_A1-75 (26.34% identity, 38.35% similarity)WP_097984025.1 (26.34% identity, 38.35% similarity) APG00721.0US_2017_0175134_A1-133 (26.08% identity, 38.36% similarity) APG05662.097, 98, 99, 100 WP_076855475.1 (93.06% identity, 97.69% similarity)WP_088326113.1 (93.06% identity, 97.22% similarity) WP_098190289.1(92.59% identity, 97.22% similarity) CA_2844913-98 (92.59% identity,96.76% similarity) WP_098524519.1 (92.13% identity, 96.76% similarity)APG03959.0 (91.67% identity, 95.83% similarity) APG00394.0US_2016_0366881_A1-48 (85.32% identity, 92.66% similarity)WP_097961786.1 (29.86% identity, 55.66% similarity) WP_063538656.1(29.41% identity, 54.75% similarity) WP_061667230.1 (28.51% identity,55.2% similarity) WP_098300708.1 (28.51% identity, 55.2% similarity)APG05725.0 55, 60, 65, 70, 75, 80, CA_2844913-184 (47.48% identity,54.7% 85, 90, 91, 92, 93, similarity) 94, 95, 96, 97, 98, APG09826.0(38.39% identity, 52.98% 99, 100 similarity) APG06774.0 (37.79%identity, 49.01% similarity) US_8461415_B2-45 (35.36% identity, 47.49%similarity) WP_097891157.1 (35.36% identity, 47.49% similarity)APG03573.0 (35.23% identity, 48.01% similarity) WP_098349415.1 (34.84%identity, 47.14% similarity) APG04280.0 (34.76% identity, 52.23%similarity) APG00424.0 US_2016_0366881_A1-52 (34.61% identity, 45.74%similarity) WP_060852738.1 (34.61% identity, 45.74% similarity)APG05734.0 94, 95, 96, 97, 98, 99, WP_097832796.1 (89.41% identity,92.66% 100 similarity) ETT82181.1 (89.29% identity, 93.02% similarity)WP_098519790.1 (89.29% identity, 92.54% similarity) US_9567381_B2-455(88.93% identity, 93.02% similarity) WP_016093954.1 (88.81% identity,92.54% similarity) WP_033729515.1 (88.81% identity, 92.54% similarity)APG01446.0 (88.74% identity, 92.57% similarity) WP_002147758.1 (88.45%identity, 92.54% similarity) AGU12794.1 (88.45% identity, 92.3%similarity) APG00705.0 US_2017_0175134_A1-120 (88.04% identity, 92.51%similarity) APG05793.0 75, 80, 85, 90, 91, 92, APG02714.0 (83.68%identity, 84.03% 93, 94, 95, 96, 97, similarity) 98, 99, 100 APG06768.0(62.76% identity, 73.1% similarity) APG02633.0 (37.1% identity, 54.42%similarity) APG04111.0 (29.93% identity, 47.28% similarity) APG04977.0(27.43% identity, 46.18% similarity) APG00236.0 US_2016_0355842_A1-32(26.6% identity, 47.47% similarity) APG06578.0 (25.96% identity, 37.82%similarity) APG05834.0 75, 80, 85, 90, 91, 92, APG04103.0 (61.99%identity, 75.15% 93, 94, 95, 96, 97 similarity) 98, 99, 100 APG00585.0US_2016_0366881_A1-116 (60.41% identity, 73.61% similarity)WP_104030950.1 (60.41% identity, 73.31% similarity) WP_099327316.1(60.41% identity, 72.73% similarity) WP_104150403.1 (60.18% identity,71.09% similarity) WP_094701865.1 (60.12% identity, 73.61% similarity)APG02401.0 (60.12% identity, 73.31% similarity) WP_018673409.1 (60.12%identity, 73.31% similarity) WP_104064947.1 (60.12% identity, 73.31%similarity) APG06560.0 (59.59% identity, 71.39% similarity) APG00851.0US_2016_0311864_A1-222 (59.59% identity, 70.5% similarity)WP_061663532.1 (59.59% identity, 70.5% similarity) APG05878.0 85, 90,91, 92, 93, 94, APG00603.0 US_2016_0311864_A1-198 95, 96, 97, 98, 99,(69.75% identity, 83.74% similarity) 100 WP_097981477.1 (69.75%identity, 83.74% similarity) WP_098887934.1 (69.55% identity, 83.54%similarity) APG01084.0 (67.28% identity, 84.16% similarity) APG08526.0(67.28% identity, 83.13% similarity) WP_098867980.1 (66.87% identity,80.86% similarity) WP_098505564.1 (56.17% identity, 68.93% similarity)APG01112.0 US_2017_0175134_A1-204 (55.14% identity, 68.93% similarity)WP_097891153.1 (33.4% identity, 49.9% similarity) WP_044306756.1 (33.2%identity, 49.71% similarity) WP_098353196.1 (33.2% identity, 49.51%similarity) APG05907.0 90, 91, 92, 93, 94, 95, APG01153.0 (82.93%identity, 88.29% 96, 97, 98, 99, 100 similarity) APG00095.0US_2016_0177333_A1-52 (68.28% identity, 77.24% similarity) APG00700.0US_2016_0311864_A1-207 (68.04% identity, 77.48% similarity) APG00750.0US_2016_0366881_A1-132 (32.76% identity, 47.54% similarity) APG00629.0US_2016_0366881_A1-126 (31.72% identity, 46.9% similarity) APG07432.0(30.41% identity, 42.88% similarity) APG08101.0 (29.9% identity, 44.27%similarity) APG00474.0 US_2016_0366881_A1-89 (29.23% identity, 42.23%similarity) APG06401.0 (29.16% identity, 44.15% similarity)US_9567381_B2-398 (28.28% identity, 40.61% similarity) APG05917.0 94,95, 96, 97, 98, 99, APG09153.0 (88.67% identity, 93.35% 100 similarity)APG08195.0 (82.6% identity, 88.97% similarity) APG07868.0 (82.35%identity, 87.99% similarity) APG00727.0 US_2016_0311864_A1-210 (81.46%identity, 87.07% similarity) APG00178.0 US_2016_0311864_A1-54 (81.37%identity, 87.75% similarity) APG06237.0 (81.13% identity, 89.46%similarity) APG04627.0 (80.64% identity, 87.5% similarity) APG00315.0US_2016_0311864_A1-137 (77.45% identity, 85.29% similarity) APG06559.0(76.04% identity, 85.57% similarity) WP_098771878.1 (35.47% identity,40.89% similarity) APG05920.0 45, 50, 55, 60, 65, 70, WP_098396645.1(76.71% identity, 85.1% 75, 80, 85, 90, 91, similarity) 92, 93, 94, 95,96, APG06420.0 (29.91% identity, 43.59% 97, 98, 99, 100 similarity)APG00239.0 US_2016_0311864_A1-91 (28.75% identity, 42.35% similarity)APG01803.0 (27.44% identity, 38.72% similarity) APG00375.0US_2016_0311864_A1-152 (26.36% identity, 38.1% similarity)WP_098805991.1 (26.36% identity, 38.1% similarity) APG00564.0US_2016_0355842_A1-128 (26.15% identity, 40.83% similarity)US_8318900_B2-91 (25.54% identity, 36.62% similarity) APG05946.0 75, 80,85, 90, 91, 92, US_8829279_B2-4 (64.61% identity, 73.7% 93, 94, 95, 96,97, similarity) 98, 99, 100 WP_098689320.1 (58.54% identity, 66.4%similarity) WP_098478562.1 (57.99% identity, 65.58% similarity)APG00231.0 US_2016_0311864_A1-86 (57.91% identity, 65.68% similarity)APG00568.0 US_2016_0311864_A1-391 (57.91% identity, 65.68% similarity)WP_050845516.1 (57.72% identity, 66.12% similarity) APG00377.0US_2016_0311864_A1-154 (57.64% identity, 65.68% similarity) AEX56523.1(57.45% identity, 66.12% similarity) WP_098830366.1 (57.45% identity,65.31% similarity) WP_098639761.1 (57.45% identity, 65.04% similarity)APG05955.0 60, 65, 70, 75, 80, 85, APG03361.0 (64.06% identity, 75.59%90, 91, 92, 93, 94, similarity) 95, 96, 97, 98, 99, APG09146.0 (39.73%identity, 55.19% 100 similarity) US_8461415_B2-29 (39.43% identity,53.21% similarity) WP_016099737.1 (36.46% identity, 50.54% similarity)US_8461415_B2-44 (32.17% identity, 45.77% similarity) APG06401.0 (29.44%identity, 43.09% similarity) APG07310.0 (29.09% identity, 39.5%similarity) WP_098826686.1 (29.0% identity, 43.95% similarity)APG00075.0 US_2016_0177333_A1-40 (28.85% identity, 43.72% similarity)APG08101.0 (28.67% identity, 42.83% similarity) APG05992.0 91, 92, 93,94, 95, 96, APG08917.0 (81.62% identity, 90.81% 97, 98, 99, 100similarity) APG01706.0 (61.01% identity, 75.45% similarity) APG05984.0(59.78% identity, 73.55% similarity) APG07780.0 (58.27% identity, 71.94%similarity) APG08576.0 (57.76% identity, 71.12% similarity) APG05943.0(56.46% identity, 69.39% similarity) APG01093.0 (55.6% identity, 70.76%similarity) APG09439.0 (52.88% identity, 69.06% similarity) APG03587.0(51.97% identity, 68.1% similarity) APG01273.0 (51.94% identity, 70.67%similarity) APG06027.0 90, 91, 92, 93, 94, 95, APG07511.0 (81.32%identity, 88.95% 96, 97, 98, 99, 100 similarity) WP_098772949.1 (80.78%identity, 87.53% similarity) APG00230.0 US_2016_0311864_A1-85 (80.57%identity, 87.31% similarity) APG00413.0 US_2016_0311864_A1-174 (77.89%identity, 86.32% similarity) APG06920.0 (77.14% identity, 87.27%similarity) KEZ80012.1 (77.11% identity, 86.05% similarity) APG05717.0(76.58% identity, 85.79% similarity) US_9321814_B2-3 (76.58% identity,84.74% similarity) US_9321814_B2-2 (76.38% identity, 84.51% similarity)WP_098876539.1 (76.32% identity, 84.47% similarity) APG06049.0 91, 92,93, 94, 95, 96, APG06319.0 (84.2% identity, 91.01% 97, 98, 99, 100similarity) APG00483.0 US_2016_0355842_A1-104 (84.2% identity, 90.19%similarity) APG06851.0 (80.38% identity, 90.74% similarity) APG00421.0US_2016_0355842_A1-86 (79.29% identity, 89.37% similarity)WP_063226258.1 (76.16% identity, 87.95% similarity) APG00995.0US_2016_0355842_A1-200 (69.38% identity, 80.49% similarity) APG00414.0US_2016_0355842_A1-81 (68.29% identity, 80.22% similarity) APG00593.0US_2016_0355842_A1-136 (68.29% identity, 79.95% similarity)WP_097899326.1 (68.29% identity, 79.95% similarity) APG00560.0US_2016_0355842_A1-125 (65.85% identity, 79.4% similarity)WP_098991345.1 (65.85% identity, 79.4% similarity) APG06079.0 94, 95,96, 97, 98, 99, APG00041.0 US_2016_0304898_A1-64 100 (90.21% identity,93.43% similarity) WP_098583123.1 (90.21% identity, 93.43% similarity)APG00145.0 US_2016_0304898_A1-163 (86.98% identity, 89.93% similarity)WP_098772031.1 (86.98% identity, 89.93% similarity) AEH76822.1 (65.14%identity, 75.0% similarity) US_8461421_B2-84 (64.76% identity, 74.55%similarity) EJR94916.1 (64.5% identity, 76.25% similarity)WP_080029306.1 (64.11% identity, 75.79% similarity) AQM56942.1 (60.59%identity, 67.0% similarity) US_8461421_B2-184 (60.12% identity, 67.07%similarity) APG06195.0 98, 99, 100 APG06465.0 (96.39% identity, 97.38%similarity) APG04483.0 (96.39% identity, 97.05% similarity)WP_098908548.1 (96.39% identity, 97.05% similarity) APG00696.0US_2016_0355842_A1-160 (96.07% identity, 97.05% similarity)WP_098222384.1 (96.07% identity, 97.05% similarity) WP_098778568.1(96.07% identity, 97.05% similarity) WP_098162507.1 (96.07% identity,96.72% similarity) WP_088080922.1 (95.41% identity, 97.05% similarity)APG01882.0 (95.1% identity, 96.41% similarity) WP_098290464.1 (95.1%identity, 96.41% similarity) APG01265.0 (95.08% identity, 97.05%similarity) APG02105.0 (95.08% identity, 96.07% similarity) APG08138.0(93.77% identity, 96.07% similarity) APG03715.0 (91.56% identity, 92.5%similarity) WP_087969960.1 (90.6% identity, 92.16% similarity)APG06213.0 90, 91, 92, 93, 94, 95, APG06606.0 (96.84% identity, 98.59%96, 97, 98, 99, 100 similarity) APG02110.0 (95.78% identity, 97.89%similarity) APG07811.0 (95.08% identity, 97.19% similarity) APG04108.0(83.28% identity, 87.46% similarity) APG00235.0 US_2016_0311864_A1-89(81.77% identity, 88.37% similarity) WP_050845493.1 (64.63% identity,75.34% similarity) APG08681.0 (54.79% identity, 65.93% similarity)WP_048536324.1 (54.79% identity, 65.93% similarity) APG00702.0US_2017_0175134_A1-115 (38.1% identity, 50.79% similarity) APG00702.0US_2017_0175134_A1-115 (38.1% identity, 50.79% similarity) APG06235.096, 97, 98, 99, 100 APG00181.0 US_2016_0355842_A1-16 (92.25% identity,95.32% similarity) APG00038.0 US_2016_0355842_A1-1 (89.95% identity,93.9% similarity) APG09221.0 (89.75% identity, 93.53% similarity)APG00675.0 US_2017_0175134_A1-97 (61.35% identity, 75.53% similarity)APG00131.0 US_2016_0311864_A1-30 (60.53% identity, 76.28% similarity)WP_098631820.1 (60.53% identity, 76.28% similarity) APG07054.0 (51.4%identity, 67.83% similarity) APG00207.0 US_2016_0311864_A1-70 (46.38%identity, 64.31% similarity) WP_098685831.1 (46.38% identity, 64.31%similarity) AAY27873.1 (36.7% identity, 55.77% similarity) APG06283.080, 85, 90, 91, 92, 93, WP_098069633.1 (66.67% identity, 79.33% 94, 95,96, 97, 98, similarity) 99, 100 WP_098099018.1 (66.58% identity, 79.18%similarity) WP_098087707.1 (66.58% identity, 78.92% similarity)WP_099010057.1 (66.32% identity, 78.92% similarity) APG01797.0 (65.89%identity, 78.81% similarity) APG03217.0 (65.57% identity, 77.97%similarity) APG08029.0 (65.14% identity, 77.86% similarity) APG04119.0(64.25% identity, 78.24% similarity) APG00932.0 (61.42% identity, 76.4%similarity) APG02206.0 (61.19% identity, 75.12% similarity) APG06304.075, 80, 85, 90, 91, 92, APG07609.0 (66.46% identity, 70.19% 93, 94, 95,96, 97, similarity) 98, 99, 100 APG08693.0 (54.63% identity, 73.15%similarity) OUB07579.1 (54.63% identity, 73.15% similarity) APG01808.0(46.3% identity, 61.64% similarity) APG00505.0 US_2016_0366881_A1-101(42.51% identity, 58.68% similarity) OUB07573.1 (42.51% identity, 58.68%similarity) WP_098652068.1 (41.81% identity, 59.36% similarity)APG00858.0 (41.69% identity, 58.91% similarity) APG03543.0 (41.62%identity, 58.98% similarity) US_8318900_B2-75 (41.52% identity, 59.06%similarity) WP_098179785.1 (41.52% identity, 59.06% similarity)APG06319.0 96, 97, 98, 99, 100 APG00483.0 US_2016_0355842_A1-104 (91.53%identity, 95.08% similarity) APG00421.0 US_2016_0355842_A1-86 (86.89%identity, 94.54% similarity) APG06851.0 (86.61% identity, 95.08%similarity) APG06049.0 (84.2% identity, 91.01% similarity)WP_063226258.1 (74.93% identity, 84.47% similarity) APG00995.0US_2016_0355842_A1-200 (69.35% identity, 81.18% similarity) APG00593.0US_2016_0355842_A1-136 (68.55% identity, 80.91% similarity)WP_097899326.1 (68.55% identity, 80.91% similarity) APG00414.0US_2016_0355842_A1-81 (68.46% identity, 80.86% similarity) APG04455.0(65.42% identity, 76.94% similarity) APG06331.0 97, 98, 99, 100APG07911.0 (93.03% identity, 96.02% similarity) APG04176.0 (91.04%identity, 93.53% similarity) APG04568.0 (90.8% identity, 94.28%similarity) APG05715.0 (90.55% identity, 93.03% similarity)CA_2844913-146 (89.43% identity, 93.12% similarity) APG02445.0 (89.05%identity, 92.54% similarity) WP_098807694.1 (89.05% identity, 92.54%similarity) APG08004.0 (89.05% identity, 92.29% similarity)WP_070144216.1 (85.82% identity, 91.04% similarity) APG09027.0 (85.32%identity, 91.79% similarity) APG06350.0 5, 10, 15, 20, 25, 30,CEL21287.1 (61.85% identity, 74.11% 35, 40, 45, 50, 55, similarity) 60,65, 70, 75, 80, 85, WP_042194387.1 (59.67% identity, 70.84% 90, 91, 92,93, 94, similarity) 95, 96, 97, 98, 99, WP_083472203.1 (57.82% identity,68.17% 100 similarity) ALG11962.1 (57.8% identity, 68.01% similarity)WP_083752681.1 (57.03% identity, 68.17% similarity) ONI90142.1 (56.6%identity, 67.39% similarity) WP_042191061.1 (55.91% identity, 67.72%similarity) AHH99199.1 (52.72% identity, 65.76% similarity)WP_037915008.1 (47.17% identity, 62.26% similarity) WP_081789577.1(44.03% identity, 54.65% similarity) APG06378.0 80, 85, 90, 91, 92, 93,US_2016_0339078_A1-28394 (68.57% 94, 95, 96, 97, 98, identity, 77.14%similarity) 99, 100 US_2016_0339078_A1-28394 (68.57% identity, 77.14%similarity) US_2016_0339078_A1-28394 (68.57% identity, 77.14%similarity) EOO61631.1 (62.08% identity, 68.05% similarity) EOO61631.1(62.08% identity, 68.05% similarity) EOO61631.1 (62.08% identity, 68.05%similarity) EOO61631.1 (62.08% identity, 68.05% similarity) EJR89704.1(40.84% identity, 48.67% similarity) EJR89704.1 (40.84% identity, 48.67%similarity) EJR89704.1 (40.84% identity, 48.67% similarity) EJR89704.1(40.84% identity, 48.67% similarity) EJR89704.1 (40.84% identity, 48.67%similarity) EJR89704.1 (40.84% identity, 48.67% similarity) EJR89704.1(40.84% identity, 48.67% similarity) APG06424.0 65, 70, 75, 80, 85, 90,APG08171.0 (92.19% identity, 96.21% 91, 92, 93, 94, 95, similarity) 96,97, 98, 99, 100 APG09835.0 (91.76% identity, 94.88% similarity)APG08468.0 (91.31% identity, 95.1% similarity) APG08852.0 (90.42%identity, 94.43% similarity) OPA11477.1 (90.42% identity, 94.43%similarity) APG00184.0 US_2016_0355842_A1-19 (59.82% identity, 64.51%similarity) WP_044306772.1 (59.82% identity, 64.51% similarity)WP_098816555.1 (59.6% identity, 64.29% similarity) WP_097891175.1(59.38% identity, 64.29% similarity) WP_098217793.1 (59.38% identity,64.29% similarity) APG06479.0 99, 100 APG09726.0 (97.2% identity, 98.6%similarity) APG02659.0 (50.13% identity, 59.79% similarity) APG02511.0(47.37% identity, 61.32% similarity) JP_2016_516397-59 (46.67% identity,56.0% similarity) ANS51604.1 (46.13% identity, 55.47% similarity)PGY60284.1 (45.94% identity, 46.22% similarity) WP_078401252.1 (45.73%identity, 61.71% similarity) WP_098213667.1 (45.16% identity, 58.06%similarity) WP_098487632.1 (45.16% identity, 58.06% similarity)JP_2016_516397-60 (45.07% identity, 54.4% similarity) APG06480.0 85, 90,91, 92, 93, 94, APG00718.0 US_2017_0175134_A1-132 95, 96, 97, 98, 99,(82.72% identity, 83.57% similarity) 100 APG00860.0US_2016_0355842_A1-180 (46.67% identity, 62.78% similarity)US_8318900_B2-77 (36.74% identity, 53.59% similarity) APG04021.0 (36.46%identity, 51.74% similarity) APG04586.0 (36.39% identity, 52.5%similarity) APG04750.0 (35.81% identity, 53.58% similarity) APG00586.0US_2016_0355842_A1-135 (35.71% identity, 52.91% similarity) APG09100.0(35.71% identity, 51.06% similarity) APG03324.0 (35.54% identity, 53.85%similarity) APG08768.0 (35.22% identity, 52.42% similarity) APG06493.097, 98, 99, 100 APG00142.0 US_2016_0304898_A1-162 (94.83% identity,96.33% similarity) APG06496.0 65, 70, 75, 80, 85, 90, US_9567381_B2-1427(40.39% identity, 91, 92, 93, 94, 95, 60.78% similarity) 96, 97, 98, 99,100 US_9567381_B2-1429 (40.39% identity, 60.78% similarity)WO_2017_199078-15 (40.39% identity, 60.78% similarity) APG09181.0(40.08% identity, 63.97% similarity) ABV22504.1 (40.0% identity, 60.39%similarity) AFA52539.1 (40.0% identity, 60.39% similarity) AGT15589.1(40.0% identity, 60.39% similarity) US_9567381_B2-522 (40.0% identity,60.39% similarity) WO_2017_199078-16 (40.0% identity, 60.39% similarity)WO_2017_199078-18 (40.0% identity, 60.39% similarity) WO_2017_199078-4(40.0% identity, 60.39% similarity) WO_2017_199078-6 (40.0% identity,60.39% similarity) WP_043938803.1 (40.0% identity, 60.39% similarity)APG06559.0 94, 95, 96, 97, 98, 99, APG00315.0 US_2016_0311864_A1-137 100(88.45% identity, 91.89% similarity) APG07868.0 (87.22% identity, 92.14%similarity) APG08195.0 (86.98% identity, 93.12% similarity) APG04627.0(86.49% identity, 92.87% similarity) APG00178.0 US_2016_0311864_A1-54(85.96% identity, 91.87% similarity) APG06237.0 (85.5% identity, 92.63%similarity) APG00727.0 US_2016_0311864_A1-210 (84.03% identity, 90.42%similarity) APG05917.0 (76.04% identity, 85.57% similarity) APG09153.0(74.02% identity, 84.56% similarity) WP_098771878.1 (34.98% identity,40.64% similarity) APG06576.0 80, 85, 90, 91, 92, 93, APG00089.0US_2016_0304898_A1-118 94, 95, 96, 97, 98, (70.02% identity, 79.34%similarity) 99, 100 WP_098905641.1 (70.02% identity, 79.34% similarity)US_9695440_B2-8 (53.26% identity, 63.58% similarity) US_9695440_B2-9(53.16% identity, 63.29% similarity) US_9695440_B2-6 (53.14% identity,63.69% similarity) US_9567381_B2-327 (51.75% identity, 64.11%similarity) JP_2002_335967-2 (50.59% identity, 62.69% similarity)US_8461421_B2-99 (50.11% identity, 61.85% similarity) US_8759619_B2-25(49.62% identity, 60.53% similarity) WP_098722307.1 (49.62% identity,60.53% similarity) WP_098733730.1 (49.62% identity, 60.53% similarity)APG06578.0 55, 60, 65, 70, 75, 80, APG02166.0 (44.26% identity, 60.98%85, 90, 91, 92, 93, similarity) 94, 95, 96, 97, 98, APG09957.0 (39.63%identity, 53.25% 99, 100 similarity) APG02633.0 (34.83% identity, 52.07%similarity) APG06768.0 (31.58% identity, 46.75% similarity) APG00236.0US_2016_0355842_A1-32 (31.21% identity, 49.04% similarity) APG02714.0(29.69% identity, 44.06% similarity) APG05793.0 (25.96% identity, 37.82%similarity) APG06606.0 90, 91, 92, 93, 94, 95, APG06213.0 (96.84%identity, 98.59% 96, 97, 98, 99, 100 similarity) APG02110.0 (95.08%identity, 97.19% similarity) APG07811.0 (95.08% identity, 96.84%similarity) APG04108.0 (81.36% identity, 86.59% similarity) APG00235.0US_2016_0311864_A1-89 (80.9% identity, 87.85% similarity) WP_050845493.1(63.44% identity, 75.51% similarity) APG08681.0 (54.39% identity, 65.36%similarity) WP_048536324.1 (54.39% identity, 65.36% similarity)APG00702.0 US_2017_0175134_A1-115 (37.52% identity, 50.56% similarity)APG00702.0 US_2017_0175134_A1-115 (37.52% identity, 50.56% similarity)APG06677.0 80, 85, 90, 91, 92, 93, WP_061663292.1 (89.04% identity,89.61% 94, 95, 96, 97, 98, similarity) 99, 100 CA_2516349-42 (72.6%identity, 76.09% similarity) CA_2682227-77 (72.6% identity, 76.09%similarity) CA_2682227-78 (72.6% identity, 76.09% similarity)US_5424410_A-29 (72.51% identity, 76.09% similarity) EP_2728007-44(63.31% identity, 70.25% similarity) US_9567381_B2-472 (61.92% identity,69.37% similarity) US_9447153_B1-404 (61.87% identity, 71.21%similarity) US_9447153_B1-377 (61.18% identity, 70.43% similarity)US_9447153_B1-375 (61.13% identity, 70.36% similarity) APG06697.0 90,91, 92, 93, 94, 95, WP_098063843.1 (90.3% identity, 90.3% 96, 97, 98,99, 100 similarity) WP_000839920.1 (90.07% identity, 90.07% similarity)WP_097861094.1 (89.84% identity, 90.07% similarity) APG00336.0US_2016_0366881_A1-19 (78.64% identity, 85.23% similarity) APG00262.0US_2016_0311864_A1-101 (76.8% identity, 83.33% similarity) APG00223.0US_2016_0311864_A1-82 (75.86% identity, 85.06% similarity) APG00242.0US_2016_0311864_A1-93 (75.63% identity, 84.83% similarity) APG00454.0US_2016_0311864_A1-179 (75.17% identity, 84.83% similarity) APG00335.0US_2016_0366881_A1-18 (74.71% identity, 84.37% similarity) APG00118.0US_2016_0311864_A1-28 (74.31% identity, 84.17% similarity) APG06705.091, 92, 93, 94, 95, 96, APG05711.0 (86.6% identity, 90.41% 97, 98, 99,100 similarity) APG00182.0 US_2016_0355842_A1-17 (67.84% identity,76.86% similarity) CUB55361.1 (35.14% identity, 51.21% similarity)WP_078401802.1 (34.54% identity, 48.38% similarity) APG08067.0 (27.42%identity, 41.79% similarity) APG05519.0 (26.71% identity, 41.28%similarity) APG00097.0 US_2016_0177333_A1-53 (25.91% identity, 39.25%similarity) APG00048.0 US_2016_0177333_A1-22 (25.64% identity, 38.25%similarity) APG00950.0 US_2016_0355842_A1-192 (25.57% identity, 41.06%similarity) APG00101.0 US_2016_0177333_A1-57 (25.47% identity, 41.16%similarity) APG06718.0 50, 55, 60, 65, 70, 75, APG06496.0 (33.84%identity, 50.57% 80, 85, 90, 91, 92, similarity) 93, 94, 95, 96, 97,APG09181.0 (28.85% identity, 49.62% 98, 99, 100 similarity)US_9567381_B2-1430 (26.97% identity, 48.69% similarity) WP_088071735.1(26.97% identity, 48.69% similarity) APG06738.0 65, 70, 75, 80, 85, 90,JP_2003_284568-4 (51.82% identity, 91, 92, 93, 94, 95, 64.24%similarity) 96, 97, 98, 99, 100 US_8461421_B2-103 (50.3% identity,63.69% similarity) ANN35811.1 (49.09% identity, 62.12% similarity)2D42_A (45.45% identity, 56.06% similarity) US_2015_0047076_A1-6 (31.52%identity, 49.09% similarity) US_2015_0047076_A1-8 (30.9% identity,46.94% similarity) US_2015_0047076_A1-10 (30.21% identity, 45.16%similarity) US_2015_0047076_A1-2 (30.0% identity, 45.76% similarity)US_2015_0047076_A1-4 (29.85% identity, 45.97% similarity)US_9567381_B2-384 (29.82% identity, 46.08% similarity) APG06774.0 55,60, 65, 70, 75, 80, APG05725.0 (37.79% identity, 49.01% 85, 90, 91, 92,93, similarity) 94, 95, 96, 97, 98, WP_017762581.1 (35.62% identity,50.64% 99, 100 similarity) APG05278.0 (35.58% identity, 53.96%similarity) APG05402.0 (35.5% identity, 53.17% similarity) APG03741.0(35.26% identity, 50.32% similarity) APG04175.0 (35.23% identity, 50.0%similarity) WP_044306759.1 (35.2% identity, 51.97% similarity)WP_098394433.1 (35.2% identity, 51.97% similarity) US_8461415_B2-45(35.2% identity, 51.81% similarity) WP_097891157.1 (35.03% identity,51.81% similarity) APG06791.0 75, 80, 85, 90, 91, 92, APG00594.0US_2016_0355842_A1-137 93, 94, 95, 96, 97, (55.49% identity, 70.33%similarity) 98, 99, 100 WP_097795410.1 (55.49% identity, 70.33%similarity) APG00154.0 US_2016_0355842_A1-5 (55.22% identity, 70.33%similarity) WP_090920591.1 (54.67% identity, 70.33% similarity)APG06049.0 (54.45% identity, 67.28% similarity) APG00483.0US_2016_0355842_A1-104 (53.97% identity, 67.46% similarity) APG06319.0(53.79% identity, 67.1% similarity) APG00486.0 US_2016_0355842_A1-107(53.3% identity, 69.23% similarity) APG00560.0 US_2016_0355842_A1-125(52.79% identity, 66.84% similarity) WP_098991345.1 (52.79% identity,66.84% similarity) APG06883.0 70, 75, 80, 85, 90, 91, APG00471.0US_2016_0366881_A1-85 92, 93, 94, 95, 96, (63.28% identity, 69.55%similarity) 97, 98, 99, 100 APG00232.0 US_2016_0311864_A1-87 (51.22%identity, 61.84% similarity) WP_098806888.1 (51.22% identity, 61.84%similarity) APG02533.0 (49.02% identity, 60.15% similarity) APG00787.0US_2017_0175134_A1-156 (48.79% identity, 63.77% similarity) APG09282.0(45.2% identity, 60.37% similarity) KXH80331.1 (44.54% identity, 57.85%similarity) WP_098395829.1 (44.46% identity, 60.15% similarity)APG07719.0 (44.26% identity, 58.97% similarity) APG07057.0 (44.09%identity, 52.38% similarity) APG06898.0 90, 91, 92, 93, 94, 95,WP_017154552.1 (78.74% identity, 86.61% 96, 97, 98, 99, 100 similarity)APG00596.0 US_2016_0311864_A1-196 (75.85% identity, 85.3% similarity)APG06027.0 (73.49% identity, 83.73% similarity) APG00741.0US_2016_0311864_A1-213 (70.42% identity, 81.41% similarity) APG00649.0US_2017_0175134_A1-75 (70.05% identity, 80.73% similarity) APG06920.0(69.55% identity, 81.1% similarity) KEZ80012.1 (69.55% identity, 79.79%similarity) APG07511.0 (69.03% identity, 79.53% similarity) APG03279.0(68.77% identity, 80.05% similarity) APG05717.0 (68.77% identity, 79.79%similarity) APG06937.0 90, 91, 92, 93, 94, 95, APG00128.0US_2016_0304898_A1-157 96, 97, 98, 99, 100 (83.74% identity, 86.99%similarity) WP_098207006.1 (83.74% identity, 86.99% similarity)WP_016110459.1 (83.33% identity, 86.99% similarity) APG02923.0 (82.93%identity, 86.59% similarity) APG06385.0 (81.53% identity, 84.74%similarity) WP_098682674.1 (66.4% identity, 79.76% similarity)APG00126.0 US_2016_0304898_A1-153 (65.99% identity, 79.35% similarity)WP_098207007.1 (65.99% identity, 79.35% similarity) APG00437.0US_2016_0366881_A1-62 (63.04% identity, 75.49% similarity)WP_016110460.1 (60.96% identity, 76.1% similarity) APG06954.0 5, 10, 15,20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93,94, 95, 96, 97, 98, 99, 100 APG07054.0 75, 80, 85, 90, 91, 92,APG00675.0 US_2017_0175134_A1-97 93, 94, 95, 96, 97, (53.04% identity,70.09% similarity) 98, 99, 100 APG00131.0 US_2016_0311864_A1-30 (52.53%identity, 69.11% similarity) WP_098631820.1 (52.53% identity, 69.11%similarity) APG06235.0 (51.4% identity, 67.83% similarity) APG00038.0US_2016_0355842_A1-1 (50.87% identity, 68.36% similarity) APG00181.0US_2016_0355842_A1-16 (50.87% identity, 67.94% similarity) APG09221.0(50.61% identity, 67.54% similarity) APG00207.0 US_2016_0311864_A1-70(46.94% identity, 63.61% similarity) WP_098685831.1 (46.94% identity,63.61% similarity) AAY27873.1 (38.77% identity, 56.17% similarity)APG07057.0 65, 70, 75, 80, 85, 90, APG00471.0 US_2016_0366881_A1-85 91,92, 93, 94, 95, (54.65% identity, 62.68% similarity) 96, 97, 98, 99, 100APG06883.0 (44.09% identity, 52.38% similarity) APG00232.0US_2016_0311864_A1-87 (43.02% identity, 51.8% similarity) WP_098806888.1(43.02% identity, 51.8% similarity) APG05538.0 (41.96% identity, 53.79%similarity) APG09282.0 (39.22% identity, 50.85% similarity) APG02533.0(36.91% identity, 49.17% similarity) WP_061139797.1 (34.26% identity,47.15% similarity) APG07719.0 (32.8% identity, 44.69% similarity)APG00457.0 US_2016_0355842_A1-98 (31.98% identity, 44.62% similarity)WP_098376005.1 (31.98% identity, 44.62% similarity) APG07094.0 98, 99,100 APG00352.0 US_2016_0366881_A1-29 (95.26% identity, 97.89%similarity) WP_098111055.1 (82.63% identity, 89.47% similarity)CA_2844913-120 (81.58% identity, 89.74% similarity) APG05346.0 (81.58%identity, 89.47% similarity) APG00202.0 US_2016_0304898_A1-195 (79.8%identity, 86.11% similarity) APG00208.0 US_2016_0304898_A1-201 (79.04%identity, 86.36% similarity) APG02475.0 (76.96% identity, 86.84%similarity) APG01443.0 (75.77% identity, 86.86% similarity) APG04431.0(74.68% identity, 85.06% similarity) WP_000715637.1 (74.02% identity,83.2% similarity) APG07107.0 85, 90, 91, 92, 93, 94, WP_065212006.1(91.1% identity, 95.55% 95, 96, 97, 98, 99, similarity) 100 APG00633.0US_2016_0355842 A1-148 (73.67% identity, 84.02% similarity)WP_098866818.1 (73.67% identity, 84.02% similarity) APG00552.0US_2016_0366881_A1-110 (58.28% identity, 71.6% similarity)WP_061885189.1 (53.25% identity, 68.05% similarity) WP_088118891.1(53.25% identity, 68.05% similarity) APG08369.0 (51.6% identity, 68.22%similarity) WP_016078640.1 (50.89% identity, 71.01% similarity)APG00541.0 US_2016_0355842_A1-119 (49.7% identity, 66.86% similarity)WP_097855808.1 (49.7% identity, 66.86% similarity) WP_098581475.1 (49.7%identity, 66.86% similarity) APG07137.0 85, 90, 91, 92, 93, 94,WP_100797528.1 (79.62% identity, 87.58% 95, 96, 97, 98, 99, similarity)100 WP_036122586.1 (70.57% identity, 79.43% similarity) KUF37510.1(70.57% identity, 79.11% similarity) WP_010859436.1 (70.45% identity,77.31% similarity) APG00010.0 US_2016_0177333_A1-8 (70.43% identity,80.18% similarity) WP_082673669.1 (70.43% identity, 80.18% similarity)APG00489.0 US_2016_0177333_A1-123 (70.25% identity, 80.67% similarity)APG00350.0 US_2016_0366881_A1-27 (65.61% identity, 78.66% similarity)US_8318900_B2-74 (64.97% identity, 77.71% similarity) US_8318900_B2-25(64.65% identity, 77.71% similarity) APG07162.0 99, 100 APG00062.0US_2016_0177333_A1-29 (97.28% identity, 98.34% similarity)WP_074651312.1 (93.8% identity, 97.28% similarity) APG02438.0 (68.32%identity, 79.27% similarity) WP_098349012.1 (60.54% identity, 74.4%similarity) WP_098515340.1 (60.39% identity, 74.4% similarity)WP_098675605.1 (60.39% identity, 74.4% similarity) WP_097920476.1(60.24% identity, 74.4% similarity) WP_098563827.1 (60.24% identity,74.4% similarity) WP_098236459.1 (60.09% identity, 74.25% similarity)WP_064532221.1 (59.94% identity, 73.95% similarity) APG07176.0 55, 60,65, 70, 75, 80, APG08495.0 (39.66% identity, 61.36% 85, 90, 91, 92, 93,similarity) 94, 95, 96, 97, 98, APG07983.0 (33.9% identity, 52.4% 99,100 similarity) APG02088.0 (31.43% identity, 51.43% similarity)APG09675.0 (31.25% identity, 50.94% similarity) APG07732.0 (29.56%identity, 46.23% similarity) APG02633.0 (28.04% identity, 50.68%similarity) APG07249.0 60, 65, 70, 75, 80, 85, APG01163.0 (60.92%identity, 73.85% 90, 91, 92, 93, 94, similarity) 95, 96, 97, 98, 99,APG00580.0 US_2016_0355842_A1-132 100 (43.63% identity, 59.77%similarity) WP_098146720.1 (43.63% identity, 59.77% similarity)US_8461415_B2-38 (42.41% identity, 54.71% similarity) APG00560.0US_2016_0355842_A1-125 (41.45% identity, 56.48% similarity)WP_098991345.1 (41.45% identity, 56.48% similarity) APG00269.0US_2016_0355842_A1-46 (41.28% identity, 56.15% similarity)WP_097904769.1 (41.28% identity, 56.15% similarity) APG00414.0US_2016_0355842_A1-81 (41.19% identity, 56.22% similarity) APG00995.0US_2016_0355842_A1-200 (41.19% identity, 56.22% similarity) APG07257.055, 60, 65, 70, 75, 80, WP_082180198.1 (44.47% identity, 49.69% 85, 90,91, 92, 93, similarity) 94, 95, 96, 97, 98, EJQ98132.1 (41.01% identity,42.71% 99, 100 similarity) APG00315.0 US_2016_0311864_A1-137 (35.85%identity, 51.15% similarity) APG09153.0 (34.44% identity, 49.59%similarity) APG04627.0 (34.3% identity, 49.17% similarity) APG05917.0(34.23% identity, 48.76% similarity) APG00178.0 US_2016_0311864_A1-54(34.17% identity, 50.0% similarity) APG06237.0 (33.95% identity, 49.48%similarity) APG08195.0 (33.88% identity, 49.79% similarity) APG00727.0US_2016_0311864_A1-210 (32.66% identity, 47.46% similarity) APG07307.05, 10, 15, 20, 25, 30, WP_057916440.1 (73.89% identity, 73.89% 35, 40,45, 50, 55, similarity) 60, 65, 70, 75, 80, 85, WP_057972564.1 (72.22%identity, 72.78% 90, 91, 92, 93, 94, similarity) 95, 96, 97, 98, 99,WP_051884290.1 (55.0% identity, 62.22% 100 similarity) ALN65243.1(31.67% identity, 32.78% similarity) APG07310.0 60, 65, 70, 75, 80, 85,APG03778.0 (43.49% identity, 55.28% 90, 91, 92, 93, 94, similarity) 95,96, 97, 98, 99, US_6555655_B1-14 (43.32% identity, 100 57.58%similarity) APG06401.0 (35.2% identity, 47.11% similarity)US_6555655_B1-13 (35.12% identity, 46.68% similarity) US_7692068_B2-2(34.24% identity, 49.18% similarity) WP_098826686.1 (34.14% identity,47.14% similarity) APG00075.0 US_2016_0177333_A1-40 (33.97% identity,46.9% similarity) APG00063.0 US_2016_0304898_A1-95 (33.15% identity,46.89% similarity) APG00706.0 US_2017_0175134_A1-126 (32.44% identity,48.21% similarity) APG00750.0 US_2016_0366881_A1-132 (32.34% identity,47.21% similarity) APG07318.0 85, 90, 91, 92, 93, 94, APG04365.0 (74.78%identity, 82.68% 95, 96, 97, 98, 99, similarity) 100 CA_2992084-2(47.35% identity, 58.94% similarity) APG09293.0 (44.82% identity, 57.77%similarity) WP_087876282.1 (44.53% identity, 57.54% similarity)WP_088113050.1 (44.38% identity, 57.21% similarity)US_2016_0339078_A1-28464 (44.19% identity, 56.9% similarity)US_2016_0339078_A1-29674 (43.82% identity, 58.14% similarity) APG09984.0(43.8% identity, 56.5% similarity) WP_098779744.1 (43.8% identity, 56.5%similarity) APG04069.0 (43.76% identity, 55.25% similarity) APG07322.085, 90, 91, 92, 93, 94, APG08378.0 (100.0% identity, 100.0% 95, 96, 97,98, 99, similarity) 100 CA_2516349-51 (76.93% identity, 84.67%similarity) US_9567381_B2-373 (69.57% identity, 78.73% similarity)US_8044266_B2-3 (67.7% identity, 77.43% similarity) CA_2516349-14(67.56% identity, 77.43% similarity) CA_2516349-16 (67.28% identity,77.01% similarity) US_9567381_B2-374 (66.03% identity, 75.78%similarity) APG00204.0 US_2016_0177333_A1-89 (65.14% identity, 75.04%similarity) WP_098223478.1 (64.84% identity, 74.74% similarity)APG00695.0 US_2017_0175134_A1-109 (63.2% identity, 74.48% similarity)APG07432.0 90, 91, 92, 93, 94, 95, APG08101.0 (79.52% identity, 86.08%96, 97, 98, 99, 100 similarity) APG00075.0 US_2016_0177333_A1-40 (57.23%identity, 71.29% similarity) WP_098826686.1 (56.64% identity, 70.7%similarity) APG06401.0 (52.48% identity, 64.16% similarity)US_7692068_B2-2 (42.17% identity, 55.32% similarity) APG00750.0US_2016_0366881_A1-132 (40.47% identity, 55.01% similarity) APG00063.0US_2016_0304898_A1-95 (37.92% identity, 53.09% similarity)US_9567381_B2-394 (37.5% identity, 51.56% similarity) APG00734.0US_2016_0366881_A1-131 (36.8% identity, 49.63% similarity)US_9567381_B2-398 (36.65% identity, 52.05% similarity) APG07463.0 55,60, 65, 70, 75, 80, WP_073006288.1 (44.25% identity, 65.16% 85, 90, 91,92, 93, similarity) 94, 95, 96, 97, 98, APG08577.0 (36.49% identity,54.04% 99, 100 similarity) WP_073006284.1 (35.92% identity, 59.86%similarity) SHH89794.1 (35.91% identity, 56.04% similarity)WP_073008904.1 (33.99% identity, 51.82% similarity) WP_084723762.1(33.44% identity, 52.68% similarity) APG04978.0 (32.62% identity, 52.48%similarity) APG02115.0 (31.4% identity, 52.22% similarity) APG07467.099, 100 APG01949.0 (95.71% identity, 97.85% similarity) APG05337.0(95.09% identity, 98.47% similarity) WP_050845726.1 (95.09% identity,98.47% similarity) WP_000823322.1 (94.79% identity, 98.16% similarity)WP_086397429.1 (94.17% identity, 98.16% similarity) APG07936.0 (93.87%identity, 96.63% similarity) APG02293.0 (93.56% identity, 98.47%similarity) WP_098222113.1 (93.56% identity, 98.47% similarity)WP_016084062.1 (92.64% identity, 97.24% similarity) APG04806.0 (92.64%identity, 96.63% similarity) APG04251.0 (92.33% identity, 96.93%similarity) WP_016085044.1 (92.33% identity, 96.93% similarity)WP_016084449.1 (92.33% identity, 96.63% similarity) WP_016085279.1(91.41% identity, 95.71% similarity) WP_016084436.1 (90.8% identity,95.4% similarity) WP_016083901.1 (90.49% identity, 95.71% similarity)APG07487.0 91, 92, 93, 94, 95, 96, APG00224.0 US_2016_0355842_A1-28 97,98, 99, 100 (86.65% identity, 90.91% similarity) APG03760.0 (73.46%identity, 79.89% similarity) WP_098584415.1 (73.46% identity, 79.89%similarity) APG08240.0 (72.73% identity, 80.4% similarity) APG05634.0(61.19% identity, 73.09% similarity) WP_098775686.1 (61.19% identity,73.09% similarity) APG00609.0 US_2017_0175134_A1-57 (60.62% identity,71.39% similarity) US_8829279_B2-37 (59.33% identity, 72.98% similarity)US_8318900_B2-63 (59.17% identity, 72.5% similarity) APG05506.0 (56.79%identity, 71.47% similarity) APG07511.0 96, 97, 98, 99, 100 APG05717.0(89.87% identity, 95.47% similarity) APG09069.0 (87.73% identity, 93.33%similarity) APG00230.0 US_2016_0311864_A1-85 (86.24% identity, 92.33%similarity) WP_098772949.1 (86.24% identity, 92.06% similarity)APG00413.0 US_2016_0311864_A1-174 (82.71% identity, 91.22% similarity)APG06920.0 (82.28% identity, 89.95% similarity) US_9321814_B2-2 (81.96%identity, 89.39% similarity) US_9321814_B2-3 (81.7% identity, 89.12%similarity) WP_098876539.1 (81.43% identity, 88.86% similarity)APG06027.0 (81.32% identity, 88.95% similarity) APG07564.0 91, 92, 93,94, 95, 96, APG00083.0 US_2016_0304898_A1-111 97, 98, 99, 100 (88.38%identity, 90.12% similarity) APG00083.0 US_2016_0304898_A1-111 (88.38%identity, 90.12% similarity) WP_098213625.1 (88.38% identity, 90.12%similarity) WP_098213625.1 (88.38% identity, 90.12% similarity)APG02071.0 (43.16% identity, 57.62% similarity) APG02071.0 (43.16%identity, 57.62% similarity) CA_2595901-13 (39.69% identity, 54.27%similarity) CA_2595901-13 (39.69% identity, 54.27% similarity)WP_103655243.1 (39.48% identity, 54.88% similarity)US_2011_0239334_A1-80 (39.31% identity, 55.93% similarity) APG07609.055, 60, 65, 70, 75, 80, APG06304.0 (66.46% identity, 70.19% 85, 90, 91,92, 93, similarity) 94, 95, 96, 97, 98, APG08693.0 (41.38% identity,54.55% 99, 100 similarity) OUB07579.1 (41.38% identity, 54.55%similarity) APG01808.0 (34.82% identity, 45.13% similarity) OUB27300.1(30.86% identity, 43.62% similarity) APG00364.0 US_2016_0366881_A1-35(30.56% identity, 43.62% similarity) WP_098652068.1 (29.29% identity,43.79% similarity) US_8318900_B2-75 (28.99% identity, 43.49% similarity)WP_098179785.1 (28.99% identity, 43.49% similarity) APG04781.0 (28.0%identity, 42.29% similarity) APG07636.0 75, 80, 85, 90, 91, 92,APG01288.0 (60.31% identity, 70.57% 93, 94, 95, 96, 97, similarity) 98,99, 100 WP_098214356.1 (60.31% identity, 70.57% similarity)US_2011_0239334_A1-84 (58.53% identity, 65.54% similarity)US_2011_0239334_A1-82 (58.14% identity, 65.14% similarity) APG00801.0US_2017_0175134_A1-160 (55.3% identity, 67.06% similarity) APG00099.0US_2016_0177333_A1-55 (54.73% identity, 66.31% similarity)WP_098487817.1 (54.73% identity, 66.31% similarity) APG00784.0US_2017_0175134_A1-148 (54.35% identity, 65.82% similarity)US_2012_0110706_A1-22 (52.74% identity, 59.52% similarity) APG02221.0(52.3% identity, 66.81% similarity) APG07664.0 60, 65, 70, 75, 80, 85,WP_081083112.1 (49.19% identity, 66.94% 90, 91, 92, 93, 94 similarity)95, 96, 97, 98, 99, WP_081056378.1 (49.19% identity, 66.13% 100similarity) WP_085040093.1 (46.77% identity, 59.68% similarity)WP_034193354.1 (45.97% identity, 59.68% similarity) WP_089445918.1(45.97% identity, 57.26% similarity) APG07643.0 (45.6% identity, 59.2%similarity) WP_015876201.1 (45.16% identity, 61.29% similarity)WP_059574313.1 (45.16% identity, 58.06% similarity) WP_060965848.1(45.16% identity, 58.06% similarity) WP_035977960.1 (41.48% identity,57.04% similarity) APG07665.0 85, 90, 91, 92, 93, 94, US_7919272_B2-23(77.86% identity, 95, 96, 97, 98, 99, 84.33% similarity) 100WP_103591151.1 (77.46% identity, 83.85% similarity) US_7919272_B2-13(75.89% identity, 81.78% similarity) CA_2753918-13 (75.71% identity,81.59% similarity) WP_103591619.1 (75.5% identity, 81.3% similarity)APG00786.0 US_2017_0175134_A1-153 (71.34% identity, 77.7% similarity)APG04827.0 (69.45% identity, 76.77% similarity) APG00662.0US_2017_0175134_A1-84 (67.72% identity, 75.89% similarity) APG00079.0US_2016_0177333_A1-43 (67.2% identity, 76.04% similarity) WP_016084057.1(64.74% identity, 73.6% similarity) WP_016084447.1 (64.74% identity,73.6% similarity) WP_016085042.1 (64.74% identity, 73.6% similarity)APG07732.0 65, 70, 75, 80, 85, 90, APG02592.0 (45.49% identity, 66.32%91, 92, 93, 94, 95, similarity) 96, 97, 98, 99, 100 APG05918.0 (45.12%identity, 60.27% similarity) APG02088.0 (44.94% identity, 61.08%similarity) APG09675.0 (43.23% identity, 60.32% similarity) APG07824.0(42.63% identity, 61.13% similarity) APG09455.0 (40.69% identity, 54.89%similarity) APG06880.0 (40.06% identity, 55.05% similarity) APG00598.0US_2016_0355842_A1-140 (39.24% identity, 58.54% similarity) APG08495.0(33.97% identity, 47.62% similarity) APG02633.0 (31.85% identity, 53.08%similarity) APG07753.0 70, 75, 80, 85, 90, 91, WP_072335847.1 (66.37%identity, 77.98% 92, 93, 94, 95, 96, similarity) 97, 98, 99, 100APG09460.0 (50.0% identity, 66.47% similarity) APG00416.0US_2016_0355842_A1-82 (47.6% identity, 64.97% similarity) WP_098425282.1(47.6% identity, 64.97% similarity) APG05956.0 (47.31% identity, 65.27%similarity) APG00793.0 US_2016_0355842_A1-170 (47.04% identity, 64.23%similarity) APG07606.0 (44.5% identity, 59.52% similarity) APG05239.0(42.03% identity, 59.13% similarity) APG02970.0 (42.03% identity, 57.68%similarity) APG01367.0 (41.83% identity, 60.46% similarity) APG07798.05, 10, 15, 20, 25, 30, OPA04743.1 (100.0% identity, 100.0% 35, 40, 45,50, 55, similarity) 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96,97, 98, 99, 100 APG07810.0 65, 70, 75, 80, 85, 90, WO_2014_102697-1(44.53% identity, 91, 92, 93, 94, 95, 60.73% similarity) 96, 97, 98, 99,100 WO_2014_102697-2 (44.03% identity, 59.86% similarity)WO_2014_102697-60 (44.03% identity, 59.86% similarity) WO_2014_102697-3(43.37% identity, 59.2% similarity) WO_2014_102697-61 (43.37% identity,59.2% similarity) WP_016098322.1 (42.48% identity, 56.52% similarity)APG00543.0 US_2017_0175134_A1-31 (41.61% identity, 56.49% similarity)APG08642.0 (40.15% identity, 53.45% similarity) AGV55018.1 (39.64%identity, 53.53% similarity) US_9567381_B2-246 (38.79% identity, 51.6%similarity) APG07811.0 90, 91, 92, 93, 94, 95, APG02110.0 (95.61%identity, 97.54% 96, 97, 98, 99, 100 similarity) APG06213.0 (95.08%identity, 97.19% similarity) APG06606.0 (95.08% identity, 96.84%similarity) APG00235.0 US_2016_0311864_A1-89 (83.51% identity, 88.89%similarity) APG04108.0 (82.93% identity, 87.63% similarity)WP_050845493.1 (64.12% identity, 75.51% similarity) APG08681.0 (55.96%identity, 66.14% similarity) WP_048536324.1 (55.96% identity, 66.14%similarity) APG00702.0 US_2017_0175134_A1-115 (38.89% identity, 51.75%similarity) APG00401.0 US_2016_0311864_A1-168 (33.93% identity, 44.69%similarity) APG00401.0 US_2016_0311864_A1-168 (33.93% identity, 44.69%similarity) APG07945.0 80, 85, 90, 91, 92, 93, US_2016_0122399_A1-2(64.49% identity, 94, 95, 96, 97, 98, 74.9% similarity) 99, 100US_2016_0122399_A1-1 (64.41% identity, 74.83% similarity)US_8318900_B2-81 (64.22% identity, 75.36% similarity)US_2016_0122399_A1-4 (64.18% identity, 74.75% similarity) WP_099010013.1(62.59% identity, 74.05% similarity) US_8759619_B2-27 (61.39% identity,72.41% similarity) APG05370.0 (61.01% identity, 72.28% similarity)US_8318900_B2-82 (59.07% identity, 71.35% similarity) APG00974.0 (59.03%identity, 69.83% similarity) APG06739.0 (58.77% identity, 70.5%similarity) APG07969.0 60, 65, 70, 75, 80, 85, APG08547.0 (39.17%identity, 56.97% 90, 91, 92, 93, 94, similarity) 95, 96, 97, 98, 99,APG02990.0 (39.13% identity, 57.1% 100 similarity) APG05787.0 (38.95%identity, 53.78% similarity) APG00371.0 US_2016_0355842_A1-72 (34.59%identity, 52.91% similarity) APG01148.0 (34.08% identity, 49.58%similarity) APG03638.0 (33.05% identity, 49.72% similarity) OOR71217.1(33.05% identity, 49.72% similarity) APG08605.0 (32.77% identity, 49.72%similarity) APG04182.0 (32.71% identity, 50.27% similarity) APG06273.0(32.47% identity, 49.43% similarity) APG08004.0 98, 99, 100 APG05715.0(95.21% identity, 97.48% similarity) APG02445.0 (94.46% identity, 96.73%similarity) WP_098807694.1 (94.46% identity, 96.73% similarity)APG04176.0 (93.97% identity, 95.98% similarity) APG04568.0 (91.73%identity, 94.74% similarity) APG06331.0 (89.05% identity, 92.29%similarity) WP_070144216.1 (88.44% identity, 93.72% similarity)APG07911.0 (87.56% identity, 91.04% similarity) APG00212.0US_2016_0311864_A1-75 (86.86% identity, 91.24% similarity) APG00806.0US_2017_0175134_A1-167 (86.43% identity, 92.71% similarity) APG08021.085, 90, 91, 92, 93, 94, WO_2017_146899-16 (68.29% identity, 95, 96, 97,98, 99, 78.52% similarity) 100 WO_2017_146899-21 (68.29% identity,78.52% similarity) AGV55017.1 (68.24% identity, 79.86% similarity)WO_2017_146899-26 (68.11% identity, 80.0% similarity) APG07037.0 (66.36%identity, 75.75% similarity) US_9567381_B2-232 (64.33% identity, 76.09%similarity) WP_016098327.1 (63.93% identity, 75.5% similarity)OUB62847.1 (63.19% identity, 76.11% similarity) US_9567381_B2-233(62.27% identity, 75.33% similarity) US_8461415_B2-30 (62.05% identity,74.74% similarity) APG08081.0 70, 75, 80, 85, 90, 91, WP_000836979.1(67.27% identity, 80.03% 92, 93, 94, 95, 96, similarity) 97, 98, 99, 100WP_087876328.1 (67.18% identity, 79.69% similarity) WP_016084067.1(66.9% identity, 80.19% similarity) WP_098633270.1 (52.96% identity,69.22% similarity) APG00078.0 US_2016_0366881_A1-1 (52.87% identity,69.29% similarity) WP_078178666.1 (52.75% identity, 68.99% similarity)WP_063549551.1 (51.75% identity, 68.41% similarity) WP_098342656.1(51.01% identity, 67.65% similarity) OUB31138.1 (50.68% identity, 65.71%similarity) APG00732.0 US_2016_0311864_A1-212 (50.14% identity, 56.78%similarity) APG08171.0 70, 75, 80, 85, 90, 91, APG09835.0 (92.2%identity, 94.43% 92, 93, 94, 95, 96, similarity) 97, 98, 99, 100APG06424.0 (92.19% identity, 96.21% similarity) APG08468.0 (91.31%identity, 94.88% similarity) APG08852.0 (90.42% identity, 93.99%similarity) OPA11477.1 (90.42% identity, 93.99% similarity) APG00184.0US_2016_0355842_A1-19 (63.17% identity, 65.62% similarity)WP_044306772.1 (63.17% identity, 65.62% similarity) WP_098816555.1(62.95% identity, 65.4% similarity) WP_097891175.1 (62.72% identity,65.4% similarity) WP_098217793.1 (62.72% identity, 65.4% similarity)APG08193.0 80, 85, 90, 91, 92, 93, WP_002114997.1 (71.25% identity,79.9% 94, 95, 96, 97, 98, similarity) 99, 100 APG04966.0 (70.23%identity, 81.17% similarity) WP_103458386.1 (69.9% identity, 77.81%similarity) WP_070128649.1 (69.72% identity, 78.37% similarity)WP_002191947.1 (68.22% identity, 77.0% similarity) WP_002166959.1(67.96% identity, 77.0% similarity) APG00988.0 US_2017_0175134_A1-194(67.61% identity, 78.41% similarity) APG07247.0 (67.18% identity, 79.9%similarity) WP_103458366.1 (67.18% identity, 79.9% similarity)APG00331.0 US_2016_0366881_A1-15 (66.16% identity, 75.57% similarity)APG08207.0 65, 70, 75, 80, 85, 90, APG04182.0 (56.18% identity, 63.99%91, 92, 93, 94, 95, similarity) 96, 97, 98, 99, 100 APG02990.0 (27.96%identity, 41.94% similarity) APG07969.0 (27.71% identity, 40.91%similarity) APG09091.0 (27.55% identity, 39.69% similarity) APG05787.0(27.53% identity, 41.08% similarity) APG03553.0 (26.78% identity, 37.83%similarity) APG01197.0 (26.57% identity, 38.19% similarity)US_2017_0137842_A1-2 (26.19% identity, 40.21% similarity) APG08547.0(26.12% identity, 40.69% similarity) US_8829279_B2-11 (26.02% identity,39.75% similarity) APG08240.0 90, 91, 92, 93, 94, 95, APG03760.0 (80.23%identity, 86.16% 96, 97, 98, 99, 100 similarity) WP_098584415.1 (80.23%identity, 86.16% similarity) APG00224.0 US_2016_0355842_A1-28 (76.14%identity, 84.09% similarity) APG07487.0 (72.73% identity, 80.4%similarity) US_8318900_B2-63 (67.61% identity, 80.0% similarity)APG05634.0 (67.52% identity, 77.78% similarity) WP_098775686.1 (67.52%identity, 77.78% similarity) APG05506.0 (67.04% identity, 79.05%similarity) APG00609.0 US_2017_0175134_A1-57 (64.57% identity, 76.57%similarity) US_8829279_B2-37 (62.98% identity, 74.86% similarity)APG08316.0 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75,80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100 APG08335.0 50, 55,60, 65, 70, 75, WP_084812867.1 (73.54% identity, 80.16% 80, 85, 90, 91,92, similarity) 93, 94, 95, 96, 97, WP_098398583.1 (30.99% identity,47.89% 98, 99, 100 similarity) CA_2844913-63 (29.41% identity, 47.9%similarity) CA_2844913-64 (29.41% identity, 47.9% similarity) APG08339.097, 98, 99, 100 US_7919272_B2-22 (93.18% identity, 96.75% similarity)WP_000730235.1 (92.53% identity, 96.75% similarity) WP_078203893.1(87.66% identity, 93.51% similarity) CA_2844913-62 (65.85% identity,75.38% similarity) APG08370.0 5, 10, 15, 20, 25, 30, WP_090462867.1(58.52% identity, 75.57% 35, 40, 45, 50, 55, similarity) 60, 65, 70, 75,80, 85, WP_076377347.1 (55.85% identity, 71.89% 90, 91, 92, 93, 94,similarity) 95, 96, 97, 98, 99, WP_090462870.1 (48.56% identity, 60.83%100 similarity) APG05471.0 (42.52% identity, 57.3% similarity)WP_008510716.1 (35.37% identity, 53.68% similarity) WP_090503345.1(30.2% identity, 43.0% similarity) WP_062550478.1 (28.47% identity,43.22% similarity) WP_074492263.1 (27.29% identity, 42.71% similarity)WP_078670461.1 (26.7% identity, 41.5% similarity) SKA29206.1 (26.37%identity, 41.27% similarity) APG08378.0 85, 90, 91, 92, 93, 94,APG07322.0 (100.0% identity, 100.0% 95, 96, 97, 98, 99, similarity) 100CA_2516349-51 (76.93% identity, 84.67% similarity) US_9567381_B2-373(69.57% identity, 78.73% similarity) US_8044266_132-3 (67.7% identity,77.43% similarity) CA_2516349-14 (67.56% identity, 77.43% similarity)CA_2516349-16 (67.28% identity, 77.01% similarity) US_9567381_B2-374(66.03% identity, 75.78% similarity) APG00204.0 US_2016_0177333_A1-89(65.14% identity, 75.04% similarity) WP_098223478.1 (64.84% identity,74.74% similarity) APG00695.0 US_2017_0175134_A1-109 (63.2% identity,74.48% similarity) APG08427.0 70, 75, 80, 85, 90, 91, WP_051903189.1(54.19% identity, 74.42% 92, 93, 94, 95, 96, similarity) 97, 98, 99, 100PIB64337.1 (53.56% identity, 71.95% similarity) WP_071482605.1 (51.81%identity, 71.27% similarity) WP_070996161.1 (51.71% identity, 69.48%similarity) WP_081041771.1 (51.71% identity, 69.48% similarity)WP_065950078.1 (48.55% identity, 66.22% similarity) WP_078835606.1(48.49% identity, 69.37% similarity) WP_101219829.1 (48.32% identity,66.0% similarity) APG00780.0 US_2016_0355842_A1-169 (48.1% identity,66.0% similarity) WP_060754610.1 (45.25% identity, 60.26% similarity)APG08468.0 65, 70, 75, 80, 85, 90, APG08852.0 (96.88% identity, 98.44%91, 92, 93, 94, 95, similarity) 96, 97, 98, 99, 100 APG09835.0 (96.88%identity, 98.44% similarity) OPA11477.1 (96.88% identity, 98.44%similarity) APG06424.0 (91.31% identity, 95.1% similarity) APG08171.0(91.31% identity, 94.88% similarity) APG00184.0 US_2016_0355842_A1-19(59.02% identity, 63.47% similarity) WP_044306772.1 (59.02% identity,63.47% similarity) WP_098816555.1 (58.8% identity, 63.25% similarity)WP_097891175.1 (58.57% identity, 63.25% similarity) WP_098217793.1(58.57% identity, 63.25% similarity) APG08495.0 60, 65, 70, 75, 80, 85,APG07176.0 (39.06% identity, 60.94% 90, 91, 92, 93, 94, similarity) 95,96, 97, 98, 99, APG09675.0 (37.34% identity, 53.48% 100 similarity)APG07983.0 (35.23% identity, 55.7% similarity) APG08526.0 90, 91, 92,93, 94, 95, APG00603.0 US_2016_0311864_A1-198 96, 97, 98, 99, 100(77.16% identity, 87.45% similarity) WP_097981477.1 (77.16% identity,87.45% similarity) WP_098887934.1 (76.95% identity, 87.24% similarity)APG01084.0 (75.93% identity, 88.27% similarity) WP_098867980.1 (74.49%identity, 84.57% similarity) APG05878.0 (67.28% identity, 83.13%similarity) WP_098505564.1 (61.93% identity, 71.19% similarity)APG01112.0 US_2017_0175134_A1-204 (61.32% identity, 70.16% similarity)WP_097891119.1 (33.33% identity, 36.42% similarity) APG00404.0US_2016_0311864_A1-169 (30.91% identity, 48.73% similarity) APG08576.094, 95, 96, 97, 98, 99, APG07780.0 (87.41% identity, 93.17% 100similarity) APG05984.0 (86.64% identity, 93.14% similarity) APG05943.0(75.59% identity, 83.39% similarity) APG09439.0 (74.82% identity, 85.61%similarity) APG03587.0 (73.84% identity, 84.95% similarity) APG01093.0(72.56% identity, 83.39% similarity) APG01273.0 (70.67% identity, 81.27%similarity) APG08917.0 (62.82% identity, 72.92% similarity) APG05992.0(57.76% identity, 71.12% similarity) APG01706.0 (56.83% identity, 68.71%similarity) APG08577.0 65, 70, 75, 80, 85, 90, APG04978.0 (43.32%identity, 60.65% 91, 92, 93, 94, 95, similarity) 96, 97, 98, 99, 100APG02115.0 (42.81% identity, 62.23% similarity) APG07463.0 (36.49%identity, 54.04% similarity) WP_073006288.1 (36.36% identity, 55.64%similarity) SHH89794.1 (35.25% identity, 54.68% similarity)WP_084723762.1 (32.44% identity, 50.84% similarity) WP_073006284.1(32.38% identity, 53.74% similarity) WP_073008904.1 (30.24% identity,49.14% similarity) APG08624.0 70, 75, 80, 85, 90, 91, WP_064675442.1(58.69% identity, 71.78% 92, 93, 94, 95, 96, similarity) 97, 98, 99, 100WP_095192304.1 (57.73% identity, 71.82% similarity) KKX69741.1 (56.95%identity, 69.7% similarity) WP_082240515.1 (56.95% identity, 69.48%similarity) PAM83583.1 (56.92% identity, 69.42% similarity)WP_046815282.1 (56.72% identity, 69.25% similarity) WP_093227613.1(56.49% identity, 72.21% similarity) WP_077044189.1 (55.36% identity,68.97% similarity) WP_025110931.1 (52.38% identity, 68.4% similarity)WP_102650854.1 (52.14% identity, 67.31% similarity) APG08625.0 92, 93,94, 95, 96, 97, APG04479.0 (89.74% identity, 91.5% 98, 99, 100similarity) WP_061885189.1 (33.89% identity, 48.46% similarity)WP_088118891.1 (33.89% identity, 48.46% similarity) APG08369.0 (33.04%identity, 51.19% similarity) WP_077997354.1 (32.11% identity, 49.01%similarity) APG00146.0 US_2016_0304898_A1-166 (31.3% identity, 49.86%similarity) WP_098582510.1 (31.3% identity, 49.86% similarity)APG00552.0 US_2016_0366881_A1-110 (31.3% identity, 47.37% similarity)APG03377.0 (30.97% identity, 49.15% similarity) APG02315.0 (30.37%identity, 45.85% similarity) APG08639.0 80, 85, 90, 91, 92, 93,APG02277.0 (59.78% identity, 76.09% 94, 95, 96, 97, 98, similarity) 99,100 WP_098733797.1 (51.09% identity, 70.65% similarity) WP_098069632.1(50.72% identity, 70.29% similarity) WP_098643607.1 (50.72% identity,69.93% similarity) US_8461421_B2-74 (50.53% identity, 69.4% similarity)APG00091.0 US_2016_0304898_A1-120 (50.18% identity, 69.4% similarity)APG03249.0 (49.82% identity, 68.68% similarity) APG03891.0 (49.64%identity, 68.84% similarity) WP_098652092.1 (49.64% identity, 68.84%similarity) WP_098099019.1 (48.91% identity, 68.84% similarity)APG00850.0 US_2016_0355842_A1-178 (48.91% identity, 68.12% similarity)WP_098208340.1 (48.91% identity, 68.12% similarity) APG08642.0 90, 91,92, 93, 94, 95, APG00543.0 US_2017_0175134_A1-31 96, 97, 98, 99, 100(80.49% identity, 85.85% similarity) AGV55018.1 (80.17% identity, 85.9%similarity) WP_016098322.1 (77.06% identity, 83.25% similarity)US_9567381_B2-246 (75.51% identity, 83.95% similarity) APG07810.0(40.15% identity, 53.45% similarity) WO_2014_102697-2 (37.46% identity,50.06% similarity) WO_2014_102697-60 (37.46% identity, 50.06%similarity) WO_2014_102697-1 (37.11% identity, 49.64% similarity)WO_2014_102697-3 (36.95% identity, 49.59% similarity) WO_2014_102697-61(36.95% identity, 49.59% similarity) APG08757.0 91, 92, 93, 94, 95, 96,APG04021.0 (81.43% identity, 90.86% 97, 98, 99, 100 similarity)APG09100.0 (81.14% identity, 89.71% similarity) US_8318900_B2-77 (47.09%identity, 62.05% similarity) APG04586.0 (46.81% identity, 61.77%similarity) APG06820.0 (42.36% identity, 59.25% similarity) APG06480.0(34.13% identity, 49.74% similarity) APG00718.0 US_2017_0175134_A1-132(33.89% identity, 48.33% similarity) APG00860.0 US_2016_0355842_A1-180(33.8% identity, 52.63% similarity) APG00586.0 US_2016_0355842_A1-135(32.97% identity, 53.68% similarity) APG00420.0 US_2016_0355842_A1-85(32.61% identity, 53.53% similarity) APG08765.0 55, 60, 65, 70, 75, 80,AIW52619.1 (37.1% identity, 51.08% 85, 90, 91, 92, 93, similarity) 94,95, 96, 97, 98, US_9567381_B2-424 (36.96% identity, 99, 100 51.08%similarity) US_8461421_B2-120 (36.79% identity, 51.87% similarity)APG00864.0 US_2017_0175134_A1-174 (36.76% identity, 51.34% similarity)APG00664.0 US_2017_0175134_A1-89 (35.62% identity, 49.22% similarity)US_8318900_B2-83 (34.93% identity, 49.73% similarity) APG03124.0 (34.78%identity, 48.58% similarity) APG04010.0 (34.69% identity, 48.28%similarity) ABW89734.1 (34.64% identity, 50.07% similarity)CA_2516349-43 (34.51% identity, 50.34% similarity) US_9567381_B2-34(34.51% identity, 50.34% similarity) WP_043939395.1 (34.51% identity,50.2% similarity) APG07972.0 (34.51% identity, 50.0% similarity)APG08791.0 96, 97, 98, 99, 100 APG06381.0 (93.19% identity, 95.05%similarity) WP_098783206.1 (89.47% identity, 91.33% similarity)APG08812.0 55, 60, 65, 70, 75, 80, PCJ04527.1 (33.9% identity, 55.93%85, 90, 91, 92, 93, similarity) 94, 95, 96, 97, 98, WP_015268242.1(31.88% identity, 53.02% 99, 100 similarity) PQJ72467.1 (31.72%identity, 54.14% similarity) WP_056615748.1 (31.16% identity, 51.03%similarity) WP_096527538.1 (31.06% identity, 53.58% similarity)WP_038918640.1 (30.95% identity, 53.06% similarity) APG05045.0 (30.72%identity, 49.02% similarity) WP_011306610.1 (30.0% identity, 55.0%similarity) WP_048041904.1 (29.51% identity, 52.46% similarity)US_9879277_B2-24 (29.35% identity, 52.22% similarity) APG08823.0 75, 80,85, 90, 91, 92, WP_002193629.1 (64.21% identity, 74.58% 93, 94, 95, 96,97, similarity) 98, 99, 100 APG01051.0 (64.0% identity, 73.67%similarity) WP_103458389.1 (61.69% identity, 71.1% similarity)APG00871.0 (59.03% identity, 72.58% similarity) APG00755.0US_2016_0355842_A1-166 (58.58% identity, 70.87% similarity)WP_098711954.1 (58.58% identity, 70.87% similarity) APG02980.0 (56.33%identity, 66.87% similarity) APG00402.0 US_2016_0355842_A1-77 (54.33%identity, 71.67% similarity) APG00583.0 US_2016_0366881_A1-114 (42.15%identity, 59.69% similarity) APG06065.0 (31.29% identity, 49.68%similarity) APG08852.0 65, 70, 75, 80, 85, 90, OPA11477.1 (100.0%identity, 100.0% 91, 92, 93, 94, 95, similarity) 96, 97, 98, 99, 100APG08468.0 (96.88% identity, 98.44% similarity) APG09835.0 (96.44%identity, 98.22% similarity) APG06424.0 (90.42% identity, 94.43%similarity) APG08171.0 (90.42% identity, 93.99% similarity) APG00184.0US_2016_0355842_A1-19 (58.35% identity, 63.03% similarity)WP_044306772.1 (58.35% identity, 63.03% similarity) WP_098816555.1(58.13% identity, 62.81% similarity) WP_097891175.1 (57.91% identity,62.81% similarity) WP_098217793.1 (57.91% identity, 62.81% similarity)APG08912.0 94, 95, 96, 97, 98, 99, APG03861.0 (88.25% identity, 93.37%100 similarity) WP_103623145.1 (88.25% identity, 93.37% similarity)APG07783.0 (87.65% identity, 92.77% similarity) APG03506.0 (83.63%identity, 88.99% similarity) APG09901.0 (81.44% identity, 88.92%similarity) APG00545.0 US_2016_0355842_A1-122 (32.47% identity, 47.7%similarity) APG00276.0 US_2016_0355842_A1-47 (32.27% identity, 48.55%similarity) APG08369.0 (31.92% identity, 44.92% similarity) APG00362.0US_2016_0366881_A1-33 (30.56% identity, 45.28% similarity)WP_079562232.1 (29.89% identity, 45.53% similarity) APG00552.0US_2016_0366881_A1-110 (29.89% identity, 43.58% similarity) APG09027.092, 93, 94, 95, 96, 97, APG06331.0 (85.32% identity, 91.79% 98, 99, 100similarity) APG04176.0 (85.07% identity, 91.29% similarity) APG07911.0(84.58% identity, 90.8% similarity) APG00306.0 US_2016_0366881_A1-3(83.82% identity, 88.97% similarity) APG00600.0 US_2016_0311864_A1-197(83.74% identity, 88.92% similarity) WP_099012321.1 (83.74% identity,88.92% similarity) APG08004.0 (82.09% identity, 88.56% similarity)APG05715.0 (81.59% identity, 89.3% similarity) APG02445.0 (81.59%identity, 88.81% similarity) WP_098807694.1 (81.59% identity, 88.81%similarity) APG09028.0 55, 60, 65, 70, 75, 80, WP_095156798.1 (48.22%identity, 58.31% 85, 90, 91, 92, 93, similarity) 94, 95, 96, 97, 98,APG09441.0 (45.64% identity, 54.5% 99, 100 similarity) APG01679.0(31.25% identity, 43.48% similarity) WP_048536362.1 (31.25% identity,43.48% similarity) APG03986.0 (30.11% identity, 43.46% similarity)APG00702.0 US_2017_0175134_A1-115 (30.0% identity, 43.38% similarity)APG00787.0 US_2017_0175134_A1-156 (29.63% identity, 42.11% similarity)APG02533.0 (29.27% identity, 39.24% similarity) APG00372.0US_2016_0311864_A1-150 (29.17% identity, 39.41% similarity) APG08243.0(29.09% identity, 41.12% similarity) APG09048.0 92, 93, 94, 95, 96, 97,WP_068985586.1 (87.61% identity, 91.45% 98, 99, 100 similarity)APG09392.0 (87.32% identity, 91.15% similarity) APG02862.0 (87.02%identity, 91.15% similarity) APG07381.0 (86.14% identity, 90.86%similarity) APG01874.0 (84.14% identity, 87.82% similarity) APG00417.0US_2016_0355842_A1-83 (76.61% identity, 84.8% similarity) WP_090695735.1(76.02% identity, 84.21% similarity) WP_071745741.1 (75.73% identity,83.92% similarity) APG00667.0 US_2016_0355842_A1-155 (61.66% identity,74.85% similarity) WP_098018654.1 (61.66% identity, 74.85% similarity)APG09152.0 90, 91, 92, 93, 94, 95, APG09904.0 (78.93% identity, 89.64%96, 97, 98, 99, 100 similarity) APG00850.0 US_2016_0355842_A1-178(76.79% identity, 87.86% similarity) WP_098208340.1 (76.79% identity,87.86% similarity) WP_098683229.1 (76.43% identity, 87.5% similarity)WP_098785528.1 (76.07% identity, 87.5% similarity) APG00021.0US_2016_0304898_A1-35 (70.0% identity, 84.29% similarity) APG00601.0US_2016_0355842_A1-141 (69.64% identity, 83.93% similarity) APG03249.0(69.64% identity, 83.57% similarity) APG03891.0 (69.29% identity, 83.57%similarity) WP_098634134.1 (69.29% identity, 83.21% similarity)APG09158.0 60, 65, 70, 75, 80, 85, WP_103655156.1 (45.4% identity,60.34% 90, 91, 92, 93, 94, similarity) 95, 96, 97, 98, 99, APG05653.0(44.31% identity, 59.18% 100 similarity) WP_098873359.1 (41.57%identity, 58.43% similarity) WP_098257700.1 (41.57% identity, 58.14%similarity) WP_097908332.1 (40.71% identity, 56.64% similarity)APG05385.0 (38.97% identity, 57.88% similarity) US_8461415_B2-32 (37.13%identity, 53.51% similarity) WP_091737463.1 (36.9% identity, 52.39%similarity) WP_086397082.1 (36.67% identity, 56.67% similarity)APG06364.0 (36.28% identity, 57.62% similarity) APG09181.0 55, 60, 65,70, 75, 80, APG06496.0 (40.08% identity, 63.97% 85, 90, 91, 92, 93,similarity) 94, 95, 96, 97, 98, US_9567381_B2-1430 (31.27% identity, 99,100 51.35% similarity) WP_088071735.1 (31.27% identity, 51.35%similarity) APG02790.0 (30.27% identity, 46.94% similarity) AJW76686.1(29.56% identity, 48.54% similarity) AGT15589.1 (29.46% identity, 51.16%similarity) US_9567381_B2-1427 (29.46% identity, 51.16% similarity)US_9567381_B2-1429 (29.46% identity, 51.16% similarity) WO_2017_199078-6(29.46% identity, 51.16% similarity) WP_043938803.1 (29.46% identity,51.16% similarity) ABV22504.1 (29.46% identity, 50.78% similarity)AFA52539.1 (29.46% identity, 50.78% similarity) APG09201.0 5, 10, 15,20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93,94, 95, 96, 97, 98, 99, 100 APG09210.0 60, 65, 70, 75, 80, 85,APG00275.0 US_2016_0311864_A1-111 90, 91, 92, 93, 94, (53.24% identity,54.05% similarity) 95, 96, 97, 98, 99, WP_098376074.1 (52.97% identity,54.05% 100 similarity) APG05139.0 (50.87% identity, 58.19% similarity)US_8318900_B2-90 (49.3% identity, 55.92% similarity) WP_044307627.1(41.53% identity, 42.86% similarity) WP_098816511.1 (41.31% identity,42.58% similarity) WP_098285136.1 (40.7% identity, 41.53% similarity)WP_097891341.1 (39.44% identity, 40.46% similarity) WP_098639056.1(39.19% identity, 40.2% similarity) WP_044307626.1 (38.93% identity,40.2% similarity) APG09221.0 99, 100 APG00038.0 US_2016_0355842_A1-1(96.22% identity, 98.02% similarity) APG06235.0 (89.75% identity, 93.53%similarity) APG00181.0 US_2016_0355842_A1-16 (89.57% identity, 94.06%similarity) APG00675.0 US_2017_0175134_A1-97 (60.49% identity, 74.83%similarity) APG00131.0 US_2016_0311864_A1-30 (59.54% identity, 75.62%similarity) WP_098631820.1 (59.54% identity, 75.62% similarity)APG07054.0 (50.61% identity, 67.54% similarity) APG00207.0US_2016_0311864_A1-70 (45.22% identity, 62.8% similarity) WP_098685831.1(45.22% identity, 62.8% similarity) AAK97481.1 (34.25% identity, 50.22%similarity) APG09238.0 99, 100 APG01474.0 (97.94% identity, 98.66%similarity) APG02585.0 (97.75% identity, 98.57% similarity) APG00766.0(55.57% identity, 71.15% similarity) APG07032.0 (54.16% identity, 70.79%similarity) APG04069.0 (53.74% identity, 69.23% similarity)CA_2844913-129 (53.29% identity, 68.9% similarity) CA_2844913-130(53.29% identity, 68.9% similarity) APG04672.0 (53.12% identity, 67.4%similarity) APG02031.0 (53.01% identity, 67.37% similarity)WP_016123960.1 (52.91% identity, 67.17% similarity) APG09266.0 90, 91,92, 93, 94, 95, APG00413.0 US_2016_0311864_A1-174 96, 97, 98, 99, 100(83.68% identity, 88.68% similarity) APG05717.0 (82.37% identity, 87.89%similarity) APG06920.0 (82.15% identity, 86.88% similarity) APG09069.0(80.53% identity, 86.58% similarity) US_9321814_B2-2 (80.26% identity,88.42% similarity) US_9321814_B2-3 (80.0% identity, 88.16% similarity)WP_098876539.1 (79.74% identity, 87.89% similarity) WP_017154552.1(79.0% identity, 88.19% similarity) US_9321814_B2-4 (78.42% identity,86.58% similarity) KEZ80012.1 (78.42% identity, 85.26% similarity)APG09289.0 50, 55, 60, 65, 70, 75, APG02309.0 (65.9% identity, 75.48%80, 85, 90, 91, 92, similarity) 93, 94, 95, 96, 97, APG02309.0 (65.9%identity, 75.48% 98, 99, 100 similarity) CA_2595901-10 (32.11% identity,45.96% similarity) CA_2595901-10 (32.11% identity, 45.96% similarity)CA_2595901-10 (32.11% identity, 45.96% similarity) US_5281530-4 (31.46%identity, 44.71% similarity) US_5281530-4 (31.46% identity, 44.71%similarity) US_5281530-4 (31.46% identity, 44.71% similarity)CA_2595901-11 (31.37% identity, 44.78% similarity) CA_2595901-11 (31.37%identity, 44.78% similarity) CA_2595901-11 (31.37% identity, 44.78%similarity) US_5281530-3 (31.37% identity, 44.78% similarity)US_5281530-3 (31.37% identity, 44.78% similarity) US_5281530-3 (31.37%identity, 44.78% similarity) APG09323.0 97, 98, 99, 100 APG00132.0US_2016_0311864_A1-31 (92.01% identity, 94.67% similarity) APG00249.0US_2016_0311864_A1-96 (91.57% identity, 96.14% similarity) APG00709.0(89.86% identity, 94.93% similarity) WP_098431222.1 (89.64% identity,94.22% similarity) APG00459.0 US_2016_0311864_A1-181 (87.29% identity,91.61% similarity) WP_098625308.1 (87.29% identity, 91.61% similarity)APG00065.0 US_2016_0177333_A1-31 (79.0% identity, 84.49% similarity)WP_097786723.1 (77.33% identity, 82.82% similarity) APG00229.0US_2016_0311864_A1-84 (75.0% identity, 82.62% similarity) APG00407.0US_2016_0311864_A1-170 (74.46% identity, 83.13% similarity)WP_098589502.1 (74.46% identity, 83.13% similarity) APG09412.0 80, 85,90, 91, 92, 93, APG00466.0 US_2016_0366881_A1-80 94, 95, 96, 97, 98,(64.09% identity, 78.95% similarity) 99, 100 WP_086405659.1 (64.09%identity, 78.95% similarity) WP_036654376.1 (42.04% identity, 57.06%similarity) ETK27180.1 (40.84% identity, 55.26% similarity)WP_061885189.1 (40.76% identity, 57.48% similarity) APG03377.0 (40.54%identity, 59.46% similarity) WP_088118891.1 (40.47% identity, 57.48%similarity) WP_077997354.1 (40.36% identity, 60.53% similarity)AVF26040.1 (40.36% identity, 60.24% similarity) APG04746.0 (39.34%identity, 56.46% similarity) APG09415.0 90, 91, 92, 93, 94, 95,WP_098157467.1 (100.0% identity, 100.0% 96, 97, 98, 99, 100 similarity)WP_097969898.1 (99.68% identity, 100.0% similarity) WP_071744311.1(99.68% identity, 99.68% similarity) WP_098786706.1 (99.05% identity,99.68% similarity) WP_070143005.1 (99.05% identity, 99.37% similarity)WP_098425855.1 (98.42% identity, 99.37% similarity) WP_025147991.1(98.11% identity, 99.68% similarity) WP_098304287.1 (96.85% identity,98.74% similarity) WP_001079819.1 (96.23% identity, 98.11% similarity)WP_098431274.1 (95.91% identity, 98.11% similarity) APG04316.0 (92.74%identity, 96.21% similarity) APG09416.0 96, 97, 98, 99, 100 APG00749.0(92.36% identity, 95.35% similarity) APG02638.0 (89.97% identity, 94.98%similarity) APG03379.0 (86.96% identity, 93.65% similarity) APG08088.0(80.46% identity, 89.4% similarity) APG00155.0 US_2016_0311864_A1-40(80.27% identity, 86.62% similarity) APG01127.0 (80.0% identity, 86.33%similarity) APG07655.0 (79.93% identity, 86.96% similarity) APG03504.0(79.93% identity, 86.62% similarity) APG09682.0 (79.8% identity, 86.09%similarity) APG08241.0 (79.26% identity, 86.29% similarity) APG07639.0(79.26% identity, 84.95% similarity) APG09430.0 93, 94, 95, 96, 97, 98,APG00149.0 US_2016_0304898_A1-172 99, 100 (89.91% identity, 92.92%similarity) WP_098326418.1 (89.91% identity, 92.92% similarity)WP_098902198.1 (40.03% identity, 56.23% similarity) APG00066.0US_2016_0177333_A1-33 (39.11% identity, 54.94% similarity) APG00081.0US_2016_0304898_A1-105 (38.9% identity, 56.41% similarity) APG07810.0(33.62% identity, 49.29% similarity) WO_2014_102697-1 (32.11% identity,49.93% similarity) WO_2014_102697-2 (32.03% identity, 50.21% similarity)WO_2014_102697-60 (32.03% identity, 50.21% similarity) APG01434.0(31.64% identity, 46.35% similarity) APG09439.0 90, 91, 92, 93, 94, 95,APG03587.0 (92.83% identity, 96.42% 96, 97, 98, 99, 100 similarity)APG07780.0 (77.34% identity, 86.69% similarity) APG05984.0 (77.34%identity, 86.33% similarity) APG08576.0 (74.82% identity, 85.61%similarity) APG01093.0 (72.3% identity, 82.37% similarity) APG05943.0(68.58% identity, 79.73% similarity) APG01273.0 (67.61% identity, 77.82%similarity) APG08917.0 (58.27% identity, 70.86% similarity) APG05992.0(52.88% identity, 69.06% similarity) APG01706.0 (51.25% identity, 64.87%similarity) APG09465.0 95, 96, 97, 98, 99, 100 APG02955.0 (89.67%identity, 94.96% similarity) APG07877.0 (88.92% identity, 93.95%similarity) APG09977.0 (80.9% identity, 84.92% similarity) APG00243.0US_2016_0311864_A1-94 (78.64% identity, 83.92% similarity)WP_074651503.1 (77.0% identity, 82.5% similarity) WP_097786723.1 (76.0%identity, 85.0% similarity) APG04176.0 (75.38% identity, 83.67%similarity) APG02445.0 (74.62% identity, 83.17% similarity)WP_098807694.1 (74.62% identity, 83.17% similarity) APG08004.0 (74.62%identity, 82.41% similarity) APG09511.0 99, 100 APG07866.0 (98.05%identity, 98.7% similarity) APG07224.0 (53.47% identity, 62.1%similarity) APG05103.0 (53.23% identity, 63.54% similarity) APG00457.0US_2016_0355842_A1-98 (42.83% identity, 56.22% similarity)WP_044307385.1 (42.83% identity, 56.22% similarity) WP_098376005.1(42.83% identity, 56.22% similarity) APG09895.0 (40.12% identity, 56.08%similarity) CA_2844913-200 (36.42% identity, 49.47% similarity)APG00348.0 US_2016_0366881_A1-26 (36.3% identity, 49.5% similarity)APG00348.0 US_2016_0366881_A1-26 (36.3% identity, 49.5% similarity)APG09548.0 55, 60, 65, 70, 75, 80, APG00123.0 US_2016_0304898_A1-146 85,90, 91, 92, 93, (43.62% identity, 54.0% similarity) 94, 95, 96, 97, 98,WP_098213628.1 (43.62% identity, 54.0% 99, 100 similarity) APG00721.0US_2017_0175134_A1-133 (37.1% identity, 49.22% similarity) APG00136.0US_2016_0177333_A1-75 (36.33% identity, 48.81% similarity)WP_097984025.1 (36.33% identity, 48.81% similarity) APG08350.0 (26.14%identity, 40.46% similarity) APG06056.0 (25.21% identity, 41.21%similarity) APG09675.0 80, 85, 90, 91, 92, 93, APG02088.0 (63.79%identity, 79.73% 94, 95, 96, 97, 98, similarity) 99, 100 APG07824.0(63.64% identity, 79.46% similarity) APG07732.0 (43.23% identity, 60.32%similarity) APG08495.0 (37.34% identity, 53.48% similarity) APG00598.0US_2016_0355842_A1-140 (36.28% identity, 51.52% similarity) APG05918.0(34.82% identity, 54.63% similarity) APG06880.0 (31.5% identity, 46.82%similarity) APG02592.0 (31.33% identity, 50.32% similarity) APG00236.0US_2016_0355842_A1-32 (31.31% identity, 51.76% similarity) APG07176.0(31.25% identity, 50.94% similarity) APG09773.0 80, 85, 90, 91, 92, 93,APG01165.0 (95.13% identity, 96.43% 94, 95, 96, 97, 98, similarity) 99,100 APG00296.0 US_2016_0355842_A1-52 (66.77% identity, 77.38%similarity) WP_098926211.1 (66.77% identity, 77.38% similarity)APG07033.0 (65.16% identity, 75.43% similarity) APG03264.0 (58.54%identity, 71.99% similarity) APG06221.0 (52.32% identity, 69.44%similarity) APG00553.0 US_2016_0355842_A1-124 (50.72% identity, 68.32%similarity) WP_098926212.1 (50.72% identity, 68.32% similarity)APG04454.0 (50.39% identity, 67.09% similarity) APG09727.0 (50.16%identity, 67.68% similarity) APG09812.0 98, 99, 100 WP_103108858.1(96.99% identity, 98.15% similarity) APG03438.0 (96.05% identity, 97.67%similarity) APG09824.0 (95.81% identity, 97.44% similarity) APG09936.0(95.81% identity, 96.51% similarity) APG04458.0 (94.42% identity, 96.51%similarity) KNB72291.1 (94.42% identity, 96.51% similarity) APG05978.0(94.29% identity, 95.21% similarity) APG04141.0 (93.72% identity, 96.51%similarity) WP_084765780.1 (92.71% identity, 94.53% similarity)WP_093871939.1 (37.53% identity, 58.43% similarity) APG09814.0 85, 90,91, 92, 93, 94, APG00965.0 (76.53% identity, 84.11% 95, 96, 97, 98, 99,similarity) 100 WP_001087511.1 (73.51% identity, 82.68% similarity)WP_097842690.1 (73.22% identity, 82.82% similarity) CA_2844913-12(73.07% identity, 82.68% similarity) WP_098209375.1 (73.07% identity,82.68% similarity) APG07954.0 (71.78% identity, 80.32% similarity)WP_098377949.1 (71.78% identity, 80.32% similarity) WP_098381669.1(71.78% identity, 80.17% similarity) EEM92570.1 (70.6% identity, 78.6%similarity) APG00152.0 US_2016_0311864_A1-36 (65.56% identity, 78.15%similarity) APG09826.0 90, 91, 92, 93, 94, 95, APG00424.0US_2016_0366881_A1-52 96, 97, 98, 99, 100 (82.76% identity, 85.66%similarity) WP_060852738.1 (82.76% identity, 85.66% similarity)APG03573.0 (82.0% identity, 86.1% similarity) WP_098349415.1 (81.93%identity, 85.15% similarity) APG00329.0 US_2016_0355842_A1-62 (79.65%identity, 82.78% similarity) WP_017762581.1 (77.93% identity, 81.32%similarity) APG07069.0 (76.74% identity, 83.01% similarity)WP_097891157.1 (75.49% identity, 82.47% similarity) US_8461415_B2-45(75.31% identity, 82.29% similarity) WP_044306759.1 (75.13% identity,82.11% similarity) APG09835.0 65, 70, 75, 80, 85, 90, APG08468.0 (96.88%identity, 98.44% 91, 92, 93, 94, 95, similarity) 96, 97, 98, 99, 100APG08852.0 (96.44% identity, 98.22% similarity) OPA11477.1 (96.44%identity, 98.22% similarity) APG08171.0 (92.2% identity, 94.43%similarity) APG06424.0 (91.76% identity, 94.88% similarity) APG00184.0US_2016_0355842_A1-19 (59.24% identity, 63.03% similarity)WP_044306772.1 (59.24% identity, 63.03% similarity) WP_098816555.1(59.02% identity, 62.81% similarity) WP_097891175.1 (58.8% identity,62.81% similarity) WP_098217793.1 (58.8% identity, 62.81% similarity)APG09895.0 75, 80, 85, 90, 91, 92, APG03986.0 (60.19% identity, 71.18%93, 94, 95, 96, 97, similarity) 98, 99, 100 APG00471.0US_2016_0366881_A1-85 (47.7% identity, 58.22% similarity) APG05538.0(44.92% identity, 58.31% similarity) APG00702.0 US_2017_0175134_A1-115(40.28% identity, 58.32% similarity) APG09511.0 (40.12% identity, 56.08%similarity) APG07866.0 (40.06% identity, 55.5% similarity) APG06995.0(39.32% identity, 52.23% similarity) APG05091.0 (38.96% identity, 52.15%similarity) APG00401.0 US_2016_0311864_A1-168 (38.44% identity, 53.29%similarity) APG00457.0 US_2016_0355842_A1-98 (37.42% identity, 54.04%similarity) WP_044307385.1 (37.42% identity, 54.04% similarity)WP_098376005.1 (37.42% identity, 54.04% similarity) APG09901.0 93, 94,95, 96, 97, 98, APG03506.0 (88.39% identity, 92.26% 99, 100 similarity)WP_103623145.1 (83.83% identity, 89.22% similarity) APG03861.0 (83.23%identity, 89.22% similarity) APG07783.0 (83.23% identity, 88.92%similarity) APG08912.0 (81.44% identity, 88.92% similarity) APG00276.0US_2016_0355842_A1-47 (32.86% identity, 46.29% similarity) APG08369.0(30.34% identity, 45.79% similarity) APG00545.0 US_2016_0355842_A1-122(30.0% identity, 44.0% similarity) WP_061885189.1 (29.97% identity,45.66% similarity) WP_088118891.1 (29.69% identity, 45.66% similarity)APG09903.0 45, 50, 55, 60, 65, 70, APG02986.0 (29.07% identity, 44.84%75, 80, 85, 90, 91, similarity) 92, 93, 94, 95, 96, US_8461421_B2-198(26.74% identity, 97, 98, 99, 100 40.52% similarity) US_8461421_B2-204(26.48% identity, 41.23% similarity) US_8461421_B2-180 (26.01% identity,40.32% similarity) US_8461421_B2-200 (25.27% identity, 38.62%similarity) APG10001.0 93, 94, 95, 96, 97, 98, OJG59749.1 (93.79%identity, 94.08% 99, 100 similarity) WP_086334647.1 (92.01% identity,92.01% similarity) WP_071867270.1 (91.72% identity, 92.01% similarity)WP_074798963.1 (91.42% identity, 91.72% similarity) APG07672.0 (90.83%identity, 92.6% similarity) WP_077151343.1 (89.94% identity, 91.42%similarity) WP_062805258.1 (89.64% identity, 91.12% similarity)WP_010735505.1 (89.35% identity, 91.12% similarity) WP_023519628.1(89.35% identity, 91.12% similarity) WP_019722830.1 (79.59% identity,87.57% similarity) APG01902.0 99, 100 WP_103108858.1 (99.31% identity,99.54% similarity) APG09824.0 (97.67% identity, 98.84% similarity)APG09812.0 (97.21% identity, 98.6% similarity) APG02996.0 (97.21%identity, 98.14% similarity) APG06717.0 (96.98% identity, 98.37%similarity) APG03098.0 (96.74% identity, 98.37% similarity) APG03438.0(96.74% identity, 98.37% similarity) APG04619.0 (96.05% identity, 97.91%similarity) APG09936.0 (96.05% identity, 97.21% similarity) APG05978.0(95.21% identity, 96.12% similarity) APG04458.0 (94.65% identity, 97.21%similarity) KNB72291.1 (94.65% identity, 97.21% similarity) APG04141.0(93.49% identity, 96.51% similarity) WP_084765780.1 (92.94% identity,95.22% similarity) APG04517.0 (91.2% identity, 94.44% similarity)APG02996.0 98, 99, 100 APG09812.0 (97.91% identity, 98.84% similarity)APG04619.0 (97.44% identity, 98.37% similarity) APG01902.0 (97.21%identity, 98.14% similarity) WP_103108858.1 (96.99% identity, 97.69%similarity) APG03098.0 (96.74% identity, 97.91% similarity) APG03438.0(96.74% identity, 97.91% similarity) APG06717.0 (96.28% identity, 97.67%similarity) APG09824.0 (96.28% identity, 97.44% similarity) APG09936.0(95.58% identity, 96.28% similarity) APG05978.0 (94.75% identity, 95.21%similarity) APG04458.0 (94.42% identity, 96.51% similarity) KNB72291.1(94.42% identity, 96.51% similarity) APG04141.0 (93.49% identity, 96.28%similarity) WP_084765780.1 (92.71% identity, 94.53% similarity)APG04517.0 (91.67% identity, 94.44% similarity) APG03098.0 100APG04619.0 (99.3% identity, 99.53% similarity) APG09824.0 (97.67%identity, 99.07% similarity) APG06717.0 (97.67% identity, 98.84%similarity) APG03438.0 (97.44% identity, 98.84% similarity) APG01902.0(96.74% identity, 98.37% similarity) APG02996.0 (96.74% identity, 97.91%similarity) WP_103108858.1 (96.53% identity, 97.92% similarity)APG05978.0 (96.35% identity, 97.03% similarity) APG09812.0 (96.28%identity, 97.91% similarity) APG09936.0 (95.12% identity, 96.74%similarity) KNB72291.1 (94.88% identity, 97.21% similarity) APG04458.0(94.65% identity, 97.21% similarity) APG04141.0 (93.49% identity, 96.98%similarity) WP_084765780.1 (93.17% identity, 95.22% similarity)APG04517.0 (91.2% identity, 94.91% similarity) APG04517.0 96, 97, 98,99, 100 APG04141.0 (92.34% identity, 95.59% similarity) KNB72291.1(91.88% identity, 94.9% similarity) APG09824.0 (91.67% identity, 95.37%similarity) APG03438.0 (91.67% identity, 94.91% similarity)WP_103108858.1 (91.67% identity, 94.68% similarity) APG02996.0 (91.67%identity, 94.44% similarity) APG04458.0 (91.65% identity, 94.66%similarity) APG06717.0 (91.44% identity, 95.14% similarity) APG09812.0(91.44% identity, 94.21% similarity) APG03098.0 (91.2% identity, 94.91%similarity) APG01902.0 (91.2% identity, 94.44% similarity) APG09936.0(91.18% identity, 93.27% similarity) WP_084765780.1 (90.87% identity,93.61% similarity) APG04619.0 (90.74% identity, 94.68% similarity)APG05978.0 (90.41% identity, 93.61% similarity) APG04619.0 99, 100APG03098.0 (99.3% identity, 99.53% similarity) APG02996.0 (97.44%identity, 98.37% similarity) APG03438.0 (97.21% identity, 98.84%similarity) APG09824.0 (96.98% identity, 98.6% similarity) APG06717.0(96.98% identity, 98.37% similarity) APG01902.0 (96.05% identity, 97.91%similarity) WP_103108858.1 (95.83% identity, 97.45% similarity)APG09812.0 (95.81% identity, 97.67% similarity) APG05978.0 (95.66%identity, 96.58% similarity) APG09936.0 (94.65% identity, 96.51%similarity) KNB72291.1 (94.42% identity, 96.98% similarity) APG04458.0(94.19% identity, 96.98% similarity) APG04141.0 (93.02% identity, 96.74%similarity) WP_084765780.1 (92.71% identity, 94.99% similarity)APG04517.0 (90.74% identity, 94.68% similarity) APG06717.0 100APG03098.0 (97.67% identity, 98.84% similarity) APG03438.0 (96.98%identity, 99.07% similarity) APG01902.0 (96.98% identity, 98.37%similarity) APG04619.0 (96.98% identity, 98.37% similarity)WP_103108858.1 (96.76% identity, 97.92% similarity) APG09824.0 (96.74%identity, 98.84% similarity) APG02996.0 (96.28% identity, 97.67%similarity) APG09812.0 (95.81% identity, 97.67% similarity) APG05978.0(95.43% identity, 96.8% similarity) KNB72291.1 (95.12% identity, 97.91%similarity) APG09936.0 (95.12% identity, 96.98% similarity) APG04458.0(94.88% identity, 97.67% similarity) APG04141.0 (93.49% identity, 97.21%similarity) WP_084765780.1 (93.39% identity, 95.9% similarity)APG04517.0 (91.44% identity, 95.14% similarity)

i. Classes of Pesticidal Proteins

The pesticidal proteins provided herein and the nucleotide sequencesencoding them are useful in methods for impacting pests. That is, thecompositions and methods of the invention find use in agriculture forcontrolling or killing pests, including pests of many crop plants. Thepesticidal proteins provided herein are toxin proteins from bacteria andexhibit activity against certain pests. The pesticidal proteins are fromseveral classes of toxins including Cry, Cyt, BIN, Mtx toxins. See, forexample, Table 1 for the specific protein classifications of the variousSEQ ID NOs provided herein. In addition, reference is made throughoutthis disclosure to Pfam database entries. The Pfam database is adatabase of protein families, each represented by multiple sequencealignments and a profile hidden Markov model. Finn et al. (2014) Nucl.Acid Res. Database Issue 42:D222-D230.

Bacillus thuringiensis (Bt) is a gram-positive bacterium that producesinsecticidal proteins as crystal inclusions during its sporulation phaseof growth. The proteinaceous inclusions of Bacillus thuringiensis (Bt)are called crystal proteins or δ-endotoxins (or Cry proteins), which aretoxic to members of the class Insecta and other invertebrates.Similarly, Cyt proteins are parasporal inclusion proteins from Bt thatexhibits hemolytic (Cytolitic) activity or has obvious sequencesimilarity to a known Cyt protein. These toxins are highly specific totheir target organism, are innocuous to humans, vertebrates, and plants.

The structure of the Cry toxins reveals five conserved amino acidblocks, concentrated mainly in the center of the domain or at thejunction between the domains. The Cry toxin consists of three domains,each with a specific function. Domain I is a seven α-helix bundle inwhich a central helix is completely surrounded by six outer helices.This domain is implicated in channel formation in the membrane. DomainII appears as a triangular column of three anti-parallel β-sheets, whichare similar to antigen-binding regions of immunoglobulins. Domain IIIcontains anti-parallel β-strands in a β sandwich form. The N-terminalpart of the toxin protein is responsible for its toxicity andspecificity and contains five conserved regions. The C-terminal part isusually highly conserved and probably responsible for crystal formation.See, for example, U.S. Pat. No. 8,878,007.

Strains of B. thuringiensis show a wide range of specificity againstdifferent insect orders (Lepidoptera, Diptera, Coleoptera, Hymenoptera,Homoptera, Phthiraptera or Mallophaga, and Acari) and otherinvertebrates (Nemathelminthes, Platyhelminthes, and Sarocomastebrates).The cry proteins have been classified into groups based on toxicity tovarious insect and invertebrate groups. Generally, Cry I demonstratestoxicity to lepidopterans, Cry II to lepidopterans and dipterans, CryIIIto coleopterans, Cry IV to dipterans, and Cry V and Cry VI to nematodes.New Cry proteins can be identified and assigned to a Cry group based onamino acid identity. See, for example, Bravo, A. (1997) J. of Bacteriol.179:2793-2801; Bravo et al. (2013) Microb. Biotechnol. 6:17-26, hereinincorporated by reference.

Over 750 different cry gene sequences have been classified into 73groups (Cry1-Cry73), with new members of this gene family continuing tobe discovered (Crickmore et al. (2014) www.btnomenclature.info/). Thecry gene family consists of several phylogentically non-related proteinfamilies that may have different modes of action: the family ofthree-domain Cry toxins, the family of mosquitocidal Cry toxins, thefamily of the binary-like toxins, and the Cyt family of toxins (Bravo etal., 2005). Some Bt strains produce additional insecticidal toxins, theVIP toxins. See, also, Cohen et al. (2011) J. Mol. Biol. 413:4-814;Crickmore et al. (2014) Bacillus thuringiensis toxin nomenclature, foundon the world wide web at lifesci.sussex.ac.uk/home/Neil_Crickmore/Bt/;Crickmore et al. (1988) Microbiol. Mol. Biol. Rev. 62: 807-813; Gill etal. (1992) Ann. Rev. Entomol. 37: 807-636; Goldbert et al. (1997) Appl.Environ. Microbiol. 63:2716-2712; Knowles et al. (1992) Proc. R. Soc.Ser. B. 248: 1-7; Koni et al. (1994) Microbiology 140: 1869-1880; Lailaket al. (2013) Biochem. Biophys. Res. Commun. 435: 216-221; Lopez-Diaz etal. (2013) Environ. Microbiol. 15: 3030-3039; Perez et al. (2007) Cell.Microbiol. 9: 2931-2937; Promdonkoy et al. (2003) Biochem. J. 374:255-259; Rigden (2009) FEBS Lett. 583: 1555-1560; Schnepf et al. (1998)Microbiol. Mol. Biol. Rev. 62: 775-806; Soberon et al. (2013) Peptides41: 87-93; Thiery et al. (1998) J. Am. Mosq. Control Assoc. 14: 472-476;Thomas et al. (1983) FEBS Lett. 154: 362-368; Wirth et al. (1997) Proc.Natl. Acad. Sci. U.S.A. 94: 10536-10540; Wirth et al (2005) Appl.Environ. Microbiol. 71: 185-189; and, Zhang et al. (2006) Biosci.Biotechnol. Biochem. 70: 2199-2204; each of which is herein incorporatedby reference in their entirety.

Cyt designates a parasporal crystal inclusion protein from Bacillusthuringiensis with cytolytic activity, or a protein with sequencesimilarity to a known Cyt protein. (Crickmore et al. (1998) Microbiol.Mol. Biol. Rev. 62: 807-813). The gene is denoted by cyt. These proteinsare different in structure and activity from Cry proteins (Gill et al.(1992) Annu. Rev. Entomol. 37: 615-636). The Cyt toxins were firstdiscovered in B. thuringiensis subspecies israelensis (Goldberg et al.(1977) Mosq. News. 37: 355-358). There are 3 Cyt toxin familiesincluding 11 holotype toxins in the current nomenclature (Crickmore etal. (2014) Bacillus thuringiensis toxin nomenclature found on the worldwide web at lifesci.sussex.ac.uk/home/Neil_Crickmore/Bt/). The majorityof the B. thuringiensis isolates with cyt genes show activity againstdipteran insects (particularly mosquitoes and black flies), but thereare also cyt genes that have been described in B. thuringiensis strainstargeting lepidopteran or coleopteran insects (Guerchicoff et al. (1997)Appl. Environ. Microbiol. 63: 2716-2721).

The structure of Cyt2A, solved by X-ray crystallography, shows a singledomain where two outer layers of α-helix wrap around a mixed β-sheet.Further available crystal structures of Cyt toxins support a conservedα-β structural model with two α-helix hairpins flanking a β-sheet corecontaining seven to eight β-strands. (Cohen et al. (2011) J. Mol. Biol.413: 80 4-814) Mutagenic studies identified β-sheet residues as criticalfor toxicity, while mutations in the helical domains did not affecttoxicity (Adang et al.; Diversity of Bacillus thuringiensis CrystalToxins and Mechanism of Action. In: T. S. Dhadialla and S. S. Gill, eds,Advances in Insect Physiology, Vol. 47, Oxford: Academic Press, 2014,pp. 39-87.) The representative domain of the Cyt toxin is a δ-endotoxin,Bac_thur_toxin (Pfam PF01338).

There are multiple proposed models for the mode of action of Cyt toxins,and it is still an area of active investigation. Some Cyt proteins(Cyt1A) have been shown to require the presence of accessory proteinsfor crystallization. Cyt1A and Cyt2A protoxins are processed bydigestive proteases at the same sites in the N- and C-termini to astable toxin core. Cyt toxins then interact with non-saturated membranelipids, such as phosphatidylcholine, phosphatidylethanolamine, andsphingomyelin. For Cyt toxins, pore-formation and detergent-likemembrane disruption have been proposed as non-exclusive mechanisms; andit is generally accepted that both may occur depending on toxinconcentration, with lower concentrations favoring oligomeric pores andhigher concentrations leading to membrane breaks. (Butko (2003) Appl.Environ. Microbiol. 69: 2415-2422) In the pore-formation model, the Cyttoxin binds to the cell membrane, inducing the formation ofcation-selective channels in the membrane vesicles leading tocolloid-osmotic lysis of the cell. (Knowles et al. (1989) FEBS Lett.244: 259-262; Knowles et al. (1992) Proc. R. Soc. Ser. B. 248: 1-7 andPromdonkoy et al. (2003) Biochem. J. 374: 255-259). In the detergentmodel, there is a nonspecific aggregation of the toxin on the surface ofthe lipid bilayer leading to membrane disassembly and cell death. (Butko(2003) supra; Manceva et al. (2005) Biochem. 44: 589-597).

Multiple studies have shown synergistic activity between Cyt toxins andother B. thuringiensis toxins, particularly the Cry, Bin, and Mtxtoxins. This synergism has even been shown to overcome an insect'sresistance to the other toxin. (Wirth 1997, Wirth 2005, Thiery 1998,Zhang 2006) The Cyt synergistic effect for Cry toxins is proposed toinvolve Cyt1A binding to domain II of Cry toxins in solution or on themembrane plane to promote formation of a Cry toxin pre-pore oligomer.Formation of this oligomer is independent of the Cyt oligomerization,binding or insertion. (Lailak 2013, Perez 2007, Lopez-Diaz 2013)

A number of pesticidal proteins unrelated to the Cry proteins areproduced by some strains of B. thuringiensis and B. cereus duringvegetative growth (Estruch et al. (1996) Proc Natl Acad Sci USA93:5389-5394; Warren et al. (1994) WO 94/21795). These vegetativeinsecticidal proteins, or Vips, do not form parasporal crystal proteinsand are apparently secreted from the cell. The Tips are presentlyexcluded from the Cry protein nomenclature because they are notcrystal-forming proteins. The term VIP is a misnomer in the sense thatsome B. thuringiensis Cry proteins are also produced during vegetativegrowth as well as during the stationary and sporulation phases, mostnotably Cry3Aa. The location of the Vip genes in the B. thuringiensisgenome has been reported to reside on large plasmids that also encodecry genes (Mesrati et al. (2005) FEMS Microbiol. Lett. 244(2):353-8). Aweb-site for the nomenclature of Bt toxins can be found on the worldwide web at lifesci.sussex.ac.uk with the path“/home/Neil_Crickmore/Bt/” and at: “btnomenclature.info/”. See also,Schnepf et al. (1998) Microbiol. Mol. Biol. Rev. 62(3):775-806. Suchreferences are herein incorporated by reference.

Vip genes can be classified into 4 categories. Some Vip genes formbinary two-component protein complexes; an “A” component is usually the“active” portion, and a “B” component is usually the “binding” portion.(Pfam pfam.xfam.org/family/PF03495). The Vip1 and Vip4 proteinsgenerally contain binary toxin B protein domains. Vip2 proteinsgenerally contain binary toxin A protein domains.

The Vip1 and Vip2 proteins are the two components of a binary toxin thatexhibits toxicity to coleopterans. Vip1Aa1 and Vip2Aa1 are very activeagainst corn rootworms, particularly Diabrotica virgifera and Diabroticalongicornis (Han et al. (1999) Nat. Struct. Biol. 6:932-936; Warren G W(1997) “Vegetative insecticidal proteins: novel proteins for control ofcorn pests” In: Carozzi N B, Koziel M (eds) Advances in insect control,the role of transgenic plants; Taylor & Francis Ltd, London, pp 109-21).The membrane-binding 95 kDa Vip1 multimer provides a pathway for the 52kDa vip2 ADP-ribosylase to enter the cytoplasm of target western cornrootworm cells (Warren (1997) supra). The NAD-dependentADP-ribosyltransferase Vip2 likely modifies monomeric actin at Arg177 toblock polymerization, leading to loss of the actin cytoskeleton andeventual cell death due to the rapid subunit ex-change within actinfilaments in vivo (Carlier M. F. (1990) Adv. Biophys. 26:51-73).

Like Cry toxins, activated Vip3A toxins are pore-forming proteinscapable of making stable ion channels in the membrane (Lee et al. (2003)Appl. Environ. Microbiol. 69:4648-4657). Vip3 proteins are activeagainst several major lepidopteran pests (Rang et al. (2005) Appl.Environ. Microbiol. 71(10):6276-6281; Bhalla et al. (2005) FEMSMicrobiol. Lett. 243:467-472; Estruch et al. (1998) WO 9844137; Estruchet al. (1996) Proc Natl Acad Sci USA 93:5389-5394; Selvapandiyan et al.(2001) Appl. Environ Microbiol. 67:5855-5858; Yu et al. (1997) Appl.Environ Microbiol. 63:532-536). Vip3A is active against Agrotis ipsilon,Spodoptera frugiperda, Spodoptera exigua, Heliothis virescens, andHelicoverpa zea (Warren et al. (1996) WO 96/10083; Estruch et al. (1996)Proc Natl Acad Sci USA 93:5389-5394). Like Cry toxins, Vip3A proteinsmust be activated by proteases prior to recognition at the surface ofthe midgut epithelium of specific membrane proteins different from thoserecognized by Cry toxins.

The MTX family of toxin proteins is characterized by the presence of aconserved domain, ETX_MTX2 (pfam 03318). Members of this family sharesequence homology with the mosquitocidal toxins Mtx2 and Mtx3 fromBacillus sphaericus, as well as with the epsilon toxin ETX fromClostridium perfringens (Cole et al. (2004) Nat. Struct. Mol. Biol. 11:797-8; Thanabalu et al. (1996) Gene 170:85-9). The MTX-like proteins arestructurally distinct from the three-domain Cry toxins, as they have anelongated and predominately β-sheet-based structure. However, similar tothe three-domain toxins, the MTX-like proteins are thought to form poresin the membranes of target cells (Adang et al. (2014) supra). Unlike thethree-domain Cry proteins, the MTX-like proteins are much smaller inlength, ranging from 267 amino acids (Cry23) to 340 amino acids(Cry15A).

The protein family of MTX-like toxins is a relatively small classcompared to the three-domain Cry family (Crickmore et al. (2014) supra;Adang et al. (2014) supra). The members of the MTX-like toxin familyinclude Cry15, Cry23, Cry33, Cry38, Cry45, Cry46, Cry51, Cry60A, Cry60B,and Cry64. This family exhibits a range of insecticidal activity,including activity against insect pests of the Lepidopteran andColeopteran orders. Some members of this family may form binarypartnerships with other proteins, which may or may not be required forinsecticidal activity.

Cry15 is a 34 kDA protein that was identified in Bacillus thuringiensisserovar thompsoni HD542; it occurs naturally in a crystal together withan unrelated protein of approximately 40 kDa. The gene encoding Cry15and its partner protein are arranged together in an operon. Cry15 alonehas been shown to have activity against lepidopteran insect pestsincluding Manduca sexta, Cydia pomonella, and Pieris rapae, with thepresence of the 40 kDA protein having been shown to increase activity ofCry15 only against C. pomonella (Brown K. and Whiteley H. (1992) J.Bacteriol. 174:549-557; Naimov et al. (2008) Appl. Environ. Microbiol.74:7145-7151). Further studies are needed to elucidate the function ofthe partner protein of Cry15. Similarly, Cry23 is a 29 kDA protein thathas been shown to have activity against the coleopteran pests Triboliumcastaneum and Popillia japonica together with its partner protein Cry37(Donovan et al. (2000) U.S. Pat. No. 6,063,756).

New members of the MTX-like family are continuing to be identified. AnETX_MTX toxin gene was recently identified in the genome of Bacillusthuringiensis serovar tolworthi strain Na205-3. This strain was found tobe toxic against the lepidpoteran pest Helicoverpa armigera, and it alsocontained homologs of Cry1, Cry11, Vip1, Vip2, and Vip3 (Palma et al.(2014) Genome Announc. 2(2): e00187-14. Published online Mar. 13, 2014at doi: 10.1128/genomeA.00187-14; PMCID: PMC3953196). Because theMTX-like proteins have a unique domain structure relative to thethree-domain Cry proteins, they are believed to possess a unique mode ofaction, thereby making them a valuable tool in insect control and thefight against insect resistance.

Bacterial cells produce large numbers of toxins with diverse specificityagainst host and non-host organisms. Large families of binary toxinshave been identified in numerous bacterial families, including toxinsthat have activity against insect pests. (Poopathi and Abidha (2010) J.Physiol. Path. 1(3): 22-38). Lysinibacillus sphaericus (Ls), formerlyBacillus sphaericus, (Ahmed et al. (2007) Int. J. Syst. Evol. Microbiol.57:1117-1125) is well-known as an insect biocontrol strain. Ls producesseveral insecticidal proteins, including the highly potent binarycomplex BinA/BinB. This binary complex forms a parasporal crystal in Lscells and has strong and specific activity against dipteran insects,specifically mosquitoes. In some areas, insect resistance to existing Lsmosquitocidal strains has been reported. The discovery of new binarytoxins with different target specificity or the ability to overcomeinsect resistance is of significant interest.

The Ls binary insecticidal protein complex contains two majorpolypeptides, a 42 kDa polypeptide and a 51 kDa polypeptide, designatedBinA and BinB, respectively (Ahmed et al. (2007) supra). The twopolypeptides act synergistically to confer toxicity to their targets.Mode of action involves binding of the proteins to receptors in thelarval midgut. In some cases, the proteins are modified by proteasedigestion in the larval gut to produce activated forms. The BinBcomponent is thought to be involved in binding, while the BinA componentconfers toxicity (Nielsen-LeRoux et al. (2001) Appl. Environ. Microbiol.67(11):5049-5054). When cloned and expressed separately, the BinAcomponent is toxic to mosquito larvae, while the BinB component is not.However, co-administration of the proteins markedly increases toxicity(Nielsen-LeRoux et al. (2001) supra).

A small number of Bin protein homologs have been described frombacterial sources. Priest et al. (1997) Appl. Environ. Microbiol.63(4):1195-1198 describe a hybridization effort to identify new Lsstrains, although most of the genes they identified encoded proteinsidentical to the known BinA/BinB proteins. The BinA protein contains adefined conserved domain known as the Toxin 10 superfamily domain. Thistoxin domain was originally defined by its presence in BinA and BinB.The two proteins both have the domain, although the sequence similaritybetween BinA and BinB is limited in this region (<40%). The Cry49Aaprotein, which also has insecticidal activity, also has this domain(described below).

The Cry48Aa/Cry49Aa binary toxin of Ls has the ability to kill Culexquinquefasciatus mosquito larvae. These proteins are in a proteinstructural class that has some similarity to the Cry protein complex ofBacillus thuringiensis (Bt), a well-known insecticidal protein family.The Cry34/Cry35 binary toxin of Bt is also known to kill insects,including Western corn rootworm, a significant pest of corn. Cry34, ofwhich several variants have been identified, is a small (14 kDa)polypeptide, while Cry35 (also encoded by several variants) is a 44 kDapolypeptide. These proteins have some sequence homology with theBinA/BinB protein group and are thought to be evolutionarily related(Ellis et al. (2002) Appl. Environ. Microbiol. 68(3):1137-1145).

Phosphoinositide phospholipase C proteins (PI-PLC; alsophosphotidylinositol phospholipase C) are members of the broader groupof phospholipase C proteins. Many of these proteins play important rolesin signal transduction as part of normal cell physiology. Severalimportant bacterial toxins also contain domains with similarity to theseproteins (Titball, R. W. (1993) Microbiological Reviews. 57(2):347-366).Importantly, these proteins are implicated in signal amplificationduring intoxication of insect cells by Bt Cry proteins (Valaitis, A. P.(2008) Insect Biochemistry and Molecular Biology. 38: 611-618).

The PI-PLC toxin class occurs in Bacillus isolates, commonly seen inco-occurrence with homologs to other described toxin classes, such asBinary Toxins. This class of sequences has homology tophosphatidylinositol phosphodiesterases (also referred to asphosphatidylinositol-specific phospholipase C—PI-PLC). The crystalstructure and its active site were solved for B. cereus PI-PLC by Heinzet al (Heinz, et. al., (1995) The EMBO Journal. 14(16): 3855-3863). Theroles of the B. cereus PI-PLC active site amino acid residues incatalysis and substrate binding were investigated by Gässler et al usingsite-directed mutagenesis, kinetics, and crystal structure analysis(Gässler, et. al., (1997) Biochemistry. 36(42):12802-13).

These PI-PLC toxin proteins contain a PLC-like phosphodiesterase, TIMbeta/alpha-barrel domain (IPR017946) and/or a Phospholipase C,phosphatidylinositol-specific, X domain (IPR000909) (also referred to asthe PI-PLC X-box domain). We have also seen proteins with these domainsin combination with other typical Bacillus protein toxin domains. Thislist includes most commonly a lectin domain (IPR000772), a sugar-bindingdomain that can be present in one or more copies and is thought to bindcell membranes, as well as the Insecticidal crystal toxin (IPR008872)(also referred to as Toxin10 or P42), which is the defining domain ofthe Binary Toxin.

Previously, toxins of this PI-PLC class were defined in U.S. Pat. No.8,318,900 B2 SEQ ID NOs: 30 (DNA) and 79 (amino acid), in U.S. PatentPublication No. 20110263488A1 SEQ ID NOs: 8 (DNA) and 9 (amino acid),and in U.S. Pat. No. 8,461,421B2 SEQ ID NOs: 3 (DNA) and 63 (aminoacid).

Provided herein are pesticidal proteins from these classes of toxins.The pesticidal proteins are classified by their structure, homology toknown toxins and/or their pesticidal specificity.

ii. Variants and Fragments of Pesticidal Proteins and PolynucleotidesEncoding the Same

Pesticidal proteins or polypeptides of the invention include those setforth in SEQ ID NOs: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15,16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33,34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51,52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69,70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87,88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104,105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118,119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132,133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146,147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160,161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174,175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188,189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202,203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216,217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230,231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244,245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258,259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272,273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286,287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300,301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314,315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328,329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342,343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356,357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370,371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384,385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397, 398,399, 400, 401, 402, 403, 404, 405, 406, 407, 408, 409, 410, 411, 412,413, 414, 415, 416, 417, 418, 419, 420, 421, 422, 423, 424, 425, 426,427, 428, 429, 430, 431, 432, 433, 434, 435, 436, 437, 438, 439, 440,441, 442, 443, 444, 445, 446, 447, 448, 449, 450, 451, 452, 453, 454,455, 456, 457, 458, 459, 460, 461, 462, 463, 464, 465, 466, 467, 468,469, 470, 471, 472, 473, 474, 475, 476, 477, 478, 479, 480, 481, 482,483, 484, 485, 486, 487, 488, 489, 490, 491, 492, 493, 494, 495, 496,497, 498, 499, 500, 501, 502, 503, 504, 505, 506, 507, 508, 509, 510,511, 512, 513, 514, 515, 516, 517, 518, 519, 520, 521, 522, 523, 524,and/or 525, and fragments and variants thereof. By “pesticidal toxin” or“pesticidal protein” or “pesticidal polypeptide” is intended a toxin orprotein or polypeptide that has activity against one or more pests,including, insects, fungi, nematodes, and the like such that the pest iskilled or controlled.

An “isolated” or “purified” polypeptide or protein, or biologicallyactive portion thereof, is substantially or essentially free fromcomponents that normally accompany or interact with the polypeptide orprotein as found in its naturally occurring environment. Thus, anisolated or purified polypeptide or protein is substantially free ofother cellular material, or culture medium when produced by recombinanttechniques, or substantially free of chemical precursors or otherchemicals when chemically synthesized. A protein that is substantiallyfree of cellular material includes preparations of protein having lessthan about 30%, 20%, 10%, 5%, or 1% (by dry weight) of contaminatingprotein. When the protein of the invention or biologically activeportion thereof is recombinantly produced, optimally culture mediumrepresents less than about 30%, 20%, 10%, 5%, or 1% (by dry weight) ofchemical precursors or non-protein-of-interest chemicals.

The term “fragment” refers to a portion of a polypeptide sequence of theinvention. “Fragments” or “biologically active portions” includepolypeptides comprising a sufficient number of contiguous amino acidresidues to retain the biological activity, i.e., have pesticidalactivity. Fragments of the pesticidal proteins include those that areshorter than the full-length sequences, either due to the use of analternate downstream start site, or due to processing that produces ashorter protein having pesticidal activity. Processing may occur in theorganism the protein is expressed in, or in the pest after ingestion ofthe protein. Examples of fragments of the proteins can be found inTable 1. A biologically active portion of a pesticidal protein can be apolypeptide that is, for example, 10, 25, 50, 100, 150, 200, 250 or moreamino acids in length of any one of SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8,9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26,27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44,45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62,63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80,81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98,99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112,113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126,127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140,141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154,155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168,169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182,183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196,197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210,211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224,225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238,239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252,253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266,267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280,281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294,295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308,309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322,323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336,337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350,351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364,365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378,379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392,393, 394, 395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406,407, 408, 409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420,421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434,435, 436, 437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448,449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 461, 462,463, 464, 465, 466, 467, 468, 469, 470, 471, 472, 473, 474, 475, 476,477, 478, 479, 480, 481, 482, 483, 484, 485, 486, 487, 488, 489, 490,491, 492, 493, 494, 495, 496, 497, 498, 499, 500, 501, 502, 503, 504,505, 506, 507, 508, 509, 510, 511, 512, 513, 514, 515, 516, 517, 518,519, 520, 521, 522, 523, 524, and/or 525. Such biologically activeportions can be prepared by recombinant techniques and evaluated forpesticidal activity. As used here, a fragment comprises at least 8contiguous amino acids of SEQ ID NOs: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11,12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29,30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47,48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65,66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83,84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100,101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114,115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128,129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142,143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156,157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170,171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184,185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198,199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212,213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226,227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240,241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254,255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268,269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282,283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296,297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310,311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324,325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338,339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352,353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366,367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380,381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394,395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406, 407, 408,409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421, 422,423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435, 436,437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449, 450,451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 461, 462, 463, 464,465, 466, 467, 468, 469, 470, 471, 472, 473, 474, 475, 476, 477, 478,479, 480, 481, 482, 483, 484, 485, 486, 487, 488, 489, 490, 491, 492,493, 494, 495, 496, 497, 498, 499, 500, 501, 502, 503, 504, 505, 506,507, 508, 509, 510, 511, 512, 513, 514, 515, 516, 517, 518, 519, 520,521, 522, 523, 524, and/or 525.

Bacterial genes, including those encoding the pesticidal proteinsdisclosed herein, quite often possess multiple methionine initiationcodons in proximity to the start of the open reading frame. Often,translation initiation at one or more of these start codons will lead togeneration of a functional protein. These start codons can include ATGcodons. However, bacteria such as Bacillus sp. also recognize the codonGTG as a start codon, and proteins that initiate translation at GTGcodons contain a methionine at the first amino acid. On rare occasions,translation in bacterial systems can initiate at a TTG codon, though inthis event the TTG encodes a methionine. Furthermore, it is not oftendetermined a priori which of these codons are used naturally in thebacterium. Thus, it is understood that use of one of the alternatemethionine codons may also lead to generation of pesticidal proteins.These pesticidal proteins are encompassed in the present invention andmay be used in the methods disclosed herein. It will be understood that,when expressed in plants, it will be necessary to alter the alternatestart codon to ATG for proper translation.

In various embodiments the pesticidal proteins provided herein includeamino acid sequences deduced from the full-length nucleotide sequencesand amino acid sequences that are shorter than the full-length sequencesdue to the use of an alternate downstream start site. Thus, thenucleotide sequence of the invention and/or vectors, host cells, andplants comprising the nucleotide sequence of the invention (and methodsof making and using the nucleotide sequence of the invention) maycomprise a nucleotide sequence encoding an alternate start site.

It is recognized that modifications may be made to the pesticidalpolypeptides provided herein creating variant proteins. Changes designedby man may be introduced through the application of site-directedmutagenesis techniques. Alternatively, native, as yet-unknown or as yetunidentified polynucleotides and/or polypeptides structurally and/orfunctionally-related to the sequences disclosed herein may also beidentified that fall within the scope of the present invention.Conservative amino acid substitutions may be made in nonconservedregions that do not alter the function of the pesticidal proteins.Alternatively, modifications may be made that improve the activity ofthe toxin. Modification of Cry toxins by domain III swapping hasresulted in some cases in hybrid toxins with improved toxicities againstcertain insect species. Thus, domain III swapping could be an effectivestrategy to improve toxicity of Cry toxins or to create novel hybridtoxins with toxicity against pests that show no susceptibility to theparental Cry toxins. Site-directed mutagenesis of domain II loopsequences may result in new toxins with increased insecticidal activity.Domain II loop regions are key binding regions of initial Cry toxinsthat are suitable targets for the mutagenesis and selection of Crytoxins with improved insecticidal properties. Domain I of the Cry toxinmay be modified to introduce protease cleavage sites to improve activityagainst certain pests. Strategies for shuffling the three differentdomains among large numbers of cry genes and high through outputbioassay screening methods may provide novel Cry toxins with improved ornovel toxicities.

As indicated, fragments and variants of the polypeptides disclosedherein will retain pesticidal activity. Pesticidal activity comprisesthe ability of the composition to achieve an observable effectdiminishing the occurrence or an activity of the target pest, includingfor example, bringing about death of at least one pest, or a noticeablereduction in pest growth, feeding, or normal physiological development.Such decreases in numbers, pest growth, feeding or normal developmentcan comprise any statistically significant decrease, including, forexample a decrease of about 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%,50%, 55%, 60%, 65%, 70%, 75%, 85%, 90%, 95% or greater. The pesticidalactivity against one or more of the various pests provided herein,including, for example, pesticidal activity against Coleoptera, Diptera,Hymenoptera, Lepidoptera, Mallophaga, Homoptera, Hemiptera, Orthroptera,Nematodes, Thysanoptera, Dermaptera, Isoptera, Anoplura, Siphonaptera,Trichoptera, etc., or any other pest described herein. It is recognizedthat the pesticidal activity may be different or improved relative tothe activity of the native protein, or it may be unchanged, so long aspesticidal activity is retained. Methods for measuring pesticidalactivity are provided elsewhere herein. See also, Czapla and Lang (1990)J. Econ. Entomol. 83:2480-2485; Andrews et al. (1988) Biochem. J.252:199-206; Marrone et al. (1985) J. of Economic Entomology 78:290-293;and U.S. Pat. No. 5,743,477, all of which are herein incorporated byreference in their entirety.

By “variants” is intended polypeptides having an amino acid sequencethat is at least about 60%, about 65%, about 70%, about 75%, about 80%,about 85%, about 86%, about 87%, about 88%, about 89%, about 90%, about91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%,about 98% or about 99% identical to the amino acid sequence of any ofSEQ ID NOs: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17,18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35,36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53,54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71,72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89,90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105,106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119,120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133,134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147,148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161,162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175,176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189,190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203,204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217,218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231,232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245,246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259,260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273,274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287,288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301,302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315,316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329,330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343,344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357,358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371,372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385,386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397, 398, 399,400, 401, 402, 403, 404, 405, 406, 407, 408, 409, 410, 411, 412, 413,414, 415, 416, 417, 418, 419, 420, 421, 422, 423, 424, 425, 426, 427,428, 429, 430, 431, 432, 433, 434, 435, 436, 437, 438, 439, 440, 441,442, 443, 444, 445, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455,456, 457, 458, 459, 460, 461, 462, 463, 464, 465, 466, 467, 468, 469,470, 471, 472, 473, 474, 475, 476, 477, 478, 479, 480, 481, 482, 483,484, 485, 486, 487, 488, 489, 490, 491, 492, 493, 494, 495, 496, 497,498, 499, 500, 501, 502, 503, 504, 505, 506, 507, 508, 509, 510, 511,512, 513, 514, 515, 516, 517, 518, 519, 520, 521, 522, 523, 524, and/or525, and retain pesticidal activity. Note, Table 1 provides non-limitingexamples of variant polypeptides (and polynucleotide encoding the same)for each of SEQ ID NOs: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14,15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32,33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50,51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68,69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86,87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103,104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117,118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131,132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145,146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159,160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173,174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187,188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201,202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215,216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229,230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243,244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257,258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271,272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285,286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299,300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313,314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327,328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341,342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355,356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369,370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383,384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397,398, 399, 400, 401, 402, 403, 404, 405, 406, 407, 408, 409, 410, 411,412, 413, 414, 415, 416, 417, 418, 419, 420, 421, 422, 423, 424, 425,426, 427, 428, 429, 430, 431, 432, 433, 434, 435, 436, 437, 438, 439,440, 441, 442, 443, 444, 445, 446, 447, 448, 449, 450, 451, 452, 453,454, 455, 456, 457, 458, 459, 460, 461, 462, 463, 464, 465, 466, 467,468, 469, 470, 471, 472, 473, 474, 475, 476, 477, 478, 479, 480, 481,482, 483, 484, 485, 486, 487, 488, 489, 490, 491, 492, 493, 494, 495,496, 497, 498, 499, 500, 501, 502, 503, 504, 505, 506, 507, 508, 509,510, 511, 512, 513, 514, 515, 516, 517, 518, 519, 520, 521, 522, 523,524, and/or 525. A biologically active variant of a pesticidalpolypeptide of the invention may differ by as few as about 1-15 aminoacid residues, as few as about 1-10, such as about 6-10, as few as 5, asfew as 4, as few as 3, as few as 2, or as few as 1 amino acid residue.In specific embodiments, the polypeptides can comprise an N-terminal ora C-terminal truncation, which can comprise at least a deletion of 10,15, 20, 25, 30, 35, 40, 45, 50 amino acids or more from either the N orC terminal of the polypeptide.

Table 2 provides protein domains found in SEQ ID NOs: 1-525 based onPFAM data. Both the domain description and the positions within a givenSEQ ID NO are provided in Table 2. In specific embodiments, the activevariant comprising any one of SEQ ID NOs: 1-525 can comprise at least70%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%,88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% sequenceidentity to any one of SEQ ID NOs: 1-525 and further comprises at leastone of the conserved domains set forth in Table 2. For example, in oneembodiment, the active variant will comprise at least 70%, 75%, 76%,77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%,91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% sequence identity to SEQID NO:1, and further comprises the native amino acids at positions267-329.

TABLE 2 Summary of PFAM domains in each of SEQ ID NOs: 1-525 SeqModification APG ID ID Type Accession Description Start Stop APG00751.01 PF03945 delta endotoxin, N- 134 244 terminal domain PF03945 deltaendotoxin, N- 267 329 terminal domain PF00555 delta endotoxin 337 517PF03944 delta endotoxin 549 690 APG00751.1 2 3′ Truncation PF03945 deltaendotoxin, N- 133 244 terminal domain PF03945 delta endotoxin, N- 267329 terminal domain PF00555 delta endotoxin 337 517 PF03944 deltaendotoxin 549 690 APG00751 3 no PFAM CryBP1 domains (APG04508.0)APG00831.0 4 PF03945 delta endotoxin, N- 90 303 terminal domain PF00555delta endotoxin 311 524 PF03944 delta endotoxin 534 674 APG00831.1 5Alternate PF03945 delta endotoxin, N- 84 297 start terminal domainPF00555 delta endotoxin 305 518 PF03944 delta endotoxin 528 668APG00831.2 6 Alternate PF03945 delta endotoxin, N- 84 297 start and 3′terminal domain Truncation PF00555 delta endotoxin 305 397 PF03944 deltaendotoxin 528 668 APG00832.0 7 PF03945 delta endotoxin, N- 103 291terminal domain PF00555 delta endotoxin 309 506 PF03944 delta endotoxin523 665 APG00832.1 8 3′ Truncation PF03945 delta endotoxin, N- 103 291terminal domain PF00555 delta endotoxin 309 506 PF03944 delta endotoxin523 663 APG00832 9 no PFAM CryBP1 domains (APG04850.0) APG00842.0 10PF03318 Clostridium epsilon 113 256 toxin ETX/Bacillus mosquitocidaltoxin MTX2 APG00842.1 11 Alternate PF03318 Clostridium epsilon 96 239start toxin ETX/Bacillus mosquitocidal toxin MTX2 APG00887.0 12 PF03318Clostridium epsilon 130 319 toxin ETX/Bacillus mosquitocidal toxin MTX2APG00887.1 13 Alternate PF03318 Clostridium epsilon 114 303 start toxinETX/Bacillus mosquitocidal toxin MTX2 APG00961.0 14 PF03945 deltaendotoxin, N- 67 263 terminal domain PF00555 delta endotoxin 271 494PF03944 delta endotoxin 505 639 APG00961.1 15 Alternate PF03945 deltaendotoxin, N- 67 263 start terminal domain PF00555 delta endotoxin 271494 PF03944 delta endotoxin 505 639 APG00973.0 16 no PFAM domainsAPG00973.1 17 Alternate no PFAM start domains APG00973.2 18 Alternate noPFAM start domains APG01038.0 19 PF03318 Clostridium epsilon 84 189toxin ETX/Bacillus mosquitocidal toxin MTX2 APG01038.1 20 Signal PF03318Clostridium epsilon 58 161 peptide toxin ETX/Bacillus removedmosquitocidal toxin MTX2 APG01063.0 21 PF14200 Ricin-type beta-trefoil33 109 lectin domain-like PF05431 Insecticidal Crystal 241 401 Toxin,P42 APG01063.1 22 Signal PF14200 Ricin-type beta-trefoil 6 82 peptidelectin domain-like removed PF05431 Insecticidal Crystal 214 374 Toxin,P42 APG01092.0 23 PF03945 delta endotoxin, N- 243 338 terminal domainPF03944 delta endotoxin 544 681 APG01092.1 24 3′ Truncation PF03945delta endotoxin, N- 243 338 terminal domain PF03944 delta endotoxin 544680 APG01102.0 25 PF03944 delta endotoxin 563 721 APG01102.1 26 3′Truncation PF03944 delta endotoxin 563 721 APG01102 27 no PFAM SplitCrydomains (APG01056.0) APG01127.0 28 PF03318 Clostridium epsilon 44 249toxin ETX/Bacillus mosquitocidal toxin MTX2 APG01141.0 29 PF14200Ricin-type beta-trefoil 36 109 lectin domain-like PF05431 InsecticidalCrystal 181 336 Toxin, P42 APG01141.1 30 Alternate PF14200 Ricin-typebeta-trefoil 36 109 start lectin domain-like PF05431 InsecticidalCrystal 181 336 Toxin, P42 APG01163.0 31 PF03318 Clostridium epsilon 123215 toxin ETX/Bacillus mosquitocidal toxin MTX2 APG01163.1 32 SignalPF03318 Clostridium epsilon 94 186 peptide toxin ETX/Bacillus removedmosquitocidal toxin MTX2 APG01165.0 33 PF03945 delta endotoxin, N- 54127 terminal domain PF03945 delta endotoxin, N- 212 287 terminal domainAPG01165.1 34 Alternate PF03945 delta endotoxin, N- 52 125 startterminal domain PF03945 delta endotoxin, N- 210 285 terminal domainAPG01201.0 35 PF03318 Clostridium epsilon 131 322 toxin ETX/Bacillusmosquitocidal toxin MTX2 APG01201.1 36 Signal PF03318 Clostridiumepsilon 104 295 peptide toxin ETX/Bacillus removed mosquitocidal toxinMTX2 APG01281.0 37 PF03318 Clostridium epsilon 136 244 toxinETX/Bacillus mosquitocidal toxin MTX2 APG01281.1 38 Signal PF03318Clostridium epsilon 107 215 peptide toxin ETX/Bacillus removedmosquitocidal toxin MTX2 APG01352.0 39 PF05431 Insecticidal Crystal 206372 Toxin, P42 APG01352.1 40 Signal PF05431 Insecticidal Crystal 179 345peptide Toxin, P42 removed APG01380.0 41 no PFAM domains APG01385.0 42PF03318 Clostridium epsilon 33 274 toxin ETX/Bacillus mosquitocidaltoxin MTX2 APG01396.0 43 PF03318 Clostridium epsilon 51 285 toxinETX/Bacillus mosquitocidal toxin MTX2 APG01396.1 44 Alternate PF03318Clostridium epsilon 29 263 start toxin ETX/Bacillus mosquitocidal toxinMTX2 APG01434.0 45 PF03945 delta endotoxin, N- 115 302 terminal domainPF00555 delta endotoxin 315 485 PF03944 delta endotoxin 498 636APG01434.1 46 Alternate PF03945 delta endotoxin, N- 112 299 startterminal domain PF00555 delta endotoxin 312 482 PF03944 delta endotoxin495 633 APG01434.2 47 Alternate PF03945 delta endotoxin, N- 112 299start and 3′ terminal domain Truncation PF00555 delta endotoxin 312 482PF03944 delta endotoxin 495 632 APG01434 48 no PFAM SplitCry domains(APG03109.0) APG01455.0 49 no PFAM domains APG01516.0 50 PF03318Clostridium epsilon 129 241 toxin ETX/Bacillus mosquitocidal toxin MTX2APG01516.1 51 Signal PF03318 Clostridium epsilon 93 205 peptide toxinETX/Bacillus removed mosquitocidal toxin MTX2 APG01549.0 52 PF03318Clostridium epsilon 62 270 toxin ETX/Bacillus mosquitocidal toxin MTX2APG01617.0 53 PF03318 Clostridium epsilon 103 313 toxin ETX/Bacillusmosquitocidal toxin MTX2 APG01617.1 54 Signal PF03318 Clostridiumepsilon 72 282 peptide toxin ETX/Bacillus removed mosquitocidal toxinMTX2 APG01681.0 55 PF14200 Ricin-type beta-trefoil 80 141 lectindomain-like PF05431 Insecticidal Crystal 240 404 Toxin, P42 APG01681.156 Alternate PF14200 Ricin-type beta-trefoil 64 125 start lectindomain-like PF05431 Insecticidal Crystal 224 388 Toxin, P42 APG01681 57no PFAM CryBP1 domains (APG08871.0) APG01686.0 58 PF03945 deltaendotoxin, N- 244 338 terminal domain PF03944 delta endotoxin 544 679APG01686.1 59 3′ Truncation PF03945 delta endotoxin, N- 244 338 terminaldomain PF03944 delta endotoxin 544 678 APG01710.0 60 PF03945 deltaendotoxin, N- 123 330 terminal domain APG01710.1 61 Alternate PF03945delta endotoxin, N- 54 261 start terminal domain APG01788.0 62 PF03945delta endotoxin, N- 106 323 terminal domain PF03944 delta endotoxin 383510 APG01788.1 63 3′ Truncation PF03945 delta endotoxin, N- 106 323terminal domain PF03944 delta endotoxin 383 510 APG01803.0 64 PF01473Putative cell wall 411 427 binding repeat PF01473 Putative cell wall 439452 binding repeat APG01803.1 65 Signal PF01473 Putative cell wall 373389 peptide binding repeat removed PF01473 Putative cell wall 401 414binding repeat APG01810.0 66 PF14200 Ricin-type beta-trefoil 32 99lectin domain-like PF05431 Insecticidal Crystal 223 383 Toxin, P42APG01810.1 67 Signal PF14200 Ricin-type beta-trefoil 5 72 peptide lectindomain-like removed PF05431 Insecticidal Crystal 196 356 Toxin, P42APG01817.0 68 PF03945 delta endotoxin, N- 89 292 terminal domain PF00555delta endotoxin 302 509 PF03944 delta endotoxin 520 666 APG01819.0 69PF03945 delta endotoxin, N- 113 310 terminal domain PF01473 Putativecell wall 388 405 binding repeat PF01473 Putative cell wall 408 424binding repeat APG01819.1 70 Signal PF03945 delta endotoxin, N- 71 267peptide terminal domain removed PF01473 Putative cell wall 346 363binding repeat PF01473 Putative cell wall 366 382 binding repeatAPG01860.0 71 PF03945 delta endotoxin, N- 116 316 terminal domainAPG01860.1 72 Signal PF03945 delta endotoxin, N- 78 278 peptide terminaldomain removed APG01967.0 73 PF03318 Clostridium epsilon 48 258 toxinETX/Bacillus mosquitocidal toxin MTX2 APG01967.1 74 Alternate PF03318Clostridium epsilon 44 255 start toxin ETX/Bacillus mosquitocidal toxinMTX2 APG02071.0 75 PF03945 delta endotoxin, N- 95 305 terminal domainPF03944 delta endotoxin 505 643 APG02071.1 76 Alternate PF03945 deltaendotoxin, N- 82 292 start and 3′ terminal domain Truncation PF03944delta endotoxin 492 630 APG02105.0 77 PF03318 Clostridium epsilon 45 280toxin ETX/Bacillus mosquitocidal toxin MTX2 APG02110.0 78 PF03945 deltaendotoxin, N- 143 323 terminal domain PF14200 Ricin-type beta-trefoil464 549 lectin domain-like APG02110.1 79 Alternate PF03945 deltaendotoxin, N- 120 293 start terminal domain PF14200 Ricin-typebeta-trefoil 441 526 lectin domain-like APG02115.0 80 PF03318Clostridium epsilon 34 189 toxin ETX/Bacillus mosquitocidal toxin MTX2APG02143.0 81 PF03318 Clostridium epsilon 32 278 toxin ETX/Bacillusmosquitocidal toxin MTX2 APG02166.0 82 PF03318 Clostridium epsilon 59224 toxin ETX/Bacillus mosquitocidal toxin MTX2 APG02206.0 83 no PFAMdomains APG02206.1 84 Alternate no PFAM start domains APG02206 85PF07029 CryBP1 protein 40 146 SplitCry (APG07749.0) APG02221.0 86PF03945 delta endotoxin, N- 225 334 terminal domain PF03944 deltaendotoxin 562 699 APG02221.1 87 3′ Truncation PF03945 delta endotoxin,N- 225 334 terminal domain PF03944 delta endotoxin 562 699 APG02256.0 88PF03318 Clostridium epsilon 68 209 toxin ETX/Bacillus mosquitocidaltoxin MTX2 APG02256.1 89 Signal PF03318 Clostridium epsilon 40 181peptide toxin ETX/Bacillus removed mosquitocidal toxin MTX2 APG02309.090 PF03945 delta endotoxin, N- 87 304 terminal domain PF03944 deltaendotoxin 525 648 APG02309.1 91 Alternate PF03945 delta endotoxin, N- 83300 start terminal domain PF03944 delta endotoxin 521 644 APG02309.2 92Alternate PF03945 delta endotoxin, N- 83 300 start and 3′ terminaldomain Truncation PF03944 delta endotoxin 521 643 APG02315.0 93 PF03318Clostridium epsilon 81 289 toxin ETX/Bacillus mosquitocidal toxin MTX2APG02315.1 94 Signal PF03318 Clostridium epsilon 58 266 peptide toxinETX/Bacillus removed mosquitocidal toxin MTX2 APG02319.0 95 no PFAMdomains APG02380.0 96 PF12495 Vegetative insecticide 14 190 protein 3A Nterminal PF02018 Carbohydrate binding 538 654 domain APG02380.1 97Alternate PF12495 Vegetative insecticide 12 188 start protein 3A Nterminal PF02018 Carbohydrate binding 536 652 domain APG02451.0 98PF03318 Clostridium epsilon 39 274 toxin ETX/Bacillus mosquitocidaltoxin MTX2 APG02451.1 99 Alternate PF03318 Clostridium epsilon 33 268start toxin ETX/Bacillus mosquitocidal toxin MTX2 APG02475.0 100 PF03318Clostridium epsilon 179 373 toxin ETX/Bacillus mosquitocidal toxin MTX2APG02475.1 101 Alternate PF03318 Clostridium epsilon 164 358 start toxinETX/Bacillus mosquitocidal toxin MTX2 APG02475.2 102 Signal PF03318Clostridium epsilon 134 328 peptide toxin ETX/Bacillus removedmosquitocidal toxin MTX2 APG02506.0 103 PF03945 delta endotoxin, N- 130317 terminal domain PF01473 Putative cell wall 465 476 binding repeatPF01473 Putative cell wall 550 560 binding repeat APG02506.1 104 SignalPF03945 delta endotoxin, N- 92 279 peptide terminal domain removedPF01473 Putative cell wall 427 438 binding repeat PF01473 Putative cellwall 512 522 binding repeat APG02511.0 105 no PFAM domains APG02511.1106 Alternate no PFAM start domains APG02511.2 107 Signal no PFAMpeptide domains removed APG02587.0 108 PF00555 delta endotoxin 320 515PF03944 delta endotoxin 537 679 APG02587.1 109 3′ Truncation PF03945delta endotoxin, N- 74 310 terminal domain PF00555 delta endotoxin 320515 PF03944 delta endotoxin 537 679 APG02592.0 110 PF03318 Clostridiumepsilon 53 267 toxin ETX/Bacillus mosquitocidal toxin MTX2 APG02656.0111 no PFAM domains APG02656.1 112 Alternate no PFAM start domainsAPG02659.0 113 no PFAM domains APG02659.1 114 Signal no PFAM peptidedomains removed APG02671.0 115 PF01338 Bacillus thuringiensis 39 254toxin APG02671.1 116 Alternate PF01338 Bacillus thuringiensis 23 238start toxin APG02676.0 117 no PFAM domains APG02706.0 118 PF03945 deltaendotoxin, N- 73 293 terminal domain PF03944 delta endotoxin 517 656APG02706.1 119 Alternate PF03945 delta endotoxin, N- 73 293 startterminal domain PF03944 delta endotoxin 517 656 APG02706.2 120 AlternatePF03945 delta endotoxin, N- 73 293 start and 3′ terminal domainTruncation PF03944 delta endotoxin 517 656 APG02706.3 121 AlternatePF03945 delta endotoxin, N- 64 284 start terminal domain PF03944 deltaendotoxin 508 647 APG02706.4 122 Alternate PF03945 delta endotoxin, N-64 284 start and 3′ terminal domain Truncation PF03944 delta endotoxin508 647 APG02706.5 123 Alternate PF03945 delta endotoxin, N- 16 226start terminal domain PF03944 delta endotoxin 450 589 APG02706.6 124Alternate PF03945 delta endotoxin, N- 16 226 start and 3′ terminaldomain Truncation PF03944 delta endotoxin 450 589 APG02714.0 125 PF03318Clostridium epsilon 60 206 toxin ETX/Bacillus mosquitocidal toxin MTX2APG02718.0 126 PF06101 Vacuolar protein 13 234 sorting-associatedprotein 62 PF05431 Insecticidal Crystal 267 408 Toxin, P42 APG02718.1127 Alternate PF06101 Vacuolar protein 3 221 start sorting-associatedprotein 62 PF05431 Insecticidal Crystal 254 395 Toxin, P42 APG02767.0128 PF12495 Vegetative insecticide 16 187 protein 3A N terminal PF02018Carbohydrate binding 547 659 domain APG02767.1 129 Alternate PF12495Vegetative insecticide 14 185 start protein 3A N terminal PF02018Carbohydrate binding 545 657 domain APG02941.0 130 no PFAM domainsAPG02947.0 131 PF03945 delta endotoxin, N- 89 302 terminal domainPF00555 delta endotoxin 310 520 PF03944 delta endotoxin 531 659 PF14200Ricin-type beta-trefoil 700 789 lectin domain-like APG02947.1 132Alternate PF03945 delta endotoxin, N- 73 286 start terminal domainPF00555 delta endotoxin 294 504 PF03944 delta endotoxin 515 643 PF14200Ricin-type beta-trefoil 684 773 lectin domain-like APG02947.2 133Alternate PF03945 delta endotoxin, N- 73 286 start and 3′ terminaldomain Truncation PF00555 delta endotoxin 294 504 PF03944 deltaendotoxin 515 643 APG02956.0 134 PF03318 Clostridium epsilon 70 270toxin ETX/Bacillus mosquitocidal toxin MTX2 APG02986.0 135 PF03945 deltaendotoxin, N- 70 245 terminal domain PF00555 delta endotoxin 342 547PF03944 delta endotoxin 557 691 APG03018.0 136 PF03945 delta endotoxin,N- 113 297 terminal domain PF00555 delta endotoxin 311 484 PF03944 deltaendotoxin 495 636 APG03018.1 137 Alternate PF03945 delta endotoxin, N-110 294 start terminal domain PF00555 delta endotoxin 308 481 PF03944delta endotoxin 492 633 APG03018.2 138 Alternate PF03945 deltaendotoxin, N- 110 294 start and 3′ terminal domain Truncation PF00555delta endotoxin 308 481 PF03944 delta endotoxin 492 632 APG03018 139 noPFAM SplitCry domains (APG08753.0) APG03054.0 140 PF03945 deltaendotoxin, N- 87 287 terminal domain PF00555 delta endotoxin 295 511PF03944 delta endotoxin 521 658 APG03056.0 141 PF03945 delta endotoxin,N- 82 290 terminal domain PF00555 delta endotoxin 299 504 PF03944 deltaendotoxin 515 650 APG03056.1 142 3′ Truncation PF03945 delta endotoxin,N- 82 290 terminal domain PF00555 delta endotoxin 299 504 PF03944 deltaendotoxin 515 649 APG03056 143 no PFAM CryBP1 domains (APG02727.0)APG03182.0 144 no PFAM domains APG03182.1 145 Alternate no PFAM startdomains APG03182.2 146 Signal no PFAM peptide domains removed APG03218.0147 PF03318 Clostridium epsilon 121 312 toxin ETX/Bacillus mosquitocidaltoxin MTX2 APG03218.1 148 Signal PF03318 Clostridium epsilon 95 286peptide toxin ETX/Bacillus removed mosquitocidal toxin MTX2 APG03247.0149 PF03945 delta endotoxin, N- 124 329 terminal domain PF03944 deltaendotoxin 519 662 APG03247.1 150 Alternate PF03945 delta endotoxin, N-107 312 start and 3′ terminal domain Truncation PF03944 delta endotoxin502 645 APG03361.0 151 PF05431 Insecticidal Crystal 300 468 Toxin, P42APG03392.0 152 PF03945 delta endotoxin, N- 122 306 terminal domainPF01473 Putative cell wall 437 449 binding repeat APG03392.1 153 SignalPF03945 delta endotoxin, N- 84 268 peptide terminal domain removedPF01473 Putative cell wall 399 411 binding repeat APG03415.0 154 PF03318Clostridium epsilon 40 282 toxin ETX/Bacillus mosquitocidal toxin MTX2APG03504.0 155 PF03318 Clostridium epsilon 45 248 toxin ETX/Bacillusmosquitocidal toxin MTX2 APG03505.0 156 PF03945 delta endotoxin, N- 78299 terminal domain PF03944 delta endotoxin 490 648 APG03505.1 157Alternate PF03945 delta endotoxin, N- 75 296 start terminal domainPF03944 delta endotoxin 487 645 APG03505.2 158 Alternate PF03945 deltaendotoxin, N- 75 296 start and 3′ terminal domain Truncation PF03944delta endotoxin 487 645 APG03505.3 159 Alternate PF03945 deltaendotoxin, N- 36 257 start terminal domain PF03944 delta endotoxin 448606 APG03505.4 160 Alternate PF03945 delta endotoxin, N- 36 257 startand 3′ terminal domain Truncation PF03944 delta endotoxin 448 606APG03505.5 161 Alternate PF03945 delta endotoxin, N- 17 238 startterminal domain PF03944 delta endotoxin 429 587 APG03505.6 162 AlternatePF03945 delta endotoxin, N- 17 238 start and 3′ terminal domainTruncation PF03944 delta endotoxin 429 587 APG03527.0 163 PF03318Clostridium epsilon 61 251 toxin ETX/Bacillus mosquitocidal toxin MTX2APG03527.1 164 Alternate PF03318 Clostridium epsilon 58 248 start toxinETX/Bacillus mosquitocidal toxin MTX2 APG03584.0 165 no PFAM domainsAPG03584.1 166 Signal no PFAM peptide domains removed APG03587.0 167 noPFAM domains APG03674.0 168 PF01338 Bacillus thuringiensis 5 192 toxinAPG03674.1 169 Alternate PF01338 Bacillus thuringiensis 4 192 starttoxin APG03738.0 170 PF03318 Clostridium epsilon 46 206 toxinETX/Bacillus mosquitocidal toxin MTX2 APG03748.0 171 PF14200 Ricin-typebeta-trefoil 81 157 lectin domain-like PF05431 Insecticidal Crystal 238398 Toxin, P42 APG03748.1 172 Signal PF14200 Ricin-type beta-trefoil 46121 peptide lectin domain-like removed PF05431 Insecticidal Crystal 202362 Toxin, P42 APG03758.0 173 PF03318 Clostridium epsilon 42 269 toxinETX/Bacillus mosquitocidal toxin MTX2 PF00388 Phosphatidylinositol- 358469 specific phospholipase C, X domain APG03778.0 174 PF05431Insecticidal Crystal 312 475 Toxin, P42 APG03789.0 175 PF05431Insecticidal Crystal 223 377 Toxin, P42 APG03826.0 176 PF03318Clostridium epsilon 70 245 toxin ETX/Bacillus mosquitocidal toxin MTX2APG03887.0 177 no PFAM domains APG03893.0 178 PF03945 delta endotoxin,N- 85 289 terminal domain PF00555 delta endotoxin 299 503 PF03944 deltaendotoxin 514 645 APG03909.0 179 PF03945 delta endotoxin, N- 158 307terminal domain APG03909.1 180 Alternate PF03945 delta endotoxin, N- 125274 start terminal domain APG03959.0 181 PF01338 Bacillus thuringiensis4 205 toxin APG04009.0 182 no PFAM domains APG04019.0 183 PF05431Insecticidal Crystal 89 254 Toxin, P42 APG04019.1 184 Alternate PF05431Insecticidal Crystal 89 254 start Toxin, P42 APG04103.0 185 PF03318Clostridium epsilon 115 318 toxin ETX/Bacillus mosquitocidal toxin MTX2APG04103.1 186 Signal PF03318 Clostridium epsilon 89 292 peptide toxinETX/Bacillus removed mosquitocidal toxin MTX2 APG04111.0 187 PF03318Clostridium epsilon 52 253 toxin ETX/Bacillus mosquitocidal toxin MTX2APG04141.0 188 PF03945 delta endotoxin, N- 19 172 terminal domainAPG04162.0 189 no PFAM domains APG04162.1 190 Signal no PFAM peptidedomains removed APG04208.0 191 PF14200 Ricin-type beta-trefoil 31 101lectin domain-like PF05431 Insecticidal Crystal 239 397 Toxin, P42APG04208.1 192 Signal PF14200 Ricin-type beta-trefoil 5 73 peptidelectin domain-like removed PF05431 Insecticidal Crystal 212 370 Toxin,P42 APG04302.0 193 PF14200 Ricin-type beta-trefoil 35 91 lectindomain-like PF05431 Insecticidal Crystal 197 358 Toxin, P42 APG04316.0194 no PFAM domains APG04316.1 195 Signal no PFAM peptide domainsremoved APG04323.0 196 no PFAM domains APG04360.0 197 PF01338 Bacillusthuringiensis 19 202 toxin APG04439.0 198 no PFAM domains APG04439.1 199Alternate no PFAM start domains APG04442.0 200 no PFAM domainsAPG04442.1 201 Signal no PFAM peptide domains removed APG04476.0 202PF03945 delta endotoxin, N- 78 308 terminal domain PF00030 Beta/Gamma745 824 crystallin APG04476.1 203 Alternate PF03945 delta endotoxin, N-64 294 start terminal domain PF00030 Beta/Gamma 731 810 crystallinAPG04568.0 204 PF14200 Ricin-type beta-trefoil 31 102 lectin domain-likePF05431 Insecticidal Crystal 233 393 Toxin, P42 APG04568.1 205 SignalPF14200 Ricin-type beta-trefoil 4 73 peptide lectin domain-like removedPF05431 Insecticidal Crystal 204 364 Toxin, P42 APG04602.0 206 PF07691PA14 domain 46 174 PF03495 Clostridial binary toxin 213 288 B/anthraxtoxin PA Ca- binding domain PF17475 Clostridial binary toxin 291 500B/anthrax toxin PA domain 2 PF17476 Clostridial binary toxin 519 607B/anthrax toxin PA domain 3 APG04602.1 207 Signal PF07691 PA14 domain 17145 peptide PF03495 Clostridial binary toxin 184 259 removed B/anthraxtoxin PA Ca- binding domain PF17475 Clostridial binary toxin 262 471B/anthrax toxin PA domain 2 PF17476 Clostridial binary toxin 490 578B/anthrax toxin PA domain 3 APG04651.0 208 PF03945 delta endotoxin, N-76 279 terminal domain PF00555 delta endotoxin 287 499 PF03944 deltaendotoxin 509 646 APG04683.0 209 no PFAM domains APG04683.1 210Alternate no PFAM start domains APG04683.2 211 Signal no PFAM peptidedomains removed APG04687.0 212 PF03318 Clostridium epsilon 129 220 toxinETX/Bacillus mosquitocidal toxin MTX2 APG04687.1 213 Alternate PF03318Clostridium epsilon 127 219 start toxin ETX/Bacillus mosquitocidal toxinMTX2 APG04687.2 214 Signal PF03318 Clostridium epsilon 98 189 peptidetoxin ETX/Bacillus removed mosquitocidal toxin MTX2 APG04692.0 215PF03318 Clostridium epsilon 108 265 toxin ETX/Bacillus mosquitocidaltoxin MTX2 APG04692.1 216 Alternate PF03318 Clostridium epsilon 103 260start toxin ETX/Bacillus mosquitocidal toxin MTX2 APG04692.2 217 SignalPF03318 Clostridium epsilon 75 232 peptide toxin ETX/Bacillus removedmosquitocidal toxin MTX2 APG04694.0 218 PF03945 delta endotoxin, N- 92263 terminal domain PF00555 delta endotoxin 349 559 PF03944 deltaendotoxin 569 698 APG04694.1 219 3′ Truncation PF03945 delta endotoxin,N- 92 263 terminal domain PF00555 delta endotoxin 349 559 PF03944 deltaendotoxin 569 698 APG04694 220 no PFAM CryBP1 domains (APG06866.0)APG04786.0 221 PF03945 delta endotoxin, N- 83 267 terminal domainPF00555 delta endotoxin 326 509 PF03944 delta endotoxin 520 650 PF14200Ricin-type beta-trefoil 697 784 lectin domain-like APG04786.1 222Alternate PF03945 delta endotoxin, N- 81 265 start terminal domainPF00555 delta endotoxin 324 507 PF03944 delta endotoxin 518 648 PF14200Ricin-type beta-trefoil 695 782 lectin domain-like APG04786.2 223Alternate PF03945 delta endotoxin, N- 81 265 start and 3′ terminaldomain Truncation PF00555 delta endotoxin 324 507 PF03944 deltaendotoxin 518 648 APG04794.0 224 no PFAM domains APG04821.0 225 PF03318Clostridium epsilon 44 281 toxin ETX/Bacillus mosquitocidal toxin MTX2APG04821.1 226 Alternate PF03318 Clostridium epsilon 32 269 start toxinETX/Bacillus mosquitocidal toxin MTX2 APG04822.0 227 PF03318 Clostridiumepsilon 84 319 toxin ETX/Bacillus mosquitocidal toxin MTX2 APG04822.1228 Signal PF03318 Clostridium epsilon 71 306 peptide toxin ETX/Bacillusremoved mosquitocidal toxin MTX2 APG04959.0 229 PF03945 delta endotoxin,N- 118 300 terminal domain PF00555 delta endotoxin 310 507 PF03944 deltaendotoxin 518 659 APG04966.0 230 PF14200 Ricin-type beta-trefoil 72 153lectin domain-like PF05431 Insecticidal Crystal 224 385 Toxin, P42APG04966.1 231 Signal PF14200 Ricin-type beta-trefoil 44 126 peptidelectin domain-like removed PF05431 Insecticidal Crystal 196 357 Toxin,P42 APG04977.0 232 PF03318 Clostridium epsilon 50 241 toxin ETX/Bacillusmosquitocidal toxin MTX2 APG05073.0 233 PF03945 delta endotoxin, N- 89302 terminal domain PF03944 delta endotoxin 523 669 APG05073.1 234Signal PF03945 delta endotoxin, N- 53 266 peptide terminal domainremoved PF03944 delta endotoxin 487 633 APG05073.2 235 Signal PF03945delta endotoxin, N- 53 266 peptide terminal domain removed and PF03944delta endotoxin 487 633 3′ Truncation APG05078.0 236 no PFAM domainsAPG05095.0 237 PF03945 delta endotoxin, N- 90 267 terminal domainAPG05095.1 238 Alternate PF03945 delta endotoxin, N- 90 267 startterminal domain APG05115.0 239 PF01823 MAC/Perforin domain 143 335APG05149.0 240 no PFAM domains APG05149.1 241 Signal no PFAM peptidedomains removed APG05279.0 242 PF03945 delta endotoxin, N- 152 301terminal domain APG05279.1 243 Alternate PF03945 delta endotoxin, N- 126275 start terminal domain APG05279.2 244 Signal PF03945 delta endotoxin,N- 85 234 peptide terminal domain removed APG05300.0 245 no PFAM domainsAPG05346.0 246 PF03318 Clostridium epsilon 164 357 toxin ETX/Bacillusmosquitocidal toxin MTX2 APG05346.1 247 Signal PF03318 Clostridiumepsilon 134 328 peptide toxin ETX/Bacillus removed mosquitocidal toxinMTX2 APG05388.0 248 PF03318 Clostridium epsilon 24 239 toxinETX/Bacillus mosquitocidal toxin MTX2 APG05408.0 249 no PFAM domainsAPG05408.1 250 Alternate no PFAM start domains APG05414.0 251 PF03318Clostridium epsilon 75 211 toxin ETX/Bacillus mosquitocidal toxin MTX2APG05414.1 252 Alternate PF03318 Clostridium epsilon 75 211 start toxinETX/Bacillus mosquitocidal toxin MTX2 APG05429.0 253 PF03318 Clostridiumepsilon 91 229 toxin ETX/Bacillus mosquitocidal toxin MTX2 APG05429.1254 Alternate PF03318 Clostridium epsilon 74 213 start toxinETX/Bacillus mosquitocidal toxin MTX2 APG05429.2 255 Signal PF03318Clostridium epsilon 42 181 peptide toxin ETX/Bacillus removedmosquitocidal toxin MTX2 APG05431.0 256 no PFAM domains APG05431.1 257Signal no PFAM peptide domains removed APG05471.0 258 PF01823MAC/Perforin domain 135 332 APG05481.0 259 PF03318 Clostridium epsilon153 314 toxin ETX/Bacillus mosquitocidal toxin MTX2 APG05481.1 260Alternate PF03318 Clostridium epsilon 140 301 start toxin ETX/Bacillusmosquitocidal toxin MTX2 APG05481.2 261 Signal PF03318 Clostridiumepsilon 111 272 peptide toxin ETX/Bacillus removed mosquitocidal toxinMTX2 APG05492.0 262 PF03318 Clostridium epsilon 47 257 toxinETX/Bacillus mosquitocidal toxin MTX2 APG05508.0 263 PF03945 deltaendotoxin, N- 67 297 terminal domain PF00030 Beta/Gamma 735 812crystallin PF14200 Ricin-type beta-trefoil 900 943 lectin domain-likeAPG05508.1 264 Alternate PF03945 delta endotoxin, N- 64 294 startterminal domain PF00030 Beta/Gamma 732 809 crystallin PF14200 Ricin-typebeta-trefoil 897 940 lectin domain-like APG05538.0 265 PF03945 deltaendotoxin, N- 118 290 terminal domain PF14200 Ricin-type beta-trefoil502 586 lectin domain-like APG05538.1 266 Alternate PF03945 deltaendotoxin, N- 116 288 start terminal domain PF14200 Ricin-typebeta-trefoil 500 584 lectin domain-like APG05538.2 267 Signal PF03945delta endotoxin, N- 78 250 peptide terminal domain removed PF14200Ricin-type beta-trefoil 462 546 lectin domain-like APG05636.0 268PF03318 Clostridium epsilon 46 248 toxin ETX/Bacillus mosquitocidaltoxin MTX2 APG05643.0 269 PF03944 delta endotoxin 596 734 APG05643.1 270Alternate PF03944 delta endotoxin 596 734 start and 3′ TruncationAPG05643 271 no PFAM SplitCry domains (APG08971.0) APG05662.0 272PF01338 Bacillus thuringiensis 4 207 toxin APG05725.0 273 PF03945 deltaendotoxin, N- 49 243 terminal domain PF01473 Putative cell wall 360 374binding repeat APG05725.1 274 Alternate PF03945 delta endotoxin, N- 49243 start terminal domain PF01473 Putative cell wall 360 374 bindingrepeat APG05734.0 275 PF03945 delta endotoxin, N- 124 354 terminaldomain PF03944 delta endotoxin 550 686 APG05734.1 276 Alternate PF03945delta endotoxin, N- 103 333 start terminal domain PF03944 deltaendotoxin 529 665 APG05734.2 277 Signal PF03945 delta endotoxin, N- 73303 peptide terminal domain removed PF03944 delta endotoxin 499 635APG05734.3 278 Alternate PF03945 delta endotoxin, N- 103 333 start and3′ terminal domain Truncation PF03944 delta endotoxin 529 665 APG05734.4279 Signal PF03945 delta endotoxin, N- 73 303 peptide terminal domainremoved and PF03944 delta endotoxin 499 635 3′ Truncation APG05793.0 280PF03318 Clostridium epsilon 16 161 toxin ETX/Bacillus mosquitocidaltoxin MTX2 APG05834.0 281 PF03318 Clostridium epsilon 103 321 toxinETX/Bacillus mosquitocidal toxin MTX2 APG05834.1 282 Signal PF03318Clostridium epsilon 70 288 peptide toxin ETX/Bacillus removedmosquitocidal toxin MTX2 APG05878.0 283 PF03945 delta endotoxin, N- 79254 terminal domain APG05907.0 284 PF05431 Insecticidal Crystal 245 406Toxin, P42 APG05907.1 285 Alternate PF05431 Insecticidal Crystal 216 377start Toxin, P42 APG05917.0 286 PF05431 Insecticidal Crystal 217 378Toxin, P42 APG05920.0 287 no PFAM domains APG05920.1 288 Signal no PFAMpeptide domains removed APG05946.0 289 PF14200 Ricin-type beta-trefoil 653 lectin domain-like PF05431 Insecticidal Crystal 90 252 Toxin, P42APG05955.0 290 PF05431 Insecticidal Crystal 330 495 Toxin, P42APG05992.0 291 no PFAM domains APG06027.0 292 PF14200 Ricin-typebeta-trefoil 41 101 lectin domain-like PF05431 Insecticidal Crystal 212372 Toxin, P42 APG06027.1 293 Alternate PF14200 Ricin-type beta-trefoil41 101 start lectin domain-like PF05431 Insecticidal Crystal 212 372Toxin, P42 APG06049.0 294 PF03318 Clostridium epsilon 144 341 toxinETX/Bacillus mosquitocidal toxin MTX2 APG06049.1 295 Signal PF03318Clostridium epsilon 114 312 peptide toxin ETX/Bacillus removedmosquitocidal toxin MTX2 APG06079.0 296 PF03945 delta endotoxin, N- 86312 terminal domain PF00555 delta endotoxin 322 485 PF03944 deltaendotoxin 518 656 PF05588 Clostridium botulinum 668 752 HA-17 domainAPG06079.1 297 3′ Truncation PF03945 delta endotoxin, N- 86 312 terminaldomain PF00555 delta endotoxin 322 485 PF03944 delta endotoxin 518 656APG06195.0 298 PF03318 Clostridium epsilon 65 280 toxin ETX/Bacillusmosquitocidal toxin MTX2 APG06213.0 299 PF03945 delta endotoxin, N- 143322 terminal domain PF14200 Ricin-type beta-trefoil 464 549 lectindomain-like APG06213.1 300 Alternate PF03945 delta endotoxin, N- 120 299start terminal domain PF14200 Ricin-type beta-trefoil 441 526 lectindomain-like APG06213.2 301 Signal PF03945 delta endotoxin, N- 84 263peptide terminal domain removed PF14200 Ricin-type beta-trefoil 405 490lectin domain-like APG06235.0 302 PF12495 Vegetative insecticide 10 185protein 3A N terminal APG06283.0 303 no PFAM domains APG06304.0 304 noPFAM domains APG06304.1 305 Signal no PFAM peptide domains removedAPG06319.0 306 PF03318 Clostridium epsilon 143 341 toxin ETX/Bacillusmosquitocidal toxin MTX2 APG06319.1 307 Signal PF03318 Clostridiumepsilon 114 312 peptide toxin ETX/Bacillus removed mosquitocidal toxinMTX2 APG06331.0 308 PF14200 Ricin-type beta-trefoil 31 101 lectindomain-like PF05431 Insecticidal Crystal 236 396 Toxin, P42 APG06331.1309 Signal PF14200 Ricin-type beta-trefoil 5 74 peptide lectindomain-like removed PF05431 Insecticidal Crystal 209 369 Toxin, P42APG06350.0 310 PF00652 Ricin-type beta-trefoil 52 180 lectin domainAPG06350.1 311 Signal PF00652 Ricin-type beta-trefoil 17 145 peptidelectin domain removed APG06378.0 312 PF02018 Carbohydrate binding 562679 domain PF03496 ADP- 937 1130 ribosyltransferase exoenzyme APG06424.0313 PF03945 delta endotoxin, N- 60 237 terminal domain APG06479.0 314 noPFAM domains APG06479.1 315 Signal no PFAM peptide domains removedAPG06480.0 316 PF03318 Clostridium epsilon 128 316 toxin ETX/Bacillusmosquitocidal toxin MTX2 APG06480.1 317 Alternate PF03318 Clostridiumepsilon 80 268 start toxin ETX/Bacillus mosquitocidal toxin MTX2APG06493.0 318 PF03945 delta endotoxin, N- 148 334 terminal domainAPG06493.1 319 Alternate PF03945 delta endotoxin, N- 82 268 startterminal domain APG06496.0 320 PF01338 Bacillus thuringiensis 22 244toxin APG06559.0 321 PF14200 Ricin-type beta-trefoil 81 161 lectindomain-like PF05431 Insecticidal Crystal 215 378 Toxin, P42 APG06576.0322 PF03945 delta endotoxin, N- 90 271 terminal domain PF00555 deltaendotoxin 329 413 PF03944 delta endotoxin 505 639 APG06576.1 323 3′Truncation PF03945 delta endotoxin, N- 90 271 terminal domain PF00555delta endotoxin 329 413 PF03944 delta endotoxin 505 638 APG06578.0 324PF03318 Clostridium epsilon 63 240 toxin ETX/Bacillus mosquitocidaltoxin MTX2 APG06606.0 325 PF03945 delta endotoxin, N- 143 315 terminaldomain PF14200 Ricin-type beta-trefoil 464 549 lectin domain-likeAPG06606.1 326 Alternate PF03945 delta endotoxin, N- 120 292 startterminal domain PF14200 Ricin-type beta-trefoil 441 526 lectindomain-like APG06606.2 327 Signal PF03945 delta endotoxin, N- 84 256peptide terminal domain removed PF14200 Ricin-type beta-trefoil 405 490lectin domain-like APG06677.0 328 PF03945 delta endotoxin, N- 3 87terminal domain PF00555 delta endotoxin 95 313 PF03944 delta endotoxin323 461 APG06677.1 329 Alternate PF03945 delta endotoxin, N- 3 87 startand 3′ terminal domain Truncation PF00555 delta endotoxin 95 313 PF03944delta endotoxin 323 461 APG06697.0 330 PF14200 Ricin-type beta-trefoil76 149 lectin domain-like PF05431 Insecticidal Crystal 269 428 Toxin,P42 APG06697.1 331 Alternate PF14200 Ricin-type beta-trefoil 34 107start lectin domain-like PF05431 Insecticidal Crystal 227 386 Toxin, P42APG06697.2 332 Signal PF14200 Ricin-type beta-trefoil 71 133 peptidelectin domain-like removed PF05431 Insecticidal Crystal 199 358 Toxin,P42 APG06705.0 333 PF03945 delta endotoxin, N- 100 314 terminal domainPF03944 delta endotoxin 518 648 APG06705.1 334 Alternate PF03945 deltaendotoxin, N- 84 298 start terminal domain PF03944 delta endotoxin 502632 APG06705.2 335 Alternate PF03945 delta endotoxin, N- 84 298 startand 3′ terminal domain Truncation PF03944 delta endotoxin 502 631APG06718.0 336 PF01338 Bacillus thuringiensis 33 254 toxin APG06738.0337 PF03318 Clostridium epsilon 105 293 toxin ETX/Bacillus mosquitocidaltoxin MTX2 APG06738.1 338 Alternate PF03318 Clostridium epsilon 64 252start toxin ETX/Bacillus mosquitocidal toxin MTX2 APG06774.0 339 PF03945delta endotoxin, N- 125 311 terminal domain APG06791.0 340 PF03318Clostridium epsilon 138 247 toxin ETX/Bacillus mosquitocidal toxin MTX2APG06791.1 341 Signal PF03318 Clostridium epsilon 104 200 peptide toxinETX/Bacillus removed mosquitocidal toxin MTX2 APG06883.0 342 PF03945delta endotoxin, N- 118 282 terminal domain PF01473 Putative cell wall505 517 binding repeat PF01473 Putative cell wall 538 553 binding repeatAPG06883.1 343 Signal PF03945 delta endotoxin, N- 80 244 peptideterminal domain removed PF01473 Putative cell wall 467 479 bindingrepeat PF01473 Putative cell wall 500 515 binding repeat APG06898.0 344PF14200 Ricin-type beta-trefoil 47 100 lectin domain-like PF05431Insecticidal Crystal 215 379 Toxin, P42 APG06898.1 345 Alternate PF14200Ricin-type beta-trefoil 47 100 start lectin domain-like PF05431Insecticidal Crystal 215 379 Toxin, P42 APG06937.0 346 PF01338 Bacillusthuringiensis 25 234 toxin APG06937.1 347 Alternate PF01338 Bacillusthuringiensis 19 228 start toxin APG06954.0 348 PF03318 Clostridiumepsilon 106 281 toxin ETX/Bacillus mosquitocidal toxin MTX2 APG06954.1349 Alternate PF03318 Clostridium epsilon 102 277 start toxinETX/Bacillus mosquitocidal toxin MTX2 APG07054.0 350 PF12495 Vegetativeinsecticide 11 189 protein 3A N terminal APG07057.0 351 PF03945 deltaendotoxin, N- 4 167 terminal domain PF14200 Ricin-type beta-trefoil 404493 lectin domain-like APG07094.0 352 PF03318 Clostridium epsilon 165332 toxin ETX/Bacillus mosquitocidal toxin MTX2 APG07094.1 353 SignalPF03318 Clostridium epsilon 134 301 peptide toxin ETX/Bacillus removedmosquitocidal toxin MTX2 APG07107.0 354 PF03318 Clostridium epsilon 98314 toxin ETX/Bacillus mosquitocidal toxin MTX2 APG07107.1 355 SignalPF03318 Clostridium epsilon 65 281 peptide toxin ETX/Bacillus removedmosquitocidal toxin MTX2 APG07137.0 356 PF03318 Clostridium epsilon 88287 toxin ETX/Bacillus mosquitocidal toxin MTX2 APG07137.1 357 AlternatePF03318 Clostridium epsilon 72 271 start toxin ETX/Bacillusmosquitocidal toxin MTX2 APG07137.2 358 Signal PF03318 Clostridiumepsilon 45 244 peptide toxin ETX/Bacillus removed mosquitocidal toxinMTX2 APG07162.0 359 PF03945 delta endotoxin, N- 82 272 terminal domainPF03944 delta endotoxin 518 649 APG07176.0 360 PF03318 Clostridiumepsilon 57 268 toxin ETX/Bacillus mosquitocidal toxin MTX2 APG07249.0361 PF03318 Clostridium epsilon 133 227 toxin ETX/Bacillus mosquitocidaltoxin MTX2 APG07249.1 362 Alternate PF03318 Clostridium epsilon 121 215start toxin ETX/Bacillus mosquitocidal toxin MTX2 APG07249.2 363 SignalPF03318 Clostridium epsilon 92 186 peptide toxin ETX/Bacillus removedmosquitocidal toxin MTX2 APG07257.0 364 PF05431 Insecticidal Crystal 276439 Toxin, P42 APG07257 365 no PFAM CryBP1 domains (APG02145.0)APG07307.0 366 no PFAM domains APG07307.1 367 Alternate no PFAM startdomains APG07310.0 368 PF05431 Insecticidal Crystal 301 463 Toxin, P42APG07318.0 369 PF07691 PA14 domain 67 164 PF03495 Clostridial binarytoxin 240 315 B/anthrax toxin PA Ca- binding domain PF17475 Clostridialbinary toxin 318 492 B/anthrax toxin PA domain 2 PF17476 Clostridialbinary toxin 539 639 B/anthrax toxin PA domain 3 APG07318.1 370Alternate PF07691 PA14 domain 64 161 start PF03495 Clostridial binarytoxin 237 312 B/anthrax toxin PA Ca- binding domain PF17475 Clostridialbinary toxin 315 489 B/anthrax toxin PA domain 2 PF17476 Clostridialbinary toxin 536 636 B/anthrax toxin PA domain 3 APG07322.0 371 PF03945delta endotoxin, N- 100 301 terminal domain PF00555 delta endotoxin 309504 PF03944 delta endotoxin 516 644 APG07322.1 372 Alternate PF03945delta endotoxin, N- 97 298 start terminal domain PF00555 delta endotoxin306 501 PF03944 delta endotoxin 513 641 APG07322.2 373 Alternate PF03945delta endotoxin, N- 97 298 start and 3′ terminal domain TruncationPF00555 delta endotoxin 306 501 PF03944 delta endotoxin 513 640 APG07322374 no PFAM SplitCry domains (APG07435.0) APG07432.0 375 PF05431Insecticidal Crystal 287 449 Toxin, P42 APG07432.1 376 Alternate PF05431Insecticidal Crystal 284 446 start Toxin, P42 APG07463.0 377 PF03318Clostridium epsilon 35 163 toxin ETX/Bacillus mosquitocidal toxin MTX2APG07467.0 378 no PFAM domains APG07467.1 379 Signal no PFAM peptidedomains removed APG07487.0 380 PF03318 Clostridium epsilon 85 316 toxinETX/Bacillus mosquitocidal toxin MTX2 APG07511.0 381 PF14200 Ricin-typebeta-trefoil 38 97 lectin domain-like PF14200 Ricin-type beta-trefoil118 179 lectin domain-like PF05431 Insecticidal Crystal 208 368 Toxin,P42 APG07511.1 382 Alternate PF14200 Ricin-type beta-trefoil 37 96 startlectin domain-like PF14200 Ricin-type beta-trefoil 117 178 lectindomain-like PF05431 Insecticidal Crystal 207 367 Toxin, P42 APG07564.0383 PF03945 delta endotoxin, N- 80 294 terminal domain PF03944 deltaendotoxin 495 630 APG07564.1 384 3′ Truncation PF03945 delta endotoxin,N- 80 294 terminal domain PF03944 delta endotoxin 495 630 APG07609.0 385no PFAM domains APG07609.1 386 Signal no PFAM peptide domains removedAPG07636.0 387 PF03945 delta endotoxin, N- 230 327 terminal domainPF03944 delta endotoxin 550 688 APG07636.1 388 3′ Truncation PF03945delta endotoxin, N- 230 327 terminal domain PF03944 delta endotoxin 550687 APG07664.0 389 no PFAM domains APG07664.1 390 Alternate no PFAMstart domains APG07665.0 391 PF03945 delta endotoxin, N- 85 284 terminaldomain PF00555 delta endotoxin 293 502 PF03944 delta endotoxin 512 654APG07665.1 392 Alternate PF03945 delta endotoxin, N- 79 278 start and 3′terminal domain Truncation PF00555 delta endotoxin 287 496 PF03944 deltaendotoxin 506 648 APG07732.0 393 PF03318 Clostridium epsilon 55 263toxin ETX/Bacillus mosquitocidal toxin MTX2 APG07753.0 394 PF03318Clostridium epsilon 117 306 toxin ETX/Bacillus mosquitocidal toxin MTX2APG07753.1 395 Signal PF03318 Clostridium epsilon 85 275 peptide toxinETX/Bacillus removed mosquitocidal toxin MTX2 APG07798.0 396 PF03945delta endotoxin, N- 63 299 terminal domain PF09259 Fungal 999 1085immunomodulatory protein Fve APG07798.1 397 3′ Truncation PF03945 deltaendotoxin, N- 63 299 terminal domain APG07810.0 398 PF03945 deltaendotoxin, N- 89 294 terminal domain PF00555 delta endotoxin 306 480PF03944 delta endotoxin 501 663 APG07811.0 399 PF03945 delta endotoxin,N- 140 316 terminal domain PF14200 Ricin-type beta-trefoil 464 549lectin domain-like APG07811.1 400 Alternate PF03945 delta endotoxin, N-117 299 start terminal domain PF14200 Ricin-type beta-trefoil 441 526lectin domain-like APG07811.2 401 Signal PF03945 delta endotoxin, N- 79255 peptide terminal domain removed PF14200 Ricin-type beta-trefoil 403488 lectin domain-like APG07945.0 402 PF03945 delta endotoxin, N- 115297 terminal domain PF00555 delta endotoxin 305 524 PF03944 deltaendotoxin 534 671 APG07945.1 403 3′ Truncation PF03945 delta endotoxin,N- 115 297 terminal domain PF00555 delta endotoxin 305 524 PF03944 deltaendotoxin 534 671 APG07945 404 PF07029 CryBP1 protein 46 152 CryBP1(APG04357.0) APG07969.0 405 PF03318 Clostridium epsilon 94 244 toxinETX/Bacillus mosquitocidal toxin MTX2 APG07969.1 406 Signal PF03318Clostridium epsilon 72 223 peptide toxin ETX/Bacillus removedmosquitocidal toxin MTX2 APG08004.0 407 PF14200 Ricin-type beta-trefoil32 102 lectin domain-like PF14200 Ricin-type beta-trefoil 111 148 lectindomain-like PF05431 Insecticidal Crystal 231 391 Toxin, P42 APG08004.1408 Signal PF14200 Ricin-type beta-trefoil 5 75 peptide lectindomain-like removed PF14200 Ricin-type beta-trefoil 84 121 lectindomain-like PF05431 Insecticidal Crystal 204 364 Toxin, P42 APG08021.0409 PF03945 delta endotoxin, N- 99 277 terminal domain PF00555 deltaendotoxin 285 476 PF03944 delta endotoxin 487 623 APG08021.1 410 3′Truncation PF03945 delta endotoxin, N- 99 277 terminal domain PF00555delta endotoxin 285 476 PF03944 delta endotoxin 487 622 APG08081.0 411PF00388 Phosphatidylinositol- 94 208 specific phospholipase C, X domainPF14200 Ricin-type beta-trefoil 553 619 lectin domain-like APG08081.1412 Signal PF00388 Phosphatidylinositol- 58 172 peptide specificphospholipase removed C, X domain PF14200 Ricin-type beta-trefoil 517583 lectin domain-like APG08081.2 413 Signal PF00388Phosphatidylinositol- 57 172 peptide specific phospholipase removed andC, X domain 3′ Truncation APG08171.0 414 PF03945 delta endotoxin, N- 60239 terminal domain PF01473 Putative cell wall 316 327 binding repeatAPG08193.0 415 PF14200 Ricin-type beta-trefoil 70 139 lectin domain-likePF05431 Insecticidal Crystal 213 376 Toxin, P42 APG08193.1 416 SignalPF14200 Ricin-type beta-trefoil 43 112 peptide lectin domain-likeremoved PF05431 Insecticidal Crystal 186 349 Toxin, P42 APG08207.0 417PF03318 Clostridium epsilon 241 357 toxin ETX/Bacillus mosquitocidaltoxin MTX2 APG08207.1 418 Alternate PF03318 Clostridium epsilon 219 326start toxin ETX/Bacillus mosquitocidal toxin MTX2 APG08207.2 419 SignalPF03318 Clostridium epsilon 191 301 peptide toxin ETX/Bacillus removedmosquitocidal toxin MTX2 APG08240.0 420 PF03318 Clostridium epsilon 101322 toxin ETX/Bacillus mosquitocidal toxin MTX2 APG08316.0 421 PF03318Clostridium epsilon 5 62 toxin ETX/Bacillus mosquitocidal toxin MTX2APG08335.0 422 PF03318 Clostridium epsilon 107 307 toxin ETX/Bacillusmosquitocidal toxin MTX2 APG08335.1 423 Signal PF03318 Clostridiumepsilon 82 282 peptide toxin ETX/Bacillus removed mosquitocidal toxinMTX2 APG08339.0 424 PF03318 Clostridium epsilon 79 292 toxinETX/Bacillus mosquitocidal toxin MTX2 APG08339.1 425 Signal PF03318Clostridium epsilon 50 263 peptide toxin ETX/Bacillus removedmosquitocidal toxin MTX2 APG08370.0 426 PF01823 MAC/Perforin domain 144342 PF01453 D-mannose binding 388 463 lectin APG08370.1 427 SignalPF01823 MAC/Perforin domain 118 316 peptide PF01453 D-mannose binding362 437 removed lectin APG08378.0 428 PF03945 delta endotoxin, N- 100301 terminal domain PF00555 delta endotoxin 309 504 PF03944 deltaendotoxin 516 644 APG08378.1 429 Alternate PF03945 delta endotoxin, N-97 298 start terminal domain PF00555 delta endotoxin 306 501 PF03944delta endotoxin 513 641 APG08378 430 no PFAM SplitCry domains(APG08000.0) APG08427.0 431 PF06101 Vacuolar protein 22 194sorting-associated protein 62 APG08427.1 432 Alternate PF06101 Vacuolarprotein 14 186 start sorting-associated protein 62 APG08468.0 433PF03945 delta endotoxin, N- 60 238 terminal domain PF01473 Putative cellwall 316 327 binding repeat APG08495.0 434 PF03318 Clostridium epsilon60 271 toxin ETX/Bacillus mosquitocidal toxin MTX2 APG08526.0 435PF03945 delta endotoxin, N- 77 263 terminal domain APG08576.0 436 noPFAM domains APG08577.0 437 PF03318 Clostridium epsilon 41 253 toxinETX/Bacillus mosquitocidal toxin MTX2 APG08624.0 438 PF06101 Vacuolarprotein 18 205 sorting-associated protein 62 APG08624.1 439 AlternatePF06101 Vacuolar protein 16 203 start sorting-associated protein 62APG08625.0 440 PF03318 Clostridium epsilon 84 296 toxin ETX/Bacillusmosquitocidal toxin MTX2 APG08625.1 441 Signal PF03318 Clostridiumepsilon 56 268 peptide toxin ETX/Bacillus removed mosquitocidal toxinMTX2 APG08639.0 442 PF03318 Clostridium epsilon 55 248 toxinETX/Bacillus mosquitocidal toxin MTX2 APG08642.0 443 PF03945 deltaendotoxin, N- 101 308 terminal domain PF00555 delta endotoxin 321 505PF03944 delta endotoxin 529 687 APG08642.1 444 Alternate PF03945 deltaendotoxin, N- 94 301 start terminal domain PF00555 delta endotoxin 314498 PF03944 delta endotoxin 522 680 APG08642.2 445 Alternate PF03945delta endotoxin, N- 94 301 start and 3′ terminal domain TruncationPF00555 delta endotoxin 314 498 PF03944 delta endotoxin 522 680APG08757.0 446 PF03318 Clostridium epsilon 134 333 toxin ETX/Bacillusmosquitocidal toxin MTX2 APG08757.1 447 Signal PF03318 Clostridiumepsilon 107 306 peptide toxin ETX/Bacillus removed mosquitocidal toxinMTX2 APG08765.0 448 PF03945 delta endotoxin, N- 127 301 terminal domainPF00555 delta endotoxin 310 506 PF03944 delta endotoxin 516 670 APG08765449 no PFAM CryBP1 domains (APG07717.0) APG08791.0 450 no PFAM domainsAPG08791.1 451 Alternate no PFAM start domains APG08791.2 452 Signal noPFAM peptide domains removed APG08812.0 453 no PFAM domains APG08823.0454 PF03318 Clostridium epsilon 71 267 toxin ETX/Bacillus mosquitocidaltoxin MTX2 APG08852.0 455 PF03945 delta endotoxin, N- 59 238 terminaldomain APG08912.0 456 PF03318 Clostridium epsilon 96 315 toxinETX/Bacillus mosquitocidal toxin MTX2 APG08912.1 457 Alternate PF03318Clostridium epsilon 93 312 start toxin ETX/Bacillus mosquitocidal toxinMTX2 APG08912.2 458 Signal PF03318 Clostridium epsilon 65 284 peptidetoxin ETX/Bacillus removed mosquitocidal toxin MTX2 APG09027.0 459PF14200 Ricin-type beta-trefoil 32 101 lectin domain-like PF05431Insecticidal Crystal 236 396 Toxin, P42 APG09027.1 460 Signal PF14200Ricin-type beta-trefoil 4 72 peptide lectin domain-like removed PF05431Insecticidal Crystal 207 367 Toxin, P42 APG09028.0 461 PF03945 deltaendotoxin, N- 114 329 terminal domain PF01473 Putative cell wall 341 353binding repeat PF01473 Putative cell wall 360 371 binding repeat PF01473Putative cell wall 387 398 binding repeat PF01473 Putative cell wall 471484 binding repeat PF01473 Putative cell wall 522 536 binding repeatPF02839 Carbohydrate binding 636 661 domain APG09028.1 462 SignalPF03945 delta endotoxin, N- 76 291 peptide terminal domain removedPF01473 Putative cell wall 303 315 binding repeat PF01473 Putative cellwall 322 333 binding repeat PF01473 Putative cell wall 349 360 bindingrepeat PF01473 Putative cell wall 433 446 binding repeat PF01473Putative cell wall 484 498 binding repeat PF02839 Carbohydrate binding598 623 domain APG09048.0 463 PF03318 Clostridium epsilon 73 276 toxinETX/Bacillus mosquitocidal toxin MTX2 APG09048.1 464 Signal PF03318Clostridium epsilon 55 258 peptide toxin ETX/Bacillus removedmosquitocidal toxin MTX2 APG09152.0 465 PF03318 Clostridium epsilon 54250 toxin ETX/Bacillus mosquitocidal toxin MTX2 APG09152.1 466 AlternatePF03318 Clostridium epsilon 49 245 start toxin ETX/Bacillusmosquitocidal toxin MTX2 APG09158.0 467 no PFAM domains APG09158.1 468Signal no PFAM peptide domains removed APG09181.0 469 PF01338 Bacillusthuringiensis 22 243 toxin APG09201.0 470 PF01338 Bacillus thuringiensis17 255 toxin APG09210.0 471 PF03945 delta endotoxin, N- 91 274 terminaldomain APG09221.0 472 PF12495 Vegetative insecticide 10 185 protein 3A Nterminal APG09238.0 473 PF07691 PA14 domain 52 178 PF03495 Clostridialbinary toxin 220 295 B/anthrax toxin PA Ca- binding domain PF17475Clostridial binary toxin 300 492 B/anthrax toxin PA domain 2 PF17476Clostridial binary toxin 528 614 B/anthrax toxin PA domain 3 APG09238.1474 Alternate PF07691 PA14 domain 50 176 start PF03495 Clostridialbinary toxin 218 293 B/anthrax toxin PA Ca- binding domain PF17475Clostridial binary toxin 298 490 B/anthrax toxin PA domain 2 PF17476Clostridial binary toxin 526 612 B/anthrax toxin PA domain 3 APG09238.2475 Signal PF07691 PA14 domain 26 152 peptide PF03495 Clostridial binarytoxin 194 269 removed B/anthrax toxin PA Ca- binding domain PF17475Clostridial binary toxin 274 466 B/anthrax toxin PA domain 2 PF17476Clostridial binary toxin 502 588 B/anthrax toxin PA domain 3 APG09266.0476 PF14200 Ricin-type beta-trefoil 42 102 lactin domain-like PF05431Insecticidal Crystal 214 374 Toxin, P42 APG09266.1 477 Alternate PF14200Ricin-type beta-trefoil 41 101 start lactin domain-like PF05431Insecticidal Crystal 213 373 Toxin, P42 APG09289.0 478 PF03945 deltaendotoxin, N- 85 304 terminal domain PF03944 delta endotoxin 527 647APG09289.1 479 Alternate PF03945 delta endotoxin, N- 81 300 startterminal domain PF03944 delta endotoxin 523 643 APG09289.2 480 AlternatePF03945 delta endotoxin, N- 81 300 start and 3′ terminal domainTruncation PF03944 delta endotoxin 523 642 APG09323.0 481 PF14200Ricin-type beta-trefoil 41 116 lactin domain-like PF05431 InsecticidalCrystal 247 407 Toxin, P42 APG09323.1 482 Alternate PF14200 Ricin-typebeta-trefoil 32 107 start lectin domain-like PF05431 InsecticidalCrystal 238 398 Toxin, P42 APG09412.0 483 PF03318 Clostridium epsilon 92295 toxin ETX/Bacillus mosquitocidal toxin MTX2 APG09412.1 484 SignalPF03318 Clostridium epsilon 60 263 peptide toxin ETX/Bacillus removedmosquitocidal toxin MTX2 APG09415.0 485 no PFAM domains APG09415.1 486Signal no PFAM peptide domains removed APG09416.0 487 PF03318Clostridium epsilon 47 250 toxin ETX/Bacillus mosquitocidal toxin MTX2APG09430.0 488 PF03945 delta endotoxin, N- 109 299 terminal domainPF00555 delta endotoxin 311 483 PF03944 delta endotoxin 494 660APG09430.1 489 3′ Truncation PF03945 delta endotoxin, N- 109 299terminal domain PF00555 delta endotoxin 311 483 PF03944 delta endotoxin494 659 APG09439.0 490 no PFAM domains APG09465.0 491 PF14200 Ricin-typebeta-trefoil 72 151 lectin domain-like PF05431 Insecticidal Crystal 231390 Toxin, P42 APG09465.1 492 Signal PF14200 Ricin-type beta-trefoil 45124 peptide lectin domain-like removed PF05431 Insecticidal Crystal 204363 Toxin, P42 APG09511.0 493 PF03945 delta endotoxin, N- 144 311terminal domain PF14200 Ricin-type beta-trefoil 553 595 lectindomain-like APG09511.1 494 Signal PF03945 delta endotoxin, N- 108 276peptide terminal domain removed PF14200 Ricin-type beta-trefoil 517 559lectin domain-like APG09548.0 495 PF03945 delta endotoxin, N- 86 265terminal domain PF00555 delta endotoxin 358 567 PF03944 delta endotoxin579 711 APG09548.1 496 3′ Truncation PF03945 delta endotoxin, N- 86 265terminal domain PF00555 delta endotoxin 358 567 PF03944 delta endotoxin579 710 APG09548 497 no PFAM SplitCry domains (APG01177.0) APG09675.0498 PF03318 Clostridium epsilon 58 279 toxin ETX/Bacillus mosquitocidaltoxin MTX2 APG09773.0 499 PF03945 delta endotoxin, N- 54 127 terminaldomain PF03945 delta endotoxin, N- 218 293 terminal domain APG09812.0500 PF03945 delta endotoxin, N- 21 172 terminal domain APG09814.0 501PF03945 delta endotoxin, N- 86 290 terminal domain PF00555 deltaendotoxin 298 517 PF03944 delta endotoxin 527 683 APG09826.0 502 PF03945delta endotoxin, N- 74 258 terminal domain APG09826.1 503 AlternatePF03945 delta endotoxin, N- 28 212 start terminal domain APG09835.0 504PF03945 delta endotoxin, N- 60 239 terminal domain PF01473 Putative cellwall 316 327 binding repeat APG09895.0 505 PF03945 delta endotoxin, N-128 311 terminal domain PF14200 Ricin-type beta-trefoil 519 605 lectindomain-like APG09895.1 506 Signal PF03945 delta endotoxin, N- 94 277peptide terminal domain removed PF14200 Ricin-type beta-trefoil 485 571lectin domain-like APG09901.0 507 PF03318 Clostridium epsilon 96 273toxin ETX/Bacillus mosquitocidal toxin MTX2 APG09901.1 508 AlternatePF03318 Clostridium epsilon 93 270 start toxin ETX/Bacillusmosquitocidal toxin MTX2 APG09901.2 509 Signal PF03318 Clostridiumepsilon 65 242 peptide toxin ETX/Bacillus removed mosquitocidal toxinMTX2 APG09903.0 510 PF03945 delta endotoxin, N- 65 234 terminal domainPF00555 delta endotoxin 375 559 PF03944 delta endotoxin 607 735APG09903.1 511 Alternate PF03945 delta endotoxin, N- 65 234 startterminal domain PF00555 delta endotoxin 375 559 PF03944 delta endotoxin607 735 APG10001.0 512 PF03318 Clostridium epsilon 112 317 toxinETX/Bacillus mosquitocidal toxin MTX2 APG10001.1 513 Alternate PF03318Clostridium epsilon 92 297 start toxin ETX/Bacillus mosquitocidal toxinMTX2 APG10001.2 514 Signal PF03318 Clostridium epsilon 58 263 peptidetoxin ETX/Bacillus removed mosquitocidal toxin MTX2 APG01902.0 515PF03945 delta endotoxin, N- 21 172 terminal domain APG01902.1 516Alternate PF03945 delta endotoxin, N- 21 172 start terminal domainAPG02996.0 517 PF03945 delta endotoxin, N- 22 172 terminal domainAPG02996.1 518 Alternate PF03945 delta endotoxin, N- 22 172 startterminal domain APG03098.0 519 PF03945 delta endotoxin, N- 21 172terminal domain APG03098.1 520 Alternate PF03945 delta endotoxin, N- 21172 start terminal domain APG04517.0 521 PF03945 delta endotoxin, N- 20174 terminal domain APG04619.0 522 PF03945 delta endotoxin, N- 21 172terminal domain APG04619.1 523 Alternate PF03945 delta endotoxin, N- 21172 start terminal domain APG06717.0 524 PF03945 delta endotoxin, N- 21172 terminal domain APG06717.1 525 Alternate PF03945 delta endotoxin, N-21 172 start terminal domain

Recombinant or synthetic nucleic acids encoding the pesticidalpolypeptides disclosed herein are also provided. Of particular interestare nucleic acid sequences that have been designed for expression in aplant of interest. That is, the nucleic acid sequence can be optimizedfor increased expression in a host plant. A pesticidal protein of theinvention can be back-translated to produce a nucleic acid comprisingcodons optimized for expression in a particular host, for example, acrop plant. In another embodiment, the polynucleotides encoding thepolypeptides provided herein may be optimized for increased expressionin the transformed plant. That is, the polynucleotides can besynthesized using plant-preferred codons for improved expression. See,for example, Campbell and Gowri (1990) Plant Physiol. 92:1-11 for adiscussion of host-preferred codon usage. Methods are available in theart for synthesizing plant-preferred genes. See, for example, U.S. Pat.Nos. 5,380,831, and 5,436,391, and Murray et al. (1989) Nucleic AcidsRes. 17:477-498, herein incorporated by reference. Expression of such acoding sequence by the transformed plant (e.g., dicot or monocot) willresult in the production of a pesticidal polypeptide and conferincreased resistance in the plant to a pest. Recombinant and syntheticnucleic acid molecules encoding the pesticidal proteins of the inventiondo not include the naturally occurring bacterial sequence encoding theprotein.

A “recombinant polynucleotide” or “recombinant nucleic acid” comprises acombination of two or more chemically linked nucleic acid segments whichare not found directly joined in nature. By “directly joined” isintended the two nucleic acid segments are immediately adjacent andjoined to one another by a chemical linkage. In specific embodiments,the recombinant polynucleotide comprises a polynucleotide of interest ora variant or fragment thereof such that an additional chemically linkednucleic acid segment is located either 5′, 3′ or internal to thepolynucleotide of interest. Alternatively, the chemically-linked nucleicacid segment of the recombinant polynucleotide can be formed by deletionof a sequence. The additional chemically linked nucleic acid segment orthe sequence deleted to join the linked nucleic acid segments can be ofany length, including for example, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20or greater nucleotides. Various methods for making such recombinantpolynucleotides include chemical synthesis or by the manipulation ofisolated segments of polynucleotides by genetic engineering techniques.In specific embodiments, the recombinant polynucleotide can comprise arecombinant DNA sequence or a recombinant RNA sequence. A “fragment of arecombinant polynucleotide or nucleic acid” comprises at least one of acombination of two or more chemically linked amino acid segments whichare not found directly joined in nature.

Fragments of a polynucleotide (RNA or DNA) may encode protein fragmentsthat retain activity. In specific embodiments, a fragment of arecombinant polynucleotide or a recombinant polynucleotide constructcomprises at least one junction of the two or more chemically linked oroperably linked nucleic acid segments which are not found directlyjoined in nature, A fragment of a polynucleotide that encodes abiologically active portion of a polypeptide that retains pesticidalactivity will encode at least 25, 30, 40, 50, 60, 70, 75, 80, 90, 100,110, 120, 125, 130, 140, 150, 160, 170, 175, 180, contiguous aminoacids, or up to the total number of amino acids present in a full-lengthpolypeptide as set forth in SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10,11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28,29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46,47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64,65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82,83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100,101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114,115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128,129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142,143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156,157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170,171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184,185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198,199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212,213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226,227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240,241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254,255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268,269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282,283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296,297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310,311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324,325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338,339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352,353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366,367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380,381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394,395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406, 407, 408,409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421, 422,423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435, 436,437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449, 450,451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 461, 462, 463, 464,465, 466, 467, 468, 469, 470, 471, 472, 473, 474, 475, 476, 477, 478,479, 480, 481, 482, 483, 484, 485, 486, 487, 488, 489, 490, 491, 492,493, 494, 495, 496, 497, 498, 499, 500, 501, 502, 503, 504, 505, 506,507, 508, 509, 510, 511, 512, 513, 514, 515, 516, 517, 518, 519, 520,521, 522, 523, 524, and/or 525. In specific embodiments, suchpolypeptide fragments are active fragment, and in still otherembodiments, the polypeptide fragment comprises a recombinantpolypeptide fragment. As used herein, a fragment of a recombinantpolypeptide comprises at least one of a combination of two or morechemically linked amino acid segments which are not found directlyjoined in nature.

By “variants” is intended to mean substantially similar sequences. Forpolynucleotides, a variant comprises a deletion and/or addition of oneor more nucleotides at one or more internal sites within the nativepolynucleotide and/or a substitution of one or more nucleotides at oneor more sites in the native polynucleotide. As used herein, a “native”polynucleotide or polypeptide comprises a naturally occurring nucleotidesequence or amino acid sequence, respectively.

Variants of a particular polynucleotide of the invention (i.e., thereference polynucleotide) can also be evaluated by comparison of thepercent sequence identity between the polypeptide encoded by a variantpolynucleotide and the polypeptide encoded by the referencepolynucleotide. Thus, for example, an isolated polynucleotide thatencodes a polypeptide with a given percent sequence identity to thepolypeptide of SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14,15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32,33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50,51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68,69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86,87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103,104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117,118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131,132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145,146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159,160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173,174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187,188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201,202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215,216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229,230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243,244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257,258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271,272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285,286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299,300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313,314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327,328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341,342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355,356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369,370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383,384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397,398, 399, 400, 401, 402, 403, 404, 405, 406, 407, 408, 409, 410, 411,412, 413, 414, 415, 416, 417, 418, 419, 420, 421, 422, 423, 424, 425,426, 427, 428, 429, 430, 431, 432, 433, 434, 435, 436, 437, 438, 439,440, 441, 442, 443, 444, 445, 446, 447, 448, 449, 450, 451, 452, 453,454, 455, 456, 457, 458, 459, 460, 461, 462, 463, 464, 465, 466, 467,468, 469, 470, 471, 472, 473, 474, 475, 476, 477, 478, 479, 480, 481,482, 483, 484, 485, 486, 487, 488, 489, 490, 491, 492, 493, 494, 495,496, 497, 498, 499, 500, 501, 502, 503, 504, 505, 506, 507, 508, 509,510, 511, 512, 513, 514, 515, 516, 517, 518, 519, 520, 521, 522, 523,524, and/or 525 are disclosed. Percent sequence identity between any twopolypeptides can be calculated using sequence alignment programs andparameters described elsewhere herein. Where any given pair ofpolynucleotides of the invention is evaluated by comparison of thepercent sequence identity shared by the two polypeptides they encode,the percent sequence identity between the two encoded polypeptides is atleast about 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 86%, 87%,88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or moresequence identity to SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12,13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30,31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48,49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66,67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84,85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101,102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115,116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129,130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143,144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157,158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171,172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185,186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199,200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213,214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227,228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241,242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255,256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269,270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283,284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297,298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311,312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325,326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339,340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353,354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367,368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381,382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395,396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406, 407, 408, 409,410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421, 422, 423,424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435, 436, 437,438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449, 450, 451,452, 453, 454, 455, 456, 457, 458, 459, 460, 461, 462, 463, 464, 465,466, 467, 468, 469, 470, 471, 472, 473, 474, 475, 476, 477, 478, 479,480, 481, 482, 483, 484, 485, 486, 487, 488, 489, 490, 491, 492, 493,494, 495, 496, 497, 498, 499, 500, 501, 502, 503, 504, 505, 506, 507,508, 509, 510, 511, 512, 513, 514, 515, 516, 517, 518, 519, 520, 521,522, 523, 524, and/or 525. In other embodiments, the variant of thepolynucleotide provided herein differs from the native sequence by atleast 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or more nucleotides.

Variant polynucleotide and proteins also encompass sequences andproteins derived from a mutagenic and recombinogenic procedure such asDNA shuffling. With such a procedure, one or more different pesticidalprotein disclosed herein (SEQ ID NO: 1-525) is manipulated to create anew pesticidal protein possessing the desired properties. In thismanner, libraries of recombinant polynucleotides are generated from apopulation of related sequence polynucleotides comprising sequenceregions that have substantial sequence identity and can be homologouslyrecombined in vitro or in vivo. For example, using this approach,sequence motifs encoding a domain of interest may be shuffled betweenthe pesticidal sequences provided herein and other known pesticidalgenes to obtain a new gene coding for a protein with an improvedproperty of interest, such as an increased K_(m) in the case of anenzyme. Strategies for such DNA shuffling are known in the art. See, forexample, Stemmer (1994) Proc. Natl. Acad. Sci. USA 91:10747-10751;Stemmer (1994) Nature 370:389-391; Crameri et al. (1997) Nature Biotech.15:436-438; Moore et al. (1997) J. Mol. Biol. 272:336-347; Zhang et al.(1997) Proc. Natl. Acad. Sci. USA 94:4504-4509; Crameri et al. (1998)Nature 391:288-291; and U.S. Pat. Nos. 5,605,793 and 5,837,458. A“shuffled” nucleic acid is a nucleic acid produced by a shufflingprocedure such as any shuffling procedure set forth herein. Shufflednucleic acids are produced by recombining (physically or virtually) twoor more nucleic acids (or character strings), for example in anartificial, and optionally recursive, fashion. Generally, one or morescreening steps are used in shuffling processes to identify nucleicacids of interest; this screening step can be performed before or afterany recombination step. In some (but not all) shuffling embodiments, itis desirable to perform multiple rounds of recombination prior toselection to increase the diversity of the pool to be screened. Theoverall process of recombination and selection are optionally repeatedrecursively. Depending on context, shuffling can refer to an overallprocess of recombination and selection, or, alternately, can simplyrefer to the recombinational portions of the overall process.

In one embodiment, a method of obtaining a polynucleotide that encodesan improved polypeptide comprising pesticidal activity is provided,wherein the improved polypeptide has at least one improved property overany one of SEQ ID NOs: 1-525. Such methods can comprises (a) recombininga plurality of parental polynucleotides to produce a library ofrecombinant polynucleotides encoding recombinant pesticidalpolypeptides; (b) screening the library to identify a recombinantpolynucleotide that encodes an improved recombinant pesticidalpolypeptide that has an enhanced property improved over the parentalpolynucleotide; (c) recovering the recombinant polynucleotide thatencodes the improved recombinant pesticidal polypeptide identified in(b); and, (d) repeating steps (a), (b) and (c) using the recombinantpolynucleotide recovered in step (c) as one of the plurality of parentalpolynucleotides in repeated step (a).

iii. Sequence Comparisons

As used herein, the term “identity” or “percent identity” when used withrespect to a particular pair of aligned amino acid sequences, refers tothe percent amino acid sequence identity that is obtained by countingthe number of identical matches in the alignment and dividing suchnumber of identical matches by the length of the aligned sequences. Asused herein, the term “similarity” or “percent similarity” when usedwith respect to a particular pair of aligned amino acid sequences,refers to the sum of the scores that are obtained from a scoring matrixfor each amino acid pair in the alignment divided by the length of thealigned sequences.

Unless otherwise stated, identity and similarity will be calculated bythe Needleman-Wunsch global alignment and scoring algorithms (Needlemanand Wunsch (1970) J. Mol. Biol. 48(3):443-453) as implemented by the“needle” program, distributed as part of the EMBOSS software package(Rice, P. Longden, I. and Bleasby, A., EMBOSS: The European MolecularBiology Open Software Suite, 2000, Trends in Genetics 16, (6) pp276-277, versions 6.3.1 available from EMBnet atembnet.org/resource/emboss and emboss.sourceforge.net, among othersources) using default gap penalties and scoring matrices (EBLOSUM62 forprotein and EDNAFULL for DNA). Equivalent programs may also be used. By“equivalent program” is intended any sequence comparison program that,for any two sequences in question, generates an alignment havingidentical nucleotide residue matches and an identical percent sequenceidentity when compared to the corresponding alignment generated byneedle from EMBOSS version 6.3.1.

Additional mathematical algorithms are known in the art and can beutilized for the comparison of two sequences. See, for example, thealgorithm of Karlin and Altschul (1990) Proc. Natl. Acad. Sci. USA87:2264, modified as in Karlin and Altschul (1993) Proc. Natl. Acad.Sci. USA 90:5873-5877. Such an algorithm is incorporated into the BLASTprograms of Altschul et al. (1990) J. Mol. Biol. 215:403. BLASTnucleotide searches can be performed with the BLASTN program (nucleotidequery searched against nucleotide sequences) to obtain nucleotidesequences homologous to pesticidal-like nucleic acid molecules of theinvention, or with the BLASTX program (translated nucleotide querysearched against protein sequences) to obtain protein sequenceshomologous to pesticidal nucleic acid molecules of the invention. BLASTprotein searches can be performed with the BLASTP program (protein querysearched against protein sequences) to obtain amino acid sequenceshomologous to pesticidal protein molecules of the invention, or with theTBLASTN program (protein query searched against translated nucleotidesequences) to obtain nucleotide sequences homologous to pesticidalprotein molecules of the invention. To obtain gapped alignments forcomparison purposes, Gapped BLAST (in BLAST 2.0) can be utilized asdescribed in Altschul et al. (1997) Nucleic Acids Res. 25:3389.Alternatively, PSI-Blast can be used to perform an iterated search thatdetects distant relationships between molecules. See Altschul et al.(1997) supra. When utilizing BLAST, Gapped BLAST, and PSI-Blastprograms, the default parameters of the respective programs (e.g.,BLASTX and BLASTN) can be used. Alignment may also be performed manuallyby inspection.

Two sequences are “optimally aligned” when they are aligned forsimilarity scoring using a defined amino acid substitution matrix (e.g.,BLOSUM62), gap existence penalty and gap extension penalty so as toarrive at the highest score possible for that pair of sequences. Aminoacid substitution matrices and their use in quantifying the similaritybetween two sequences are well-known in the art and described, e.g., inDayhoff et al. (1978) “A model of evolutionary change in proteins.” In“Atlas of Protein Sequence and Structure,” Vol. 5, Suppl. 3 (ed. M. O.Dayhoff), pp. 345-352. Natl. Biomed. Res. Found., Washington, D.C. andHenikoff et al. (1992) Proc. Natl. Acad. Sci. USA 89:10915-10919. TheBLOSUM62 matrix is often used as a default scoring substitution matrixin sequence alignment protocols. The gap existence penalty is imposedfor the introduction of a single amino acid gap in one of the alignedsequences, and the gap extension penalty is imposed for each additionalempty amino acid position inserted into an already opened gap. Thealignment is defined by the amino acids positions of each sequence atwhich the alignment begins and ends, and optionally by the insertion ofa gap or multiple gaps in one or both sequences, so as to arrive at thehighest possible score. While optimal alignment and scoring can beaccomplished manually, the process is facilitated by the use of acomputer-implemented alignment algorithm, e.g., gapped BLAST 2.0,described in Altschul et al. (1997) Nucleic Acids Res. 25:3389-3402, andmade available to the public at the National Center for BiotechnologyInformation Website (www.ncbi.nlm.nih.gov). Optimal alignments,including multiple alignments, can be prepared using, e.g., PSI-BLAST,available through www.ncbi.nlm.nih.gov and described by Altschul et al.(1997) Nucleic Acids Res. 25:3389-3402.

With respect to an amino acid sequence that is optimally aligned with areference sequence, an amino acid residue “corresponds to” the positionin the reference sequence with which the residue is paired in thealignment. The “position” is denoted by a number that sequentiallyidentifies each amino acid in the reference sequence based on itsposition relative to the N-terminus. For example, in SEQ ID NO: 1position 1 is V, position 2 is F, position 3 is L, etc. When a testsequence is optimally aligned with SEQ ID NO: 1, a residue in the testsequence that aligns with the L at position 3 is said to “correspond toposition 3” of SEQ ID NO: 1. Owing to deletions, insertion, truncations,fusions, etc., that must be taken into account when determining anoptimal alignment, in general the amino acid residue number in a testsequence as determined by simply counting from the N-terminal will notnecessarily be the same as the number of its corresponding position inthe reference sequence. For example, in a case where there is a deletionin an aligned test sequence, there will be no amino acid thatcorresponds to a position in the reference sequence at the site ofdeletion. Where there is an insertion in an aligned reference sequence,that insertion will not correspond to any amino acid position in thereference sequence. In the case of truncations or fusions there can bestretches of amino acids in either the reference or aligned sequencethat do not correspond to any amino acid in the corresponding sequence.

iv. Antibodies

Antibodies to the polypeptides of the present invention, or to variantsor fragments thereof, are also encompassed. Methods for producingantibodies are well known in the art (see, for example, Harlow and Lane(1988) Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory,Cold Spring Harbor, N.Y.; and U.S. Pat. No. 4,196,265). These antibodiescan be used in kits for the detection and isolation of toxinpolypeptides. Thus, this disclosure provides kits comprising antibodiesthat specifically bind to the polypeptides described herein, including,for example, polypeptides having the sequence of SEQ ID NOs: 1, 2, 3, 4,5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23,24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41,42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59,60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77,78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95,96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110,111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124,125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138,139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152,153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166,167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180,181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194,195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208,209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222,223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236,237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250,251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264,265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278,279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292,293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306,307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320,321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334,335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348,349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362,363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376,377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390,391, 392, 393, 394, 395, 396, 397, 398, 399, 400, 401, 402, 403, 404,405, 406, 407, 408, 409, 410, 411, 412, 413, 414, 415, 416, 417, 418,419, 420, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432,433, 434, 435, 436, 437, 438, 439, 440, 441, 442, 443, 444, 445, 446,447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460,461, 462, 463, 464, 465, 466, 467, 468, 469, 470, 471, 472, 473, 474,475, 476, 477, 478, 479, 480, 481, 482, 483, 484, 485, 486, 487, 488,489, 490, 491, 492, 493, 494, 495, 496, 497, 498, 499, 500, 501, 502,503, 504, 505, 506, 507, 508, 509, 510, 511, 512, 513, 514, 515, 516,517, 518, 519, 520, 521, 522, 523, 524, and/or 525.

II. Pests

The compositions and methods provided herein are useful against avariety of pests. “Pests” includes but is not limited to, insects,fungi, bacteria, nematodes, acarids, protozoan pathogens,animal-parasitic liver flukes, and the like. Pests of particularinterest are insect pests, particularly insect pests that causesignificant damage to agricultural plants. Insect pests include insectsselected from the orders Coleoptera, Diptera, Hymenoptera, Lepidoptera,Mallophaga, Homoptera, Hemiptera, Orthroptera, Thysanoptera, Dermaptera,Isoptera, Anoplura, Siphonaptera, Trichoptera, or nematodes. Innon-limiting embodiments, the insect pest comprises Western cornrootworm, Diabrotica virgifera virgifera; Fall armyworm, Spodopterafrugiperda; Colorado potato beetle, Leptinotarsa decemlineata; Cornearworm, Helicoverpa zea (in North America same species attacks cottonand called cotton bollworm); European corn borer, Ostrinia nubilalis;Black cutworm, Agrotis ipsilon; Diamondback moth, Plutella xylostella;Velvetbean caterpillar, Anticarsia gemmatalis; Southwestern corn borer,Diatraea grandiosella; Cotton bollworm, Helicoverpa armigera (foundother than USA in rest of the world); Southern green stink bug, Nezaraviridula; Green stink bug, Chinavia halaris; Brown marmorated stink bug,Halyomorpha halys; and Brown stink bug, Euschistus servus, Euschistusheros (Neotropical brown stink bug OR soy stink bug); Piezodorusguildinii (red-banded stink bug); Dichelops melacanthus (no common name)and/or Dichelops furcatus (no common name); an aphid, such as a soybeanaphid. In other embodiments, the pest comprises a nematode including,but not limited to, Meloidogyne hapla (Northern root-knot nematode);Meloidogyne enterolobii, Meloidogyne arenaria (peanut root-knotnematode); and Meloidogyne javanica.

The term “insect pests” as used herein refers to insects and othersimilar pests such as, for example, those of the order Acari including,but not limited to, mites and ticks. Insect pests of the presentinvention include, but are not limited to, insects of the orderLepidoptera, e.g. Achoroia grisella, Acleris gloverana, Acleris variana,Adoxophyes orana, Agrotis ipsilon, Alabama argillacea, Alsophilapometaria, Amyelois transitella, Anagasta kuehniella, Anarsialineatella, Anisota senatoria, Antheraea pernyi, Anticarsia gemmatalis,Archips sp., Argyrotaenia sp., Athetis mindara, Bombyx mori, Bucculatrixthurberiella, Cadra cautella, Choristoneura sp., Cochylls hospes, Coliaseurytheme, Corcyra cephalonica, Cydia latiferreanus, Cydia pomonella,Datana integerrima, Dendrolimus sibericus, Desmiafeneralis, Diaphaniahyalinata, Diaphania nitidalis, Diatraea grandiosella, Diatraeasaccharalis, Ennomos subsignaria, Eoreuma loftini, Esphestia elutella,Erannis tilaria, Estigmene acrea, Eulia salubricola, Eupocoelliaambiguella, Eupoecilia ambiguella, Euproctis chrysorrhoea, Euxoamessoria, Galleria mellonella, Grapholita molesta, Harrisina americana,Helicoverpa subflexa, Helicoverpa zea, Heliothis virescens, Hemileucaoliviae, Homoeosoma electellum, Hyphantia cunea, Keiferialycopersicella, Lambdina fiscellaria fiscellaria, Lambdina fiscellarialugubrosa, Leucoma salicis, Lobesia botrana, Loxostege sticticalis,Lymantria dispar, Macalla thyrisalis, Malacosoma sp., Mamestrabrassicae, Mamestra configurata, Manduca quinquemaculata, Manduca sexta,Maruca testulalis, Melanchra picta, Operophtera brumata, Orgyia sp.,Ostrinia nubilalis, Paleacrita vernata, Papilio cresphontes,Pectinophora gossypiella, Phryganidia californica, Phyllonorycterblancardella, Pieris napi, Pieris rapae, Plathypena scabra, Platynotaflouendana, Platynota stultana, Platyptilia carduidactyla, Plodiainterpunctella, Plutella xylostella, Pontia protodice, Pseudaletiaunipuncta, Pseudoplasia includens, Sabulodes aegrotata, Schizuraconcinna, Sitotroga cerealella, Spilonta ocellana, Spodoptera sp.,Thaurnstopoea pityocampa, Tinsola bisselliella, Trichoplusia hi, Udearubigalis, Xylomyges curiails, and Yponomeuta padella.

Insect pests also include insects selected from the orders Diptera,Hymenoptera, Lepidoptera, Mallophaga, Homoptera, Hemiptera, Orthroptera,Thysanoptera, Dermaptera, Isoptera, Anoplura, Siphonaptera, Trichoptera,Coleoptera. Insect pests of the invention for the major crops include,but are not limited to: Maize: Ostrinia nubilalis, European corn borer;Agrotis ipsilon, black cutworm; Helicoverpa zeae, corn earworm;Spodoptera frugiperda, fall armyworm; Diatraea grandiosella,southwestern corn borer; Elasmopalpus lignosellus, lesser cornstalkborer; Diatraea saccharalis, surgarcane borer; western corn rootworm,e.g., Diabrotica virgifera virgifera; northern corn rootworm, e.g.,Diabrotica longicornis barberi; southern corn rootworm, e.g., Diabroticaundecimpunctata howardi; Melanotus spp., wireworms; Cyclocephalaborealis, northern masked chafer (white grub); Cyclocephala immaculata,southern masked chafer (white grub); Popillia japonica, Japanese beetle;Chaetocnema pulicaria, corn flea beetle; Sphenophorus maidis, maizebillbug; Rhopalosiphum maidis, corn leaf aphid; Anuraphis maidiradicis,corn root aphid; Euschistus heros (Neotropical brown stink bug OR soystink bug); Piezodorus guildinii (red-banded stink bug); Dichelopsmelacanthus (no common name); Dichelops furcatus (no common name);Blissus leucopterus leucopterus, chinch bug; Melanoplus femurrubrum,redlegged grasshopper; Melanoplus sanguinipes, migratory grasshopper;Hylemya platura, seedcorn maggot; Agromyza parvicornis, corn blotchleafminer; Anaphothrips obscrurus, grass thrips; Solenopsis milesta,thief ant; Tetranychus urticae, two spotted spider mite; Sorghum: Chilopartellus, sorghum borer; Spodoptera frugiperda, fall armyworm;Helicoverpa zea, corn earworm; Elasmopalpus lignosellus, leser cornstalkborer; Feltia subterranea, granulate cutworm; Phyllophaga crinita, whitegrub; Eleodes, Conoderus, and Aeolus spp., wireworms; Oulema melanopus,cereal leaf beetle; Chaetocnema pulicaria, corn flea beetle;Sphenophorus maidis, maize billbug; Rhopalosiphum maidis; corn leafaphid; Sipha flava, yellow sugarcane aphid; chinch bug, e.g., Blissusleucopterus leucopterus; Contarinia sorghicola, sorghum midge;Tetranychus cinnabarinus, carmine spider mite; Tetranychus urticae,two-spotted spider mite; Wheat: Pseudaletia unipunctata, army worm;Spodoptera frugiperda, fall armyworm; Elasmopalpus lignosellus, lessercornstalk borer; Agrotis orthogonia, pale western cutworm; Elasmopalpuslignosellus, lesser cornstalk borer; Oulema melanopus, cereal leafbeetle; Hypera punctata, clover leaf weevil; southern corn rootworm,e.g., Diabrotica undecimpunctata howardi; Russian wheat aphid;Schizaphis graminum, greenbug; Macrosiphum avenae, English grain aphid;Melanoplus femurrubrum, redlegged grasshopper; Melanoplusdifferentialis, differential grasshopper; Melanoplus sanguinipes,migratory grasshopper; Mayetiola destructor, Hessian fly; Sitodiplosismosellana, wheat midge; Meromyza americana, wheat stem maggot; Hylemyacoarctata, wheat bulb fly; Frankliniella fusca, tobacco thrips; Cephuscinctus, wheat stem sawfly; Aceria tulipae, wheat curl mite; Sunflower:Cylindrocupturus adspersus, sunflower stem weevil; Smicronyx fulus, redsunflower seed weevil; Smicronyx sordidus, gray sunflower seed weevil;Suleima helianthana, sunflower bud moth; Homoeosoma electellum,sunflower moth; Zygogramma exclamationis, sunflower beetle; Bothyrusgibbosus, carrot beetle; Neolasioptera murtfeldtiana, sunflower seedmidge; Cotton: Heliothis virescens, tobacco budworm; Helicoverpa zea,cotton bollworm; Spodoptera exigua, beet armyworm; Pectinophoragossypiella, pink bollworm; boll weevil, e.g., Anthonomus grandis; Aphisgossypii, cotton aphid; Pseudatomoscelis seriatus, cotton fleahopper;Trialeurodes abutilonea, bandedwinged whitefly; Lygus lineolaris,tarnished plant bug; Melanoplus femurrubrum, redlegged grasshopper;Melanoplus differentialis, differential grasshopper; Thrips tabaci,onion thrips; Franklinkiella fusca, tobacco thrips; Tetranychuscinnabarinus, carmine spider mite; Tetranychus urticae, two-spottedspider mite; Rice: Diatraea saccharalis, sugarcane borer; Spodopterafrugiperda, fall armyworm; Helicoverpa zea, corn earworm; Colaspisbrunnea, grape colaspis; Lissorhoptrus oryzophilus, rice water weevil;Sitophilus oryzae, rice weevil; Nephotettix nigropictus, rice leafhoper;chinch bug, e.g., Blissus leucopterus leucopterus; Acrosternum hilare,green stink bug; Soybean: Pseudoplusia includens, soybean looper;Anticarsia gemmatalis, velvetbean caterpillar; Plathypena scabra, greencloverworm; Ostrinia nubilalis, European corn borer; Agrotis ipsilon,black cutworm; Spodoptera exigua, beet armyworm; Heliothis virescens,tobacco budworm; Helicoverpa zea, cotton bollworm; Epilachna varivestis,Mexican bean beetle; Myzus persicae, green peach aphid; Empoasca fabae,potato leafhopper; Acrosternum hilare, green stink bug; Melanoplusfemurrubrum, redlegged grasshopper; Melanoplus differentialis,differential grasshopper; Hylemya platura, seedcorn maggot; Sericothripsvariabilis, soybean thrips; Thrips tabaci, onion thrips; Tetranychusturkestani, strawberry spider mite; Tetranychus urticae, two-spottedspider mite; Barley: Ostrinia nubilalis, European corn borer; Agrotisipsilon, black cutworm; Schizaphis graminum, greenbug; chinch bug, e.g.,Blissus leucopterus leucopterus; Acrosternum hilare, green stink bug;Euschistus serous, brown stink bug; Jylemya platura, seedcorn maggot;Mayetiola destructor, Hessian fly; Petrobia latens, brown wheat mite;Oil Seed Rape: Vrevicoryne brassicae, cabbage aphid; Phyllotretacruciferae, crucifer flea beetle; Phyllotreta striolata, striped fleabeetle; Phyllotreta nemorum, striped turnip flea beetle; Meligethesaeneus, rapeseed beetle; and the pollen beetles Meligethes rufimanus,Meligethes nigrescens, Meligethes canadianus, and Meligethesviridescens; Potato: Leptinotarsa decemlineata, Colorado potato beetle.

The methods and compositions provided herein may be effective againstHemiptera such as Lygus hesperus, Lygus lineolaris, Lygus pratensis,Lygus rugulipennis Popp, Lygus pabulinus, Calocoris norvegicus, Orthopscompestris, Plesiocoris rugicollis, Cyrtopeltis modestus, Cyrtopeltisnotatus, Spanagonicus albofasciatus, Diaphnocoris chlorinonis,Labopidicola allii, Pseudatomoscelis seriatus, Adelphocoris rapidus,Poecilocapsus lineatus, Blissus leucopterus, Nysius ericae, Nysiusraphanus, Euschistus serous, Nezara viridula, Eurygaster, Coreidae,Pyrrhocoridae, Tinidae, Blostomatidae, Reduviidae, and Cimicidae. Pestsof interest also include Araecerus fasciculatus, coffee bean weevil;Acanthoscelides obtectus, bean weevil; Bruchus rufmanus, broadbeanweevil; Bruchus pisorum, pea weevil; Zabrotes subfasciatus, Mexican beanweevil; Diabrotica balteata, banded cucumber beetle; Cerotomatrifurcata, bean leaf beetle; Diabrotica virgifera, Mexican cornrootworm; Epitrix cucumeris, potato flea beetle; Chaetocnema confinis,sweet potato flea beetle; Hypera postica, alfalfa weevil; Anthonomusquadrigibbus, apple curculio; Sternechus paludatus, bean stalk weevil;Hypera brunnipennis, Egyptian alfalfa weevil; Sitophilus granaries,granary weevil; Craponius inaequalis, grape curculio; Sitophiluszeamais, maize weevil; Conotrachelus nenuphar, plum curculio; Euscepespostfaciatus, West Indian sweet potato weevil; Maladera castanea,Asiatic garden beetle; Rhizotrogus majalis, European chafer;Macrodactylus subspinosus, rose chafer; Tribolium confusum, confusedflour beetle; Tenebrio obscurus, dark mealworm; Tribolium castaneum, redflour beetle; Tenebrio molitor, yellow mealworm.

Nematodes include parasitic nematodes such as root-knot, cyst, andlesion nematodes, including Heterodera spp., Meloidogyne spp., andGlobodera spp.; particularly members of the cyst nematodes, including,but not limited to, Heterodera glycines (soybean cyst nematode);Heterodera schachtii (beet cyst nematode); Heterodera avenae (cerealcyst nematode); and Globodera rostochiensis and Globodera pailida(potato cyst nematodes). Lesion nematodes include Pratylenchus spp.

Insect pests may be tested for pesticidal activity of compositions ofthe invention in early developmental stages, e.g., as larvae or otherimmature forms. The insects may be reared in total darkness at fromabout 20° C. to about 30° C. and from about 30% to about 70% relativehumidity. Bioassays may be performed as described in Czapla and Lang(1990) J. Econ. Entomol. 83 (6): 2480-2485. See, also the experimentalsection herein.

III. Expression Cassettes

Polynucleotides encoding the pesticidal proteins provided herein can beprovided in expression cassettes for expression in an organism ofinterest. The cassette will include 5′ and 3′ regulatory sequencesoperably linked to a polynucleotide encoding a pesticidal polypeptideprovided herein that allows for expression of the polynucleotide. Thecassette may additionally contain at least one additional gene orgenetic element to be cotransformed into the organism. Where additionalgenes or elements are included, the components are operably linked.Alternatively, the additional gene(s) or element(s) can be provided onmultiple expression cassettes. Such an expression cassette is providedwith a plurality of restriction sites and/or recombination sites forinsertion of the polynucleotides to be under the transcriptionalregulation of the regulatory regions. The expression cassette mayadditionally contain a selectable marker gene.

The expression cassette will include in the 5′-3′ direction oftranscription, a transcriptional and translational initiation region(i.e., a promoter), a pesticidal polynucleotide of the invention, and atranscriptional and translational termination region (i.e., terminationregion) functional in the organism of interest, i.e., a plant orbacteria. The promoters of the invention are capable of directing ordriving expression of a coding sequence in a host cell. The regulatoryregions (i.e., promoters, transcriptional regulatory regions, andtranslational termination regions) may be endogenous or heterologous tothe host cell or to each other. As used herein, “heterologous” inreference to a sequence is a sequence that originates from a foreignspecies, or, if from the same species, is substantially modified fromits native form in composition and/or genomic locus by deliberate humanintervention. As used herein, a chimeric gene comprises a codingsequence operably linked to a transcription initiation region that isheterologous to the coding sequence.

Convenient termination regions are available from the Ti-plasmid of A.tumefaciens, such as the octopine synthase and nopaline synthasetermination regions. See also Guerineau et al. (1991) Mol. Gen. Genet.262:141-144; Proudfoot (1991) Cell 64:671-674; Sanfacon et al. (1991)Genes Dev. 5:141-149; Mogen et al. (1990) Plant Cell 2:1261-1272; Munroeet al. (1990) Gene 91:151-158; Ballas et al. (1989) Nucleic Acids Res.17:7891-7903; and Joshi et al. (1987) Nucleic Acids Res. 15:9627-9639.

Additional regulatory signals include, but are not limited to,transcriptional initiation start sites, operators, activators,enhancers, other regulatory elements, ribosomal binding sites, aninitiation codon, termination signals, and the like. See, for example,U.S. Pat. Nos. 5,039,523 and 4,853,331; EPO 0480762A2; Sambrook et al.(1992) Molecular Cloning: A Laboratory Manual, ed. Maniatis et al. (ColdSpring Harbor Laboratory Press, Cold Spring Harbor, N.Y.), hereinafter“Sambrook 11”; Davis et al., eds. (1980) Advanced Bacterial Genetics(Cold Spring Harbor Laboratory Press), Cold Spring Harbor, N.Y., and thereferences cited therein.

In preparing the expression cassette, the various DNA fragments may bemanipulated, so as to provide for the DNA sequences in the properorientation and, as appropriate, in the proper reading frame. Towardthis end, adapters or linkers may be employed to join the DNA fragmentsor other manipulations may be involved to provide for convenientrestriction sites, removal of superfluous DNA, removal of restrictionsites, or the like. For this purpose, in vitro mutagenesis, primerrepair, restriction, annealing, resubstitutions, e.g., transitions andtransversions, may be involved.

A number of promoters can be used in the practice of the invention. Thepromoters can be selected based on the desired outcome. The nucleicacids can be combined with constitutive, inducible, tissue-preferred, orother promoters for expression in the organism of interest. See, forexample, promoters set forth in WO 99/43838 and in U.S. Pat. Nos.8,575,425; 7,790,846; 8,147,856; 8,586832; 7,772,369; 7,534,939;6,072,050; 5,659,026; 5,608,149; 5,608,144; 5,604,121; 5,569,597;5,466,785; 5,399,680; 5,268,463; 5,608,142; and 6,177,611; hereinincorporated by reference.

For expression in plants, constitutive promoters also include CaMV 35Spromoter (Odell et al. (1985) Nature 313:810-812); rice actin (McElroyet al. (1990) Plant Cell 2:163-171); ubiquitin (Christensen et al.(1989) Plant Mol. Biol. 12:619-632 and Christensen et al. (1992) PlantMol. Biol. 18:675-689); pEMU (Last et al. (1991) Theor. Appl. Genet.81:581-588); MAS (Velten et al. (1984) EMBO J. 3:2723-2730). Induciblepromoters include those that drive expression of pathogenesis-relatedproteins (PR proteins), which are induced following infection by apathogen. See, for example, Redolfi et al. (1983) Neth. J. Plant Pathol.89:245-254; Uknes et al. (1992) Plant Cell 4:645-656; and Van Loon(1985) Plant Mol. Virol. 4:111-116; and WO 99/43819, herein incorporatedby reference. Promoters that are expressed locally at or near the siteof pathogen infection may also be used (Marineau et al. (1987) PlantMol. Biol. 9:335-342; Matton et al. (1989) Molecular Plant-MicrobeInteractions 2:325-331; Somsisch et al. (1986) Proc. Natl. Acad. Sci.USA 83:2427-2430; Somsisch et al. (1988) Mol. Gen. Genet. 2:93-98; andYang (1996) Proc. Natl. Acad. Sci. USA 93:14972-14977; Chen et al.(1996) Plant J. 10:955-966; Zhang et al. (1994) Proc. Natl. Acad. Sci.USA 91:2507-2511; Warner et al. (1993) Plant J. 3:191-201; Siebertz etal. (1989) Plant Cell 1:961-968; Cordero et al. (1992) Physiol. Mol.Plant Path. 41:189-200; U.S. Pat. No. 5,750,386 (nematode-inducible);and the references cited therein).

Wound-inducible promoters may be used in the constructions of theinvention. Such wound-inducible promoters include pin II promoter (Ryan(1990) Ann. Rev. Phytopath. 28:425-449; Duan et al. (1996) NatureBiotechnology 14:494-498); wun1 and wun2 (U.S. Pat. No. 5,428,148); win1and win2 (Stanford et al. (1989) Mol. Gen. Genet. 215:200-208); systemin(McGurl et al. (1992) Science 225:1570-1573); WIP1 (Rohmeier et al.(1993) Plant Mol. Biol. 22:783-792; Eckelkamp et al. (1993) FEBS Letters323:73-76); MPI gene (Corderok et al. (1994) Plant J. 6(2):141-150); andthe like, herein incorporated by reference.

Tissue-preferred promoters for use in the invention include those setforth in Yamamoto et al. (1997) Plant J. 12(2):255-265; Kawamata et al.(1997) Plant Cell Physiol. 38(7):792-803; Hansen et al. (1997) Mol. GenGenet. 254(3):337-343; Russell et al. (1997) Transgenic Res.6(2):157-168; Rinehart et al. (1996) Plant Physiol. 112(3):1331-1341;Van Camp et al. (1996) Plant Physiol. 112(2):525-535; Canevascini et al.(1996) Plant Physiol. 112(2):513-524; Yamamoto et al. (1994) Plant CellPhysiol. 35(5):773-778; Lam (1994) Results Probl. Cell Differ.20:181-196; Orozco et al. (1993) Plant Mol Biol. 23(6):1129-1138;Matsuoka et al. (1993) Proc Natl. Acad. Sci. USA 90(20):9586-9590; andGuevara-Garcia et al. (1993) Plant J. 4(3):495-505.

Leaf-preferred promoters include those set forth in Yamamoto et al.(1997) Plant J. 12(2):255-265; Kwon et al. (1994) Plant Physiol.105:357-67; Yamamoto et al. (1994) Plant Cell Physiol. 35(5):773-778;Gotor et al. (1993) Plant J. 3:509-18; Orozco et al. (1993) Plant Mol.Biol. 23(6):1129-1138; and Matsuoka et al. (1993) Proc. Natl. Acad. Sci.USA 90(20):9586-9590.

Root-preferred promoters are known and include those in Hire et al.(1992) Plant Mol. Biol. 20(2):207-218 (soybean root-specific glutaminesynthetase gene); Keller and Baumgartner (1991) Plant Cell3(10):1051-1061 (root-specific control element); Sanger et al. (1990)Plant Mol. Biol. 14(3):433-443 (mannopine synthase (MAS) gene ofAgrobacterium tumefaciens); and Miao et al. (1991) Plant Cell 3(1):11-22(cytosolic glutamine synthetase (GS)); Bogusz et al. (1990) Plant Cell2(7):633-641; Leach and Aoyagi (1991) Plant Science (Limerick)79(1):69-76 (rolC and rolD); Teeri et al. (1989) EMBO J. 8(2):343-350;Kuster et al. (1995) Plant Mol. Biol. 29(4):759-772 (the VfENOD-GRP3gene promoter); and, Capana et al. (1994) Plant Mol. Biol. 25(4):681-691(rolB promoter). See also U.S. Pat. Nos. 5,837,876; 5,750,386;5,633,363; 5,459,252; 5,401,836; 5,110,732; and U.S. Pat. No. 5,023,179.

“Seed-preferred” promoters include both “seed-specific” promoters (thosepromoters active during seed development such as promoters of seedstorage proteins) as well as “seed-germinating” promoters (thosepromoters active during seed germination). See Thompson et al. (1989)BioEssays 10:108. Seed-preferred promoters include, but are not limitedto, Cim1 (cytokinin-induced message); cZ19B1 (maize 19 kDa zein); milps(myo-inositol-l-phosphate synthase) (see WO 00/11177 and U.S. Pat. No.6,225,529). Gamma-zein is an endosperm-specific promoter. Globulin 1(Glb-1) is a representative embryo-specific promoter. For dicots,seed-specific promoters include, but are not limited to, beanβ-phaseolin, napin, β-conglycinin, soybean lectin, cruciferin, and thelike. For monocots, seed-specific promoters include, but are not limitedto, maize 15 kDa zein, 22 kDa zein, 27 kDa zein, gamma-zein, waxy,shrunken 1, shrunken 2, Globulin 1, etc. See also WO 00/12733, whereseed-preferred promoters from end1 and end2 genes are disclosed.

For expression in a bacterial host, promoters that function in bacteriaare well-known in the art. Such promoters include any of the knowncrystal protein gene promoters, including the promoters of any of thepesticidal proteins of the invention, and promoters specific for B.thuringiensis sigma factors. Alternatively, mutagenized or recombinantcrystal protein-encoding gene promoters may be recombinantly engineeredand used to promote expression of the novel gene segments disclosedherein.

The expression cassette can also comprise a selectable marker gene forthe selection of transformed cells. Selectable marker genes are utilizedfor the selection of transformed cells or tissues. Marker genes includegenes encoding antibiotic resistance, such as those encoding neomycinphosphotransferase II (NEO) and hygromycin phosphotransferase (HPT), aswell as genes conferring resistance to herbicidal compounds, such asglufosinate ammonium, bromoxynil, imidazolinones, and2,4-dichlorophenoxyacetate (2,4-D). Additional selectable markers areknown and any can be used. See, for example, U.S. Provisionalapplication 62/094,697, filed on Dec. 19, 2014, and U.S. ProvisionalApplication 62/189,505, filed Jul. 7, 2015, both of which are hereinincorporated by reference in their entirety, which discloses glufosinateresistance sequences that can be employed as selectable markers. See,for example, PCT/US2015/066648, filed on Dec. 18, 2015, hereinincorporated by reference in its entirety, which discloses glufosinateresistance sequences that can be employed as selectable markers.

IV. Methods, Host Cells and Plant Cells

As indicated, DNA constructs comprising nucleotide sequences encodingthe pesticidal proteins or active variants or fragment thereof can beused to transform plants of interest or other organisms of interest.Methods for transformation involve introducing a nucleotide constructinto a plant. By “introducing” is intended to introduce the nucleotideconstruct to the plant or other host cell in such a manner that theconstruct gains access to the interior of a cell of the plant or hostcell. The methods of the invention do not require a particular methodfor introducing a nucleotide construct to a plant or host cell, onlythat the nucleotide construct gains access to the interior of at leastone cell of the plant or the host organism. Methods for introducingnucleotide constructs into plants and other host cells are known in theart including, but not limited to, stable transformation methods,transient transformation methods, and virus-mediated methods.

The methods result in a transformed organisms, such as a plant,including whole plants, as well as plant organs (e.g., leaves, stems,roots, etc.), seeds, plant cells, propagules, embryos and progeny of thesame. Plant cells can be differentiated or undifferentiated (e.g.callus, suspension culture cells, protoplasts, leaf cells, root cells,phloem cells, pollen).

“Transgenic plants” or “transformed plants” or “stably transformed”plants or cells or tissues refers to plants that have incorporated orintegrated a polynucleotide encoding at least one pesticidal polypeptideof the invention. It is recognized that other exogenous or endogenousnucleic acid sequences or DNA fragments may also be incorporated intothe plant cell. Agrobacterium-and biolistic-mediated transformationremain the two predominantly employed approaches. However,transformation may be performed by infection, transfection,microinjection, electroporation, microprojection, biolistics or particlebombardment, electroporation, silica/carbon fibers, ultrasound mediated,PEG mediated, calcium phosphate co-precipitation, polycation DMSOtechnique, DEAE dextran procedure, Agro and viral mediated(Caulimoriviruses, Geminiviruses, RNA plant viruses), liposome mediatedand the like.

Transformation protocols as well as protocols for introducingpolypeptides or polynucleotide sequences into plants may vary dependingon the type of plant or plant cell, i.e., monocot or dicot, targeted fortransformation. Methods for transformation are known in the art andinclude those set forth in U.S. Pat. Nos. 8,575,425; 7,692,068;8,802,934; 7,541,517; each of which is herein incorporated by reference.See, also, Rakoczy-Trojanowska, M. (2002) Cell Mol Biol Lett. 7:849-858;Jones et al. (2005) Plant Methods 1:5; Rivera et al. (2012) Physics ofLife Reviews 9:308-345; Bartlett et al. (2008) Plant Methods 4:1-12;Bates, G. W. (1999) Methods in Molecular Biology 111:359-366; Binns andThomashow (1988) Annual Reviews in Microbiology 42:575-606; Christou, P.(1992) The Plant Journal 2:275-281; Christou, P. (1995) Euphytica85:13-27; Tzfira et al. (2004) TRENDS in Genetics 20:375-383; Yao et al.(2006) Journal of Experimental Botany 57:3737-3746; Zupan and Zambryski(1995) Plant Physiology 107:1041-1047; Jones et al. (2005) Plant Methods1:5;

Transformation may result in stable or transient incorporation of thenucleic acid into the cell. “Stable transformation” is intended to meanthat the nucleotide construct introduced into a host cell integratesinto the genome of the host cell and is capable of being inherited bythe progeny thereof. “Transient transformation” is intended to mean thata polynucleotide is introduced into the host cell and does not integrateinto the genome of the host cell.

Methods for transformation of chloroplasts are known in the art. See,for example, Svab et al. (1990) Proc. Nail. Acad. Sci. USA 87:8526-8530;Svab and Maliga (1993) Proc. Natl. Acad. Sci. USA 90:913-917; Svab andMaliga (1993) EMBO J. 12:601-606. The method relies on particle gundelivery of DNA containing a selectable marker and targeting of the DNAto the plastid genome through homologous recombination. Additionally,plastid transformation can be accomplished by transactivation of asilent plastid-borne transgene by tissue-preferred expression of anuclear-encoded and plastid-directed RNA polymerase. Such a system hasbeen reported in McBride et al. (1994) Proc. Natl. Acad. Sci. USA91:7301-7305.

The cells that have been transformed may be grown into plants inaccordance with conventional ways. See, for example, McCormick et al.(1986) Plant Cell Reports 5:81-84. These plants may then be grown, andeither pollinated with the same transformed strain or different strains,and the resulting hybrid having constitutive expression of the desiredphenotypic characteristic identified. Two or more generations may begrown to ensure that expression of the desired phenotypic characteristicis stably maintained and inherited and then seeds harvested to ensureexpression of the desired phenotypic characteristic has been achieved.In this manner, the present invention provides transformed seed (alsoreferred to as “transgenic seed”) having a nucleotide construct of theinvention, for example, an expression cassette of the invention, stablyincorporated into their genome.

In specific embodiments, the sequences provide herein can be targeted tospecific cite within the genome of the host cell or plant cell. Suchmethods include, but are not limited to, meganucleases designed againstthe plant genomic sequence of interest (D'Halluin et al. 2013 PlantBiotechnol J); CRISPR-Cas9, TALENs, and other technologies for preciseediting of genomes (Feng, et al. Cell Research 23:1229-1232, 2013,Podevin, et al. Trends Biotechnology, online publication, 2013, Wei etal., J Gen Genomics, 2013, Zhang et al (2013) WO 2013/026740); Cre-loxsite-specific recombination (Dale et al. (1995) Plant J 7:649-659;Lyznik, et al. (2007) Transgenic Plant J 1:1-9; FLP-FRT recombination(Li et al. (2009) Plant Physiol 151:1087-1095); Bxb1-mediatedintegration (Yau et al. Plant J (2011) 701:147-166); zinc-fingermediated integration (Wright et al. (2005) Plant J 44:693-705); Cai etal. (2009) Plant Mol Biol 69:699-709); and homologous recombination(Lieberman-Lazarovich and Levy (2011) Methods Mol Biol 701: 51-65);Puchta (2002) Plant Mol Biol 48:173-182).

The sequence provided herein may be used for transformation of any plantspecies, including, but not limited to, monocots and dicots. Examples ofplants of interest include, but are not limited to, corn (maize),sorghum, wheat, sunflower, tomato, crucifers, peppers, potato, cotton,rice, soybean, sugarbeet, sugarcane, tobacco, barley, and oilseed rape,Brassica sp., alfalfa, rye, millet, safflower, peanuts, sweet potato,cassaya, coffee, coconut, pineapple, citrus trees, cocoa, tea, banana,avocado, fig, guava, mango, olive, papaya, cashew, macadamia, almond,oats, vegetables, ornamentals, and conifers.

Vegetables include, but are not limited to, tomatoes, lettuce, greenbeans, lima beans, peas, and members of the genus Curcumis such ascucumber, cantaloupe, and musk melon. Ornamentals include, but are notlimited to, azalea, hydrangea, hibiscus, roses, tulips, daffodils,petunias, carnation, poinsettia, and chrysanthemum. Preferably, plantsof the present invention are crop plants (for example, maize, sorghum,wheat, sunflower, tomato, crucifers, peppers, potato, cotton, rice,soybean, sugarbeet, sugarcane, tobacco, barley, oilseed rape, etc.).

As used herein, the term plant includes plant cells, plant protoplasts,plant cell tissue cultures from which plants can be regenerated, plantcalli, plant clumps, and plant cells that are intact in plants or partsof plants such as embryos, pollen, ovules, seeds, leaves, flowers,branches, fruit, kernels, ears, cobs, husks, stalks, roots, root tips,anthers, and the like. Grain is intended to mean the mature seedproduced by commercial growers for purposes other than growing orreproducing the species. Progeny, variants, and mutants of theregenerated plants are also included within the scope of the invention,provided that these parts comprise the introduced polynucleotides.Further provided is a processed plant product or byproduct that retainsthe sequences disclosed herein, including for example, soymeal.

In another embodiment, the genes encoding the pesticidal proteins can beused to transform insect pathogenic organisms. Such organisms includebaculoviruses, fungi, protozoa, bacteria, and nematodes. Microorganismhosts that are known to occupy the “phytosphere” (phylloplane,phyllosphere, rhizosphere, and/or rhizoplana) of one or more crops ofinterest may be selected. These microorganisms are selected so as to becapable of successfully competing in the particular environment with thewild-type microorganisms, provide for stable maintenance and expressionof the gene expressing the pesticidal protein, and desirably, providefor improved protection of the pesticide from environmental degradationand inactivation.

Such microorganisms include archaea, bacteria, algae, and fungi. Ofparticular interest are microorganisms such as bacteria, e.g., Bacillus,Pseudomonas, Erwinia, Serratia, Klebsiella, Xanthomonas, Streptomyces,Rhizobium, Rhodopseudomonas, Methylius, Agrobacterium, Acetobacter,Lactobacillus, Arthrobacter, Azotobacter, Leuconostoc, and Alcaligenes.Fungi include yeast, e.g., Saccharomyces, Cryptococcus, Kluyveromyces,Sporobolomyces, Rhodotorula, and Aureobasidium. Of particular interestare such phytosphere bacterial species as Pseudomonas syringae,Pseudomonas aeruginosa, Pseudomonas fluorescens, Serratia marcescens,Acetobacter xylinum, Agrobacteria, Rhodopseudomonas spheroides,Xanthomonas campestris, Rhizobium melioti, Alcaligenes entrophus,Clavibacter xyli and Azotobacter vinlandir and phytosphere yeast speciessuch as Rhodotorula rubra, R. glutinis, R. marina, R. aurantiaca,Cryptococcus albidus, C. diffluens, C. laurentii, Saccharomyces rosei,S. pretoriensis, S. cerevisiae, Sporobolomyces rosues, S. odorus,Kluyveromyces veronae, Aureobasidium pollulans, Bacillus thuringiensis,Escherichia coli, Bacillus subtilis, and the like.

Illustrative prokaryotes, both Gram-negative and gram-positive, includeEnterobacteriaceae, such as Escherichia, Erwinia, Shigella, Salmonella,and Proteus; Bacillaceae; Rhizobiceae, such as Rhizobium; Spirillaceae,such as photobacterium, Zymomonas, Serratia, Aeromonas, Vibrio,Desulfovibrio, Spirillum; Lactobacillaceae; Pseudomonadaceae, such asPseudomonas and Acetobacter; Azotobacteraceae and Nitrobacteraceae.Fungi include Phycomycetes and Ascomycetes, e.g., yeast, such asSaccharomyces and Schizosaccharomyces; and Basidiomycetes yeast, such asRhodotorula, Aureobasidium, Sporobolomyces, and the like.

Genes encoding pesticidal proteins can be introduced by means ofelectrotransformation, PEG induced transformation, heat shock,transduction, conjugation, and the like. Specifically, genes encodingthe pesticidal proteins can be cloned into a shuttle vector, forexample, pHT3101 (Lerecius et al. (1989) FEMS Microbiol. Letts. 60:211-218. The shuttle vector pHT3101 containing the coding sequence forthe particular pesticidal protein gene can, for example, be transformedinto the root-colonizing Bacillus by means of electroporation (Lereciuset al. (1989) FEMS Microbiol. Letts. 60: 211-218).

Expression systems can be designed so that pesticidal proteins aresecreted outside the cytoplasm of gram-negative bacteria by fusing anappropriate signal peptide to the amino-terminal end of the pesticidalprotein. Signal peptides recognized by E. coli include the OmpA protein(Ghrayeb et al. (1984) EMBO J, 3: 2437-2442).

Pesticidal proteins and active variants thereof can be fermented in abacterial host and the resulting bacteria processed and used as amicrobial spray in the same manner that Bacillus thuringiensis strainshave been used as insecticidal sprays. In the case of a pesticidalprotein(s) that is secreted from Bacillus, the secretion signal isremoved or mutated using procedures known in the art. Such mutationsand/or deletions prevent secretion of the pesticidal protein(s) into thegrowth medium during the fermentation process. The pesticidal proteinsare retained within the cell, and the cells are then processed to yieldthe encapsulated pesticidal proteins.

Alternatively, the pesticidal proteins are produced by introducingheterologous genes into a cellular host. Expression of the heterologousgene results, directly or indirectly, in the intracellular productionand maintenance of the pesticide. These cells are then treated underconditions that prolong the activity of the toxin produced in the cellwhen the cell is applied to the environment of target pest(s). Theresulting product retains the toxicity of the toxin. These naturallyencapsulated pesticidal proteins may then be formulated in accordancewith conventional techniques for application to the environment hostinga target pest, e.g., soil, water, and foliage of plants. See, forexample U.S. Pat. No. 6,468,523 and U.S. Publication No. 20050138685,and the references cited therein. In the present invention, atransformed microorganism (which includes whole organisms, cells,spore(s), pesticidal protein(s), pesticidal component(s), pest-impactingcomponent(s), mutant(s), living or dead cells and cell components,including mixtures of living and dead cells and cell components, andincluding broken cells and cell components) or an isolated pesticidalprotein can be formulated with an acceptable carrier into a pesticidalor agricultural composition(s) that is, for example, a suspension, asolution, an emulsion, a dusting powder, a dispersible granule, awettable powder, and an emulsifiable concentrate, an aerosol, animpregnated granule, an adjuvant, a coatable paste, and alsoencapsulations in, for example, polymer substances.

Agricultural compositions may comprise a polypeptide, a recombinogenicpolypeptide or a variant or fragment thereof, as disclosed herein. Theagricultural composition disclosed herein may be applied to theenvironment of a plant or an area of cultivation, or applied to theplant, plant part, plant cell, or seed.

Such compositions disclosed above may be obtained by the addition of asurface-active agent, an inert carrier, a preservative, a humectant, afeeding stimulant, an attractant, an encapsulating agent, a binder, anemulsifier, a dye, a UV protectant, a buffer, a flow agent orfertilizers, micronutrient donors, or other preparations that influenceplant growth. One or more agrochemicals including, but not limited to,herbicides, insecticides, fungicides, bactericides, nematicides,molluscicides, acaracides, plant growth regulators, harvest aids, andfertilizers, can be combined with carriers, surfactants or adjuvantscustomarily employed in the art of formulation or other components tofacilitate product handling and application for particular target pests.Suitable carriers and adjuvants can be solid or liquid and correspond tothe substances ordinarily employed in formulation technology, e.g.,natural or regenerated mineral substances, solvents, dispersants,wetting agents, tackifiers, binders, or fertilizers. The activeingredients of the present invention are normally applied in the form ofcompositions and can be applied to the crop area, plant, or seed to betreated. For example, the compositions of the present invention may beapplied to grain in preparation for or during storage in a grain bin orsilo, etc. The compositions of the present invention may be appliedsimultaneously or in succession with other compounds. Methods ofapplying an active ingredient of the present invention or anagrochemical composition of the present invention that contains at leastone of the pesticidal proteins produced by the bacterial strains of thepresent invention include, but are not limited to, foliar application,seed coating, and soil application. The number of applications and therate of application depend on the intensity of infestation by thecorresponding pest.

Suitable surface-active agents include, but are not limited to, anioniccompounds such as a carboxylate of, for example, a metal; a carboxylateof a long chain fatty acid; an N-acylsarcosinate; mono or di-esters ofphosphoric acid with fatty alcohol ethoxylates or salts of such esters;fatty alcohol sulfates such as sodium dodecyl sulfate, sodium octadecylsulfate or sodium cetyl sulfate; ethoxylated fatty alcohol sulfates;ethoxylated alkylphenol sulfates; lignin sulfonates; petroleumsulfonates; alkyl aryl sulfonates such as alkyl-benzene sulfonates orlower alkylnaphthalene sulfonates, e.g., butyl-naphthalene sulfonate;salts of sulfonated naphthalene-formaldehyde condensates; salts ofsulfonated phenol-formaldehyde condensates; more complex sulfonates suchas the amide sulfonates, e.g., the sulfonated condensation product ofoleic acid and N-methyl taurine; or the dialkyl sulfosuccinates, e.g.,the sodium sulfonate of dioctyl succinate. Non-ionic agents includecondensation products of fatty acid esters, fatty alcohols, fatty acidamides or fatty-alkyl- or alkenyl-substituted phenols with ethyleneoxide, fatty esters of polyhydric alcohol ethers, e.g., sorbitan fattyacid esters, condensation products of such esters with ethylene oxide,e.g., polyoxyethylene sorbitar fatty acid esters, block copolymers ofethylene oxide and propylene oxide, acetylenic glycols such as2,4,7,9-tetraethyl-5-decyn-4,7-diol, or ethoxylated acetylenic glycols.Examples of a cationic surface-active agent include, for instance, analiphatic mono-, di-, or polyamine such as an acetate, naphthenate oroleate; or oxygen-containing amine such as an amine oxide ofpolyoxyethylene alkylamine; an amide-linked amine prepared by thecondensation of a carboxylic acid with a di- or polyamine; or aquaternary ammonium salt.

Examples of inert materials include but are not limited to inorganicminerals such as kaolin, phyllosilicates, carbonates, sulfates,phosphates, or botanical materials such as cork, powdered corncobs,peanut hulls, rice hulls, and walnut shells.

The compositions of the present invention can be in a suitable form fordirect application or as a concentrate of primary composition thatrequires dilution with a suitable quantity of water or other diluentbefore application. The pesticidal concentration will vary dependingupon the nature of the particular formulation, specifically, whether itis a concentrate or to be used directly. The composition contains 1 to98% of a solid or liquid inert carrier, and 0 to 50% or 0.1 to 50% of asurfactant. These compositions will be administered at the labeled ratefor the commercial product, for example, about 0.01 lb-5.0 lb. per acrewhen in dry form and at about 0.01 pts.-10 pts. per acre when in liquidform.

In a further embodiment, the compositions, as well as the transformedmicroorganisms and pesticidal proteins, provided herein can be treatedprior to formulation to prolong the pesticidal activity when applied tothe environment of a target pest as long as the pretreatment is notdeleterious to the pesticidal activity. Such treatment can be bychemical and/or physical means as long as the treatment does notdeleteriously affect the properties of the composition(s). Examples ofchemical reagents include but are not limited to halogenating agents;aldehydes such as formaldehyde and glutaraldehyde; anti-infectives, suchas zephiran chloride; alcohols, such as isopropanol and ethanol; andhistological fixatives, such as Bouin's fixative and Helly's fixative(see, for example, Humason (1967) Animal Tissue Techniques (W.H. Freemanand Co.).

In one aspect, pests may be killed or reduced in numbers in a given areaby application of the pesticidal proteins provided herein to the area.Alternatively, the pesticidal proteins may be prophylactically appliedto an environmental area to prevent infestation by a susceptible pest.Preferably the pest ingests, or is contacted with, apesticidally-effective amount of the polypeptide. By“pesticidally-effective amount” is intended an amount of the pesticidethat is able to bring about death to at least one pest, or to noticeablyreduce pest growth, feeding, or normal physiological development. Thisamount will vary depending on such factors as, for example, the specifictarget pests to be controlled, the specific environment, location,plant, crop, or agricultural site to be treated, the environmentalconditions, and the method, rate, concentration, stability, and quantityof application of the pesticidally-effective polypeptide composition.The formulations or compositions may also vary with respect to climaticconditions, environmental considerations, and/or frequency ofapplication and/or severity of pest infestation.

The active ingredients are normally applied in the form of compositionsand can be applied to the crop area, plant, or seed to be treated.Methods are therefore provided for providing to a plant, plant cell,seed, plant part or an area of cultivation, an effective amount of theagricultural composition comprising the polypeptide, recombinogenicpolypeptide or an active variant or fragment thereof. By “effectiveamount” is intended an amount of a protein or composition has pesticidalactivity that is sufficient to kill or control the pest or result in anoticeable reduction in pest growth, feeding, or normal physiologicaldevelopment. Such decreases in numbers, pest growth, feeding or normaldevelopment can comprise any statistically significant decrease,including, for example a decrease of about 5%, 10%, 15%, 20%, 25%, 30%,35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 85%, 90%, 95% or greater.

For example, the compositions may be applied to grain in preparation foror during storage in a grain bin or silo, etc. The compositions may beapplied simultaneously or in succession with other compounds. Methods ofapplying an active ingredient or an agrochemical composition comprisingat least one of the polypeptides, recombinogenic polypeptides orvariants or fragments thereof as disclosed herein, include but are notlimited to, foliar application, seed coating, and soil application.

Methods for increasing plant yield are provided. The methods compriseproviding a plant or plant cell expressing a polynucleotide encoding thepesticidal polypeptide sequence disclosed herein and growing the plantor a seed thereof in a field infested with (or susceptible toinfestation by) a pest against which said polypeptide has pesticidalactivity. In some embodiments, the polypeptide has pesticidal activityagainst a lepidopteran, coleopteran, dipteran, hemipteran, or nematodepest, and said field is infested with a lepidopteran, hemipteran,coleopteran, dipteran, or nematode pest. As defined herein, the “yield”of the plant refers to the quality and/or quantity of biomass producedby the plant. By “biomass” is intended any measured plant product. Anincrease in biomass production is any improvement in the yield of themeasured plant product. Increasing plant yield has several commercialapplications. For example, increasing plant leaf biomass may increasethe yield of leafy vegetables for human or animal consumption.Additionally, increasing leaf biomass can be used to increase productionof plant-derived pharmaceutical or industrial products. An increase inyield can comprise any statistically significant increase including, butnot limited to, at least a 1% increase, at least a 3% increase, at leasta 5% increase, at least a 10% increase, at least a 20% increase, atleast a 30%, at least a 50%, at least a 70%, at least a 100% or agreater increase in yield compared to a plant not expressing thepesticidal sequence. In specific methods, plant yield is increased as aresult of improved pest resistance of a plant expressing a pesticidalprotein disclosed herein. Expression of the pesticidal protein resultsin a reduced ability of a pest to infest or feed.

The plants can also be treated with one or more chemical compositions,including one or more herbicide, insecticides, or fungicides.

Non-limiting embodiments include:

1. An isolated polypeptide having insecticidal activity, comprising

(a) a polypeptide comprising an amino acid sequence selected from thegroup consisting of sequences set forth in SEQ ID NO: 1, 2, 3, 4, 5, 6,7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25,26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43,44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61,62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79,80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97,98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112,113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126,127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140,141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154,155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168,169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182,183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196,197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210,211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224,225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238,239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252,253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266,267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280,281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294,295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308,309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322,323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336,337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350,351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364,365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378,379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392,393, 394, 395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406,407, 408, 409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420,421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434,435, 436, 437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448,449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 461, 462,463, 464, 465, 466, 467, 468, 469, 470, 471, 472, 473, 474, 475, 476,477, 478, 479, 480, 481, 482, 483, 484, 485, 486, 487, 488, 489, 490,491, 492, 493, 494, 495, 496, 497, 498, 499, 500, 501, 502, 503, 504,505, 506, 507, 508, 509, 510, 511, 512, 513, 514, 515, 516, 517, 518,519, 520, 521, 522, 523, 524, and/or 525; or

(b) a polypeptide comprising an amino acid sequence having at least thepercent sequence identity set forth in Table 1 to an amino acid sequenceselected from the group consisting of sequences set forth in SEQ ID NOs:1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20,21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38,39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56,57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74,75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92,93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108,109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122,123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136,137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150,151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164,165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178,179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192,193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206,207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220,221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234,235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248,249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262,263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276,277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290,291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304,305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318,319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332,333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346,347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360,361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374,375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388,389, 390, 391, 392, 393, 394, 395, 396, 397, 398, 399, 400, 401, 402,403, 404, 405, 406, 407, 408, 409, 410, 411, 412, 413, 414, 415, 416,417, 418, 419, 420, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430,431, 432, 433, 434, 435, 436, 437, 438, 439, 440, 441, 442, 443, 444,445, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458,459, 460, 461, 462, 463, 464, 465, 466, 467, 468, 469, 470, 471, 472,473, 474, 475, 476, 477, 478, 479, 480, 481, 482, 483, 484, 485, 486,487, 488, 489, 490, 491, 492, 493, 494, 495, 496, 497, 498, 499, 500,501, 502, 503, 504, 505, 506, 507, 508, 509, 510, 511, 512, 513, 514,515, 516, 517, 518, 519, 520, 521, 522, 523, 524, and/or 525.

2. The polypeptide of embodiment 1, wherein said polypeptide comprisesthe amino acid sequence set forth in SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8,9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26,27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44,45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62,63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80,81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98,99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112,113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126,127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140,141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154,155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168,169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182,183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196,197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210,211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224,225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238,239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252,253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266,267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280,281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294,295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308,309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322,323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336,337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350,351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364,365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378,379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392,393, 394, 395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406,407, 408, 409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420,421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434,435, 436, 437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448,449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 461, 462,463, 464, 465, 466, 467, 468, 469, 470, 471, 472, 473, 474, 475, 476,477, 478, 479, 480, 481, 482, 483, 484, 485, 486, 487, 488, 489, 490,491, 492, 493, 494, 495, 496, 497, 498, 499, 500, 501, 502, 503, 504,505, 506, 507, 508, 509, 510, 511, 512, 513, 514, 515, 516, 517, 518,519, 520, 521, 522, 523, 524, and/or 525.

3. A composition comprising the polypeptide of embodiments 1 or 2.

4. The polypeptide of embodiment 2, further comprising heterologousamino acid sequences.

5. A recombinant nucleic acid molecule that encodes the polypeptide ofembodiment 1, wherein said recombinant nucleic acid molecule is not thenaturally occurring sequence encoding said polypeptide.

6. The recombinant nucleic acid of embodiment 5, wherein said nucleicacid molecule is a synthetic sequence that has been designed forexpression in a plant.

7. The recombinant nucleic acid molecule of embodiment 6, wherein saidnucleic acid molecule is operably linked to a promoter capable ofdirecting expression in a plant cell.

8. The recombinant nucleic acid molecule of embodiment 5, wherein saidnucleic acid molecule is operably linked to a promoter capable ofdirecting expression in a bacteria.

9. A host cell that contains the recombinant nucleic acid molecule ofembodiment 8.

10. The host cell of embodiment 9, wherein said host cell is a bacterialhost cell.

11. A DNA construct comprising a promoter that drives expression in aplant cell operably linked to a recombinant nucleic acid moleculecomprising

(a) a nucleotide sequence that encodes a polypeptide comprising theamino acid sequence of any one of SEQ ID NOs: 1, 2, 3, 4, 5, 6, 7, 8, 9,10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27,28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45,46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63,64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81,82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99,100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113,114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127,128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141,142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155,156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169,170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183,184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197,198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211,212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225,226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239,240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253,254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267,268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281,282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295,296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309,310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323,324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337,338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351,352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365,366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379,380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393,394, 395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406, 407,408, 409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421,422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435,436, 437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449,450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 461, 462, 463,464, 465, 466, 467, 468, 469, 470, 471, 472, 473, 474, 475, 476, 477,478, 479, 480, 481, 482, 483, 484, 485, 486, 487, 488, 489, 490, 491,492, 493, 494, 495, 496, 497, 498, 499, 500, 501, 502, 503, 504, 505,506, 507, 508, 509, 510, 511, 512, 513, 514, 515, 516, 517, 518, 519,520, 521, 522, 523, 524, and/or 525; or,

(b) a nucleotide sequence that encodes a polypeptide comprising an aminoacid sequence having at least the percent sequence identity set forth inTable 1 to an amino acid sequence selected from the group consisting ofsequences set forth in SEQ ID NOs: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11,12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29,30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47,48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65,66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83,84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100,101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114,115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128,129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142,143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156,157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170,171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184,185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198,199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212,213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226,227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240,241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254,255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268,269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282,283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296,297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310,311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324,325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338,339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352,353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366,367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380,381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394,395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406, 407, 408,409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421, 422,423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435, 436,437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449, 450,451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 461, 462, 463, 464,465, 466, 467, 468, 469, 470, 471, 472, 473, 474, 475, 476, 477, 478,479, 480, 481, 482, 483, 484, 485, 486, 487, 488, 489, 490, 491, 492,493, 494, 495, 496, 497, 498, 499, 500, 501, 502, 503, 504, 505, 506,507, 508, 509, 510, 511, 512, 513, 514, 515, 516, 517, 518, 519, 520,521, 522, 523, 524, and/or 525.

12. The DNA construct of embodiment 11, wherein said nucleotide sequenceis a synthetic DNA sequence that has been designed for expression in aplant.

13. A vector comprising the DNA construct of embodiment 11.

14. A host cell that contains the DNA construct of any one ofembodiments 11-13.

15. The host cell of embodiment 14, wherein the host cell is a plantcell.

16. A transgenic plant comprising the host cell of embodiment 15.

17. A composition comprising the host cell of embodiment 10.

18. The composition of embodiment 17, wherein said composition isselected from the group consisting of a powder, dust, pellet, granule,spray, emulsion, colloid, and solution.

19. The composition of embodiment 17, wherein said composition comprisesfrom about 1% to about 99% by weight of said polypeptide.

20. A method for controlling a pest population comprising contactingsaid population with a pesticidal-effective amount of the composition ofembodiment 17.

21. A method for killing a pest population comprising contacting saidpopulation with a pesticidal-effective amount of the composition ofembodiment 17.

22. A method for producing a polypeptide with pesticidal activity,comprising culturing the host cell of embodiment 9 under conditions inwhich the nucleic acid molecule encoding the polypeptide is expressed.

23. A plant having stably incorporated into its genome a DNA constructcomprising a nucleotide sequence that encodes a protein havingpesticidal activity, wherein said nucleotide sequence comprise

(a) a nucleotide sequence that encodes a polypeptide comprising theamino acid sequence of any one of SEQ ID NOs: 1, 2, 3, 4, 5, 6, 7, 8, 9,10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27,28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45,46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63,64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81,82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99,100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113,114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127,128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141,142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155,156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169,170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183,184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197,198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211,212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225,226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239,240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253,254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267,268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281,282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295,296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309,310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323,324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337,338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351,352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365,366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379,380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393,394, 395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406, 407,408, 409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421,422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435,436, 437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449,450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 461, 462, 463,464, 465, 466, 467, 468, 469, 470, 471, 472, 473, 474, 475, 476, 477,478, 479, 480, 481, 482, 483, 484, 485, 486, 487, 488, 489, 490, 491,492, 493, 494, 495, 496, 497, 498, 499, 500, 501, 502, 503, 504, 505,506, 507, 508, 509, 510, 511, 512, 513, 514, 515, 516, 517, 518, 519,520, 521, 522, 523, 524, and/or 525; or,

(b) a nucleotide sequence that encodes a polypeptide comprising an aminoacid sequence having at least the percent sequence identity set forth inTable 1 to an amino acid sequence selected from the group consisting ofsequences set forth in SEQ ID NOs: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11,12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29,30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47,48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65,66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83,84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100,101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114,115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128,129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142,143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156,157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170,171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184,185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198,199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212,213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226,227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240,241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254,255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268,269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282,283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296,297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310,311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324,325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338,339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352,353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366,367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380,381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394,395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406, 407, 408,409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421, 422,423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435, 436,437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449, 450,451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 461, 462, 463, 464,465, 466, 467, 468, 469, 470, 471, 472, 473, 474, 475, 476, 477, 478,479, 480, 481, 482, 483, 484, 485, 486, 487, 488, 489, 490, 491, 492,493, 494, 495, 496, 497, 498, 499, 500, 501, 502, 503, 504, 505, 506,507, 508, 509, 510, 511, 512, 513, 514, 515, 516, 517, 518, 519, 520,521, 522, 523, 524, and/or 525.

24. A transgenic seed of the plant of embodiment 23.

25. A method for protecting a plant from an insect pest, comprisingexpressing in a plant or cell thereof a nucleotide sequence that encodesa pesticidal polypeptide, wherein said nucleotide sequence comprising

(a) a nucleotide sequence that encodes a polypeptide comprising theamino acid sequence of any one of SEQ ID NOs: 1, 2, 3, 4, 5, 6, 7, 8, 9,10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27,28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45,46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63,64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81,82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99,100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113,114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127,128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141,142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155,156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169,170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183,184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197,198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211,212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225,226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239,240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253,254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267,268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281,282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295,296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309,310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323,324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337,338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351,352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365,366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379,380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393,394, 395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406, 407,408, 409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421,422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435,436, 437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449,450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 461, 462, 463,464, 465, 466, 467, 468, 469, 470, 471, 472, 473, 474, 475, 476, 477,478, 479, 480, 481, 482, 483, 484, 485, 486, 487, 488, 489, 490, 491,492, 493, 494, 495, 496, 497, 498, 499, 500, 501, 502, 503, 504, 505,506, 507, 508, 509, 510, 511, 512, 513, 514, 515, 516, 517, 518, 519,520, 521, 522, 523, 524, and/or 525; or,

(b) a nucleotide sequence that encodes a polypeptide comprising an aminoacid sequence having at least the percent sequence identity set forth inTable 1 to an amino acid sequence selected from the group consisting ofsequences set forth in SEQ ID NOs: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11,12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29,30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47,48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65,66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83,84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100,101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114,115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128,129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142,143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156,157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170,171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184,185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198,199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212,213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226,227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240,241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254,255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268,269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282,283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296,297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310,311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324,325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338,339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352,353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366,367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380,381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394,395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406, 407, 408,409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421, 422,423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435, 436,437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449, 450,451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 461, 462, 463, 464,465, 466, 467, 468, 469, 470, 471, 472, 473, 474, 475, 476, 477, 478,479, 480, 481, 482, 483, 484, 485, 486, 487, 488, 489, 490, 491, 492,493, 494, 495, 496, 497, 498, 499, 500, 501, 502, 503, 504, 505, 506,507, 508, 509, 510, 511, 512, 513, 514, 515, 516, 517, 518, 519, 520,521, 522, 523, 524, and/or 525.

26. The method of embodiment 25, wherein said plant produces apesticidal polypeptide having pesticidal against a lepidopteran orcoleopteran pest.

27. A method for increasing yield in a plant comprising growing in afield a plant or seed thereof having stably incorporated into its genomea DNA construct comprising a promoter that drives expression in a plantoperably linked to a nucleotide sequence that encodes a pesticidalpolypeptide, wherein said nucleotide sequence comprises

(a) a nucleotide sequence that encodes a polypeptide comprising theamino acid sequence of any one of SEQ ID NOs: 1, 2, 3, 4, 5, 6, 7, 8, 9,10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27,28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45,46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63,64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81,82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99,100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113,114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127,128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141,142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155,156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169,170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183,184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197,198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211,212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225,226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239,240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253,254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267,268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281,282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295,296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309,310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323,324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337,338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351,352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365,366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379,380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393,394, 395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406, 407,408, 409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421,422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435,436, 437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449,450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 461, 462, 463,464, 465, 466, 467, 468, 469, 470, 471, 472, 473, 474, 475, 476, 477,478, 479, 480, 481, 482, 483, 484, 485, 486, 487, 488, 489, 490, 491,492, 493, 494, 495, 496, 497, 498, 499, 500, 501, 502, 503, 504, 505,506, 507, 508, 509, 510, 511, 512, 513, 514, 515, 516, 517, 518, 519,520, 521, 522, 523, 524, and/or 525; or,

(b) a nucleotide sequence that encodes a polypeptide comprising an aminoacid sequence having at least the percent sequence identity set forth inTable 1 to an amino acid sequence selected from the group consisting ofsequences set forth in SEQ ID NOs: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11,12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29,30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47,48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65,66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83,84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100,101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114,115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128,129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142,143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156,157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170,171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184,185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198,199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212,213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226,227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240,241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254,255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268,269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282,283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296,297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310,311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324,325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338,339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352,353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366,367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380,381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394,395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406, 407, 408,409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421, 422,423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435, 436,437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449, 450,451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 461, 462, 463, 464,465, 466, 467, 468, 469, 470, 471, 472, 473, 474, 475, 476, 477, 478,479, 480, 481, 482, 483, 484, 485, 486, 487, 488, 489, 490, 491, 492,493, 494, 495, 496, 497, 498, 499, 500, 501, 502, 503, 504, 505, 506,507, 508, 509, 510, 511, 512, 513, 514, 515, 516, 517, 518, 519, 520,521, 522, 523, 524, and/or 525.

28. A method of obtaining a polynucleotide that encodes an improvedpolypeptide comprising pesticidal activity is provided, wherein theimproved polypeptide has at least one improved property over any one ofSEQ ID NOs: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17,18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35,36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53,54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71,72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89,90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105,106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119,120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133,134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147,148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161,162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175,176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189,190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203,204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217,218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231,232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245,246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259,260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273,274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287,288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301,302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315,316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329,330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343,344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357,358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371,372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385,386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397, 398, 399,400, 401, 402, 403, 404, 405, 406, 407, 408, 409, 410, 411, 412, 413,414, 415, 416, 417, 418, 419, 420, 421, 422, 423, 424, 425, 426, 427,428, 429, 430, 431, 432, 433, 434, 435, 436, 437, 438, 439, 440, 441,442, 443, 444, 445, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455,456, 457, 458, 459, 460, 461, 462, 463, 464, 465, 466, 467, 468, 469,470, 471, 472, 473, 474, 475, 476, 477, 478, 479, 480, 481, 482, 483,484, 485, 486, 487, 488, 489, 490, 491, 492, 493, 494, 495, 496, 497,498, 499, 500, 501, 502, 503, 504, 505, 506, 507, 508, 509, 510, 511,512, 513, 514, 515, 516, 517, 518, 519, 520, 521, 522, 523, 524, and/or525 comprising:

(a) recombining a plurality of parental polynucleotides comprising SEQID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18,19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36,37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54,55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72,73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90,91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106,107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120,121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134,135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148,149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162,163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176,177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190,191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204,205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218,219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232,233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246,247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260,261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274,275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288,289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302,303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316,317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330,331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344,345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358,359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372,373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386,387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397, 398, 399, 400,401, 402, 403, 404, 405, 406, 407, 408, 409, 410, 411, 412, 413, 414,415, 416, 417, 418, 419, 420, 421, 422, 423, 424, 425, 426, 427, 428,429, 430, 431, 432, 433, 434, 435, 436, 437, 438, 439, 440, 441, 442,443, 444, 445, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456,457, 458, 459, 460, 461, 462, 463, 464, 465, 466, 467, 468, 469, 470,471, 472, 473, 474, 475, 476, 477, 478, 479, 480, 481, 482, 483, 484,485, 486, 487, 488, 489, 490, 491, 492, 493, 494, 495, 496, 497, 498,499, 500, 501, 502, 503, 504, 505, 506, 507, 508, 509, 510, 511, 512,513, 514, 515, 516, 517, 518, 519, 520, 521, 522, 523, 524, and/or 525,or an active variant or fragment thereof to produce a library ofrecombinant polynucleotides encoding recombinant pesticidalpolypeptides;

(b) screening the library to identify a recombinant polynucleotide thatencodes an improved recombinant pesticidal polypeptide that has anenhanced property improved over the parental polynucleotide;

(c) recovering the recombinant polynucleotide that encodes the improvedrecombinant pesticidal polypeptide identified in (b); and,

(d) repeating steps (a), (b) and (c) using the recombinantpolynucleotide recovered in step (c) as one of the plurality of parentalpolynucleotides in repeated step (a).

The following examples are offered by way of illustration and not by wayof limitation.

EXPERIMENTAL Example 1: Discovery of Novel Genes by Sequencing and DNAAnalysis

Microbial cultures were grown in liquid culture in standard laboratorymedia. Cultures were grown to saturation (16 to 24 hours) before DNApreparation. DNA was extracted from bacterial cells by detergent lysis,followed by binding to a silica matrix and washing with an ethanolbuffer. Purified DNA was eluted from the silica matrix with a mildlyalkaline aqueous buffer.

DNA for sequencing was tested for purity and concentration byspectrophotometry. Sequencing libraries were prepared using the NexteraXT library preparation kit according to the manufacturer's protocol.Sequence data was generated on a HiSeq 2000 according to the IlluminaHiSeq 2000 System User Guide protocol.

Sequencing reads were assembled into draft genomes using the CLC BioAssembly Cell software package. Following assembly, gene calls were madeby several methods and resulting gene sequences were interrogated toidentify novel homologs of pesticidal genes. Novel genes were identifiedby BLAST, by domain composition, and by pairwise alignment versus atarget set of pesticidal genes. A summary of such sequences is set forthin Table 1.

Genes identified in the homology search were amplified from bacterialDNA by PCR and cloned into bacterial expression vectors containing fusedin-frame purification tags. Cloned genes were expressed in E. coli andpurified by column chromatography. Purified proteins were assessed ininsect diet bioassay studies to identify active proteins.

Example 2. Heterologous Expression in E. Coli

Each open reading frame set forth in SEQ ID NOs: 1-525 is cloned into anE. coli expression vector containing a maltose binding protein (pMBP).The expression vector is transformed into BL21*RIPL. An LB culturesupplemented with carbenicillin is inoculated with a single colony andgrown overnight at 37° C. using 0.5% of the overnight culture, a freshculture is inoculated and grown to logarithmic phase at 37° C. Theculture is induced using 250 mM IPTG for 18 hours at 16° C. The cellsare pelleted and resuspended in 10 mM Tris pH7.4 and 150 mM NaClsupplemented with protease inhibitors. The protein expression isevaluated by SDS-PAGE.

Example 3. Pesticidal Activity Against Coleopteran and Lepidopteran

Methods

Protein Expression:

Each sequence set forth in SEQ ID NOs: 1-525 is expressed in E. coli asdescribed in Example 2. 400 mL of LB is inoculated and grown to an OD600of 0.6. The culture is induced with 0.25 mM IPTG overnight at 16° C. Thecells are spun down and the cell pellet is resuspend in 5 mL of buffer.The resuspension is sonicated for 2 min on ice.

Bioassay:

Fall army worm (FAW), corn ear worm (CEW), European corn borer (ECB)southwestern corn borer (SWCB), velvetbean caterpillar (VBC) and diamondbacked moth (DBM) eggs are purchased from a commercial insectary (BenzonResearch Inc., Carlisle, Pa.). The FAW, CEW, ECB, VBC and BCW eggs areincubated to the point that eclosion would occur within 12 hrs of theassay setup. SWCB and DBM are introduced to the assay as neonate larvae.Assays are carried out in 24-well trays containing multispecieslepidopteran diet (SOUTHLAND PRODUCTS INCORPORATED, Lake Village, Ark.).Samples of the sonicated lysate are applied to the surface of the diet(diet overlay) and allowed to evaporate and soak into the diet. 100 μlof sonicated lysate is added to the diet surface and dried. The bioassayplates are sealed with a plate sealing film vented with pin holes. Theplates are incubated at 26° C. at 65% RH on a 16:8 day:night cycle in aPercival for 5 days. The assays are assessed for level of mortality,growth inhibition and feeding inhibition.

For the western corn rootworm bioassay, the protein construct/lysate isevaluated in an insect bioassay by dispensing 60 μl volume on the topsurface of diet in wells of 24-well plate (Cellstar, 24-well, GreinerBio One) and allowed to dry. Each well contains 500 μl diet (Marrone etal., 1985). Fifteen to twenty neonate larvae are introduced in each wellusing a fine tip paint brush and the plate is covered with membrane(Viewseal, Greiner Bio One). The bioassay is stored at ambienttemperature and scored for mortality, and/or growth/feeding inhibitionat day 4.

For Colorado Potato Beetle (CPB) a cork bore size No. 8 leaf disk isexcised from potato leaf and is dipped in the protein construct/lysateuntil thoroughly wet and placed on top of filter disk (Millipore, glassfiber filter, 13 mm). Sixty μl dH₂O is added to each filter disk andplaced in each well of 24-well plate (Cellstar, 24-well, Greiner BioOne). The leaf disk is allowed to dry and five to seven first instarlarvae are introduced in each well using a fine tip paint brush. Theplate is covered with membrane (Viewseal, Greiner Bio One) and smallhole is punctured in each well of the membrane. The construct isevaluated with four replicates, and scored for mortality and leaf damageon day 3.

TABLE 3 Seq APG ID VBC FAW CEW BCW ECB SWCB CPB DBM WCRW APG00751.1 2 SS— — NT NT NT NT NT — APG00831.2 6 — — — NT NT NT NT NT — APG00832.1 8 NT— — NT NT NT NT NT — APG00842.1 11 NT — SS NT NT NT NT NT — APG00887.113 NT M, S — NT NT NT NT NT — APG00961.1 15 NT — — NT NT NT NT NT —APG00973.2 18 — — — NT NT NT NT NT — APG01038.1 20 — — — NT NT NT NT NT— APG01063.1 22 NT S — NT NT NT NT NT — APG01092.1 24 — — — NT NT NT NTNT — APG01102.1 26 — — — NT NT NT NT NT — APG01127.0 28 NT — — NT NT NTNT NT — APG01141.1 30 NT M, S M, S NT NT NT NT NT — APG01163.1 32 — — —NT NT NT NT NT 100 APG01165.1 34 — — — NT NT NT NT NT — APG01201.1 36 —— — NT NT NT NT NT — APG01281.1 38 — — — NT NT NT NT NT — APG01385.0 42NT — — NT NT NT NT NT — APG01396.1 44 — — — NT NT NT NT NT — APG01434.247 — — — NT NT NT NT NT — APG01455.0 49 — — — NT NT NT NT NT —APG01516.1 51 NT — — NT NT NT NT NT — APG01549.0 52 SS — — NT NT NT NTNT — APG01617.1 54 — — — NT NT NT NT NT — APG01681.1 56 — — — NT NT NTNT NT 100  APG01686.1 59 — — — NT NT NT NT NT — APG01710.1 61 — — — NTNT NT NT NT — APG01788.1 63 SS SS — NT NT NT NT NT — APG01803.1 65 NT —— NT NT NT NT NT — APG01810.1 67 NT — — NT NT NT NT NT — APG01817.0 68NT — — NT NT NT NT NT — APG01819.1 70 — — — NT NT NT NT NT — APG01860.172 NT — — NT NT NT NT NT — APG01967.1 74 NT — NT NT NT NT NT —APG02071.1 76 NT — — NT NT NT NT NT — APG02105.0 77 NT — — NT NT NT NTNT — APG02110.1 79 — — — NT NT NT NT NT — APG02115.0 80 SS — — NT NT NTNT NT — APG02143.0 81 — — — NT NT NT NT NT — APG02166.0 82 NT — — NT NTNT NT NT — APG02206.1 84 — — — NT NT NT NT NT — APG02221.1 87 — — — NTNT NT NT NT — APG02256.0 88 NT — — NT NT NT NT NT — APG02256.1 89 NT — —NT NT NT NT NT — APG02309.2 92 NT — — NT NT NT NT NT — APG02315.1 94 NT— — NT NT NT NT NT — APG02319.0 95 — — — NT NT NT NT NT — APG02380.1 97NT — NT NT NT NT NT — APG02451.1 99 — — — NT NT NT NT NT 84 APG02475.2102 — — — NT NT NT NT NT — APG02506.1 104 — — — NT NT NT NT NT —APG02511.2 107 NT — — NT NT NT NT NT — APG02587.1 109 NT — — NT NT NT NTNT — APG02592.0 110 NT — — NT NT NT NT NT — APG02656.1 112 NT — — NT NTNT NT NT — APG02659.1 114 NT — — NT NT NT NT NT — APG02671.1 116 — — —NT NT NT NT NT — APG02676.0 117 NT — — NT NT NT NT NT — APG02706.2 120 —— — NT NT NT NT NT — APG02706.6 124 — — — NT NT NT NT NT — APG02714.0125 — — — NT NT NT NT NT 93 APG02718.1 127 NT — — NT NT NT NT NT —APG02767.1 129 NT — — NT NT NT NT NT — APG02941.0 130 — — — NT NT NT NTNT — APG02947.2 133 — — — NT NT NT NT NT — APG02956.0 134 NT — — NT NTNT NT NT — APG02986.0 135 NT — — NT NT NT NT NT — APG03018.2 138 — — —NT NT NT NT NT — APG03054.0 140 NT — — NT NT NT NT NT — APG03056.1 142NT — — NT NT NT NT NT — APG03182.2 146 NT — — NT NT NT NT NT —APG03218.1 148 — — — NT NT NT NT NT — APG03247.1 150 NT — — NT NT NT NTNT — APG03361.0 151 NT — — NT NT NT NT NT — APG03392.1 153 NT — — NT NTNT NT NT — APG03415.0 154 — — — NT NT NT NT NT 86 APG03504.0 155 — — —NT NT NT NT NT 100  APG03505.2 158 — — — NT NT NT NT NT — APG03505.4 160— — — NT NT NT NT NT — APG03505.6 162 — — — NT NT NT NT NT — APG03527.1164 — — — NT NT NT NT NT 62 APG03584.0 165 NT — — NT NT NT NT NT —APG03584.1 166 NT — — NT NT NT NT NT — APG03587.0 167 — — — NT NT NT NTNT — APG03674.1 169 — — — NT NT NT NT NT — APG03738.0 170 NT — — NT NTNT NT NT — APG03758.0 173 NT — — NT NT NT NT NT — APG03778.0 174 NT — —NT NT NT NT NT — APG03789.0 175 NT — — NT NT NT NT NT — APG03826.0 176NT — — NT NT NT NT NT — APG03887.0 177 SS — — NT NT NT NT NT —APG03893.0 178 — — — NT NT NT NT NT — APG03909.1 180 NT — — NT NT NT NTNT — APG03959.0 181 NT — — NT NT NT NT NT — APG04009.0 182 NT S — NT NTNT NT NT — APG04019.1 184 — — — NT NT NT NT NT — APG04103.1 186 NT S —NT NT NT NT NT — APG04111.0 187 — — — NT NT NT NT NT — APG04141.0 188 NT— — NT NT NT NT NT — APG04162.1 190 NT — — NT NT NT NT NT — APG04302.0193 NT — — NT NT NT NT NT — APG04316.1 195 NT — — NT NT NT NT NT —APG04323.0 196 NT — — NT NT NT NT NT — APG04360.0 197 — — — NT NT NT NTNT — APG04439.1 199 NT — — NT NT NT NT NT — APG04442.1 201 NT — — NT NTNT NT NT — APG04476.1 203 — — — NT NT NT NT NT — APG04568.1 205 — — — NTNT NT NT NT — APG04602.1 207 — — — NT NT NT NT NT — APG04651.0 208 — — —NT NT NT NT NT — APG04683.2 211 NT M, S — NT NT NT NT NT 83 APG04687.2214 NT — — NT NT NT NT NT — APG04692.2 217 — — — NT NT NT NT NT —APG04694.1 219 — — — NT NT NT NT NT — APG06866.0 220 — SS — NT NT NT NTNT — APG04786.2 223 NT — — NT NT NT NT NT — APG04794.0 224 — — — NT NTNT NT NT — APG04821.1 226 NT — — NT NT NT NT NT — APG04822.1 228 — — —NT NT NT NT NT — APG04959.0 229 NT — — NT NT NT NT NT — APG04966.1 231NT — — NT NT NT NT NT — APG04977.0 232 — — — NT NT NT NT NT — APG05073.2235 — — — NT NT NT NT NT — APG05078.0 236 NT — — NT NT NT NT NT —APG05095.1 238 — — — NT NT NT NT NT — APG05149.1 241 — — — NT NT NT NTNT — APG05279.2 244 — — — NT NT NT NT NT — APG05300.0 245 NT — — NT NTNT NT NT — APG05346.1 247 NT — — NT NT NT NT NT — APG05388.0 248 NT — —NT NT NT NT NT — APG05408.1 250 NT — — NT NT NT NT NT — APG05414.1 252NT SS SS NT NT NT NT NT — APG05429.1 254 NT — — NT NT NT NT NT —APG05429.2 255 NT — — NT NT NT NT NT — APG05431.1 257 — — — NT NT NT NTNT 100  APG05471.0 258 NT — — NT NT NT NT NT — APG05481.2 261 — — — NTNT NT NT NT — APG05492.0 262 NT — — NT NT NT NT NT 100  APG05508.1 264 —— — NT NT NT NT NT — APG05538.2 267 — — — NT NT NT NT NT — APG05636.0268 NT — — NT NT NT NT NT — APG05643.1 270 NT — — NT NT NT NT NT —APG05662.0 272 NT — — NT NT NT NT NT — APG05725.1 274 — — — NT NT NT NTNT — APG05734.4 279 — — — NT NT NT NT NT — APG05793.0 280 — — — NT NT NTNT NT — APG05834.1 282 NT — — NT NT NT NT NT — APG05878.0 283 — — — NTNT NT NT NT — APG05907.1 285 NT — — NT NT NT NT NT — APG05917.0 286 NT —— NT NT NT NT NT — APG05920.1 288 NT — — NT NT NT NT NT — APG05946.0 289NT — — NT NT NT NT NT — APG05955.0 290 NT — — NT NT NT NT NT —APG05992.0 291 — — — NT NT NT NT NT — APG06027.1 293 NT — — NT NT NT NTNT — APG06049.1 295 NT — — NT NT NT NT NT — APG06079.1 297 NT — — NT NTNT NT NT — APG06195.0 298 NT M, S — NT NT NT NT NT — APG06213.1 300 NT —— NT NT NT NT NT — APG06213.2 301 NT — — NT NT NT NT NT — APG06235.0 302NT — — NT NT NT NT NT — APG06283.0 303 NT S — NT NT NT NT NT —APG06304.1 305 NT — — NT NT NT NT NT — APG06319.1 307 NT — — NT NT NT NTNT — APG06331.1 309 NT — — NT NT NT NT NT — APG06424.0 313 NT — — NT NTNT NT NT — APG06479.1 315 NT — — NT NT NT NT NT — APG06480.1 317 — — —NT NT NT NT NT — APG06493.1 319 NT — — NT NT NT NT NT — APG06496.0 320NT — NT NT NT NT NT — APG06576.1 323 — — — NT NT NT NT NT — APG06578.0324 NT S — NT NT NT NT NT — APG06606.1 326 — — — NT NT NT NT NT —APG06606.2 327 — — — NT NT NT NT NT — APG06677.1 329 NT — — NT NT NT NTNT — APG06697.2 332 NT — — NT NT NT NT NT — APG06705.2 335 — — — NT NTNT NT NT — APG06718.0 336 NT — NT NT NT NT NT — APG06738.1 338 — — — NTNT NT NT NT — APG06774.0 339 NT — — NT NT NT NT NT — APG06791.1 341 NT —— NT NT NT NT NT — APG06883.1 343 NT — — NT NT NT NT NT — APG06898.1 345NT — — NT NT NT NT NT — APG06937.1 347 — — — NT NT NT NT NT — APG06954.1349 NT — — NT NT NT NT NT — APG07054.0 350 NT HM, S — NT NT NT NT NT —APG07057.0 351 NT — — NT NT NT NT NT — APG07094.1 353 NT — — NT NT NT NTNT — APG07107.1 355 — — — NT NT NT NT NT — APG07137.2 358 NT S — NT NTNT NT NT — APG07162.0 359 NT — — NT NT NT NT NT — APG07176.0 360 NT — —NT NT NT NT NT — APG07249.2 363 — — — NT NT NT NT NT — APG07257.0 364 —— — NT NT NT NT NT — APG07307.1 367 NT — — NT NT NT NT NT — APG07310.0368 — — — NT NT NT NT NT — APG07318.1 370 — — — NT NT NT NT NT —APG07322.2 373 — — — NT NT NT NT NT — APG07432.1 376 NT — — NT NT NT NTNT — APG07463.0 377 — — — NT NT NT NT NT — APG07467.1 379 — — — NT NT NTNT NT — APG07487.0 380 — — — NT NT NT NT NT — APG07511.1 382 NT — — NTNT NT NT NT — APG07564.1 384 NT — — NT NT NT NT NT — APG07609.1 386 — —— NT NT NT NT NT — APG07636.0 387 NT — — NT NT NT NT NT — APG07636.1 388NT — — NT NT NT NT NT — APG07664.1 390 NT M, S — NT NT NT NT NT —APG07665.1 392 NT — — NT NT NT NT NT — APG07732.0 393 NT — — NT NT NT NTNT — APG07753.1 395 NT — — NT NT NT NT NT — APG07798.1 397 NT — — NT NTNT NT NT — APG07810.0 398 — — — NT NT NT NT NT — APG07811.1 400 NT — —NT NT NT NT NT — APG07811.2 401 NT — — NT NT NT NT NT — APG07945.1 403 —— — NT NT NT NT NT — APG07969.1 406 NT — — NT NT NT NT NT — APG08021.1410 — — — NT NT NT NT NT — APG08081.2 413 NT — — NT NT NT NT NT —APG08171.0 414 NT — — NT NT NT NT NT — APG08193.1 416 — — — NT NT NT NTNT — APG08207.2 419 — — — NT NT NT NT NT — APG08240.0 420 — — — NT NT NTNT NT — APG08316.0 421 — — — NT NT NT NT NT — APG08335.1 423 NT — — NTNT NT NT NT — APG08339.1 425 NT — — NT NT NT NT NT — APG08370.1 427 — SS— NT NT NT NT NT 100  APG08378.1 429 NT — — NT NT NT NT NT — APG08427.1432 NT — — NT NT NT NT NT — APG08468.0 433 NT — — NT NT NT NT NT —APG08495.0 434 — — — NT NT NT NT NT — APG08526.0 435 NT — — NT NT NT NTNT — APG08576.0 436 NT — — NT NT NT NT NT — APG08577.0 437 NT — — NT NTNT NT NT — APG08624.1 439 NT — — NT NT NT NT NT — APG08625.1 441 NT — —NT NT NT NT NT — APG08639.0 442 NT S SS NT NT NT NT NT — APG08642.2 445— — — NT NT NT NT NT — APG08757.1 447 NT — — NT NT NT NT NT — APG08765.0448 — — — NT NT NT NT NT — APG08791.2 452 — — — NT NT NT NT NT —APG08812.0 453 NT — — NT NT NT NT NT — APG08823.0 454 NT — — NT NT NT NTNT — APG08852.0 455 NT — — NT NT NT NT NT — APG08912.1 457 NT — — NT NTNT NT NT — APG08912.2 458 NT — — NT NT NT NT NT — APG09027.1 460 — — —NT NT NT NT NT 84 APG09028.1 462 NT — — NT NT NT NT NT — APG09048.1 464NT — NT NT NT NT NT — APG09152.1 466 — — — NT NT NT NT NT — APG09158.1468 NT — — NT NT NT NT NT — APG09181.0 469 NT SS SS NT NT NT NT NT —APG09201.0 470 NT SS — NT NT NT NT NT — APG09210.0 471 — — — NT NT NT NTNT — APG09221.0 472 NT — — NT NT NT NT NT — APG09238.2 475 — — — NT NTNT NT NT — APG09266.1 477 NT SS SS NT NT NT NT NT — APG09289.2 480 SSHM, S — NT NT NT NT NT 100  APG09323.1 482 NT — — NT NT NT NT NT —APG09412.1 484 NT — — NT NT NT NT NT — APG09416.0 487 NT — — NT NT NT NTNT — APG09430.1 489 NT — — NT NT NT NT NT — APG09439.0 490 NT — — NT NTNT NT NT — APG09465.1 492 NT S — NT NT NT NT NT — APG09511.1 494 NT — —NT NT NT NT NT — APG09548.1 496 — — — NT NT NT NT NT — APG09675.0 498 NTS — NT NT NT NT NT — APG09773.0 499 NT — — NT NT NT NT NT — APG09812.0500 NT — — NT NT NT NT NT — APG09814.0 501 NT — — NT NT NT NT NT —APG09826.1 503 NT — — NT NT NT NT NT — APG09835.0 504 — — — NT NT NT NTNT — APG09895.1 506 — — — NT NT NT NT NT — APG09901.1 508 NT — — NT NTNT NT NT — APG09901.2 509 NT — — NT NT NT NT NT — APG09903.1 511 — — —NT NT NT NT NT — APG10001.2 514 — — — NT NT NT NT NT — APG01902.1 516 —— — NT NT NT NT NT — APG02996.1 518 — — — NT NT NT NT NT — APG03098.1520 — — — NT NT NT NT NT — APG04517.0 521 — — — NT NT NT NT NT —APG04619.1 523 — — — NT NT NT NT NT — APG06717.1 525 — — — NT NT NT NTNT — NT: Not tested

Example 4. Pesticidal Activity Against Hemipteran

Protein Expression:

Each of the sequences set forth in SEQ ID NOs: 1-525 is expressed in E.coli as described in Example 2. 400 mL of LB is inoculated and grown toan OD600 of 0.6. The culture is induced with 0.25 mM IPTG overnight at16° C. The cells are spun down and the cell pellet is re-suspended in 5mL of buffer. The resuspension is sonicated for 2 min on ice. Mortalityis scored for each sample.

Second instar southern green stink bug (SGSB) are obtained from acommercial insectary (Benzon Research Inc., Carlisle, Pa.). A 50% v/vratio of sonicated lysate sample to 20% sucrose is employed in thebioassay. Stretched parafilm is used as a feeding membrane to expose theSGSB to the diet/sample mixture. The plates are incubated at 25° C.:21°C., 16:8 day:night cycle at 65% RH for 5 days. Mortality is scored foreach sample.

Soybean Aphids (SBA) are originally obtained from Michigan StateUniversity before maintenance in a colony on soybean plants at theAgBiome laboratory. Six adult aphids are added to each well of a 24 wellplate. Protein extracts are provided in liquid artificial diet at a rateof 25% and are sealed with an artificial membrane through which theaphids are able to feed on the diet/sample mixture. The plates are heldin an incubator at 26° C., 60% relative humidity, 16:8 day:night cyclefor 4-5 days. The SBA test uses a combination of mortality, reproductionand feeding to acquire a Combined score which is recorded on day 4 andday 5 following the initiation of the assay. To have an active sample,the combined score must be below 1.5 (scale: 0-3).

For the Lygus hesperus (LH) bioassay, Lygus hesperus are originallyobtained from Ova the Hill Insectary before maintenance in a colony atthe AgBiome laboratory. A 3:1 v/v ratio of protein fractions to 40%sucrose is employed in the bioassay (final concentration of sample is10% sucrose). The sample is loaded into capillary tubes with 4 holesdrilled for access to the sample. A total of six tubes containing 25 μlof sample are assayed on a total of 10 second instar Lygus hesperus. Thetubes are held in small petri dishes and incubated at a 25° C.:21° C.,16:8 day:night cycle at 65% relative humidity for 8 days. Percentmortality is recorded on days 6 and 8. Positive activity in the assay isconsidered mortality above 50%.

TABLE 4 APG Seq ID SGSB SBA LH APG00751.1 2 NT NT NT APG00831.2 6 NT NT− APG00832.1 8 NT NT − APG00842.1 11 NT NT − APG00887.1 13 NT NT −APG00961.1 15 NT NT − APG00973.2 18 NT NT − APG01038.1 20 NT NT NTAPG01063.1 22 NT NT NT APG01092.1 24 NT NT NT APG01102.1 26 NT NT −APG01127.0 28 NT NT − APG01141.1 30 NT NT NT APG01163.1 32 NT NT NTAPG01165.1 34 NT NT − APG01201.1 36 NT NT − APG01281.1 38 NT NT NTAPG01385.0 42 NT NT − APG01396.1 44 NT NT − APG01434.2 47 NT NT −APG01455.0 49 NT NT − APG01516.1 51 NT NT − APG01549.0 52 NT NT NTAPG01617.1 54 NT NT − APG01681.1 56 NT NT − APG01686.1 59 NT NT −APG01710.1 61 NT NT − APG01788.1 63 NT NT NT APG01803.1 65 NT NT −APG01810.1 67 NT NT NT APG01817.0 68 NT NT NT APG01819.1 70 NT NT NTAPG01860.1 72 NT NT NT APG01967.1 74 NT NT NT APG02071.1 76 NT NT +APG02105.0 77 NT NT − APG02110.1 79 NT NT NT APG02115.0 80 NT NT +APG02143.0 81 NT NT − APG02166.0 82 NT NT NT APG02206.1 84 NT NT −APG02221.1 87 NT NT NT APG02256.0 88 NT NT − APG02256.1 89 NT NT −APG02309.2 92 NT NT NT APG02315.1 94 NT NT − APG02319.0 95 NT NT −APG02380.1 97 NT NT NT APG02451.1 99 NT NT NT APG02475.2 102 NT NT −APG02506.1 104 NT NT − APG02511.2 107 NT NT NT APG02587.1 109 NT NT −APG02592.0 110 NT NT NT APG02656.1 112 NT NT − APG02659.1 114 NT NT NTAPG02671.1 116 NT NT − APG02676.0 117 NT NT − APG02706.2 120 NT NT −APG02706.6 124 NT NT − APG02714.0 125 NT NT − APG02718.1 127 NT NT −APG02767.1 129 NT NT − APG02941.0 130 NT NT − APG02947.2 133 NT NT NTAPG02956.0 134 NT NT − APG02986.0 135 NT NT − APG03018.2 138 NT NT −APG03054.0 140 NT NT − APG03056.1 142 NT NT − APG03182.2 146 NT NT −APG03218.1 148 NT NT − APG03247.1 150 NT NT − APG03361.0 151 NT NT −APG03392.1 153 NT NT NT APG03415.0 154 NT NT NT APG03504.0 155 NT NT NTAPG03505.2 158 NT NT − APG03505.4 160 NT NT − APG03505.6 162 NT NT −APG03527.1 164 NT NT − APG03584.0 165 NT NT − APG03584.1 166 NT NT −APG03587.0 167 NT NT − APG03674.1 169 NT NT − APG03738.0 170 NT NT NTAPG03758.0 173 NT NT NT APG03778.0 174 NT NT − APG03789.0 175 NT NT +APG03826.0 176 NT NT NT APG03887.0 177 NT NT NT APG03893.0 178 NT NT −APG03909.1 180 NT NT − APG03959.0 181 NT NT − APG04009.0 182 NT NT NTAPG04019.1 184 NT NT − APG04103.1 186 NT NT − APG04111.0 187 NT NT −APG04141.0 188 NT NT NT APG04162.1 190 NT NT NT APG04302.0 193 NT NT −APG04316.1 195 NT NT NT APG04323.0 196 NT NT NT APG04360.0 197 NT NT −APG04439.1 199 NT NT − APG04442.1 201 NT NT − APG04476.1 203 NT NT −APG04568.1 205 NT NT − APG04602.1 207 NT NT NT APG04651.0 208 NT NT −APG04683.2 211 NT NT NT APG04687.2 214 NT NT − APG04692.2 217 NT NT NTAPG04694.1 219 NT NT NT APG06866.0 220 NT NT NT APG04786.2 223 NT NT −APG04794.0 224 NT NT − APG04821.1 226 NT NT − APG04822.1 228 NT NT −APG04959.0 229 NT NT − APG04966.1 231 NT NT − APG04977.0 232 NT NT −APG05073.2 235 NT NT − APG05078.0 236 NT NT NT APG05095.1 238 NT NT NTAPG05149.1 241 NT NT NT APG05279.2 244 NT NT NT APG05300.0 245 NT NT −APG05346.1 247 NT NT NT APG05388.0 248 NT NT − APG05408.1 250 NT NT NTAPG05414.1 252 NT NT NT APG05429.1 254 NT NT − APG05429.2 255 NT NT −APG05431.1 257 NT NT NT APG05471.0 258 NT NT NT APG05481.2 261 NT NT −APG05492.0 262 NT NT NT APG05508.1 264 NT NT NT APG05538.2 267 NT NT NTAPG05636.0 268 NT NT − APG05643.1 270 NT NT − APG05662.0 272 NT NT −APG05725.1 274 NT NT NT APG05734.4 279 NT NT NT APG05793.0 280 NT NT −APG05834.1 282 NT NT + APG05878.0 283 NT NT − APG05907.1 285 NT NT −APG05917.0 286 NT NT − APG05920.1 288 NT NT − APG05946.0 289 NT NT −APG05955.0 290 NT NT − APG05992.0 291 NT NT − APG06027.1 293 NT NT NTAPG06049.1 295 NT NT − APG06079.1 297 NT NT NT APG06195.0 298 NT NT NTAPG06213.1 300 NT NT NT APG06213.2 301 NT NT NT APG06235.0 302 NT NT −APG06283.0 303 NT NT NT APG06304.1 305 NT NT NT APG06319.1 307 NT NT −APG06331.1 309 NT NT − APG06424.0 313 NT NT NT APG06479.1 315 NT NT −APG06480.1 317 NT NT NT APG06493.1 319 NT NT − APG06496.0 320 NT NT NTAPG06576.1 323 NT NT − APG06578.0 324 NT NT NT APG06606.1 326 NT NT −APG06606.2 327 NT NT − APG06677.1 329 NT NT − APG06697.2 332 NT NT +APG06705.2 335 NT NT − APG06718.0 336 NT NT NT APG06738.1 338 NT NT −APG06774.0 339 NT NT + APG06791.1 341 NT NT − APG06883.1 343 NT NT −APG06898.1 345 NT NT − APG06937.1 347 NT NT − APG06954.1 349 NT NT −APG07054.0 350 NT NT NT APG07057.0 351 NT NT − APG07094.1 353 NT NT NTAPG07107.1 355 NT NT NT APG07137.2 358 NT NT − APG07162.0 359 NT NT −APG07176.0 360 NT NT NT APG07249.2 363 NT NT NT APG07257.0 364 NT NTAPG07307.1 367 NT NT NT APG07310.0 368 NT NT NT APG07318.1 370 NT NT NTAPG07322.2 373 NT NT NT APG07432.1 376 NT NT NT APG07463.0 377 NT NT NTAPG07467.1 379 NT NT − APG07487.0 380 NT NT − APG07511.1 382 NT NT −APG07564.1 384 NT NT NT APG07609.1 386 NT NT − APG07636.0 387 NT NT −APG07636.1 388 NT NT − APG07664.1 390 NT NT NT APG07665.1 392 NT NT −APG07732.0 393 NT NT NT APG07753.1 395 NT NT NT APG07798.1 397 NT NT −APG07810.0 398 NT NT − APG07811.1 400 NT NT NT APG07811.2 401 NT NT NTAPG07945.1 403 NT NT − APG07969.1 406 NT NT NT APG08021.1 410 NT NT NTAPG08081.2 413 NT NT NT APG08171.0 414 NT NT NT APG08193.1 416 NT NT −APG08207.2 419 NT NT − APG08240.0 420 NT NT − APG08316.0 421 NT NT NTAPG08335.1 423 NT NT − APG08339.1 425 NT NT NT APG08370.1 427 NT NT NTAPG08378.1 429 NT NT − APG08427.1 432 NT NT − APG08468.0 433 NT NT NTAPG08495.0 434 NT NT − APG08526.0 435 NT NT NT APG08576.0 436 NT NT NTAPG08577.0 437 NT NT NT APG08624.1 439 NT NT − APG08625.1 441 NT NT −APG08639.0 442 NT NT NT APG08642.2 445 NT NT − APG08757.1 447 NT NT −APG08765.0 448 NT NT − APG08791.2 452 NT NT NT APG08812.0 453 NT NT NTAPG08823.0 454 NT NT NT APG08852.0 455 NT NT − APG08912.1 457 NT NT NTAPG08912.2 458 NT NT NT APG09027.1 460 NT NT − APG09028.1 462 NT NT −APG09048.1 464 NT NT NT APG09152.1 466 NT NT − APG09158.1 468 NT NT NTAPG09181.0 469 NT NT NT APG09201.0 470 NT NT − APG09210.0 471 NT NT −APG09221.0 472 NT NT − APG09238.2 475 NT NT NT APG09266.1 477 NT NT NTAPG09289.2 480 NT NT NT APG09323.1 482 NT NT NT APG09412.1 484 NT NT NTAPG09416.0 487 NT NT − APG09430.1 489 NT NT − APG09439.0 490 NT NT NTAPG09465.1 492 NT NT NT APG09511.1 494 NT NT − APG09548.1 496 NT NT −APG09675.0 498 NT NT NT APG09773.0 499 NT NT NT APG09812.0 500 NT NT NTAPG09814.0 501 NT NT − APG09826.1 503 NT NT NT APG09835.0 504 NT NT −APG09895.1 506 NT NT NT APG09901.1 508 NT NT NT APG09901.2 509 NT NT NTAPG09903.1 511 NT NT − APG10001.2 514 NT NT − APG01902.1 516 NT NT −APG02996.1 518 NT NT − APG03098.1 520 NT NT − APG04517.0 521 NT NT −APG04619.1 523 NT NT − APG06717.1 525 NT NT + NT: Not tested

Example 5. Transformation of Soybean

DNA constructs comprising each of SEQ ID NOs: 1-525 or active variantsor fragments thereof operably linked to a promoter active in a plant arecloned into transformation vectors and introduced into Agrobacterium asdescribed in U.S. Provisional Application No. 62/094,782, filed Dec. 19,2015, herein incorporated by reference in its entirety.

Four days prior to inoculation, several loops of Agrobacterium arestreaked to a fresh plate of YEP* medium supplemented with theappropriate antibiotics** (spectinomycin, chloramphenicol andkanamycin). Bacteria are grown for two days in the dark at 28° C. Aftertwo days, several loops of bacteria are transferred to 3 ml of YEPliquid medium with antibiotics in a 125 ml Erlenmeyer flask. Flasks areplaced on a rotary shaker at 250 RPM at 28° C. overnight. One day beforeinoculation, 2-3 ml of the overnight culture were transferred to 125 mlof YEP with antibiotics in a 500 ml Erlenmeyer flask. Flasks are placedon a rotary shaker at 250 RPM at 28° C. overnight.

Prior to inoculation, the OD of the bacterial culture is checked at OD620. An OD of 0.8-1.0 indicates that the culture is in log phase. Theculture is centrifuged at 4000 RPM for 10 minutes in Oakridge tubes. Thesupernatant is discarded and the pellet is re-suspended in a volume ofSoybean Infection Medium (SI) to achieve the desired OD. The culturesare held with periodic mixing until needed for inoculation.

Two or three days prior to inoculation, soybean seeds are surfacesterilized using chlorine gas. In a fume hood, a petri dish with seedsis place in a bell jar with the lid off. 1.75 ml of 12 N HCl is slowlyadded to 100 ml of bleach in a 250 ml Erlenmeyer flask inside the belljar. The lid is immediately placed on top of the bell jar. Seeds areallowed to sterilize for 14-16 hours (overnight). The top is removedfrom the bell jar and the lid of the petri dish is replaced. The petridish with the surface sterilized is then opened in a laminar flow foraround 30 minutes to disperse any remaining chlorine gas.

Seeds are imbibed with either sterile DI water or soybean infectionmedium (SI) for 1-2 days. Twenty to 30 seeds are covered with liquid ina 100×25 mm petri dish and incubated in the dark at 24° C. Afterimbibition, non-germinating seeds are discarded.

Cotyledonary explants are processed on a sterile paper plate withsterile filter paper dampened using SI medium employing the methods ofU.S. Pat. No. 7,473,822, herein incorporated by reference.

Typically, 16-20 cotyledons are inoculated per treatment. The SI mediumused for holding the explants is discarded and replaced with 25 ml ofAgrobacterium culture (OD 620=0.8-20). After all explants are submerged,the inoculation is carried out for 30 minutes with periodic swirling ofthe dish. After 30 minutes, the Agrobacterium culture is removed.

Co-cultivation plates are prepared by overlaying one piece of sterilepaper onto Soybean Co-cultivation Medium (SCC). Without blotting, theinoculated cotyledons are cultured adaxial side down on the filterpaper. Around 20 explants can be cultured on each plate. The plates aresealed with Parafilm and cultured at 24° C. and around 120 μmoles m-2s-1(in a Percival incubator) for 4-5 days.

After co-cultivation, the cotyledons are washed 3 times in 25 ml ofSoybean Wash Medium with 200 mg/l of cefotaxime and timentin. Thecotyledons are blotted on sterile filter paper and then transferred toSoybean Shoot Induction Medium (SSI). The nodal end of the explant isdepressed slightly into the medium with distal end kept above thesurface at about 45 deg. No more than 10 explants are cultured on eachplate. The plates are wrapped with Micropore tape and cultured in thePercival at 24° C. and around 120 μmoles m-2s-1.

The explants are transferred to fresh SSI medium after 14 days. Emergingshoots from the shoot apex and cotyledonary node are discarded. Shootinduction is continued for another 14 days under the same conditions.

After 4 weeks of shoot induction, the cotyledon is separated from thenodal end and a parallel cut is made underneath the area of shootinduction (shoot pad). The area of the parallel cut is placed on SoybeanShoot Elongation Medium (SSE) and the explants cultured in the Percivalat 24° C. and around 120 umoles m-2s-1. This step is repeated every twoweeks for up to 8 weeks as long as shoots continue to elongate.

When shoots reach a length of 2-3 cm, they are transferred to SoybeanRooting Medium (SR) in a Plantcon vessel and incubated under the sameconditions for 2 weeks or until roots reach a length of around 3-4 cm.After this, plants are transferred to soil.

Note, all media mentioned for soybean transformation are found in Paz etal. (2010) Agrobacterium-mediated transformation of soybean and recoveryof transgenic soybean plants; Plant Transformation Facility of IowaState University, which is herein incorporated by reference in itsentirety. (See, agron-www.agron.iastate.edu/ptf/protocol/Soybean.pdf.)

Example 6. Transformation of Maize

Maize ears are best collected 8-12 days after pollination. Embryos areisolated from the ears, and those embryos 0.8-1.5 mm in size arepreferred for use in transformation. Embryos are plated scutellumside-up on a suitable incubation media, such as DN62A5S media (3.98 g/LN6 Salts; 1 mL/L (of 1000.times. Stock) N6 Vitamins; 800 mg/LL-Asparagine; 100 mg/L Myo-inositol; 1.4 g/L L-Proline; 100 mg/LCasamino acids; 50 g/L sucrose; 1 mL/L (of 1 mg/mL Stock) 2,4-D).However, media and salts other than DN62A5S are suitable and are knownin the art. Embryos are incubated overnight at 25° C. in the dark.However, it is not necessary per se to incubate the embryos overnight.

The resulting explants are transferred to mesh squares (30-40 perplate), transferred onto osmotic media for about 30-45 minutes, thentransferred to a beaming plate (see, for example, PCT Publication No.WO/0138514 and U.S. Pat. No. 5,240,842). DNA constructs designed toexpress the GRG proteins of the present invention in plant cells areaccelerated into plant tissue using an aerosol beam accelerator, usingconditions essentially as described in PCT Publication No. WO/0138514.After beaming, embryos are incubated for about 30 min on osmotic media,and placed onto incubation media overnight at 25° C. in the dark. Toavoid unduly damaging beamed explants, they are incubated for at least24 hours prior to transfer to recovery media. Embryos are then spreadonto recovery period media, for about 5 days, 25° C. in the dark, thentransferred to a selection media. Explants are incubated in selectionmedia for up to eight weeks, depending on the nature and characteristicsof the particular selection utilized. After the selection period, theresulting callus is transferred to embryo maturation media, until theformation of mature somatic embryos is observed. The resulting maturesomatic embryos are then placed under low light, and the process ofregeneration is initiated by methods known in the art. The resultingshoots are allowed to root on rooting media, and the resulting plantsare transferred to nursery pots and propagated as transgenic plants.

Example 7. Pesticidal Activity Against Nematodes

Heterodera glycine's (Soybean Cyst Nematode) In-Vitro Assay.

Soybean Cyst Nematodes are dispensed into a 96 well assay plate with atotal volume of 100 uls and 100 J2 per well. The protein of interest asset forth in any one of SEQ ID NOs: 1-525 is dispensed into the wellsand held at room temperature for assessment. Finally the 96 well platecontaining the SCN J2 is analyzed for motility. Data is reported as %inhibition as compared to the controls. Hits are defined as greater orequal to 70% inhibition.

Heterodera glycine's (Soybean Cyst Nematode) On-Plant Assay

Soybean plants expressing one or more of SEQ ID NOs: 1-525 are generatedas described elsewhere herein. A 3-week-old soybean cutting isinoculated with 5000 SCN eggs per plant. This infection is held for 70days and then harvested for counting of SCN cyst that has developed onthe plant. Data is reported as % inhibition as compared to the controls.Hits are defined as greater or equal to 90% inhibition.

Meloidogyne incognita (Root-Knot Nematode) In-Vitro Assay

Root-Knot Nematodes are dispensed into a 96 well assay plate with atotal volume of 100 μls and 100 J2 per well. The protein of interestcomprising any one of SEQ ID NOs: 1-525 is dispensed into the wells andheld at room temperature for assessment. Finally the 96 well platecontaining the RKN J2 is analyzed for motility. Data is reported as %inhibition as compared to the controls. Hits are defined as greater orequal to 70% inhibition.

Meloidogyne incognita (Root-Knot Nematode) On-Plant Assay

Soybean plants expressing one or more of SEQ ID NOs: 1-525 are generatedas described elsewhere herein. A 3-week-old soybean is inoculated with5000 RKN eggs per plant. This infection is held for 70 days and thenharvested for counting of RKN eggs that have developed in the plant.Data is reported as % inhibition as compared to the controls. Hits aredefined as greater or equal to 90% inhibition.

Example 8. Additional Assays for Pesticidal Activity

The various polypeptides set forth in SEQ ID NOs: 1-525 can be tested toact as a pesticide upon a pest in a number of ways. One such method isto perform a feeding assay. In such a feeding assay, one exposes thepest to a sample containing either compounds to be tested or controlsamples. Often this is performed by placing the material to be tested,or a suitable dilution of such material, onto a material that the pestwill ingest, such as an artificial diet. The material to be tested maybe composed of a liquid, solid, or slurry. The material to be tested maybe placed upon the surface and then allowed to dry. Alternatively, thematerial to be tested may be mixed with a molten artificial diet, andthen dispensed into the assay chamber. The assay chamber may be, forexample, a cup, a dish, or a well of a microtiter plate.

Assays for sucking pests (for example aphids) may involve separating thetest material from the insect by a partition, ideally a portion that canbe pierced by the sucking mouth parts of the sucking insect, to allowingestion of the test material. Often the test material is mixed with afeeding stimulant, such as sucrose, to promote ingestion of the testcompound.

Other types of assays can include microinjection of the test materialinto the mouth, or gut of the pest, as well as development of transgenicplants, followed by test of the ability of the pest to feed upon thetransgenic plant. Plant testing may involve isolation of the plant partsnormally consumed, for example, small cages attached to a leaf, orisolation of entire plants in cages containing insects.

Other methods and approaches to assay pests are known in the art, andcan be found, for example in Robertson and Preisler, eds. (1992)Pesticide bioassays with arthropods, CRC, Boca Raton, Fla.Alternatively, assays are commonly described in the journals ArthropodManagement Tests and Journal of Economic Entomology or by discussionwith members of the Entomological Society of America (ESA). Any one ofSEQ ID NOs: 1-525 can be expressed and employed in an assay as set forthin Examples 3 and 4, herein.

All publications and patent applications mentioned in the specificationare indicative of the level of skill of those skilled in the art towhich this invention pertains. All publications and patent applicationsare herein incorporated by reference to the same extent as if eachindividual publication or patent application was specifically andindividually indicated to be incorporated by reference.

Although the foregoing invention has been described in some detail byway of illustration and example for purposes of clarity ofunderstanding, it will be obvious that certain changes and modificationsmay be practiced within the scope of the appended claims.

That which is claimed is:
 1. A recombinant polypeptide comprising: (a) apolypeptide comprising an amino acid sequence selected from the groupconsisting of sequences set forth in SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8,9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26,27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44,45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62,63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80,81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98,99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112,113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126,127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140,141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154,155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168,169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182,183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196,197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210,211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224,225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238,239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252,253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266,267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280,281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294,295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308,309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322,323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336,337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350,351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364,365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378,379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392,393, 394, 395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406,407, 408, 409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420,421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434,435, 436, 437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448,449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 461, 462,463, 464, 465, 466, 467, 468, 469, 470, 471, 472, 473, 474, 475, 476,477, 478, 479, 480, 481, 482, 483, 484, 485, 486, 487, 488, 489, 490,491, 492, 493, 494, 495, 496, 497, 498, 499, 500, 501, 502, 503, 504,505, 506, 507, 508, 509, 510, 511, 512, 513, 514, 515, 516, 517, 518,519, 520, 521, 522, 523, 524, and/or 525, wherein said polypeptidefurther comprises a heterologous amino acid sequence chemically linkedto said polypeptide; or (b) a polypeptide comprising an amino acidsequence having at least 90% sequence identity to an amino acid sequenceselected from the group consisting of sequences set forth in SEQ ID NOs:1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20,21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38,39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56,57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74,75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92,93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108,109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122,123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136,137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150,151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164,165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178,179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192,193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206,207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220,221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234,235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248,249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262,263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276,277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290,291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304,305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318,319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332,333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346,347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360,361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374,375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388,389, 390, 391, 392, 393, 394, 395, 396, 397, 398, 399, 400, 401, 402,403, 404, 405, 406, 407, 408, 409, 410, 411, 412, 413, 414, 415, 416,417, 418, 419, 420, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430,431, 432, 433, 434, 435, 436, 437, 438, 439, 440, 441, 442, 443, 444,445, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458,459, 460, 461, 462, 463, 464, 465, 466, 467, 468, 469, 470, 471, 472,473, 474, 475, 476, 477, 478, 479, 480, 481, 482, 483, 484, 485, 486,487, 488, 489, 490, 491, 492, 493, 494, 495, 496, 497, 498, 499, 500,501, 502, 503, 504, 505, 506, 507, 508, 509, 510, 511, 512, 513, 514,515, 516, 517, 518, 519, 520, 521, 522, 523, 524, and/or 525, whereinsaid polypeptide has pesticidal activity; and wherein said polypeptidefurther comprises a heterologous amino acid sequence chemically linkedto said polypeptide.
 2. A composition comprising the polypeptide ofclaim
 1. 3. A nucleic acid molecule encoding a polypeptide comprising:(a) a polypeptide comprising an amino acid sequence selected from thegroup consisting of sequences set forth in SEQ ID NOs: 1, 2, 3, 4, 5, 6,7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25,26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43,44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61,62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79,80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97,98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112,113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126,127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140,141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154,155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168,169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182,183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196,197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210,211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224,225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238,239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252,253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266,267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280,281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294,295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308,309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322,323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336,337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350,351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364,365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378,379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392,393, 394, 395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406,407, 408, 409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420,421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434,435, 436, 437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448,449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 461, 462,463, 464, 465, 466, 467, 468, 469, 470, 471, 472, 473, 474, 475, 476,477, 478, 479, 480, 481, 482, 483, 484, 485, 486, 487, 488, 489, 490,491, 492, 493, 494, 495, 496, 497, 498, 499, 500, 501, 502, 503, 504,505, 506, 507, 508, 509, 510, 511, 512, 513, 514, 515, 516, 517, 518,519, 520, 521, 522, 523, 524, and/or 525; or (b) a polypeptidecomprising an amino acid sequence having at least 90% sequence identityto an amino acid sequence selected from the group consisting ofsequences set forth in SEQ ID NOs: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11,12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29,30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47,48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65,66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83,84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100,101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114,115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128,129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142,143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156,157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170,171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184,185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198,199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212,213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226,227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240,241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254,255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268,269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282,283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296,297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310,311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324,325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338,339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352,353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366,367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380,381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394,395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406, 407, 408,409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421, 422,423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435, 436,437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449, 450,451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 461, 462, 463, 464,465, 466, 467, 468, 469, 470, 471, 472, 473, 474, 475, 476, 477, 478,479, 480, 481, 482, 483, 484, 485, 486, 487, 488, 489, 490, 491, 492,493, 494, 495, 496, 497, 498, 499, 500, 501, 502, 503, 504, 505, 506,507, 508, 509, 510, 511, 512, 513, 514, 515, 516, 517, 518, 519, 520,521, 522, 523, 524, and/or 525, wherein said polypeptide has pesticidalactivity; and wherein said nucleic acid molecule is operably linked to aheterologous promoter.
 4. The nucleic acid of claim 3, wherein saidheterologous promoter is capable of directing expression in a plant. 5.The nucleic acid of claim 3, wherein said heterologous promoter iscapable of directing expression in a bacterial cell.
 6. A host cellcomprising the nucleic acid molecule of claim
 3. 7. The host cell ofclaim 6, wherein said host cell is a bacterial host cell.
 8. A vectorcomprising a heterologous promoter that drives expression in a plantcell operably linked to a nucleic acid molecule comprising (a) anucleotide sequence that encodes a polypeptide comprising the amino acidsequence of any one of SEQ ID NOs: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11,12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29,30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47,48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65,66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83,84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100,101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114,115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128,129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142,143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156,157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170,171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184,185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198,199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212,213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226,227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240,241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254,255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268,269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282,283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296,297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310,311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324,325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338,339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352,353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366,367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380,381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394,395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406, 407, 408,409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421, 422,423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435, 436,437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449, 450,451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 461, 462, 463, 464,465, 466, 467, 468, 469, 470, 471, 472, 473, 474, 475, 476, 477, 478,479, 480, 481, 482, 483, 484, 485, 486, 487, 488, 489, 490, 491, 492,493, 494, 495, 496, 497, 498, 499, 500, 501, 502, 503, 504, 505, 506,507, 508, 509, 510, 511, 512, 513, 514, 515, 516, 517, 518, 519, 520,521, 522, 523, 524, and/or 525; or, (b) a nucleotide sequence thatencodes a polypeptide comprising an amino acid sequence having at least90% sequence identity to an amino acid sequence selected from the groupconsisting of sequences set forth in SEQ ID NOs: 1, 2, 3, 4, 5, 6, 7, 8,9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26,27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44,45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62,63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80,81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98,99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112,113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126,127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140,141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154,155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168,169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182,183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196,197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210,211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224,225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238,239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252,253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266,267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280,281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294,295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308,309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322,323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336,337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350,351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364,365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378,379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392,393, 394, 395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406,407, 408, 409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420,421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434,435, 436, 437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448,449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 461, 462,463, 464, 465, 466, 467, 468, 469, 470, 471, 472, 473, 474, 475, 476,477, 478, 479, 480, 481, 482, 483, 484, 485, 486, 487, 488, 489, 490,491, 492, 493, 494, 495, 496, 497, 498, 499, 500, 501, 502, 503, 504,505, 506, 507, 508, 509, 510, 511, 512, 513, 514, 515, 516, 517, 518,519, 520, 521, 522, 523, 524, and/or 525, wherein said polypeptide haspesticidal activity.
 9. The vector of claim 8, wherein said nucleotidesequence is a synthetic DNA sequence that has been designed forexpression in a plant.
 10. A host cell comprising the vector of claim 8.11. A composition comprising the host cell of claim
 10. 12. A method forcontrolling a pest population comprising contacting said pest populationwith a pesticidal-effective amount of the composition of claim
 11. 13. Amethod for producing a polypeptide with pesticidal activity, comprisingculturing the host cell of claim 6 under conditions in which the nucleicacid molecule encoding the polypeptide is expressed.
 14. A plant havingstably incorporated into its genome a DNA construct comprising anucleotide sequence that encodes a polypeptide, wherein said polypeptidecomprises: (a) a polypeptide comprising the amino acid sequence of anyone of SEQ ID NOs: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15,16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33,34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51,52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69,70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87,88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104,105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118,119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132,133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146,147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160,161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174,175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188,189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202,203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216,217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230,231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244,245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258,259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272,273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286,287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300,301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314,315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328,329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342,343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356,357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370,371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384,385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397, 398,399, 400, 401, 402, 403, 404, 405, 406, 407, 408, 409, 410, 411, 412,413, 414, 415, 416, 417, 418, 419, 420, 421, 422, 423, 424, 425, 426,427, 428, 429, 430, 431, 432, 433, 434, 435, 436, 437, 438, 439, 440,441, 442, 443, 444, 445, 446, 447, 448, 449, 450, 451, 452, 453, 454,455, 456, 457, 458, 459, 460, 461, 462, 463, 464, 465, 466, 467, 468,469, 470, 471, 472, 473, 474, 475, 476, 477, 478, 479, 480, 481, 482,483, 484, 485, 486, 487, 488, 489, 490, 491, 492, 493, 494, 495, 496,497, 498, 499, 500, 501, 502, 503, 504, 505, 506, 507, 508, 509, 510,511, 512, 513, 514, 515, 516, 517, 518, 519, 520, 521, 522, 523, 524,and/or 525; or, (b) a polypeptide comprising an amino acid sequencehaving at least 90% identity to an amino acid sequence selected from thegroup consisting of sequences set forth in SEQ ID NOs: 1, 2, 3, 4, 5, 6,7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25,26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43,44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61,62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79,80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97,98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112,113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126,127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140,141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154,155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168,169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182,183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196,197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210,211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224,225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238,239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252,253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266,267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280,281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294,295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308,309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322,323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336,337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350,351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364,365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378,379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392,393, 394, 395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406,407, 408, 409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420,421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434,435, 436, 437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448,449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 461, 462,463, 464, 465, 466, 467, 468, 469, 470, 471, 472, 473, 474, 475, 476,477, 478, 479, 480, 481, 482, 483, 484, 485, 486, 487, 488, 489, 490,491, 492, 493, 494, 495, 496, 497, 498, 499, 500, 501, 502, 503, 504,505, 506, 507, 508, 509, 510, 511, 512, 513, 514, 515, 516, 517, 518,519, 520, 521, 522, 523, 524, and/or 525, wherein said polypeptide haspesticidal activity.
 15. A transgenic seed of the plant of claim 14,wherein said transgenic seed comprises in its genome the DNA construct.16. A method for protecting a plant from an insect pest, comprisingexpressing in a plant or cell thereof a nucleotide sequence that encodesa polypeptide, wherein said polypeptide comprises: (a) a polypeptidecomprising the amino acid sequence of any one of SEQ ID NOs: 1, 2, 3, 4,5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23,24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41,42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59,60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77,78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95,96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110,111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124,125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138,139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152,153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166,167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180,181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194,195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208,209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222,223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236,237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250,251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264,265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278,279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292,293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306,307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320,321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334,335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348,349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362,363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376,377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390,391, 392, 393, 394, 395, 396, 397, 398, 399, 400, 401, 402, 403, 404,405, 406, 407, 408, 409, 410, 411, 412, 413, 414, 415, 416, 417, 418,419, 420, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432,433, 434, 435, 436, 437, 438, 439, 440, 441, 442, 443, 444, 445, 446,447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460,461, 462, 463, 464, 465, 466, 467, 468, 469, 470, 471, 472, 473, 474,475, 476, 477, 478, 479, 480, 481, 482, 483, 484, 485, 486, 487, 488,489, 490, 491, 492, 493, 494, 495, 496, 497, 498, 499, 500, 501, 502,503, 504, 505, 506, 507, 508, 509, 510, 511, 512, 513, 514, 515, 516,517, 518, 519, 520, 521, 522, 523, 524, and/or 525; or, (b) apolypeptide comprising an amino acid sequence having at least 90%sequence identity to an amino acid sequence selected from the groupconsisting of sequences set forth in SEQ ID NOs: 1, 2, 3, 4, 5, 6, 7, 8,9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26,27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44,45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62,63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80,81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98,99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112,113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126,127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140,141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154,155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168,169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182,183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196,197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210,211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224,225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238,239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252,253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266,267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280,281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294,295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308,309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322,323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336,337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350,351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364,365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378,379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392,393, 394, 395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406,407, 408, 409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420,421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434,435, 436, 437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448,449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 461, 462,463, 464, 465, 466, 467, 468, 469, 470, 471, 472, 473, 474, 475, 476,477, 478, 479, 480, 481, 482, 483, 484, 485, 486, 487, 488, 489, 490,491, 492, 493, 494, 495, 496, 497, 498, 499, 500, 501, 502, 503, 504,505, 506, 507, 508, 509, 510, 511, 512, 513, 514, 515, 516, 517, 518,519, 520, 521, 522, 523, 524, and/or 525, wherein said polypeptide haspesticidal activity.
 17. The method of claim 16, wherein said pesticidalpolypeptide has pesticidal activity against a lepidopteran orcoleopteran pest.
 18. A method for increasing yield in a plantcomprising growing in a field a plant or seed thereof having stablyincorporated into its genome a DNA construct comprising a promoter thatdrives expression in a plant operably linked to a nucleotide sequencethat encodes a polypeptide, wherein said nucleotide sequence comprises:(a) a nucleotide sequence that encodes a polypeptide comprising theamino acid sequence of any one of SEQ ID NOs: 1, 2, 3, 4, 5, 6, 7, 8, 9,10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27,28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45,46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63,64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81,82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99,100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113,114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127,128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141,142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155,156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169,170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183,184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197,198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211,212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225,226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239,240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253,254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267,268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281,282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295,296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309,310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323,324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337,338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351,352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365,366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379,380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393,394, 395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406, 407,408, 409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421,422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435,436, 437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449,450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 461, 462, 463,464, 465, 466, 467, 468, 469, 470, 471, 472, 473, 474, 475, 476, 477,478, 479, 480, 481, 482, 483, 484, 485, 486, 487, 488, 489, 490, 491,492, 493, 494, 495, 496, 497, 498, 499, 500, 501, 502, 503, 504, 505,506, 507, 508, 509, 510, 511, 512, 513, 514, 515, 516, 517, 518, 519,520, 521, 522, 523, 524, and/or 525; or, (b) a nucleotide sequence thatencodes a polypeptide comprising an amino acid sequence having at least90% sequence identity to an amino acid sequence selected from the groupconsisting of sequences set forth in SEQ ID NOs: 1, 2, 3, 4, 5, 6, 7, 8,9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26,27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44,45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62,63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80,81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98,99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112,113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126,127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140,141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154,155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168,169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182,183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196,197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210,211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224,225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238,239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252,253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266,267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280,281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294,295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308,309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322,323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336,337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350,351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364,365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378,379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392,393, 394, 395, 396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406,407, 408, 409, 410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420,421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434,435, 436, 437, 438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448,449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 461, 462,463, 464, 465, 466, 467, 468, 469, 470, 471, 472, 473, 474, 475, 476,477, 478, 479, 480, 481, 482, 483, 484, 485, 486, 487, 488, 489, 490,491, 492, 493, 494, 495, 496, 497, 498, 499, 500, 501, 502, 503, 504,505, 506, 507, 508, 509, 510, 511, 512, 513, 514, 515, 516, 517, 518,519, 520, 521, 522, 523, 524, and/or 525, wherein said polypeptide haspesticidal activity.